LOCUS       CP056611                4072 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00320 plasmid pRHBSTW-00320_4,
            complete sequence.
ACCESSION   CP056611
VERSION     CP056611.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148575
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00320
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.5146007789634x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:38:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,449
            CDSs (total)                      :: 5,326
            Genes (coding)                    :: 5,106
            CDSs (with protein)               :: 5,106
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 96 of 220
            Pseudo Genes (incomplete)         :: 119 of 220
            Pseudo Genes (internal stop)      :: 45 of 220
            Pseudo Genes (multiple problems)  :: 36 of 220
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00320"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00320_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(104..2263)
                     /locus_tag="HV158_27230"
     CDS             complement(104..2263)
                     /locus_tag="HV158_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLV28425.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            2442..2696
                     /locus_tag="HV158_27235"
     CDS             2442..2696
                     /locus_tag="HV158_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLV28426.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            3228..3920
                     /locus_tag="HV158_27240"
     CDS             3228..3920
                     /locus_tag="HV158_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLV28427.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1906..2264)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          2265..2441
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3229..3920
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(108..2350)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttgcattat gtgtgtgctt cttgcattca tccctgtcga tgaacataga agaacatcga
       61 cgaacataac acgatgttaa acaaaacaaa atattgttca cattcactgt gaccaaccgc
      121 gatcatgtct cctctcggtg gtgtgtgttc ttgcatcttc ctcgcgccgc cattcatccc
      181 aggtacgatt gaaacccaga gagcggaaca gatgatccgg ctgtttttct ggtgtgttga
      241 cggcagcaaa aagcggtgcc tcacgggttt tcagatgaaa ctccataagt tccggcgcag
      301 gtgatatgtg gctgtctgca tcgtgattta cggcaacacc ataccctgca taccgtaacc
      361 ccttatcggt cagtgcactg atgaccagct ggctgttatc ggctgaggga acggcaaacg
      421 cgggatacag cgtaccatta agccggacaa caccgcccag agagtcatcc acgcccatga
      481 ccagcaaaag ctgaagctgg cgggtttccc ggtcgacaga actcattacc ggcatcagcg
      541 tcggtagcag acgtccctgc gtaaaatcca ccttccttgc tgccagtgat ggcatggctg
      601 gctggcggtc ttctccaaga cagaaagact ccggccatgt cttcggctgg cggagattgc
      661 gggtatattg cggccatgtc tggcaaacct gcgtcagaag ttgctcaaag gtcgcagagg
      721 aataaacacg ttgcagtggt ggatgttgac cgatttcgct tcggtcacag atataaccac
      781 tcctgtcacg cattaaatca acacgctcaa agattctggt gcggtatttc tccagctcgt
      841 tcccccagag cgtgtattct ccgttccggt tgtgaagcag aaggtaacag gtcatttccc
      901 cgttaccagg catgggggcc tcgccatacc ccgtaacgac tgcgctcagt acccctgaat
      961 ccataacggc attgtcattt ttcctgagct gttccgctcg tcgctccgcg cgactacgtt
     1021 ctgtatcctc ctgctccttc cggcgcaatt cctcttcacg tttaagcaac tggactctga
     1081 gagctgcaag gccggatgcc ccccactgct gccactgttt ttcaaatgcc gtctgactcg
     1141 cgggaatgtc ctgtttctcc accttccggt aatgctgtcg caatttttcc atttgctggc
     1201 tcagcgactg gatttttccc tgttcgctac ggtattccgg tgccgcccat tgcatcaggg
     1261 gaatacggga aaaaccggac gaaaacggcg atgatttttt caggctggca gcatggctca
     1321 tcgcttcctt tcgttcggtg ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg
     1381 ccatcatcga catggcatta ctggtgagtt tccatgccgc attactgtcc ttcggcaacg
     1441 cttcgatgat gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcacg atattcagaa
     1501 ggtcagccac acggcgggca ggctctgtca gcccgctgtt cctgagttca gtgcgggaaa
     1561 tctgtatttc caggttaagc ctgttaatca gacgattctg ttcgccgcgt tctgtttcga
     1621 taccgcgtgt ttccatcgca caggcagact ttccgaggtg aataccaggc gttttctcaa
     1681 gcccctgtcg ctcgtatgaa cgatggtcta tctcttcctg ataaccggcg ttcgccagtg
     1741 ctctgtttgc atggtcagcc catgatgcac gccacgtctc ggcatgtgtc cggtcgttcc
     1801 agtccctgac ctttccgccg aacccagcag gtccgacagc cctcgtggtc agcatgatgt
     1861 gcgcatgggg atttgtttta tccatgtgat ggaacgccac atcagcaatc atgccctgac
     1921 tgacaaagtt ttctctgaca aaggcgagaa cggtttcccg tgcggcatcc tgcggcagtt
     1981 cacgggggat agcgagttca atttcccgcg caagttgtga gttcttccgg cattcggcct
     2041 tctcaacggc attccagagt tcggcacggt tcgtacacca ggcaggggct gtcgcggggg
     2101 tcagaatcac agcttcagcc acaccgcttt tgcgggtgta gtcgtgagtc agtccggtac
     2161 gctcatcaag catcagtgaa ccggaacgat aggcagcgga agctacggcg ctgtagccgc
     2221 tgcttctgga gataattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt
     2281 tttgtagttt tctttcagcc ccgcagggcg cacacaatcg aaacggagtg gagattgtat
     2341 aagtgcgcac tttgtgattt gtcgcgcatt atgccagctc agtttttaaa ctaccagtga
     2401 cgcgctaaaa tgcgattttt catcgcgcac ggagttacat catgagcaga acgctcgaac
     2461 agaagattgc tgatgctgag gcgagattac agcgcctgaa ggcgaagagc cggagcctgg
     2521 acacagcgca gaaggttgtt gtgggtgcag cattgctggc aaaggtcaga aagccggagg
     2581 aggtgcagtt gcgtgcctgg ttactccagt tcctgaaggc agaggtgaca cgacaggctg
     2641 atgtgacgcg catactgccg ctgattaacg agctggaggc gctgccagga cagtgattgt
     2701 atcgtccgct cgccgcagcc ccagcgaccg agcgttagcg agcgagtggg cgaggaagcg
     2761 gaagtgaatc cgtcgccagg ttgagcacat acgcacggtt tttcggtagg tgatatgcgc
     2821 tgaacccgca tgaatactgg atttccacag ggttggaagg ggagtattcg gaacttgcaa
     2881 ttctgctttc ttgcaacaca ttgtttttat tggttttttg ttttcagcgc cccaagttat
     2941 ccacaggctc gccgttaagt ttttagatct tgtttttaag atcttgtttt taatacttgt
     3001 tttagggtca aaattcatta ataaaatcaa tatgttatag aggtatatgt ggaggaattt
     3061 aaggtaaagg tggaggaatt taaggtgttt aggtggagga atttaaggta aaggtggagg
     3121 aatttaaggt gttattcaca gaaatgtgag gatataaaaa aaaatattcc acatttctta
     3181 tttgtggggc acattaaagc atcaccacat ttgaacggga tctttgtatg tcggacttga
     3241 ttgcctacaa gtcgaatgct ttggttgagg ccagctacaa gttgacccta caggaacaac
     3301 gttttctgtt gctgtgtatc agtcgattga aatctggtgc tgatgctgaa ttgcaaaaga
     3361 caatgaccat taccgctgcg gaatattttg attcattccc tgacatgggg cggaaaaatg
     3421 ccgaggttca gttacaggaa gcgatcgatc gtttatggga caggtcgatc attctcaaga
     3481 atgacgagaa gcgtgaggag ttccgctgga tccagtatcg ggcacagtac gctaaaggtg
     3541 aggccaaggc tcgaataacc ttttctgacg cggtaatgcc ttatctgacg caactgaagg
     3601 ggcaattcac gcgcgttgtt ataaaaaaca tatcaggttt atcaagtagt tacagtataa
     3661 gaatatacga gttcctccag caattccgca gtacgggaga gagaattatt gctcttaatg
     3721 actttcgttc aatgttaggg attgaaaata aatacaaaca attcagagac ttaaataaaa
     3781 tactaataaa accctgtgtt gacgaactga acaaaaaaag cgacctggct gtaacggttg
     3841 agacgatcaa aaaggggcgc acggttgtag ccctgcattt tcgcttcaag gaagacaaac
     3901 agatcaagat gacgatctaa aatcgatttt aaggcaccta aaacgctccc tgctgcgttt
     3961 tttttgtgtt agtagtgcga aggtactatt gaagtgaaaa attgcgtcaa aagcgctcta
     4021 atgcagcgaa aaaagaaccc ccaatttttg ggggcggaaa aacaaaacta tg
//
