LOCUS       CP056602                4072 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00322 plasmid pRHBSTW-00322_5,
            complete sequence.
ACCESSION   CP056602
VERSION     CP056602.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148577
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00322
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.8387109543322x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:13:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,156
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,867
            CDSs (with protein)               :: 4,867
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 60 of 171
            Pseudo Genes (incomplete)         :: 104 of 171
            Pseudo Genes (internal stop)      :: 30 of 171
            Pseudo Genes (multiple problems)  :: 19 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00322"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00322_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            54..308
                     /locus_tag="HV160_25765"
     CDS             54..308
                     /locus_tag="HV160_25765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLU64680.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            840..1532
                     /locus_tag="HV160_25770"
     CDS             840..1532
                     /locus_tag="HV160_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLU64681.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1788..3947)
                     /locus_tag="HV160_25775"
     CDS             complement(1788..3947)
                     /locus_tag="HV160_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLU64682.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     Typing          complement(3590..3948)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3949..4072
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.6300000000000002e-58"
     Typing          841..1532
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1792..4034)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aactaccagt gacgcgctaa aatgcgattt ttcatcgcgc acggagttac atcatgagca
       61 gaacgctcga acagaagatt gctgatgctg aggcgagatt acagcgcctg aaggcgaaga
      121 gccggagcct ggacacagcg cagaaggttg ttgtgggtgc agcattgctg gcaaaggtca
      181 gaaagccgga ggaggtgcag ttgcgtgcct ggttactcca gttcctgaag gcagaggtga
      241 cacgacaggc tgatgtgacg cgcatactgc cgctgattaa cgagctggag gcgctgccag
      301 gacagtgatt gtatcgtccg ctcgccgcag ccccagcgac cgagcgttag cgagcgagtg
      361 ggcgaggaag cggaagtgaa tccgtcgcca ggttgagcac atacgcacgg tttttcggta
      421 ggtgatatgc gctgaacccg catgaatact ggatttccac agggttggaa ggggagtatt
      481 cggaacttgc aattctgctt tcttgcaaca cattgttttt attggttttt tgttttcagc
      541 gccccaagtt atccacaggc tcgccgttaa gtttttagat cttgttttta agatcttgtt
      601 tttaatactt gttttagggt caaaattcat taataaaatc aatatgttat agaggtatat
      661 gtggaggaat ttaaggtaaa ggtggaggaa tttaaggtgt ttaggtggag gaatttaagg
      721 taaaggtgga ggaatttaag gtgttattca cagaaatgtg aggatataaa aaaaaatatt
      781 ccacatttct tatttgtggg gcacattaaa gcatcaccac atttgaacgg gatctttgta
      841 tgtcggactt gattgcctac aagtcgaatg ctttggttga ggccagctac aagttgaccc
      901 tacaggaaca acgttttctg ttgctgtgta tcagtcgatt gaaatctggt gctgatgctg
      961 aattgcaaaa gacaatgacc attaccgctg cggaatattt tgattcattc cctgacatgg
     1021 ggcggaaaaa tgccgaggtt cagttacagg aagcgatcga tcgtttatgg gacaggtcga
     1081 tcattctcaa gaatgacgag aagcgtgagg agttccgctg gatccagtat cgggcacagt
     1141 acgctaaagg tgaggccaag gctcgaataa ccttttctga cgcggtaatg ccttatctga
     1201 cgcaactgaa ggggcaattc acgcgcgttg ttataaaaaa catatcaggt ttatcaagta
     1261 gttacagtat aagaatatac gagttcctcc agcaattccg cagtacggga gagagaatta
     1321 ttgctcttaa tgactttcgt tcaatgttag ggattgaaaa taaatacaaa caattcagag
     1381 acttaaataa aatactaata aaaccctgtg ttgacgaact gaacaaaaaa agcgacctgg
     1441 ctgtaacggt tgagacgatc aaaaaggggc gcacggttgt agccctgcat tttcgcttca
     1501 aggaagacaa acagatcaag atgacgatct aaaatcgatt ttaaggcacc taaaacgctc
     1561 cctgctgcgt tttttttgtg ttagtagtgc gaaggtacta ttgaagtgaa aaattgcgtc
     1621 aaaagcgctc taatgcagcg aaaaaagaac ccccaatttt tgggggcgga aaaacaaaac
     1681 tatgcttgca ttatgtgtgt gcttcttgca ttcatccctg tcgatgaaca tagaagaaca
     1741 tcgacgaaca taacacgatg ttaaacaaaa caaaatattg ttcacattca ctgtgaccaa
     1801 ccgcgatcat gtctcctctc ggtggtgtgt gttcttgcat cttcctcgcg ccgccattca
     1861 tcccaggtac gattgaaacc cagagagcgg aacagatgat ccggctgttt ttctggtgtg
     1921 ttgacggcag caaaaagcgg tgcctcacgg gttttcagat gaaactccat aagttccggc
     1981 gcaggtgata tgtggctgtc tgcatcgtga tttacggcaa caccataccc tgcataccgt
     2041 aaccccttat cggtcagtgc actgatgacc agctggctgt tatcggctga gggaacggca
     2101 aacgcgggat acagcgtacc attaagccgg acaacaccgc ccagagagtc atccacgccc
     2161 atgaccagca aaagctgaag ctggcgggtt tcccggtcga cagaactcat taccggcatc
     2221 agcgtcggta gcagacgtcc ctgcgtaaaa tccaccttcc ttgctgccag tgatggcatg
     2281 gctggctggc ggtcttctcc aagacagaaa gactccggcc atgtcttcgg ctggcggaga
     2341 ttgcgggtat attgcggcca tgtctggcaa acctgcgtca gaagttgctc aaaggtcgca
     2401 gaggaataaa cacgttgcag tggtggatgt tgaccgattt cgcttcggtc acagatataa
     2461 ccactcctgt cacgcattaa atcaacacgc tcaaagattc tggtgcggta tttctccagc
     2521 tcgttccccc agagcgtgta ttctccgttc cggttgtgaa gcagaaggta acaggtcatt
     2581 tccccgttac caggcatggg ggcctcgcca taccccgtaa cgactgcgct cagtacccct
     2641 gaatccataa cggcattgtc atttttcctg agctgttccg ctcgtcgctc cgcgcgacta
     2701 cgttctgtat cctcctgctc cttccggcgc aattcctctt cacgtttaag caactggact
     2761 ctgagagctg caaggccgga tgccccccac tgctgccact gtttttcaaa tgccgtctga
     2821 ctcgcgggaa tgtcctgttt ctccaccttc cggtaatgct gtcgcaattt ttccatttgc
     2881 tggctcagcg actggatttt tccctgttcg ctacggtatt ccggtgccgc ccattgcatc
     2941 aggggaatac gggaaaaacc ggacgaaaac ggcgatgatt ttttcaggct ggcagcatgg
     3001 ctcatcgctt cctttcgttc ggtgttcagc tcttcccagc gtctgcgcgt cgcctgcata
     3061 tctgccatca tcgacatggc attactggtg agtttccatg ccgcattact gtccttcggc
     3121 aacgcttcga tgatgtcacg tagtgtgtac gaagttgcgt tgtcaggaag cacgatattc
     3181 agaaggtcag ccacacggcg ggcaggctct gtcagcccgc tgttcctgag ttcagtgcgg
     3241 gaaatctgta tttccaggtt aagcctgtta atcagacgat tctgttcgcc gcgttctgtt
     3301 tcgataccgc gtgtttccat cgcacaggca gactttccga ggtgaatacc aggcgttttc
     3361 tcaagcccct gtcgctcgta tgaacgatgg tctatctctt cctgataacc ggcgttcgcc
     3421 agtgctctgt ttgcatggtc agcccatgat gcacgccacg tctcggcatg tgtccggtcg
     3481 ttccagtccc tgacctttcc gccgaaccca gcaggtccga cagccctcgt ggtcagcatg
     3541 atgtgcgcat ggggatttgt tttatccatg tgatggaacg ccacatcagc aatcatgccc
     3601 tgactgacaa agttttctct gacaaaggcg agaacggttt cccgtgcggc atcctgcggc
     3661 agttcacggg ggatagcgag ttcaatttcc cgcgcaagtt gtgagttctt ccggcattcg
     3721 gccttctcaa cggcattcca gagttcggca cggttcgtac accaggcagg ggctgtcgcg
     3781 ggggtcagaa tcacagcttc agccacaccg cttttgcggg tgtagtcgtg agtcagtccg
     3841 gtacgctcat caagcatcag tgaaccggaa cgataggcag cggaagctac ggcgctgtag
     3901 ccgctgcttc tggagataat tttcatgctg agatgataaa tcgccatttc tgccgtaatg
     3961 cctttttgta gttttctttc agccccgcag ggcgcacaca atcgaaacgg agtggagatt
     4021 gtataagtgc gcactttgtg atttgtcgcg cattatgcca gctcagtttt ta
//
