LOCUS       CP056601                7939 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00322 plasmid pRHBSTW-00322_4,
            complete sequence.
ACCESSION   CP056601
VERSION     CP056601.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148577
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00322
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.8387109543322x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:13:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,156
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,867
            CDSs (with protein)               :: 4,867
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 60 of 171
            Pseudo Genes (incomplete)         :: 104 of 171
            Pseudo Genes (internal stop)      :: 30 of 171
            Pseudo Genes (multiple problems)  :: 19 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00322"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00322_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(7325..7939,1..978))
                     /locus_tag="HV160_25715"
     CDS             complement(join(7325..7939,1..978))
                     /locus_tag="HV160_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="QLU64678.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(1017..1715)
                     /locus_tag="HV160_25720"
     CDS             complement(1017..1715)
                     /locus_tag="HV160_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU64671.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(3080..3271)
                     /locus_tag="HV160_25725"
     CDS             complement(3080..3271)
                     /locus_tag="HV160_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLU64672.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3336..3659
                     /locus_tag="HV160_25730"
     CDS             3336..3659
                     /locus_tag="HV160_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLU64679.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3649..4359
                     /locus_tag="HV160_25735"
                     /pseudo=""
     CDS             3649..4359
                     /locus_tag="HV160_25735"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4337..4855
                     /locus_tag="HV160_25740"
     CDS             4337..4855
                     /locus_tag="HV160_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLU64673.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            4862..5095
                     /gene="mbeD"
                     /locus_tag="HV160_25745"
     CDS             4862..5095
                     /gene="mbeD"
                     /locus_tag="HV160_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLU64674.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="mbeD"
     gene            5806..6045
                     /locus_tag="HV160_25750"
     CDS             5806..6045
                     /locus_tag="HV160_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="QLU64675.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            6042..6404
                     /locus_tag="HV160_25755"
     CDS             6042..6404
                     /locus_tag="HV160_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="QLU64676.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(6429..7322)
                     /locus_tag="HV160_25760"
     CDS             complement(6429..7322)
                     /locus_tag="HV160_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="QLU64677.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin-like domain-containing protein"
     Typing          2925..3004
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(2350..2892)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          3304..3653
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          3650..5199
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagatattgc ttgtctgtgt aatttgtttt ttttatgtcg tttatttcgg cacagtataa
       61 atcaatttct gttcttgcca tttttatttg tctaaataga taaccaatgc gatttaatgg
      121 gagaaaaact tggaataaaa aagtggcaag cataataata ctaccacggc ttgctctacc
      181 atgaagaaac atccagtatg ctgataggat aatcagacta ctaaaaatga ctgaaagaaa
      241 agcggagctg ataccaaaca ggaaaaattc attatttact tttctttgtt tttctgaatg
      301 tgtatttaca gcttgaatta ctcgtttacg acttttatcc gtggtatgga aagctttatc
      361 cataggatac atgtgcatca tatcaaacat tcttccagat gcgaatttgg agctaagtgc
      421 aactgatttc atcattggaa atctacgttg tgtcattttt atgacaaata ttatatagat
      481 tgccattatt attataaact caatggctat taatgtatta acacttatac aaattgttat
      541 gattattcca atcatttgta ttaataatgg tactacatca gaacatattg tataaaataa
      601 tgatgatgca gaagaaagca tgctttctat aaccttattt agttctcctt tatttttctt
      661 taaaaaatgc tcttggtgag aattttgtat attatcaata acttgctctc taagctttac
      721 agataattct atatttgttt tttgagtcaa gtattccgat atgctatatc cagcaggcat
      781 aaggaatcta attgataaga aacatgctat aaatattaat aatgagttta tttctcctat
      841 tgaatcttta ttgtcaataa ctgtttttat taaatatccg aatagaacga aaagaaaact
      901 acttatagtt gataataata taataaaaaa caaattacat ttattaaatg tgatgatgta
      961 cttttcaata aaattcaatt tataatcctt aaagcccgta tattacgggc tttatgttat
     1021 ctagaattac atgagcaccc agcgccacat cctttaccgc tgcaaccaca aacagaacca
     1081 ttatcattcc aaacatcttt tcgcattatt atttttacaa attctgcggc tggaaggtga
     1141 gtgatactct ttactttttc gatgtaagaa tcactggata tggattcttt tggaattctt
     1201 atagtattaa tgttctttat gctgtatctt acagcataat caccatacca ctttgcgaca
     1261 gtttcatgac ctaggtaaaa agcgattttt tcaatttgat ttttatattc gttaaaacga
     1321 tattcttgga atgttatacc atgctcatta acatgcctta tctcattttt tagcatgtca
     1381 tttgtatttg gataatggtg gaggtaagca ccaagatatt tttcacaaaa gctggtataa
     1441 gcatctgtaa attgaataaa cgtatgccac atttcatcta taatttgtaa ttgctcgtga
     1501 acttttatat tgtagcattc cgttaggcat gttgaggcta gccagatgaa tttctttgtt
     1561 tcattaaaga tatcatcagc ttcttctggt gttacattcc attctttacg aaattttaga
     1621 agaacatcat cattgtgata actcatgata tcttctaatg agctaacatt atgaactttt
     1681 gcataagaac tgctgttcat cttatgttgt agcataacac ccccaattat atatttgaat
     1741 gtagatgaga atagttatca tttgttatgg tattgttaaa tttgtgcttg tgtcaatatg
     1801 cctaattcta taaatgagat agtaatcaag ttttttaatg gttataggat ccattagtaa
     1861 tacaaggttt ttgcagccag tttttacggg taggggctgc aaggaaaaca aaattttccc
     1921 actctaagag aagagaattg agttggttga atgagtggat gcatctaggg ccgctaattc
     1981 accgagtgta aaggctgtac gaaatgtgtt gcagtccgat aatagccccc tccattgacg
     2041 ttttacacat gtgttgtagg ttccaggagt gggcggatct caaaaagatc aaaggatctt
     2101 cttgagatcc tttttttctg cgcgtaatct gctgccagta aacgaaaaaa ccgcctgggg
     2161 aggcggttta gtcgaaggtt aagtcagttg gggactgctt aaccgggtaa ctggcttggt
     2221 ggagcacaga taccaaatac tgtccttcta gtgtagccgc agttaggcca ccacttcaag
     2281 aactcttaat atctcaatcc accttgtcca gttaccagtg gctgctgcca gtggcgcttt
     2341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgagccgag
     2461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2641 atgatgctcg tcaggggggc ggagcctatg gaaaaacgcc aacggcgcag ccttttcctg
     2701 gttctcgttt tttgctcaca tgtttctttt ggcgttatcc cctgattctg tggataaccg
     2761 catctccgct tttgagtgag cagacaccgc tcgccgcagc cgaacgaccg agtgtagcga
     2821 gtcagtgagc gaggaagcgg aagagcgccg gaacgtgcat tttctcctta cgcatctgtg
     2881 cggcatttca catcggacat ggtgcgcttt ccatacaatt cgtactgatg ccgcatagtt
     2941 aagccagtat acactccgct atcgctacgt gactggttca gggcttcgcc ccgaaacccc
     3001 ctgacgcgcc ctgaggggct tgtctgctcc cggcatccgc tcacagacaa gctgttaccg
     3061 tctccgggag ctgtatgtgt cagaggtttt caccgtcatc cccgaagcgt gcgaggcagc
     3121 tgcggtaaag ctcgtcggcg tggtcgtgaa gtgattcaca aatatcggcc tgttcatctg
     3181 cgtccagttc gttgagtttc tccagcagcg ttaatgtctg gcttctgata aaacgggcca
     3241 tgttaagggc ggttttttcc tgtttagtca ctgatgcctc cgtgtaaggg ggatttctgt
     3301 tttatggggt aatgataccg atgaaacgcg agaggatgct caccatacgg gttactgatg
     3361 atgaacatgc ccggttactg gaacgctgtg agggtaaaca gctggcggta tggatgcggc
     3421 gggtctgcct gggtgagccg gttgcacgct ccgggaagct gccgacactg gcaccgccgt
     3481 tactgcgtca gctggccgcc atcgggaata acctgaatca gacagcgagg aaggtgaaca
     3541 gcgggcagtg gtcttccggt gatcgggttc aggtgttggc tgcgctgatg gccatcgggg
     3601 aggagctgcg tcggctgcgt ctggccgtca gggaacaggg cgcgcgggat gattgttaaa
     3661 tttcatgcca ggggaaaagg cggtggcagt ggtccggttg attacctgct gggaagggag
     3721 cgaaaccggg aaggggcaac ggtgcttcag ggtaatccgg aagaagtccg ggaactcatc
     3781 gatgccacgc catttgcgaa gaaatacacg tcaggtgttc tgtcgttcgc ggagaaggag
     3841 ctgccgccgg gaggacgtga aaaggtgatg gcgagctttg agcgtgtact gatgcccggt
     3901 ctcgaaaaga atcagtacag catcctgtgg gtggagcacc aggacaaggg acggcttgag
     3961 ctgaattttg tcattccgaa catggagcta cagaccggaa aacgcctcca gccgtactac
     4021 gaccgggcag acaggccgag aattgatgcc tggcagacgc tggtgaatca ccattacggg
     4081 ctgcatgacc ccaacgcccc ggagaaccgc cggatactga ctctccctga taacctgccg
     4141 gaaacgaaac aggcgcttgc agagagcgtc acacgcggga tagacgctct ttatcatgtc
     4201 ggggagataa aaggtcgtca ggatgtgatt caggcgctca cagaggcagg gcttgaagtg
     4261 gtcagggtga tgcgaagcag tatcagcatt gcagacccga acggtgggaa gaatatcagg
     4321 ctgaaaggag cattttatga gcaatctttt acagacgggc gcggagttcg agaaaaagct
     4381 gaaagagaga gccgaatcta cagagacaat gctgaacgac gagttcagca agctcgaaaa
     4441 atctgtaagc aaggctgtga catcaaacga gacgaaaatc aaagacgcta tagccctgtt
     4501 cacagcttcg acagaggaat cactgaaaaa acaccgggaa ggggtgaaag aggcgatgat
     4561 gcagcacagg aaggacgtgt taaagctggc agggaatacg ggcatgatgt tactggggat
     4621 ggtccttttc ctgtttaccg tgagtggcgg gacgctctgg tatcttggcg ggatgataca
     4681 ggcgaacctg gaagaaatca ggatacagga agaaacattg cagaaactga acgcgaagac
     4741 atggggcgtg gagtttgtgc aggacgggag aaggaaattt ctggttatcc ctcaggggaa
     4801 atcggcaacg gtgattccct atcaggggaa ggactgggta caactgacgg agtgacacag
     4861 agtgacggag ctggaaagac atttgctgaa cgccttagag caactgcaac aggactatat
     4921 gcagcggctg aacgaatggg agagcgcctt cgtggaattg cagaaaatgt tttcgcttac
     4981 gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca
     5041 cttgagcgag cagacagaac gcttgagcca gttatacagc gagaacagga gataagggat
     5101 gaacggctga tacaggaacg cgaacatgag ttatccctgg agcgtgagcg ccagctggaa
     5161 atacaggaac ggacacagga tggcccttcg ctgggatggt aatcagagac aggctaaaat
     5221 gttgtcagcg cgtttccggt gcattttttg ggataatggt tggttcgggg gcaccgggaa
     5281 cgcgcttcag tcagaccttg ctgaaaacac aggaacgcgt taaaacgcat acgggcgcga
     5341 ttctggtgtg tcttttgttg ctgtgttttg cctctattgc cagatcgcgc tcattcccct
     5401 gaacttctgt tttcccgctc cggtccccgt cttcttcgct tccgaaagca tgaaaaaaat
     5461 ccgtttcagc tgtctctggc tggcaattcc tgcgtgattc acatggctgc atagctatgc
     5521 atgtggttaa aatttaccgg gtgcgatcgc ggcagttttt cgggtggttt gttgccattt
     5581 ttacctgtct gctgccgtga tcgcgccgaa cgcgtttcag cggtgcgtac aataagggat
     5641 tatggtaaat tatcttgttc cttagagaat gatttgaggt ggtatcctgc acttatttaa
     5701 atatgcttgc ttgggaaatt ccaggttgca gtatggttga gttgtaattt tcctatgata
     5761 ggaaaaatgt tatattatta atttatttgt gaggcttcat aaagaatgaa aatttctgat
     5821 attgaaaaaa acatatataa aggactagct gaatttattt cacaaaaaat cagagatgaa
     5881 catgatatat gccttggaca gtttgaatca gaggaattac ttgatgagat tcttaaaaga
     5941 gtaatcccag tcatatataa tgtggctatt gatgatgcta taaaatcagt aaggaatgtt
     6001 ggggatagat tagaggagga attagatatg aggaaggtag tgtgactaat tttaaatcag
     6061 acttaaatct aataagaaaa gaatggtata atgcatttta ttcaggtaat actgaaatgc
     6121 tagattactt ggaagctgat tggtttattt caacaaatgg tcaaaaagtt atctataaaa
     6181 aacatcagtt acgaaagatt gatatgcgtc cagcgaggat tttacctagc atagaaagat
     6241 atgaatatga tgtcgtaatg cgtgagttta aaggcattgc ttgtgtttca gggaaagctg
     6301 aaataaagca aaatgatgga aatatattaa tggcttttat tgagaattgg ataaaggtta
     6361 atggtggatg gaaaatgcag tttatatcgt atgaaaaaat ctaaggggaa gcccccttag
     6421 attaatggtt atatatgttc aagttttatt tttttatagc gataagttct gctttgagct
     6481 ttaagcatgt tttctgcaaa ctgaatatca aaactattta tagcattagt tattatagtt
     6541 tttatttttt ctttatcaga taatgaatca ccatgctcta taggttcccg catatatgga
     6601 ttgtttttta attcatcaaa taatgtttta aatagttcta atcgacgagg tcttataact
     6661 gtaaaagata tatgaagaga gtgtttgctc tgagtcgtaa cattgtgcca gtatccagca
     6721 ggaagatata tggcctgacc tggggttata tcaaactcat aatctgggga ggtattaggt
     6781 ggtagataat cgaagcttct ttcatcttcg agagggtatg aataggtagg gttgtaaact
     6841 ttccattttt ttattccctc aatttgaaca gctattacat catgattgtc aaaatgaagc
     6901 ccaaagcttg gtgttgctgt aaatgcagca tatagattag cactacatgt acattctaac
     6961 tctttagata accatagtct gctttcatct acagattcaa agaatgactg gcatcgatct
     7021 actataaggg ttgcgccatc acgtaagcat ttatatagct gatggcgatt tatatgtggt
     7081 gttctatcgc ctgtttggct atatgtgtat cttataaatc ctttataccc cctaatttcg
     7141 ggaaagttgt catttgccat tctaactcta ggatagtgca atatatcttt ttcaagcaat
     7201 ttattaagat cattccagtt gattaagttt ttataattaa catccgtgaa tacaaacgga
     7261 tatttctgga aattatattt cttaaatttt tctatgattt ccttttttat aagattattc
     7321 atttttaaac ctcatgtttt gtgatatcta taatctgtgc tttaggtata ttatttataa
     7381 gttgttcatc atgagatata acaatgatat gcttttcctt gttttcctct tttatataat
     7441 cactgattat ttttatacca agtgaatcaa acccttttgt cggttcatcg aaaataatca
     7501 agtctacgtt ttttccagat gtaagagata ctcctaattt ttgtttttgt ccaccagata
     7561 aattatgtcc gtaacctttg atctcataat ctagtggttt gtcgaactta aatctgtgta
     7621 gcagcttttt aattgtattt aaattattaa tgccggaata atgtaatata ttttcctcta
     7681 tagttccagg catgagatct agatcttgag gaacgtataa gacattaaca aaaggagaat
     7741 ttgctttttc ttgttgtatc ccattgattt ttattgtgtt ttcttctgtt gttatattgc
     7801 cagagagaat ttttgcaata gttgtttttc ccgaaccatt ctcaccggtt ataaaggtaa
     7861 caacgccttt tgttaatatt aatttatggt tgaatgattt gttgtatatt tcaatattgc
     7921 aaatattgtc ttttacatg
//
