LOCUS       CP056563                2507 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00399 plasmid pRHBSTW-00399_4,
            complete sequence.
ACCESSION   CP056563
VERSION     CP056563.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148601
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00399
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107.045699158051x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 02:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,160
            CDSs (total)                      :: 5,044
            Genes (coding)                    :: 4,890
            CDSs (with protein)               :: 4,890
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 55 of 154
            Pseudo Genes (incomplete)         :: 84 of 154
            Pseudo Genes (internal stop)      :: 26 of 154
            Pseudo Genes (multiple problems)  :: 10 of 154
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00399"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00399_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(83..286)
                     /locus_tag="HV184_25790"
     CDS             complement(83..286)
                     /locus_tag="HV184_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLW24153.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGGDAGAMMLDAYRQILSET
                     EPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            1521..1748
                     /locus_tag="HV184_25795"
     CDS             1521..1748
                     /locus_tag="HV184_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QLW24154.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2114..2299
                     /locus_tag="HV184_25800"
     CDS             2114..2299
                     /locus_tag="HV184_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLW24155.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2347..2434)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          578..1106
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 cgtccataac agcatcatgg aacgacgacc accgtccgtg acggccgcct cgtttaagta
       61 tggacagaaa tacagaaaat gctcaggacg aaatgtaatg aatgcgaacg gattcaagaa
      121 attcgagcat gacagtcctt acggccggtt cggtttcaga caaaatctgc cggtatgcat
      181 ccagcatcat ggctccggca tcccctccgg catcccctcc ggcacgccgt agccagaccg
      241 aaacaacgga cacaagcagg tgtcgctcat catcactaag agtcatcagg gctccggaag
      301 aaaaaccaaa catggcttac ctactgttaa cggcaacgca gaagaaaggt ttacagggta
      361 gcgggcaggg atgaaaaaaa aaaaattgca gtcaggccgg aacggctgct gaaccggtac
      421 aggcgggttc cgggcgcagc gccggaccag tcacggagca ctagcggagt gtatactggc
      481 ttaagatgta gcatggcagt aacacatagc attaatgcca cataagacag gcgcagaaag
      541 tgaaagaaaa cgcgtcagga gaaaatgtga tacagaatat attccgcttc ctcgctcact
      601 gactcgctcc gctcggtcgt tcggctgcgg cgagcgggac gggcttacgg atggatcaga
      661 aatttcctgg gggatgccag gaagatactt aacgaagaaa gcataaggcc gcgccaaagc
      721 cgtttttcca taggctccgc ccccctgaca agcatcacag aatctgacgc tcaaatcagt
      781 ggtggcgaaa cccgatgcgg cttaaagata ccaggcgttt ccccctggtg gctccctcgt
      841 gctcccccgc attcctgcct ttcggtttac cggtgtcact ccgctgttat ggccgcggtt
      901 tctcattcca cgcctgacac tcagttccgg gaaggcagtt cgctccagga tggactgtat
      961 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc aacttgagtc
     1021 caacccggaa agacacgact aatcgccatt ggcaacggcc attggcaaac aggatgtgcg
     1081 agagatgtct caagagttct tgaagtggcg gccttagtga ggctacactg aaaggacaaa
     1141 tttggtgaca tgtctcgcac aagactgtta ccacggttag gggatctgcc aggattacct
     1201 aaccttcgaa aaaccacctg ccagggtggt tttttcgttt acagggcaag agattacgcg
     1261 cggaaaaaaa ggatctcaag aagatccttt ataaattaga catccaacct cacaaagagg
     1321 aatacttaaa tcatcttggt atatgtatcg gccggattac atctcatata agcctcctcc
     1381 ccgcctgaca ccacactttg accatagcac ctcgcgccta actcgatata cagcccacca
     1441 tcaagcctct tttcacataa aaaatgacct atagttagat ggttagtgac caaaactgat
     1501 atggataaaa ggagctggct ttgaaaaaga tttttgtttc agtgttcgca gcggcagttg
     1561 cgctttcagc cctcaccggc tgcacgcgca caagctatgc catacacacc aatgacggtc
     1621 gcactattgt cagcgacggc aaacctaaag aatcagactc aggcctgctt ggctatacag
     1681 acgcgaatgg tgtcaaacag cagatcaata aaaccgacgt taaagaagtc tccgaaattc
     1741 cgcactgagc atcttctccg aaaaaggccg ctaatgtggc ctttttcttt acctgtggtt
     1801 ctccgccaaa atcccagcaa attgcatcac caaagctaaa agctttcagg gttgtctaaa
     1861 aagcacaaga cataagagga agtgcggtat tttataatca agcccccaag aatttaccat
     1921 aacatccgtt gcccgcaccg cctgagacgc gttcagcgcg ttcctgacga aaccatgaca
     1981 aaaaccacaa caaaccaccc cggaacccgt cagaaacgcg cctgttaaat tttaacggca
     2041 tgcatgacta tgcaccagaa tgacgccatg ctcttttcac gcaaaaatca tcaccagacg
     2101 gggaaaatca ccagtgacca gacaggaatc cgccgccctc aatatggcca aatttatccg
     2161 cgcacagaca cttctcctcc ttgagcggct cgagcagatg gatctggatg aggctgccgg
     2221 ctgctgtgag cacctgcacg atcaggccga agcgctttac gccatgctga acgcacagat
     2281 aggcgaggaa aatgcgtgaa aatcggtgaa cgggtgcgca attcagtgcg cggccgtgag
     2341 gcgatggcgg ggtgtcgggg cgcagccctg accagggtat ttgtgatgcc ggcgcgtgcg
     2401 cggtattaca aatgcacatc ctgtcccgga acggacaccg ggaaacagca aaaaaaaccg
     2461 ggcggcacgc ccggaactca atcaagttag attagattac tctcact
//
