LOCUS       CP056544               12988 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Klebsiella oxytoca strain RHBSTW-00432 plasmid pRHBSTW-00432_6,
            complete sequence.
ACCESSION   CP056544
VERSION     CP056544.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148609
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 12988)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00432
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.0098307145216x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:08:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,060
            CDSs (total)                      :: 5,942
            Genes (coding)                    :: 5,737
            CDSs (with protein)               :: 5,737
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 84 of 205
            Pseudo Genes (incomplete)         :: 122 of 205
            Pseudo Genes (internal stop)      :: 32 of 205
            Pseudo Genes (multiple problems)  :: 30 of 205
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00432"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:571"
                     /plasmid="pRHBSTW-00432_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(887..1018)
                     /locus_tag="HV192_30225"
                     /pseudo=""
     CDS             complement(887..1018)
                     /locus_tag="HV192_30225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /name="DNA-binding protein"
     gene            complement(1022..2169)
                     /locus_tag="HV192_30230"
     CDS             complement(join(1022..1932,1932..2169))
                     /locus_tag="HV192_30230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="QLU28099.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIKK
                     YGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNTCC
                     WPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLDL
                     RDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPDERWV
                     TDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPQKQVLV
                     HSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTRE
                     EARSDIFDYIEMFYNSKRRHGSSDHMSPTEYENQYYQRLGSV"
                     /name="IS3 family transposase"
     gene            complement(2233..2541)
                     /locus_tag="HV192_30235"
                     /pseudo=""
     CDS             complement(2233..2541)
                     /locus_tag="HV192_30235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2541..2732)
                     /locus_tag="HV192_30240"
     CDS             complement(2541..2732)
                     /locus_tag="HV192_30240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLU28100.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3203..3394)
                     /locus_tag="HV192_30245"
     CDS             complement(3203..3394)
                     /locus_tag="HV192_30245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLU28101.1"
                     /translation="MNKQQKTAVNMAKFIQAQSLLLLDRLNELDLAHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4317..5014)
                     /locus_tag="HV192_30250"
                     /pseudo=""
     CDS             complement(4317..5014)
                     /locus_tag="HV192_30250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(5252..6166)
                     /locus_tag="HV192_30255"
     CDS             complement(5252..6166)
                     /locus_tag="HV192_30255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002912403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QLU28108.1"
                     /translation="MLLSIIIPCYNCESNIEKLLLSIKEQLNETVQLVLINDGSWDATH
                     DIITKFTSDNPSIKIKYINSINQGAAKARSTGLNYAEGDYVFFCDSDDMISANFISVFT
                     ELSNDKPDMIYFCSTVLDSQGNRFKKVHFPLAYIESNSNEVFNYLLKKGQWTAAVWTYI
                     FRKDLVLKSGAIFTDRKVHEDHLFTLRLLAGSEKISICNSELYFQNVTSGSLTKSPKKI
                     DYLTERYKSYKEAREDIIYKFSQESIVLYDRWSMSSLLQILKENKKMVFQGIMHTAIYR
                     VLWKEKLVILDFVKSKVKKNHEI"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(6166..7632)
                     /locus_tag="HV192_30260"
     CDS             complement(6166..7632)
                     /locus_tag="HV192_30260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="QLU28102.1"
                     /translation="MNLKSKTIHAVKWNLLSTVCVTGLGMVSLWILSSLFTTTDYGIIS
                     AALILSTVFSIITDFGISNSVIRSEKLNKYELSSLYIINVFLGMIFCLGLFIFSAQLAS
                     LFNGGTELAKQIKIMSFSLIISSFGGQPRALLARELRFDVISRITILSAIFNFVFAVFL
                     SYVYRSPWCVAVSLLVSSSISSVLYIYSSKGLRDIKLQFFTLKEIKKHVSYGSQLVMDS
                     IINQISVNTYPVLMARLISLTAIGGYNISYNISVALFEKLNPVLSHALFPAFSKIQNDA
                     VSLKKTFLKVTMFSSLVNFPMLLGMMLISDYVVTVFFEQKWQFITPIVQILCLVGAARS
                     LDVPVISVLLAKGQMYRNVYLGVFKLALGIPLTWFLGKQYGLTGIVFSFLCIQIVNLVM
                     GYFFQVRPCLNIKVKSYIYAVFVPLIHTLPMLVTGYLLILFLTITSPILSMVVIIFSCM
                     SVYIASILTSPVKEIKEFNAIVLNNILRNK"
                     /name="MOP flippase family protein"
     gene            complement(7913..8740)
                     /locus_tag="HV192_30265"
     CDS             complement(7913..8740)
                     /locus_tag="HV192_30265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QLU28103.1"
                     /translation="MISVNLTTMHSRLDLCAATVWSIINQSVQPDKINLWISNEPYLSD
                     HGICEIPAWVEELNKINNIVNVHYVKNTGPYRKIINAIINSEIDDILVYADDDVIYGRQ
                     WLEFLLGQFYENNCNVAIAARIRIMERNIFNKYKSYSSYSISMEKDKFEKDFIITGVGG
                     CVLSKRMIKPSIIGDDAYLTISPKTDDIWISKMLEVSNTKVESSPDALRCVHEILHGYN
                     TLSSINTLTSKNSSLIIKAIRMVKNKFYSYFGLQKTNNDKSIKMVNLHFNGKL"
                     /name="glycosyltransferase family 2 protein"
     gene            8922..9606
                     /locus_tag="HV192_30270"
                     /pseudo=""
     CDS             8922..9606
                     /locus_tag="HV192_30270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(10088..10492)
                     /locus_tag="HV192_30275"
     CDS             complement(10088..10492)
                     /locus_tag="HV192_30275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="QLU28104.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRVFGAFAPVPVEQRGEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEMRQNTTRPRAWWRFWQS"
                     /name="DNA-binding protein"
     gene            complement(10492..10683)
                     /locus_tag="HV192_30280"
     CDS             complement(10492..10683)
                     /locus_tag="HV192_30280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLU28105.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            11095..11208
                     /locus_tag="HV192_30285"
     CDS             11095..11208
                     /locus_tag="HV192_30285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLU28106.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(11772..12892)
                     /locus_tag="HV192_30290"
     CDS             complement(join(11772..12634,12634..12892))
                     /locus_tag="HV192_30290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="QLU28107.1"
                     /translation="MKKRFSDQQIISILREAEAGVSARELCRKHAISDATFYTWRKKYG
                     GMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVVLMCDTT
                     GLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLRREGL
                     HVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALATGRRI
                     KCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTCRALDQ
                     WAYEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDVSHARKTISEWRQDYNE
                     CRPHSALNYQTPSEFAAGWRKGNSESEGSDITN"
                     /name="IS3 family transposase"
     Typing          3270..3896
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.783"
                     /coverage="99.0"
                     /evalue="7.089999999999999e-172"
     Typing          10732..11208
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.664"
                     /coverage="92.0"
                     /evalue="3.49e-179"
ORIGIN
        1 ttttgagtat ctttggctat acagtcctat cccttattac cggagtaatc atagggaatg
       61 taatcgttcg ggtgttatct acaacagaac tcggaatgtt gttcgcaaat ccattagcga
      121 tgatatgtag cattaccggg gtaatagtcg tagctgtaca atatttacca taacatccgt
      181 tgtccgcacc gcctgagagg ccctcagggc gtttctgacg gaaaaccggt aaaacaggca
      241 acaaaccacc cgaaaacccg ccagaaacgc gctggctctg tttttacggc atgcatgagt
      301 atgcgcgcaa gtgcatagcg cggaaattca cgccattcag ccggggtaag ccctgtttcc
      361 ggcgttcttc ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc
      421 gttgccggcg cagccggaaa accggttttc agcacgttca ggaatcgggt tcgtcgacgg
      481 ccggcaatca cgttatggca agtcgccgga cgactcaccg gcgattcgac tcgtgacttg
      541 actctagagt caccgcggtc aaaaaaacgc caaagcgacg attcgactct agagtcaagt
      601 cgtcataagt tactgatagt gtggagtgag tcgtcgatga ctctatgacg agtcgtcggc
      661 aagtcgtaca cgaatcaccg acgacttgga ttgaccttac agaaggtaat actgcgtaat
      721 ttaataacaa ccaactgact gatattaatg agttatctgc aaaaatttaa ttttgcgtgg
      781 ggtgtggata tttttagtgt gaggagcgtt cagagatctg gcgatttttg ggaaggttca
      841 cgaacgcccc ggcatggccg gagaggaaaa ctcaagcatg gcgggatcag gattttttcc
      901 agaatttcca ccacggggaa tggggtggtg aagaaggttc gtgcttgtgc tccagaagct
      961 gcatagcctg acgaagggaa tcaatatgct catctcgagc ctttgaatcg ccacggataa
     1021 tctagacact tccgagccgt tgataatact ggttttcata ttctgtcggt gacatatgat
     1081 cgctagaacc atgccgacgc ttactgttat aaaacatttc gatgtaatca aaaatatcac
     1141 tgcgggcttc ttcccgcgtt ccgtagatct ttttctttat ccgttcgcgt ttcaacaact
     1201 ggaaaaagct ttctgcaacc gcattatcat ggcagttacc gcgacggctc atgctgcctt
     1261 ccaggccgtg tgatttcagg aacgactgcc actcatggct tgtgtactga ctgccctgat
     1321 ccgaatgaac cagcacctgt ttttggggat tacgccgcca tacagccatc agcagtgcgt
     1381 tcaggacaat gtcctttgtc atccgggatt gcatggacca gccgataatt ttgcgtgaga
     1441 acagatcaac aaccacggca agatacagcc agccttcgtg ggtcctgatg taggttatgt
     1501 ccgttaccca acgctcatcc ggagcatccg gattgaactg tcgctggagc ctgttgggcg
     1561 acacgatact ggcctcgcct ttacgtgccc gcgggctccg gtatccgacc tgagccttta
     1621 tcccgacacg tttcatcagt cgccagaccc tgttcactcc gcactgttgc ccgctgtcac
     1681 gcagatccag atggattttg cgataaccat agacgcatcc cgattccagc cagaactgtt
     1741 taatctgtcc tgtcagtctc aggtctgcct gatggcgttg tgaatgcggc tgctgaagcc
     1801 aggcgtaaaa accactggga tgaacatcca gcacccgaca gagcaggcga acaggccagc
     1861 aacaggtgtt gtcacggata aaggcgtacc tcagtcggac agctttgcga agtacgccgc
     1921 ggcttttttt aatatgtccc gttcgtcggt aacccgcttc agctctttct ggagacggcg
     1981 gatctcggcc tgagcatctg actgttcttt atttgtggaa gaatccggac cgtacttctt
     2041 tatccaggcg taaaggctgt gggtggtgat atcgagacgt gttgcaacgc tggcaacaga
     2101 ataaccgcga tcaacaacct gtttgactgc ttcagtttta aactcttcgg gataacgctt
     2161 accgctcatg ggcacctctc tttaagccat cttaaatgac tctaaggtgt ctgttaaacc
     2221 cgtggcgatt cacttaacag cccctttaag accatcgttt tcagcttcaa gtaaagcgat
     2281 tctatgttgt aaatcatgta aaccaacgct gttaatacct gtagattctg cgtcactttt
     2341 tacatcatgt tttacagcat cgattacacc atcaagagaa ccaaacactc taataagctc
     2401 tgatgtatca ataactttaa tacccttccc gtgctttaca acagaggtgt aacttaactt
     2461 accactgtta atcatcttgt aaatagtagg gcgactaacc ttaacaagcc gtgctgcttc
     2521 actaatagac actctggcca tcagttacca tcctgtaatg catccagtct cgaggacagt
     2581 gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaaggtcggc ctcagcatcg
     2641 agatcaagct cgttaagctt ctccagcagg agcagagact gattctggat gaatttagcc
     2701 atgttcagcg ctgttttctg ttgtttcgtc ataagggacc tcagagcatc agtgctcagt
     2761 gtaaaaccgg taagggggtc ggatgtacga atggttcaga gaacggatct gaggtaatcc
     2821 tgtagttatt ttgtagttta tctgtagtca tcgcactaaa aatcaggatt aagtcatcag
     2881 cttttgtagt ctttctgtag tttacctgta gtccggaaaa ataatccgtt catgcgagca
     2941 tgaacgaggt cgttaatgac gtttttttgt caccgacgcc ccttaacctc cgcagatcac
     3001 ccggccagaa tgataatttt ttggccctta aatggtcata cacctaacgc agctgcgatt
     3061 ttcatcgcaa cttcatgaaa cataaataaa aaagaaaaaa tacagggatt ctgggtatta
     3121 ccggggtaat aatgttaagc gccaggacaa tgaaaattaa ggcgatgccg gtattactgc
     3181 ggtaataccc agaacggagc cattacgcac cgtcctgctg ctcattcagg cgcgctgaca
     3241 ggcttcggta aaggcgttcc gcatcctcat ggagtttctc gcagaaatca gcctcatgag
     3301 ccagatccag ctcattaagc ctgtccagaa gaagcaggga ctgagcctgg atgaatttag
     3361 ccatgttaac ggctgtcttt tgttgtttgt tcatgaaggc cccggcgcga aagtgcttac
     3421 tgtacgttct gatggttttc cgcttcctcg ctcattcgct cactccgttc ggtcatgagt
     3481 ctgcggcgag cggagggaag ataagcagat aacgtaacgg taagcataac gaaagctaag
     3541 gacaagggcg aaaaggccac gccccagcgg ggcgtttttc cataggctcc gcccccctga
     3601 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     3661 ataccaggcg tttccccctg atggctccct cgtgcgctct cctgttcctg cctttcggtt
     3721 taccggtgtc attccgctgt tatggccgcg tttatctcat tccacgcctg acactcggtt
     3781 ccgggaaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     3841 gctgcgcctt atccggtaac tatcgtcttg agtccaatcc ggaaagacac gacttatcgc
     3901 cactggcagc agccactggt aacaggattc acggagcaga gtagtagagt gcttctacag
     3961 agttcttgaa gtggtggcct gactgcggct acactagaag gacagttttg gtatctgcgc
     4021 tccgtgaaag ccagttacct tcggaaaaaa gagttggtag ctcttgattc cggcaaacga
     4081 accaccgttg gtagcggtgg tttttttgtt tgcaggacat gagattacga cgcaacagaa
     4141 aggatctcaa gaagttcctt tctgtattgc tactatccta gagatctgac ccattagggg
     4201 cattatcaga ttacggcctt gtaatccacg ataaaaaccc agaaaccaga agagttggcc
     4261 cagcgacata tgttacataa acgcccacat atgaaactgt aggtaatgac tccaacttac
     4321 tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc caccgatttt
     4381 gagaacgaca gtgacttcct gcccagcctt gccagatgct gcctcagatt caggttatgc
     4441 cgctcaatgc gctgagtgta acgcttgctg ataacgtgca gttctccctt caggcgtgat
     4501 tcataaagcg gccagccatc cgtcatccat atcaccacgt caaagggtga cagcaggctc
     4561 ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaat aaccgtcttc
     4621 cggagcctgt catacgcgta aaacagccag cgctgctgcg atttagcccc gacgtagccc
     4681 cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat gcgcgaggtt
     4741 acctactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca gcgcccataa
     4801 tgcgggcggt tgcccggcat ccaacgccat tcatcgccat atcaataatt ttctggtgcg
     4861 taccgggttg agaggcggtg tatgtgaact gtaactgcca tgttttacgg cagtgagagc
     4921 agagatagcg ctgatgtccg gcagtacttt taccgttacg caccaccccg tcagtagctg
     4981 aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata
     5041 cactaaatca gtaagttggc agcatcaccc agaaagacca taaaattagc ctataacggc
     5101 aaatagtaat gctgccaacc tgttggtgac catttgaatc tgtagccatt caatccttgt
     5161 aacatcaggt gatcctggcg tcagtgaaaa tgcgaaatca acaaattggc atcattaccc
     5221 attatacgat aatccctatt gggataggct cttagatctc gtgatttttt ttaactttgc
     5281 ttttaacaaa atctaaaatc actaattttt ccttccacaa tactcgatat atagctgtat
     5341 gcattatccc ctgaaacacc atttttttat tttcttttaa tatttgcagt agcgaagaca
     5401 tagaccatcg atcataaaga acaatgcttt cctgactgaa tttgtaaatg atatcttccc
     5461 tggcctcttt ataagactta taacgctcag ttaaataatc aattttttta ggggatttag
     5521 taagtgaacc tgaagtcaca ttctgaaagt aaagctcaga attacatatt gatatttttt
     5581 cactaccagc gagaagtctc agcgtgaata agtgatcttc atgtaccttt ctgtctgtaa
     5641 atatagcgcc agatttcaat acgagatctt tccggaaaat ataagtccat acagcagctg
     5701 tccactgccc tttcttcaaa agataattaa aaacttcgtt actattgctt tcgatgtatg
     5761 ctaatggaaa atgtaccttt ttaaacctgt ttccttgcga atctaatacc gtagagcaaa
     5821 aatatatcat atccggttta tcattactta actctgtaaa aaccgatatg aaattagcag
     5881 aaatcatatc atctgaatca cagaagaata cataatctcc ctctgcgtaa tttaagcccg
     5941 ttgatctggc ttttgccgcc ccctgattaa tactattaat gtattttatt tttatagagg
     6001 ggttgtcact tgtaaattta gttattatgt catgcgttgc gtcccatgag ccatcattaa
     6061 tcaacactag ttgtacggtt tcattaagct gctcttttat tgagagaagc aatttttcga
     6121 tatttgactc acagttataa catggtatga ttattgataa taacatcatt tattcctcag
     6181 tatattgttt agaacgattg cgttaaattc ctttatctct tttactggag aggtgagtat
     6241 agaagcaatg tatacagaca tacatgaaaa aataatcaca accattgaca aaataggaga
     6301 ggtaatagtt aaaaaaagaa tcaacaggta acccgttaca agcataggta aagtgtgtat
     6361 aagcggtacg aagacggcat agatgtaact ctttactttg atatttaaac agggtctaac
     6421 ctggaaaaaa tatcccataa caagatttac tatttgaata cataagaatg aaaacactat
     6481 ccctgtaaga ccatattgtt tacccagaaa ccatgttaaa ggaataccaa gcgctaactt
     6541 aaatactccc aggtaaacat ttctgtacat ttgcccttta gcaagcaaga cagagataac
     6601 aggcacatca agggatcgcg ccgccccaac caaacataaa atctgtacaa taggggtgat
     6661 gaactgccat ttctgctcaa aaaaaacagt cactacatag tctgaaatta acatcattcc
     6721 caataacatc gggaaattca ccaaagacga aaacatagtt acttttagaa aagttttttt
     6781 cagagatact gcatcatttt gtatcttgga aaaagctgga aataacgcat gtgataaaac
     6841 aggattcaat ttttcaaaaa gcgccacgct aatgttgtag gatatattat atccaccgat
     6901 agcagtcaaa cttattaagc gtgccattag tacaggatat gtattcacgc ttatctgatt
     6961 aatgatagag tccatgacca actgagaacc gtagctgaca tgttttttta tttccttcaa
     7021 agtaaagaac tgtaacttaa tatctctcaa gcctttgctt gaatatatat acaatacgct
     7081 actaatagat gaggaaacca acagtgaaac ggcaacacac cagggggaac gatagacgta
     7141 gcttaagaag actgcgaaca caaaattaaa aatagctgac aggattgtta tcctggatat
     7201 tacatcaaat ctaagttctc tggctaacaa agctcttggc tgtccaccga aagacgatat
     7261 tataagagaa aatgacatga tttttatctg ttttgccagt tctgttccac cattgaataa
     7321 ggatgctaac tgagcactaa aaataaacaa gccgagacaa aatatcatac ctaaaaagac
     7381 atttattata tacagtgaag acagttcata tttatttaac ttttcagatc ttataactga
     7441 gtttgatata ccaaaatcag ttatgattga gaaaacagta cttaatatta atgccgcact
     7501 aatgatacca taatctgtcg ttgtaaataa gctggacaag atccacagtg agaccatacc
     7561 aagcccggtt acgcacactg tagacaacaa attccacttc acagcatgta ttgttttcga
     7621 ttttaaattc atatttattg tcagtgatat aaaataatta cattggtgta ctaaaaggaa
     7681 ttttttatac ctttcagcgt tagagcctat cccaaagtaa tgctgccaac ctgttggtga
     7741 ccatttgaat ctgtagccat tcaacccttg taatatcagg tgatcttggc gtcagtgaaa
     7801 atgcgaaatc aataaattgg catcattacc caaaaagcaa taacaatttg ctaaccacta
     7861 cagtttactg ataacatttt gttttcagac aatccctgat gagaaagatc atttataatt
     7921 taccattaaa atgcaaatta accatcttga tggatttgtc attgtttgtt ttttgcaacc
     7981 caaaatatga atagaatttg tttttaacca tcctgattgc ctttataatt aaactgctat
     8041 ttttagatgt taatgtattt attgagctaa gtgtgttata gccatgcaat atttcatgaa
     8101 cacatcgtag tgcatctggg gatgactcga ccttcgtatt ggaaacctct aacattttac
     8161 taatccagat atcatctgtc ttaggtgaaa tagttaaata tgcatcatct ccgataatgc
     8221 taggcttaat cattctctta cttaaaacac aacccccaac gccagttatt ataaaatcct
     8281 tttcaaattt atccttttcc atactaatag aataagagct ataagatttg tacttgttaa
     8341 aaatattcct ctccattatt ctaatcctag cagcaattgc cacattacag ttgttctcat
     8401 aaaactggcc taataggaac tcaagccact gccgaccata tattacatca tcatcagcat
     8461 atacaagaat gtcatcaatc tctgagttaa taattgcgtt aattatttta cggtatggtc
     8521 cggtattttt tacataatgt acattcacta tgttatttat tttatttagc tcttcaaccc
     8581 aagcaggtat ctcacatatc ccatgatctg acaaatatgg ctcatttgat atccaaaggt
     8641 ttattttatc tggctgaaca ctctggttta taattgacca cactgtggcg gcacataaat
     8701 ccagtcgaga atgcattgta gttaaattaa ctgatatcat gctacccttt aaagaaaaaa
     8761 ttaatttaat gttatattat atgtaaaaag gttacatatt caatatgtag gacgtttttt
     8821 cgaaaaaatc agtttttttc ctgatgaacc ttcctccgcg ttgcccggtg atgctgccaa
     8881 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
     8941 gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc actgccggac
     9001 atcagcgcta tctctgctct cactgccgtg aaacatggca gctacagttc acttacaccg
     9061 cctctcagcc cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc
     9121 gggcaaccgc ccgcattatg ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact
     9181 caggccgcag tcggtaacct cgcgcataca accgggcagt gatgtgattg tctgcgctga
     9241 aatggacgaa cagtggggct acgtcggggc taaatctcgt cagcgctggc tgttttacgc
     9301 gtatgacagg atacggagga cggttgtggc gcacgtcttc ggtgaacgca ctctggccac
     9361 actggagtgc tgtcggcctt tgaggtcgtg gtatggatga cggatggctg gccgctgtat
     9421 gaatcccgcc tgaagggaaa gctgcacgtt atcagcaagc gttacactca gcgcattgag
     9481 cgacataacc tgaatctgag acaacatctg gcacggctgg gacggaagtc gctgtcgttc
     9541 tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat aaaacactat
     9601 caataatttt gagtcattac caatttcatc ctgtccctcc gttttctctg tgaggggtca
     9661 gtttggatat agagaattat tgtacggtaa gcctgttttt tgaacaggct tacctgtagc
     9721 aatagggtgt actcccgcgc tatacagcgt tttcagggga ttttgtctta atcggataac
     9781 ctaaatgcga tacagcaaga gcgacaacct aaaagcgata gccactgacg ataaatcaat
     9841 cacttacgta tccgaattgg ttaaataatg tgcttaacgt acaaaaattt ccgatctcca
     9901 aactgacccc gagaaggccc ggaaatatgt ggccggggag cggcataaca ccgtgctggc
     9961 ccgtctggaa gcagagaaag cagaggaaag acggagaaat gcctgcgcga cggcggaacg
    10021 gcgcagacgg aaactggaaa aactccagac cactgacgga acagcagggc aaaaacactg
    10081 aggcatttca ggactgccag aaacgccacc aggcacgggg ccgggtcgtg ttctgccgca
    10141 tctcttcccg gttctccagc aacaacatcg cctgtttcag gctgtcgata tgtgaatctc
    10201 tggaggcaag gagggtgttc aggtgttcaa tttcctgttt cagaatgtaa atgacctgtt
    10261 cattacggtc agaagtgact gtgtcacgct gtccactgac ctgttcaccc ctctgttcaa
    10321 cgggtacagg cgcaaaggca ccaaacaccc gtaaaagctc tgaagtctcg agcattttcg
    10381 cattccgttc accccgacag gtggacagtt caccggcttt aattaacctg tgcagggttg
    10441 tcctgctctt acccgtcagg cgtgcagcct cactgatcga cacgttcgcc atcagtcacc
    10501 ctcctgtagt gcatccagtc tgagggacag tgtacggaaa agatgctccg catcatcgtg
    10561 gagtttctca caaaggtcag cctccgcatc gagatcaagc tcgttaagct tctccagcag
    10621 gagcagggac tgattctgga tgaatttagc catgttcagg gcagttttct gttgtttcgt
    10681 cataaggggc ctcagagcgt cagtgctcag tgtaaaacct gtctcttttc cgcttcctcg
    10741 ctcactcagt cgctacgctc ctgccgttcg gctgcggcga gcggacggga cacacagaag
    10801 agtcatcgct atcaccagga agagaagagc ctgtggaaaa agccacggcg ttgccgtttt
    10861 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagcggtggc
    10921 gaaacccgac aggactttaa agataccagg cgtttccccc tggtggctcc ctcgtgcgct
    10981 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttatctc
    11041 attccacgcc tgacactcag ttccgggtag gcagttcact ccaagctgga ctgtatgcac
    11101 gaaccccccg ttcagtccga ctgctgcgcc ttatccggta actatcgtct tgagtccaac
    11161 ccggaaagac atgcaaaagc gccactggca gcagccactg gtaactgatt tagaggagat
    11221 agtcttgaag ttatgcgccg gttaaggcta aactaaacga acagttttgg ttactgagct
    11281 cctctaacta gccagttacc tcggttcaga aacttgccgg ttgctgaacc ttcgaaaaaa
    11341 ccaccttgcc gggtggtttt ttcgtttact gagcaagaga ttacgatgcc aaagaaagga
    11401 tctcaagagg atcctgtttc atatgcctac ccctgaagtg aaaccagcac cactccgcga
    11461 caccaactat caaaataaaa ttattgaact cattgagttt gataaggaaa aaaacagata
    11521 taatcatgat ccacaatcat ccaactagtc aataaaatca atgaattata ctttctttat
    11581 agttctgacc ttatggtgga ctagactgga cagctgcatt catcacaaac ttaatgagag
    11641 taggaaagat tagagagtac gcccccagta aaacttacat attcttgctg cttacaaccc
    11701 ttccatctag cgttggcgtc ttcatctatc ccatagcgga agtgacctgc ccccaggatt
    11761 agatacaacg ctcagttagt aatgtcggat ccttcactct cagaattacc ctttctccag
    11821 cccgccgcaa attcagacgg cgtctgataa ttcagcgcgg agtgcgggcg gcactcgtta
    11881 taatcctgac gccattcact gatggttttc ctggcatgac tgacgtcgct gaaccagtgt
    11941 tcattcaggc attcatcgcg aaagcgtccg ttaaaactct caataaatcc gttctgtgtc
    12001 ggcttaccgg gctggataag ccgcagctcc acgccatgct cataagccca ctggtcaagt
    12061 gctctgcagg taaactccgg gccctgatca gttcttatcg tcgccggata gccgcgaaac
    12121 agcgcaatgc tgtccagaat acgcgtgacc tgcacgcctg aaatcccgaa ggcaacggtt
    12181 accgtcaggc actccttcgt gaagtcatcc acgcaggtca ggcacttgat cctgcgaccg
    12241 gtggccagcg catccatgac aaaatccatc gaccaggtca gattaggcgc cgccgggcgg
    12301 agcagcggca gacgttctgt tgccagccct ttacgacgtc gtctgcgttt tacgcccagc
    12361 ccactgagat ggtagagccg gtatactcgc ttgtgattaa catgaagccc ttcgcggcgc
    12421 agcagctgcc agatgcggcg gtagccaaaa cgcctgcgct ccagtgccag ttcggtgatg
    12481 cgtccggata aatgcgcatc cgatgtcgga cgctgcgctt cgtaacggca ggtcgacagg
    12541 gacaaacctg ttagcctgca ggcacgacgt tgcgacagac cggtcgtatc acacatcaac
    12601 accacggctt cccgcttctg gtctgtcgtc agtactttcg gcccagagcc acctgaagcg
    12661 cctccttatc cagcatggct tcggccagca gcttcttgag tcgggcgttc tcttcctcaa
    12721 gcgacttcag gcgcttaacc tcgggcacct ccataccgcc atacttttta cgccaggtgt
    12781 aaaacgtggc gtcggaaatg gcgtgcttgc ggcagagttc acgggcggat accccggctt
    12841 cagcctcgcg gagaatactg atgatctgtt ggtcggaaaa acgcttcttc atggggatgt
    12901 cctcatgtgg tttatgaaga cattactaac atcggtgtgt actaatcaac ggggagcagg
    12961 tcagaagcta tatggggtga aaccgggc
//
