LOCUS       CP056504                3354 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Klebsiella oxytoca strain RHBSTW-00452 plasmid pRHBSTW-00452_10,
            complete sequence.
ACCESSION   CP056504
VERSION     CP056504.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148619
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00452
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 79.4092762823611x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:06:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,549
            CDSs (total)                      :: 6,431
            Genes (coding)                    :: 6,255
            CDSs (with protein)               :: 6,255
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 59 of 176
            Pseudo Genes (incomplete)         :: 109 of 176
            Pseudo Genes (internal stop)      :: 27 of 176
            Pseudo Genes (multiple problems)  :: 18 of 176
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00452"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:571"
                     /plasmid="pRHBSTW-00452_10"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(536..937)
                     /locus_tag="HV202_32740"
     CDS             complement(536..937)
                     /locus_tag="HV202_32740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLT68533.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(937..1128)
                     /locus_tag="HV202_32745"
     CDS             complement(937..1128)
                     /locus_tag="HV202_32745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLT68534.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFCTLTARLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1177..1598
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.356"
                     /coverage="82.0"
                     /evalue="4.8100000000000005e-133"
ORIGIN
        1 atccgaaaaa aaaacggtga acggggacgc aatttttatt gcgtggccgt gagcgcgttg
       61 ccggcgcagc cggaaaaccg gttttcagca cgttatggga atcgggttcg tcgacggccg
      121 gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg cgattcgaat
      181 cgtgaattga atcaagagtc accatgagca aaaaacaccc aaagtgatga attgaatcaa
      241 gagtcgattc atcataacaa tatgataata aagcgtgatt cacatatgaa tcgtgggcaa
      301 ttcgtcagcg attcgccata gagtcgtcca cgacttggat ctgcactttg taacacatca
      361 ccgcacattt atttttcatt aacccattgt tgtaaataag gaatctgcaa aattttaatt
      421 ttgcgtgggg tgtgggtatt tttagtggtg aggagcgttc agagatctga cgatttttgg
      481 gaaggttcag gaactccctg ggatggccgg agaggaaaac tgaagcatga cggggtcagg
      541 attttttcca gaatttccac catggcgccg gggattcagt taattttgcc tggtgatttt
      601 caagaaggag catggcctgt ttcaggctgt caatgtgcga atccttggac aaaagtaagg
      661 tgttcaggtg ttcaatctcc tgtttcagat gacgaatcac ctgttcactc tggtcagcag
      721 aggctgtatc acgctgttca gagacctgtc cagaatggtg ttcacccttc agttgttcaa
      781 aggggccaaa gacacgtaaa agctctgaag tatctaccat ttttgcatta cgttcaccgc
      841 tacaggtgga caggtcacct gctttaatta acctgtgcag tgttgttcta ctcttacccg
      901 tcagtcgggc agcctcacta atagacactc tggccatcag ttaccatcct gtaatgcatc
      961 cagtctcgcg gtcagtgtac agaaaagatg ctccgcatca tcgtggagtt tctcacaaag
     1021 gtcggcctcg acatcgagat caagctcgtt aagcttctcc agcaggagca gagactgatt
     1081 ctggatgaat ttagccatgt tcagcgctgt tttctgttgt ttcgtcataa agggcctcag
     1141 agcgtcagtg ctcaatgtca gacagatctc ttttccgctt cctcgctcac tcagtcgcta
     1201 cgctcctgcc gttcggctgc ggcgagcggt cgcaacgcgc acataacgac atagagcaga
     1261 cagctcggag agggagaagg cagcacagcg gccgtttttc cataggctcc gcccccctga
     1321 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     1381 ataccaggcg tttccccctg atggctccct cgtgcgctct cctgttcctg ccattcggtt
     1441 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     1501 ccgggtaggc agttcgctcc aagctggact gtatgcacga acccccgttc agtcagtccg
     1561 actaccacgc tcttttcggt aactatcgtc ttgagtcctt tcgttttcag agcaagagat
     1621 tacgacgctc ctgaaaggat ctcaagaaga tccttttgaa ctttggttca tttcttcgga
     1681 ccccccccat acatgctcgt caatcttttt ctatcatcac catagatatt cttttgaata
     1741 aaagaggtta attgtatgtg aacgaggagg aagtattgtc atcaatattg attactctat
     1801 cgccacagac tctaagctca tcatttggtt gccagtgagc aacatcaaca atattgtcaa
     1861 ctacatgcag agttactccg tcgctcgtga ctaaaagcct accgtcttct gtcctagctt
     1921 taacatacgt tgtatcacaa ctatccgatg aggattgaac aggaaaccca aaattaggtt
     1981 gagggtgctc tgatgccttc aaaactcctc ctgtcagacg gtcataaacg gtgccatcag
     2041 gagttacagc cgaatttcta taaggatcat atactctgtc aggaatatcg tgttgtcatg
     2101 aatatgctat cggtgatata gtatgctatc ggtgatatag ccaaaaaaag taatgaccga
     2161 gatgaaaaac agacttactt cataatttcc attttttttt aaaaaaaata tgcaattaaa
     2221 actaaaggcc acaaaaacgc acaaattatg ccgacaagca ttgacgcacc ttcttcggtg
     2281 taacttctgc gcctcccgtt cctaacttct tggataaaca ggaaaccggc actcacaata
     2341 aggggtcgct tcaggaaacg gaaaataaag cacgctaagc cgtcgttccc cacctgctgc
     2401 cagctctggt atgacgttgg cggtgtcagt ctggggcatg ccccaactgg acgtcagacc
     2461 agagaggtaa aaccgtcaga gtagagccgt tttccgaatt tgtggacacc attgatgcgc
     2521 ggtctatggc cacaacctga caccttgtgt cccttagcgt acgatttttc catagctaac
     2581 ctttcgaaaa taaacattgt aagtgggatt aaaaatcaca atataaattt acaaatacaa
     2641 agtaaaaaga agatgtaatt aatgatctgt taaatggccg tggaaaagaa agttatctaa
     2701 gtctctctat taatgaaaat atcattaaaa aacaaagaga tagaaacaaa tagcattaaa
     2761 agaaatacat taactttcag tggcatatcc agtttcaata catttaacgt aaaaaaaaaa
     2821 aagtggtaaa tagtcatggt taagaaaaaa aagaccaaat agtatacggc gacaatcaca
     2881 agactgttta ttgtgatcat ttagtaagat ttggatttgg cccagtcgta tcgaagttag
     2941 actttggaac tattagtgat atagattctg gaaatgaaca agtatctata cagacaacca
     3001 taataattcc aacaccaaat ttaatagatg taataccgct attaaatgat caatcaaaaa
     3061 aaccagaagt aagaggagag atcatatcaa aattaaaaag aaatgatatc taatcttgaa
     3121 gaagaaaaag agtgattaaa tagagtatga aataaattta aatatcattt accataaata
     3181 acatccgtta ttggcacgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc
     3241 gccagaaacg cgcaggctct gtttttaagg catgcatgag tatgcacgca agtgcatagc
     3301 gcgaaaaatt cacgccattc agccagagta agtcctgttt ccggcgttct ttcc
//
