LOCUS       CP056502                9690 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Klebsiella oxytoca strain RHBSTW-00452 plasmid pRHBSTW-00452_8,
            complete sequence.
ACCESSION   CP056502
VERSION     CP056502.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148619
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00452
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 79.4092762823611x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:06:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,549
            CDSs (total)                      :: 6,431
            Genes (coding)                    :: 6,255
            CDSs (with protein)               :: 6,255
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 59 of 176
            Pseudo Genes (incomplete)         :: 109 of 176
            Pseudo Genes (internal stop)      :: 27 of 176
            Pseudo Genes (multiple problems)  :: 18 of 176
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00452"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:571"
                     /plasmid="pRHBSTW-00452_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(9399..9690,1..232))
                     /locus_tag="HV202_32660"
                     /pseudo=""
     CDS             complement(join(9399..9690,1..232))
                     /locus_tag="HV202_32660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(635..1567)
                     /locus_tag="HV202_32665"
     CDS             complement(635..1567)
                     /locus_tag="HV202_32665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QLT68527.1"
                     /translation="MLLSIIIPCYNCESNIEKLLLSIKEQLNETVQLVLINDGSWDATH
                     DIITKFTSDNPSIKIKYINSINQGAAKARSTGLNYAEGDYVFFCDSDDMISANFISVFT
                     ELSNDKPDMIYFCSTVLDSQGNRFKKVHFPLAYIESNSNEVFNYLLKKGQWTAAVWTYI
                     FRKDLVLKSGAIFTDRKVHEDHLFTLRLLAGSEKISICNSELYFQNVTSGSLTKSPKKI
                     DYLTERYKSYKEAREDIIYKFSQESIVLYDRWSMSSLLQILKENKKMVFQGIMHTAIYR
                     VLWKEKLVILDFVKSKVKKKITRSKSLSQ"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(1567..3033)
                     /locus_tag="HV202_32670"
     CDS             complement(1567..3033)
                     /locus_tag="HV202_32670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="QLT68519.1"
                     /translation="MNLKSKTIHAVKWNLLSTVCVTGLGMVSLWILSSLFTTTDYGIIS
                     AALILSTVFSIITDFGISNSVIRSEKLNKYELSSLYIINVFLGMIFCLGLFIFSAQLAS
                     LFNGGTELAKQIKIMSFSLIISSFGGQPRALLARELRFDVISRITILSAIFNFVFAVFL
                     SYVYRSPWCVAVSLLVSSSISSVLYIYSSKGLRDIKLQFFTLKEIKKHVSYGSQLVMDS
                     IINQISVNTYPVLMARLISLTAIGGYNISYNISVALFEKLNPVLSHALFPAFSKIQNDA
                     VSLKKTFLKVTMFSSLVNFPMLLGMMLISDYVVTVFFEQKWQFITPIVQILCLVGAARS
                     LDVPVISVLLAKGQMYRNVYLGVFKLALGIPLTWFLGKQYGLTGIVFSFLCIQIVNLVM
                     GYFFQVRPCLNIKVKSYIYAVFVPLIHTLPMLVTGYLLILFLTITSPILSMVVIIFSCM
                     SVYIASILTSPVKEIKEFNAIVLNNILRNK"
                     /name="MOP flippase family protein"
     gene            complement(3314..4141)
                     /locus_tag="HV202_32675"
     CDS             complement(3314..4141)
                     /locus_tag="HV202_32675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QLT68520.1"
                     /translation="MISVNLTTMHSRLDLCAATVWSIINQSVQPDKINLWISNEPYLSD
                     HGIYEIPAWVEELNKINNIVNVHYVKNTGPYRKIINAIINSEIDDILVYADDDVIYGRQ
                     WLEFLLGQFYENNCNVAIAARIRIMERNIFNKYKSYSSYSISMKKDKFEKDFIITGVGG
                     CVLSKRMIKPSIIGDDAYLTISPKTDDIWISKMLEASNTKVESSPDALRCVHEILHGYN
                     TLSSINTLTSKNSSLIIKAIRMVKNKFYSYFGLQKTNNDKSIKMVNLHFNGKL"
                     /name="glycosyltransferase family 2 protein"
     gene            4323..5020
                     /locus_tag="HV202_32680"
                     /pseudo=""
     CDS             4323..5020
                     /locus_tag="HV202_32680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            5567..5788
                     /locus_tag="HV202_32685"
     CDS             5567..5788
                     /locus_tag="HV202_32685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010365093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLT68521.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNA"
                     /name="hypothetical protein"
     gene            5781..6668
                     /locus_tag="HV202_32690"
     CDS             5781..6668
                     /locus_tag="HV202_32690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="QLT68522.1"
                     /translation="MPKFASAARQATGVIKTLQGDILRSVGTVRNYEQGLTRVAEWMKD
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSPLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            6665..7363
                     /locus_tag="HV202_32695"
     CDS             6665..7363
                     /locus_tag="HV202_32695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLT68523.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRSSESRRIQSETDRYQQALREAEEAHAARQNALI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQTVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKQQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(7370..7768)
                     /locus_tag="HV202_32700"
     CDS             complement(7370..7768)
                     /locus_tag="HV202_32700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="QLT68524.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFAPVSVEQAEEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTNRPWWRFWQS"
                     /name="DNA-binding protein"
     gene            complement(7768..7959)
                     /locus_tag="HV202_32705"
     CDS             complement(7768..7959)
                     /locus_tag="HV202_32705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLT68525.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSFRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8371..8484
                     /locus_tag="HV202_32710"
     CDS             8371..8484
                     /locus_tag="HV202_32710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLT68526.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          8008..8484
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.304"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 acaaccgtct tccggagcct gtcatacgcg taaaacagcc agcgctgccg cgatttagcc
       61 ccgacgtagc cccactgttc gtccatttcc gtgcagacga tgacgtcact gcccggatgt
      121 atgcgcgagg ttaccgactg cggcctgagt tttttaagtg acgtaaaatc gtgttgaggc
      181 cagcgcccat aatgcgggcg gttgcccggc atccaacgcc attcatggcc ataggggtca
      241 gtttggagat cggaaatttt tgtacgttaa gcacattatt taaccaattc ggatacgtaa
      301 gtgattgatt tatcgtcagt ggctatcgct tttaggttgt cgctcttgct gtatcgcatt
      361 taggttatcc gattaagaca aaatcccctg aaaacgctgt atagcgcggg agtacaccct
      421 attgctacag gtaagcctgt tcaaaaaaca ggcttaccgt acaataattc tctatatcca
      481 aactgacccc tataacggca aatagtaatg ctgccaacct gttggtgacc atttgaatct
      541 gtagccattc aatccttgta acatcaggtg atcctggcgt cagtgaaaat gcgaaatcaa
      601 caaattggca tcattaccca ttatacgata atccctattg ggataggctc ttagatctcg
      661 tgattttttt tttaactttg cttttaacaa aatctaaaat cactaatttt tccttccaca
      721 atactcgata tatagctgta tgcattatcc cctgaaacac cattttttta ttttctttta
      781 atatttgcag tagcgaagac atagaccatc gatcataaag aacaatgctt tcctgactga
      841 atttgtaaat gatatcttcc ctggcctctt tataagactt ataacgctca gttaaataat
      901 caattttttt aggggattta gtaagtgaac ctgaagtcac attctgaaag taaagctcag
      961 aattacatat tgatattttt tcactaccag cgagaagtct cagcgtgaat aagtgatctt
     1021 catgtacctt tctgtctgta aatatagcgc cagatttcaa tacgagatct ttccggaaaa
     1081 tataagtcca tacagcagct gtccactgcc ctttcttcaa aagataatta aaaacttcgt
     1141 tactattgct ttcgatgtat gctaatggaa aatgtacctt tttaaacctg tttccttgcg
     1201 aatctaatac cgtagagcaa aaatatatca tatccggttt atcattactt aactctgtaa
     1261 aaaccgatat gaaattagca gaaatcatat catctgaatc acagaagaat acataatctc
     1321 cctctgcgta atttaagccc gttgatctgg cttttgccgc cccctgatta atactattaa
     1381 tgtattttat ttttatagag gggttgtcac ttgtaaattt agttattatg tcatgcgttg
     1441 cgtcccatga gccatcatta atcaacacta gttgtacggt ttcattaagc tgctctttta
     1501 ttgagagaag caatttttcg atatttgact cacagttata acatggtatg attattgata
     1561 ataacatcat ttattcctca gtatattgtt tagaacgatt gcgttaaatt cctttatctc
     1621 ttttactgga gaggtgagta tagaagcaat gtatacagac atacatgaaa aaataatcac
     1681 aaccattgac aaaataggag aggtaatagt taaaaaaaga atcaacaggt aacccgttac
     1741 aagcataggt aaagtgtgta taagcggtac gaagacggca tagatgtaac tctttacttt
     1801 gatatttaaa cagggtctaa cctgaaaaaa atatcccata acaagattta ctatttgaat
     1861 acataagaat gaaaacacta tccctgtaag accatattgt ttacccagaa accatgttaa
     1921 aggaatacca agcgctaact taaatactcc caggtaaaca tttctgtaca tttgcccttt
     1981 agcaagtaag acagagataa caggcacatc aagggatcgc gccgccccaa ccaaacataa
     2041 aatctgtaca ataggggtga tgaactgcca tttctgctca aaaaaaacag tcactacata
     2101 gtctgaaatt aacatcattc ccaataacat cgggaaattc accaaagacg aaaacatagt
     2161 tacttttaga aaagtttttt tcagagatac tgcatcattt tgtatcttgg aaaaagctgg
     2221 aaataacgca tgtgataaaa caggattcaa tttttcaaaa agcgccacgc taatgttgta
     2281 ggatatatta tatccaccga tagcagtcaa acttattaag cgtgccatta gtacaggata
     2341 tgtattcacg cttatctgat taatgataga gtccatgacc aactgagaac cgtagctgac
     2401 gtgttttttt atttccttca aagtaaagaa ctgtaactta atatctctca agcctttgct
     2461 tgaatatata tacaatacgc tactaataga tgaggaaacc aacagtgaaa cggcaacaca
     2521 ccagggggaa cgatagacgt agcttaagaa gactgcgaac acaaaattaa aaatagctga
     2581 caggattgtt atcctggata ttacatcaaa tctaagttct ctggctaaca aagctcttgg
     2641 ctgtccaccg aaagacgata ttataagaga aaatgacatg atttttatct gttttgccag
     2701 ttctgttcca ccattgaata aggatgctaa ctgagcacta aaaataaaca agccgagaca
     2761 aaatatcata cctaaaaaga catttattat atacagtgaa gacagttcat atttatttaa
     2821 cttttcagat cttataactg agtttgatat accaaaatca gttatgattg agaaaacagt
     2881 acttaatatt aatgccgcac taatgatacc ataatctgtc gttgtaaata agctggacaa
     2941 gatccacagt gagaccatac caagcccggt tacgcacact gtagacaaca aattccactt
     3001 cacagcatgt attgttttcg attttaaatt catatttatt gtcagtgata taaaataatt
     3061 acattggtgt actaaaagga attttttata cctttcagcg ttagagccta tcccaaagta
     3121 atgctgccaa cctgttggtg accatttgaa tccgtagcca ttcaaccctt gtaatatcag
     3181 gtgatcctgg cgtcagtgaa aatgcgaaat caataaattg gcatcattac ccaaaaagca
     3241 ataacaattt gctaaccact acagtttact gataacattt tgttttcaga caatccctga
     3301 tgagaaagat catttataat ttaccattaa aatgcaaatt aaccatcttg atggatttgt
     3361 cattgtttgt tttttgcaac ccaaaatatg aatagaattt gtttttaacc atcctgattg
     3421 cctttataat taaactgcta tttttagatg ttaatgtatt tattgagcta agtgtgttat
     3481 agccatgcaa tatttcatga acacatcgca gtgcatctgg ggatgactcg accttcgtat
     3541 tggaagcctc taacatttta ctaatccaga tatcatctgt cttaggtgaa atagttaaat
     3601 atgcatcatc tccgataatg ctaggcttaa tcattctctt acttaaaaca caacccccaa
     3661 cgccagttat tataaaatcc ttttcaaatt tatccttttt catactaata gaataagagc
     3721 tataagattt gtacttgtta aaaatattcc tctccattat tctaatccta gcagcaattg
     3781 ccacattaca gttgttctca taaaactggc ctaataggaa ctcaagccac tgccgaccat
     3841 atattacatc atcatcagca tatacaagaa tgtcatcaat ctctgagtta ataattgcgt
     3901 taattatttt acggtatggg ccggtatttt ttacataatg tacattcact atgttattta
     3961 ttttatttag ctcttcaacc caagcaggta tctcatatat cccatgatct gacaaatatg
     4021 gctcatttga tatccaaagg tttattttat ctggctgaac actctggttt ataattgacc
     4081 acactgtggc ggcacataaa tccagtcgag aatgcattgt agttaaatta actgatatca
     4141 tgctaccctt taaagaaaaa attaatttaa tgttatatta tatgtaaaaa ggttacatat
     4201 tcaatatgta ggacgttttt tcgaaaaaat cagttttttt cctgatgaac cttcctccgc
     4261 gttgcccggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     4321 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     4381 acggcaaaag cactgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc
     4441 agctacagtt cacttacacc gcctctcagc ccggtacgca ccagaaaatc attgatatgg
     4501 ccatgaatgg cgttggatgc cgggcaaccg cccgcattat gggcgttggc ctcaacacga
     4561 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac atccgggcag
     4621 tgacgtcatc gtctgcacgg aaatggacga acagtggggc tacgtcgggg ctaaatcgcg
     4681 gcagcgctgg ctgttttacg cgtatgacag gctccggaag acggttgttg cgcacgtatt
     4741 cggtgaacgc accatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt
     4801 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
     4861 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca
     4921 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa ttggtggagc tgcatgacaa
     4981 ggtcatcggg cattatctga acataaaaca ctatcaataa ttttgagtca ttaccaattt
     5041 catcctgtcc ctccgttttc tctgtgacgt cattctggca tcacgacggt caccgttggc
     5101 ggggtccggg gcggtcagcc ccggcgttct ttattcaggc aatatgagca cctgcgacac
     5161 ccgctatgta cgacgcatta atgcgcctgt acatagcggg tgtcgctatt aaactgagtc
     5221 aggcgcaaca tttaaaaaac aacattcaca cgcatgcaaa acctggctag tatttagtca
     5281 aaccatgaga ctgcgcactc tgacgcgcac ctgcctaccg gcagcaggtt aaacagccgg
     5341 gtccccccgg ggatactgta ccggacagcc caatccgaga acactgagac aacagcccaa
     5401 atctgtcaga cccacaaccg ggtcaagtca ttctctgact aaagagacgt tgagccgcga
     5461 gcagtccggg cttaaccacg ttacggttta aacgagggcg atcggcgcag gccgcagtga
     5521 ccgttaacgg ccactgatgt tcttacagtc ccactccgga gcacagatgg aatttgatac
     5581 cgccgccgaa ctggccgccc tgcaggcgca gacccgccgt atccgccagg tccgctaccg
     5641 tccctcccgc ctcgaccgct ataccggcga actgctgagc ctgtaccagg ccggggcgag
     5701 tgcagccgag ctgcaacgct ggctccgggc ccggagaatc aaagtcgtgt tgtccacggt
     5761 tacccgctgg ctggaaaaaa atgcctaaat tcgccagcgc tgcccgccag gcgacgggcg
     5821 tgataaaaac cctccagggc gatattcttc gctctgtcgg tacggtccgg aattacgagc
     5881 agggcctgac ccgcgtcgca gaatggatga aagacaaccg gattaatcac ggcgatttac
     5941 gcacactgac accggcgcag gccatcacct acctggagca gcgcggggag caggtcggcc
     6001 agaaaacgct cgatatggaa cgccaggcca tccaggccat gatgatccac gtgacgcaga
     6061 ccctccctgc cggcgaacac ctgccggtgg tgaaatcgga gcaggcacag atcctgagca
     6121 gccgggccta cagcccggac caggtggccc tgattgccgc tgcacaacag cccgccaacg
     6181 ctctggccac cgcgattgct ctggccgccg gattacgcgc ccatgaactg ctgaccctcg
     6241 cgccccgcca ggagcgagca ccggatcccc gtccggcagc ggacagtaaa tttgccggtc
     6301 gggagggtgt gatttacacc gttcagggca aaggcggcct ggtcagggaa gtcctgctgc
     6361 cgtcccccct agctgaacgt ctggagcatg tcaggctggc gagcccggtc cgggtgaccg
     6421 atcgcggcgt gttttaccag agtcagtacg ccattggcgg agggcagcgc tggtcgaatg
     6481 ccttcagtgc cgcctccatg cgtaccctgg gctggtcacg gggggcacat ggcgtgcgtc
     6541 acagctacgc ccagcaacgg atgcaggagc tgcaaaaact cggcctggtt cgtgatatgg
     6601 ccctgcgtac cgtcagccag gaaatgggcc attttcgtcc ggaaatcacg gagacatacc
     6661 tgcgatgaca agaacgttta ttttattgtg taccggtgtt ttcgccatcg cgtacgccct
     6721 gtatgagctg aaacatatgg gcgtttacta tccggatgcc agcaatccgc agctgttcac
     6781 tggctggaag gcggtcaccg aactcggtcc agcaatcctg gctaccggga tcgcagccac
     6841 agcgttctcg ctgtggctgt tcattttgtt tttcagaagc tcagagtccc gccggataca
     6901 gagcgagaca gacaggtatc agcaggcact gagggaggca gaagaggcgc atgccgccag
     6961 acaaaatgcc cttattcaga atcacaacct ggagacagcg gccctgcgaa gccactacag
     7021 tggtgctgag gatgtactgc gggaagcgtt tgcgcaaaaa gaaagcctct tgcttcagac
     7081 ggtgcagaat gcccggaaag aagccagaaa ggagctggaa gaacagaggg aggcactgaa
     7141 tcagcgcgaa tccatcctgc agaaaaagga gctggcgctg gaaacaaccc tggagaaggc
     7201 ccggaaatat gtggccgggg agcggcataa caccgtgctg gcccgtctgg aagcagagaa
     7261 agcagaggaa agacggagaa atgcctgcgc gacggcggaa cggcgcagac ggaaactgga
     7321 aaaacagcag gccactggcg gaacggcagg gcaaaaacac tgaggcattt caggactgcc
     7381 agaaacgcca ccaggggcgg ttcgtgttct gccgcttctc ttcccggttc tccagcaaca
     7441 acatcgcctg tttcagactg tcgatatgtg aatctctgga ggcaaggagg gtgttcaggt
     7501 gttcaatttc ctgtttcaga atgtaaatga cctgttcatt acggtcagaa gtgactgtgt
     7561 cacgctgtcc actgacctgt tcttccgcct gttcaacgga tacgggcgca aaggcaccaa
     7621 acacccgcaa aagctctgaa gtatcgagca ttttcgcatt ccgttcaccc cgacaggtgg
     7681 acagttcacc ggctttaatt aacctgtgca gggttgtcct gctcttacct gtcaggcgtg
     7741 cagcctcact gatcgacact ttcgccatca gtcaccctcc tgtagtgcat ccagtctgaa
     7801 ggacagtgta cggaaaagat gctccgcatc atcgtggagt ttctcacaaa ggtcggcctc
     7861 aacatcgaga tcaagctcgt taagcttctc cagcaggagc agggactgat tctggatgaa
     7921 tttagccatg ttcagggcag ttttctgttg tttcgtcata aggggcccca gagcgtcagt
     7981 gctcagtgta aaacctgtct cttttccgct tcctcgctca ctcagtcgct acgctcctgc
     8041 cgttcggctg cggcgagcgg acgggacaca cagaagagtc atcgcgataa ccaggaagag
     8101 aagagcctat ggaaaaggcc acggcgttgc cgtttttcca taggctccgc ccccctgaca
     8161 agcatcacaa aatctgacgc tcaaatcagc ggtggcgaaa cccgacagga ctataaagat
     8221 cccaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     8281 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     8341 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc
     8401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcgcca
     8461 ctggcagcag ccactggtaa ctgatttaga ggagatagtc ttgaagttat gcgccggtta
     8521 aggctaaact aaacgaacag ttttggttac tgagctcctc taactagcca gttacctcgg
     8581 ttcagaaact tgccggttgc tgaaccttcg aaaaaaccac cttgccgggt ggttttttcg
     8641 tttactgagc aagagattac gatgccaaag aaaggatctc aagaggatcc tgttccatat
     8701 gcctacccct gaagtgaaac cagcaccact ccgcgacacc aactatcaaa ataaaattat
     8761 tgaactcatt gagtttgata aggaaaaaaa cagatataat catgatccac aatcatccaa
     8821 ctagtcaata aaatcaatga attatacttt ctttatagtt ctgaccttat ggtggactag
     8881 actggacagc tgcattcatc acaaacttaa tgagagtagg aaagattaga gagtacgccc
     8941 ccagtaaaac ttacatattc ttgctgctta caacccttcc atctagcgtt ggcgtcttca
     9001 tctatcccat agcggaagct atatggggtg aaatcgggct tttgagtatc tttggctata
     9061 cagtcctatc ccttattacc ggagtaatca tagggaatgt aatcgttcgg gtgttatcta
     9121 caacagaact cggaatgttg ttcgcaaatc cattagcgat gatatgtagc attaccgggg
     9181 taatagtcgt agctgtacaa tatttaccat aacattcctt gcgcgcacca ccaccacacc
     9241 actcatcgcc aaaatccgcc ggaacgtcca aaagtgcagg ttttacacac cgaaatcggg
     9301 ctatttttgt gttgccggtt tgttatcaaa atggcgagac tggtttcagc ctgtttacag
     9361 actgaaacct ctatttcttc cagggtaatg actcaaaatt attgatagtg ttttatgttc
     9421 agataatgcc cgatgacctt gtcatgcagc tccaccaatt ttgagaacga cagcgacttc
     9481 cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta
     9541 tatcgcttgc tgattacgtg cagctttccc ttcaggcggg attcatacag cggccagcca
     9601 tccgtcatcc atatcaccac gtcaaagggt gacagcaggc tcataagacg ccccagcgtc
     9661 gccatggtgc gttcaccgaa tacgtgcgca
//
