LOCUS       CP056482                1887 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00466 plasmid pRHBSTW-00466_2,
            complete sequence.
ACCESSION   CP056482
VERSION     CP056482.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148623
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00466
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.1452575582334x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:27:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,174
            CDSs (total)                      :: 5,052
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 92 of 199
            Pseudo Genes (incomplete)         :: 94 of 199
            Pseudo Genes (internal stop)      :: 56 of 199
            Pseudo Genes (multiple problems)  :: 38 of 199
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00466"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00466_2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            192..428
                     /locus_tag="HV206_25865"
     CDS             192..428
                     /locus_tag="HV206_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLX85748.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1269..1382)
                     /locus_tag="HV206_25870"
     CDS             complement(1269..1382)
                     /locus_tag="HV206_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLX85749.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(1274..1757)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.277"
                     /coverage="80.0"
                     /evalue="0.0"
ORIGIN
        1 gactggttca gggctgcgcc ccgaaacccg ctaaaaccac tgacgcgcct gcggcttgcc
       61 caacgtctca cgtcaccgga ttaaaatccg ctcccgttca ccgtttctgg ctgtctttca
      121 gcaccgccag cgcttcatcg gttctgctgt gtctggcaga ctgtgcggag ttattctgac
      181 gggagaatgc tatgaacaga ctgacaaacc tgacacctgc agagaaaaaa tttcttgatg
      241 atgctattgc cgcagctgag agagcgtcag gcaaaaagct aaatcagccg aaccggcata
      301 tcgtcctgaa cagagcgcgt gcgcagattg agtcgcagcg ttacgccgac cgccagcgtg
      361 cattacgtga ggatgagcgg cagcagtccg agtttgcctg gtcacgtccg cgcgcgccgc
      421 gccgctgatt gcgccttttg gcggtttagt ggtgctgaca ctccgaaatc gtctgccatt
      481 cacccgcgtg catagccatg cagtcgcgca gaaatcatca ggtgcgtttt tcagggttat
      541 ggccagggaa aggtgctgga aatggctaac tcgttacaga gtggcgctga gggcctctca
      601 ggcggtgcgg gcaacggatg ttatggtaaa tagagcgttt tggtgactcg cccgccacat
      661 ctttagctgt gatataattt tacagaagga tgaaagggca aaaaaaaaga atccgtaagg
      721 attctttttc tcgcctcagt cagcgactgc gaatcgctga ctgctacccg aaaacatgta
      781 ccgaagctca cgttgtcgtt acttatcaca aagtaatttt acccctgggg tgaatctacg
      841 tcaattgagc cggtattcct ttctatcaat atgaaggaat aaacgtatgg ccgaactgct
      901 tacggcgttt attaaagcac cacttataag ccaggtgctg accatcattc tgtgtctgat
      961 ctttgtagta cacagtcttt taaattgatg taccacccat aaaaaaagcc acttatgtgg
     1021 ctttttttat gaatcgagag ataggtatct gttgaatatc acagatgatc ttcttgagat
     1081 ccttttcgtt gcgtcgtaat ctcttgctct ggaaacgaaa aaaccgccca gcaaggcggt
     1141 ttttcgaagg ttctcagagc agcaactctt tgaaccgcgg taactggctt aagaggagcg
     1201 cagtaaccaa atctgtcctt tcagtttagc cttaactggc acataacttc aagactaact
     1261 cctctaaatc agttaccagt ggctgctgcc agtggcgctt ttgcatgtct ttccgggttg
     1321 gactcaagtc gatagttacc ggaacaggcg cagcagtcgg actgaacggg gggttcgtgc
     1381 atacagtcca gcttggagcg aactgccttc ccggaactga gtgtcaggcg tggaatgaga
     1441 gaccgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     1501 acgagggagc caccaggggg aaacgcctgg tatctttaag tcctgtcggg tttcgccacc
     1561 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     1621 gctttgccgc ggccttatgg tttccctgtt aagtttcttc ctggcatcca ctaggaaatt
     1681 tccgccccat tagtaagcct gcaccgctcg ccgcagccga acgaccgagc ggagcgagtc
     1741 agtgagcgag gaagcggcat ttctccggca gtgcatcttc tgctgacgca tacgaatctc
     1801 atttttcacc tgctttatgt ggcagctatg cctgatgttt ctgcgatgtt acattttaag
     1861 ccagtataca ctccgctagc gctatgt
//
