LOCUS       CP056480                4183 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00473 plasmid pRHBSTW-00473_7,
            complete sequence.
ACCESSION   CP056480
VERSION     CP056480.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148625
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00473
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 102.372331253518x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 01:41:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,902
            CDSs (total)                      :: 4,787
            Genes (coding)                    :: 4,649
            CDSs (with protein)               :: 4,649
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 49 of 138
            Pseudo Genes (incomplete)         :: 86 of 138
            Pseudo Genes (internal stop)      :: 20 of 138
            Pseudo Genes (multiple problems)  :: 15 of 138
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00473"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00473_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3774..4183,1..118))
                     /locus_tag="HV208_24490"
     CDS             complement(join(3774..4183,1..118))
                     /locus_tag="HV208_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU38095.1"
                     /translation="MIIVKKTLAIYLDLLRSIQKKRDQLQRLNVLNVVIHSLSYSVEVF
                     NMQYRGLEFEEQEFNDCLNILHKEVVSFLNKNNMALDFFQYPVEDICRIIFRDKIDLRT
                     AVILDFGITTYDLSPLGDHDPFVWRLLEWPLYDYENFQFVIVQKLASYELDKLNEIIQV
                     EDNKDNKDKRRL"
                     /name="hypothetical protein"
     gene            403..1596
                     /locus_tag="HV208_24495"
     CDS             403..1596
                     /locus_tag="HV208_24495"
                     /inference="COORDINATES: protein motif:HMM:NF015354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLU38096.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYVDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="MobA/MobL family protein"
     gene            2134..2991
                     /locus_tag="HV208_24500"
     CDS             2134..2991
                     /locus_tag="HV208_24500"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015076.1,HMM:NF020291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLU38097.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEKVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQEKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPLNPLQYKELKATARSIARWTWKNFDSHTFSLIQSSRGAKNKNKPKKSNVKEMLGFI
                     GGEK"
                     /name="replication initiation protein"
     gene            2988..3257
                     /locus_tag="HV208_24505"
     CDS             2988..3257
                     /locus_tag="HV208_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="QLU38098.1"
                     /translation="MRRSTIRKGLTQKEMAERFNISVKTVKKYTSEPREQYEQTAADRR
                     RQAYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPKNSL"
                     /name="response regulator transcription factor"
     gene            complement(3503..3772)
                     /locus_tag="HV208_24510"
     CDS             complement(3503..3772)
                     /locus_tag="HV208_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="QLU38099.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LDKKPEEINKALILGLLSKINELDEEEKRDFTRIGMDILQQWKS"
                     /name="conjugal transfer protein TraD"
     Typing          404..1413
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.703"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 aagatagtga atgtattacc acatttagta cattcaacct ctgtaattgg tccctcttct
       61 tttgaatact cctcagtaaa tctaaatata tggcgagtgt ttttttcaca atgatcacac
      121 caattgactt gtatatcagg gccgagcgtt tctttttcca tataacctcc attattttat
      181 tttgttattt attacctcca ttatttactt tagtcaaata tattttagaa taaaataaat
      241 aatatttaat attgggcgaa gcctggggtt cattacttat tattttgagg tgaagccgaa
      301 aaaataatat tagtaatgcc cacttacacg tcgcaagcga cgtatgggcc ctgcggggct
      361 aaaaaccaaa attcaaaaga caaaaaaagt aaggggtaga ctatggcgac atttcatatc
      421 agctttaaaa aattacgaag atcagaaggt aaatcttcgg tttatttatc tgcatatcaa
      481 aaccgagaaa agacaaaaga taatcgaacc ggggcaacct gggattattc aaaaaaagag
      541 ggatttttcg gctctgctat cctctcccct gccggcacac ctgccgaact ggtgaaggac
      601 tcaggcaccc tctggaatgc cgtagaggct ggcgagaagc gaaaggatgc cgaattatgt
      661 cgctatgtgg acatagccat ccccaaggag ctggacgacg gccagaagaa gcagatcgtc
      721 ctcgattact gccaagaaaa tttcgtggat tatggaatga ttgcagacat agcatttcat
      781 gatcttgata gtcataaccc tcatgctcat gtaatgctaa ctttaagaac tgttagccca
      841 aatggatttg ggaacaaaca aagagaatgg aataatagag aaaatattga agcctggcga
      901 gttaactggg aaataatagc aaatgacaga ttaaaaaaat atggtcataa gtcccgtatt
      961 gattcccgtt cattaaaaga tcaaaaagaa gaggcagaaa gaaaggctgt ttttgctaaa
     1021 actccgaaag agaaagccaa gtggatttca aaaactattg aactggacag aacgccaatg
     1081 accaggatac acaggcacca atggcgaaaa ggtcaaaaat taagagctat ggagcaaaaa
     1141 gaaaaagcat tattattaaa aatagctaat gcaacatact tacagcaaac caaaccacct
     1201 cagccagagg aaactatgaa aatagagaaa aagcctcttg caaggtcaat cattgataat
     1261 ttaaaggcat tatacaaaaa gacccagcat atatttaaat caaaacctaa tgtaaataaa
     1321 aataaaaaag aagaaaaaga agatcctcaa aatttcgtta gtagttatgt aataaatgaa
     1381 tttggcgaac atatattaaa gacagagtta gaaacaaaac ctaaaacagg cacaggaacc
     1441 ggaaccggtg gaaataatgg aggcggaaaa atgggttcag gctctggaat gtctcctatg
     1501 aaccaaccac aaccaaaaat tgaagagcct gagccggaac caatgccgga attcgaagat
     1561 cagcaaccta agaggaagaa gaaatttgga atgtagtata tagtggtata tattcctata
     1621 tagtagtatc acttaataca acttaatatt atttaatact atactataat atactacatt
     1681 tttagagttg tgatcctcgc cggttctatt tcccctttat gtattgccac agcttcatta
     1741 actgacttca taagcatttt aaaaaattta ctatgcttat ttcttttccg ttttttctgc
     1801 actttctttt ttttcatata ttcaccaaaa aaaatcgaat cgccaatttt tcttcgaaca
     1861 atactctttt ttcttttttt attgtttttc tatcttcggt aatgcttcgc accatagatc
     1921 tgatagtaat gctttgctgc cgtatgtaaa gaactgcccc aacttcgttg ggctgttatc
     1981 tgatatggtt aagctcccca cttgacaaat ttttaagatc ggttagaact ggtacaggat
     2041 tgaaaaacct ataaaaataa aaaccccacc ctctgcgatt ttgttttggc gacagaaaac
     2101 agagagtagg gccagataaa aaggactgaa aaaatgataa tacaagaaga acataacatc
     2161 gattttttta aaaatcaaat gcctaacaag ccttattgta caaataacct cgatatggga
     2221 ttaagcataa ggaacaaggc aaaagcctta gaaatgctgt atttacaggc aaatcagcca
     2281 gcaatccaaa cttgcttact ctttgattta gataagaaaa actcattcta tacatttgag
     2341 aaggtgggct tacccatacc tcactttatt acaaaaacac caaagacagg tcgttgccat
     2401 tacgggtaca tgcttaaagc cggtgtttgt aagacacaac aagcaaggtt gaagccgttg
     2461 aagtatgccg ctgccgttga aatggctatg gcgaaaaaac tcaaagcaga tctggggttt
     2521 gctggattga ttacaaaaaa tccactcaat gatcactggt cgccgttctg gtcaggtgct
     2581 gacctttacg acctggatta tttagctgat tttgtggact tagaaaaacc tcaaatacaa
     2641 gagaaaaaaa gcgaggctta tggtttaggt cgcaatgtta atttgtttga agatctacga
     2701 caatatgctt acagaacggt tttaaatttc aaaaaaatat ctactttcga gaaattcgaa
     2761 aacgaactat tattaaaagc acagggttta aatacttttt gcaatccact aaatccgtta
     2821 caatacaaag agttaaaggc aacagcaaga agcatagccc gttggacctg gaaaaatttt
     2881 gatagccata cattttcact aatacaatct tcaagaggtg cgaaaaataa aaacaaaccc
     2941 aagaagagta atgttaaaga aatgcttggt tttataggag gtgaaaaatg agaagaagca
     3001 caataagaaa aggtttaact caaaaagaaa tggctgaaag atttaatata tctgttaaaa
     3061 cggtaaaaaa atatacatcc gaaccgcgtg aacaatacga acaaacagcg gctgatcgtc
     3121 gtcgtcaggc ttacgaacta cgaagccagg ggctgaaatg gcaacaggta gccgacaaaa
     3181 tgggctgttc gtatcacgga gccgttgccc tgtatcgccg ttatgttgcc ttagatatgc
     3241 ctaaaaacag cctgtaacga cttctaagca gtttttcccc ctaacctaac caaaccctcc
     3301 acccctgggg ggatttttcc ccctttcccc gtatccgggc ttgtgtctgg ataggtgagg
     3361 gacactctgg taccaacaaa tcaacaaacc cacccagcca ccggaggccc tctggcaacc
     3421 ttaaaacctc cagccgttgg ctggcaaccc ttcacagcaa aaaaccagcc caaaaagcgg
     3481 ctggttcttc actgttcacg gtttacgatt tccattgttg caatatatcc attccgattc
     3541 gtgtaaaatc tcttttctct tcttcgtcta actcattgat tttacttaac aagccaagaa
     3601 ttaaagcctt gttaatttcc tctggttttt tatctaacac cttagccact tctgcaccca
     3661 ggattatttt ttgtcttgtt tcttcccgtc tgtcctgcct tgcttttttt gttcttagtg
     3721 cttgtaattt attttgtaga tctgcgatct gctgtgctgt tgatttgctc atattacaac
     3781 ctccttttat ctttattatc tttgttatct tctacttgga ttatttcatt taatttatct
     3841 aattcataag aagccagttt ttgaacaatc acaaactgga aattctcata atcatataat
     3901 ggccattcaa gcaaccgcca aacaaaagga tcgtgatctc ccaaagggct taaatcatat
     3961 gttgtaattc caaaatctaa aataacagca gttctcagat ctattttatc tcgaaaaatt
     4021 attctacata tatcctcaac tggatactgg aaaaaatcca gagccatatt atttttattt
     4081 aaaaaagaca ctacctcttt atgtaaaatg tttaaacagt cgttaaactc ttgttcttca
     4141 aactctaaac ctctatattg catattaaaa acctccacac tat
//
