LOCUS       CP056479                4667 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00473 plasmid pRHBSTW-00473_6,
            complete sequence.
ACCESSION   CP056479
VERSION     CP056479.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148625
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00473
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 102.372331253518x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 01:41:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,902
            CDSs (total)                      :: 4,787
            Genes (coding)                    :: 4,649
            CDSs (with protein)               :: 4,649
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 49 of 138
            Pseudo Genes (incomplete)         :: 86 of 138
            Pseudo Genes (internal stop)      :: 20 of 138
            Pseudo Genes (multiple problems)  :: 15 of 138
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00473"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00473_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4167..4667,1..265)
                     /locus_tag="HV208_24460"
                     /pseudo=""
     CDS             join(4167..4667,1..265)
                     /locus_tag="HV208_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /name="cache domain-containing protein"
     gene            553..1056
                     /locus_tag="HV208_24465"
     CDS             553..1056
                     /locus_tag="HV208_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="QLU38094.1"
                     /translation="MERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDV
                     DFFKKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECV
                     AERIRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIV
                     YSAI"
                     /name="GGDEF domain-containing protein"
     gene            2116..2439
                     /locus_tag="HV208_24470"
     CDS             2116..2439
                     /locus_tag="HV208_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLU38093.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2378..3142
                     /locus_tag="HV208_24475"
                     /pseudo=""
     CDS             2378..3142
                     /locus_tag="HV208_24475"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3117..3602
                     /locus_tag="HV208_24480"
     CDS             3117..3602
                     /locus_tag="HV208_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLU38091.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3606..3821
                     /gene="mbeD"
                     /locus_tag="HV208_24485"
     CDS             3606..3821
                     /gene="mbeD"
                     /locus_tag="HV208_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLU38092.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="mbeD"
     Typing          1831..2003
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(1354..1906)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2117..2433
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          2430..3925
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tacatagggc atgtcggtgg gtctatatat ctaaaaaaac atagtttatt tagcgatgtt
       61 ttgagcaggc attttttcaa gagcgacact gaaattagca ttgttagtga tgatggaact
      121 gtgatcttta ataacgataa cagtgctgtt ggtcggccca tggttatgtc tgatgatttg
      181 aaatcaaagt taacggagtc tgatagaggt gagggggaat tttttttttc ggggaataga
      241 tatcttctgg gttatgctca tatgaaaaat actgcttgga atgtgttggt ttatagccat
      301 gctgacagcg cgactactat tcttattact tatgctaaaa gtgtacttgt tctgctaatt
      361 gtaataatta ttacattttc acttctttca tatttcattg cctcccgtat ttcacttcct
      421 ttagaaaagc ttgctatatc taccaatgct aaagatatag gggcttcgtt agtttatatt
      481 aaagctatca atgcttggta tgccgaagct gatcgactta aaaaggcact ttttacgcat
      541 gttaaggtaa tgatggaaag ggttgattct ttaaatgagg tggcgcttaa agatcccctt
      601 accggcgtga acaatagatt ggggttttca actaaaatac agaataacaa gtatggggca
      661 ggagcttcag ttattgctat tgatgttgat ttttttaaaa aaataaatga tagtttcggg
      721 catggggtcg gagatgaggt gcttgtttct ttggctcaga ttattaagtc ttgttgccgt
      781 agtgaagata ttatttgtcg gtttggtgga gaagagtttg ttgtttttct gccgaataca
      841 tctgtggtaa cagcggagtg tgttgcggag cgtattagga gtgttattga acgtactgtt
      901 ttccctaata acttgcgtgt caccgtatct gctggtgtgg caacacaaga tgatcctctt
      961 ggtgggatcg attttttgct gaaaaatgct gacgatgctc tttatcaagc taagagagag
     1021 gggcgtaata aagtgattgt atatagcgct atttgagttt ttttgatgac gctttacgcg
     1081 ctagtgtgcc gttttttttt gtggggaggg gggttcaaga agcggatcct cttgttatcc
     1141 cttcgatgtc gtcgtaatct catgccctgc aaacaaaaaa accaccgcta ccaacggtgg
     1201 ttcgtttgcc ggaatcaaga gctaccaact ctttcttccg aaggtaactg gctttcacgg
     1261 agcgcaggta ccaaaactgt ccttccagtg tagccgcagt caggccacca cttcaagaac
     1321 tctgtagaac actctactac tccgctccgt gaatcctctt actaatggct accgccactg
     1381 gcgatttttc gtgtctgttc cgggttggac tcaagatgat agttaccggg acaacgtggt
     1441 agtcgggctg aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcccgg
     1501 aaccgagtgt caggcgtgga atgagataaa cgcggccata acagcggatt gacaccggta
     1561 aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa cgcctggtat
     1621 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     1681 tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg ccttatcgct tccctgttaa
     1741 gcttcttcct ggcatctccc aggaaatttc cgccccgcct gtaagccggt accgctcgcc
     1801 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaatat atcttgtatc
     1861 acatattctg ctgacgctcc gctgctgcat ttttctcctg ccacatgaag catttcactg
     1921 acactcgcat ccgtgccaac atagtaagcc agtatacact ccgctagcgc tacgtgactg
     1981 gttcagggct gcgccccgaa acccgccaaa agcctgacgc gcctgcggct tgtccaaccc
     2041 cgcgccgacc gggaaaggct ttcccgcccg tcccggcgtt atcaggaggc cggtttgtca
     2101 gagaaacgga acaagatgct caccatgtgg gtgacggagg acgagcaccg gcggctgctt
     2161 gagcgctgcg acggcaggca gctggcagcg tggatgcggc agacctgcct ggacgaaaag
     2221 ccggcacgtt ccggaaagct tccctcaatc tcgccggcgc tgctgcgcca gcttgccggc
     2281 atgggcaata acctgaacca gatagcccgc cgtgttaacg ccggcggcgg taccggccat
     2341 gacagggtgc agattgttgc ggcgctgatg gccatcgatg ccggacttga gcggctgcgg
     2401 cacgccgttc ttgagaaagg aacggacgat gatcgttaaa tttcatccac gcggtcgcgg
     2461 cggtggcgcc gggccggtgg actatctgct gggtaaagac cggcagcgtg aaggcgccac
     2521 cgtcctgcag gggaagccgg aagaagtccg ggagctgatt gacgcgtcgc cttacgttaa
     2581 gaaatacacc tccggggtac tgtcgtttgc ggaagcggat ttaccctccg gccagcggga
     2641 aaagctgatg gcaagctttg agcgggttct gatgcccgga cttgataaag accagtacag
     2701 catcctgtgg gtggagcatc gtgacaaggg gcggcttgag ctgaattttc tgatcccgaa
     2761 cacggagctg ctgaccggca gacggctcca gccgtattac gatcgcgccg accgtccacg
     2821 catcgatgcc tggcagacca tcgtgaacgg cagactgggg ctgcacgacc cgaacgcacc
     2881 ggagaaccgg cgggcactgg tcacaccgtc tgcactgcct aaagcgaagc aggaggctgc
     2941 agaagcgatt acacgcggtc tgcttgctct tgcctcatcc ggggagctga aaacccgtca
     3001 ggatgtcact gaggcgctgg aaagcgcagg ttttgaggtg gtccgcacca caaaaagcag
     3061 tatcagcatt gccgacccgg acggggggcg aaacatccga ctgaagggag ccatttatga
     3121 acagtctttt aacgctggcg aaggacttag agcagaaatc gaaagcgcag cagcagagta
     3181 ccggcgagat gctgaaagcc gcattcagcg agcacgagaa atctgtaaaa gcggaactga
     3241 acgaaagcgc gaagagaatc agcgccgcca tcctcgacca cgaccggaaa ctgtcctcag
     3301 ccatgagcca gcgcacgaaa ggcatggtgc gcatggtcag ccagacgtgg ctgaccatcg
     3361 tcctggtctc cgcgctgctg atcgcctcga gcgcgggcat tctgtggtgg caggggcagc
     3421 agatactcga caactacacg accatccggg agcagaagag cacgcaggcc atgctgtcag
     3481 agaggaacag cggcgtgcag ctctcgacct gcggcgagca gagacgccgc tgcgtgaagg
     3541 tgaacccgga agcgggacgg ttcggagagg attcgagctg gatgatactg gcggggaaat
     3601 agcacatgac ggagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact
     3661 actcgaaaag gctggacgag tgggagagcg ccttcgcgga atggcggacg atgtctggtc
     3721 ttatgcaacg ggagaacgca gcgctgagcg agcgcgtcac gggcttgagc aggcaggtgc
     3781 agagtttgag cgagcagctg cgccggttgt cgagcgactg aatgccgtcg agctgcaccg
     3841 ggagcaggag cgcgtggtgc agcatcagaa agcgctggag ctggagcgat ctcagcgaca
     3901 gcggacgtat aacagccctt cactgtgata acctgataaa acggggcttt cggtggacat
     3961 ctgcgctgaa agccggagcc attcacccga ctgcatagcc atgcagccgg gtgaaattta
     4021 tctggcgcag aatgcgcggt ttagcggaga gtttgctgcc ggttttaccg ggctgacgcc
     4081 gcatttgttc cgaacccgtc cggggcggtg ctgataaggg atgttatggt aaatgcggca
     4141 ttttttatta tatggagtgt atttttatga gaattaaaaa gcctcacctt cgtccgatag
     4201 tcataacctt gtccgttggt gggattttgt taacttctat ttttctgatt gccgttatta
     4261 tggtttttca gcgcgaaaat atagaaaaag atttgcttga tgctaattca tcgtacgcta
     4321 tgaaaatgtc agatgtaatg agtggttata ttgaaatggc tcaaggtgaa ttagcatatg
     4381 gagcaaaaaa aataaaatca actaatgata ttgactcact taaagatgag gcggataggt
     4441 taagattgca gtctggtatg tttaattcag tttttgttgt tagtaatgat gctgttgttc
     4501 tggcgacttc gccagaatct cttgatttag ttggtacccg cttgaattca agtgccagca
     4561 aattggcgat tgaggaaaag aaaccattca tatcttcacc gttcaaatca gttactggga
     4621 atttgattat tttgttgtca catcctattt atgataagaa tggcaat
//
