LOCUS       CP056478                4921 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00473 plasmid pRHBSTW-00473_5,
            complete sequence.
ACCESSION   CP056478
VERSION     CP056478.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148625
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00473
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 102.372331253518x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 01:41:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,902
            CDSs (total)                      :: 4,787
            Genes (coding)                    :: 4,649
            CDSs (with protein)               :: 4,649
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 49 of 138
            Pseudo Genes (incomplete)         :: 86 of 138
            Pseudo Genes (internal stop)      :: 20 of 138
            Pseudo Genes (multiple problems)  :: 15 of 138
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00473"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00473_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(9..224)
                     /gene="mbeD"
                     /locus_tag="HV208_24435"
     CDS             complement(9..224)
                     /gene="mbeD"
                     /locus_tag="HV208_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLU38087.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="mbeD"
     gene            complement(231..722)
                     /locus_tag="HV208_24440"
     CDS             complement(231..722)
                     /locus_tag="HV208_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLU38088.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(697..1242)
                     /locus_tag="HV208_24445"
                     /pseudo=""
     CDS             complement(697..1242)
                     /locus_tag="HV208_24445"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1400..1723)
                     /locus_tag="HV208_24450"
     CDS             complement(1400..1723)
                     /locus_tag="HV208_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLU38090.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2730..4034)
                     /locus_tag="HV208_24455"
     CDS             complement(2730..4034)
                     /locus_tag="HV208_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013357653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="QLU38089.1"
                     /translation="MFSLKNMFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFL
                     STMGYRADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLE
                     ASYIPVSDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEAN
                     ENFVNATGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDP
                     LWFRATYNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGA
                     NVIENSILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEA
                     ARAGASGRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREG
                     VTLMREAGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     Typing          complement(1837..2009)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          1934..2480
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2..1410)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.857"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatggcattc agttcccgga caagcggcgc agctgctcgc ttaaactcag cacctgcgtg
       61 ctcaagtccg acacgcgctc gttcagcacc gcgttctccc gttgcataag accacacatg
      121 ctccgccatt ccgcgaaggc gctctcccat tcgtccagcc tttgcgagta gtcctgctgt
      181 agctgctcta atacgctcag caactgcttt tccagctctg tcattttccg ctactcctgt
      241 ttcaggcgga tccatgtcgt cccgtcgtag gggatggctt cggcttgcat ccccggcggc
      301 aggataagaa acctccggcc gtcgctgctc tcctgatagc gcactcccca cgtccgggcg
      361 gtcattttcg ccagggtatc ttcctgctga cgaagatgcg tgtaattgtc ggttatctgc
      421 tgcccctgcc accacagaat gctcccgctc gtggcgatca gcagcgctga caccatcagg
      481 atggtgagcc atgtcctccc ggccgtcagc aacacgctcc gccggttttt ctccatcgcc
      541 tcgctcatac tctgctcgtg ggcgctgatg gcgtcgctga ttctcctcgc gcttgcgttc
      601 agttccgctc tgacagactg ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta
      661 ctcttctgct gcgctttcga ttgctgctct aagtccttcg ccagcgttaa aagactgctc
      721 atagatggct cccttcagtc ggatgtttcg ccccccgtca gggtcggcaa tgctgatact
      781 gcttttcgtt gtacgcacca cctcaaaacc tgcaccttca agagcctcag tgacgtcctg
      841 acggcttttc agctccccgg acgaggctaa gctcagcagt ccacgggtga tggcttctgc
      901 ggcttcctgc ttcgttttcg gcagtccggc cggcgtgacc agcgcccggc ggttttccgg
      961 cgcgttcgga tcgtgcagcc ccagtctgcc gttcacgatg gtctgccagg catcgatacg
     1021 cggccggtcg gcgcggtcgt agtacggctg gagccgcctg ccggtcagca gctcggtatt
     1081 ggggatcaga aagttcagct ccagacgccc cttgtcctcg tgctccaccc acagaatgct
     1141 gtactgatct ttatcaagtc cgggcatcag aacccgctcg aagctggcca tcagtttttc
     1201 ccgctggccg ggaggcagat cggcctccgc aaacgacagg acgccggagg tgtatttctt
     1261 cacgtaaggc gacgcgtcaa tcagctcccg gacttcttcc ggcttcccct gcaggacgct
     1321 ggcgccttca cgctgccggt ctttacccag cagatagtcc accggcccgg cgccaccgcc
     1381 gcggccacgt ggatgaaatt taacgatcat cgtccgttcc tttctcaaga acggcgtgcc
     1441 gcagccgctc aagcccggca tcgatggcca tcagcgccgc cacaatctgc accctgtcat
     1501 ggccggtgcc gccgccggcg ttaacacggc gggctatctg gttcaggtta ttccccatgc
     1561 cggcaagctg gcgcagcagc gccggcgaga ttgaggggag ctttccggtg cgcgcaggtt
     1621 tttcgtccag gcaggtctgc cgcatccacg ccgccagctg cctgccgtca cagcgctcaa
     1681 gcagccgccg gtgctcgtcc tccgtcaccc acatggtgag catcttgttc cgtttctctg
     1741 acaaaccggc ctcctgataa cgccgggacg ggcgggaaag cctttcccgg tcggcgcggg
     1801 gttggacaag ccgcaggcgc gtcaggcttt tggcgggttt cggggcgcag ccctgaacca
     1861 gtcacgtagc gctagcggag tgtatactgg cttactatgt tggcacggat gcgagtgtca
     1921 gtgaaatgct tcatgtggca ggagaaaaat gcagcagcgg agcgtcagca gaatatgtga
     1981 tacaagatat attccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2041 cgagcggtac cggcttacag gcggggcgga aatttcctgg gagatgccag gaagaagctt
     2101 aacagggaag cgataaggcc acggcgaagc cgtttttcca taggctccgc ccccctgaca
     2161 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     2221 accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     2281 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     2341 gggaaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     2401 tgcgccttat ccggtaacta tcaacttgag tccaatccgg aaaaacacga caaagcgcca
     2461 ctggcagcag ccactggtaa caggtttaga ggaggagagt cttgaagttt cgggtgcgat
     2521 aaggctacac tgaaaggaca gttttggtga ctgtgctcct ccaagccagt taccgcggtt
     2581 caaaagagcg tcaactcttg cgaaccttcg aaaaagccac cttgccgggt ggttttttcg
     2641 ttttcagagc aagagattac gacgcaacgg aaaggatctc aagaagatca tctgtgatgt
     2701 tcgacagata tggggatatg caccatggcc tatccggctt taactgaaat ggcgacatcc
     2761 ttaaccgcag tctcaacccc tgcagcattc ctctgaatgc tggtcataac ttcacccgct
     2821 tcgcgcatga gtgtaacccc ttcccgagtg ttcatcaggc tgttttcaat gccacagaga
     2881 acatccttgg ccagttgact attgctggcc acgacttgct caatttcttc agtcgcgcta
     2941 ctgacgctcg ccgcaagatt acgcacttct gcggcaacaa ccgcaaaact tcttccggat
     3001 gcgccagccc ttgctgcttc aatcgcggca ttcagtgcaa taagtcttgt ctgcatggca
     3061 aacttccgga tggtttccac catatcgtca atactttcag actgattgtt gagccgtgag
     3121 atatcggtgg agacagcccc catcccctgc gcaattctgt caatcataag gatgctattt
     3181 tcaataacat tggcaccgtt ctgcgcactt tctcgggtct gcacggccat atcccatgca
     3241 tgcacagcag cctcttgctc tctctggttt cgcaggacat cagccgtaac atctgtagcg
     3301 aatttcacaa ttttataaag ctgcccatca ctgttaaaga cagggttata agttgcacgg
     3361 aaccacagag ggtctccctg acggttaagg cgtggaaaca gaccagaaaa aaactcacct
     3421 tgattcagac tctcccagaa atgtcggtat tcatcacttt tgtaaagcgt ctcggcacag
     3481 aacagacgat gatgcttacc tatgatttca tcccgcttat agccagtggc attgacgaaa
     3541 ttttcatttg cttcaagcac gatcccttca ggattgaagg tgatgatggc catagaacgg
     3601 tttatggcat ttacaactgc ttcctgctca agagcagaat gcacgcgctc agaaatatcg
     3661 gcagcaattt taatgactct gataacacgg ccgcgcctgt cagaaacggg gatataactg
     3721 gcttcaagcc atacggaacg atttcccttt gccagccgca ggtacttgcc actaaagctt
     3781 tctcccctgg caaggcgctg ccagaactgg ctgtattccg gtgaacttac cagcgagggc
     3841 gggcagaata tacgatggtg gtgaccgatt atttcatcag ccctgtaccc catagtggag
     3901 agaaaaagcg ggctggcttt atttatcacc ccctccggac tgaactcaat gacaggctta
     3961 ttctgacaaa ttgcgtcata cgaaatggct gatcgctgga ggttcaggga gaacatattt
     4021 ttaagtgaaa acatatgaat cagattgccc ggataatgta agtactgcca tggttatcgg
     4081 ctactcctta tgtagcgtta tatattactg cgacagggcg ggaaaaagcc cgctattcag
     4141 ccatgccggc atgatgtccg gctccggatt tcacgcgtaa aaaacccgcc atctttagcg
     4201 ggctgaacaa taagaaaatg ttcatattta cggcaaatcc gccggctcca gcagagaagc
     4261 tggcgtctat caatcttgga agaaccagcg cagctccgca tgcattaaca ggcatcagaa
     4321 ggcaaaaaaa tggctcccat ccgggagccg gaaaacactc aactgcctgg atcgcagcac
     4381 atcctttgtg aaggataatt taagtatcgg ccggattgcc gcgggcataa ttttttccgt
     4441 atccgccaga cctcggcttt taacataaca ccccttgccc gcaccgcaga aacggggttg
     4501 gctgcgtttc cggtgccaga cgcgagaaag tggcgacaga catgacgaaa aaacaccaaa
     4561 aacgcagccg ctggatatta cactgatgca tagtcatgca gccagatgca tgcagcgtaa
     4621 agtcacacta aaatcagcgc gaacgcctta aaaaccggca aatgcctctc cggcaactca
     4681 cacagctcca tcaccaggta aaaacagttc agcagggctt caataacgtt gagaattttc
     4741 atcacttcct ccgggataag tcactaccat tccggtagtc agcctattta gcaggaacgc
     4801 gatgagccct tcttcgtgcg ggctgtcacc tcatcttcgg accatcgaaa ctcttctgct
     4861 tctgtcggag cgtcagctcc agctcgttct gacgctgata ctccagtgca gccttctcct
     4921 c
//
