LOCUS       CP056477                8189 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00473 plasmid pRHBSTW-00473_4,
            complete sequence.
ACCESSION   CP056477
VERSION     CP056477.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148625
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00473
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 102.372331253518x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 01:41:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,902
            CDSs (total)                      :: 4,787
            Genes (coding)                    :: 4,649
            CDSs (with protein)               :: 4,649
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 49 of 138
            Pseudo Genes (incomplete)         :: 86 of 138
            Pseudo Genes (internal stop)      :: 20 of 138
            Pseudo Genes (multiple problems)  :: 15 of 138
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00473"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00473_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(8005..8189,1..28))
                     /locus_tag="HV208_24390"
     CDS             complement(join(8005..8189,1..28))
                     /locus_tag="HV208_24390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU38079.1"
                     /translation="MSNIKHDSLIRITKRKNENGDETTDLEWKPGSKEAIATGAAAGAA
                     LGSVVPVIGTAVGAAIGGTLALIFG"
                     /name="hypothetical protein"
     gene            complement(176..3073)
                     /locus_tag="HV208_24395"
     CDS             complement(176..3073)
                     /locus_tag="HV208_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="QLU38080.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            3168..3773
                     /locus_tag="HV208_24400"
     CDS             3168..3773
                     /locus_tag="HV208_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QLU38081.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            complement(3862..4305)
                     /locus_tag="HV208_24405"
     CDS             complement(3862..4305)
                     /locus_tag="HV208_24405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU38082.1"
                     /translation="MTSGIFLSREDLMLLSDSAKDEVLALVTGESISGVYEESEDGPSD
                     LSSLQAERLVRGLSDKSRSVLKAILESEDGELGFWCKSVAEELGFDVSELSGVWSGLTR
                     RTRTVSDDSDAYLIGWAWDEGRDDYYGKLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            complement(5377..5763)
                     /locus_tag="HV208_24410"
     CDS             complement(5377..5763)
                     /locus_tag="HV208_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLU38083.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKTGELSTCYGDRNARMVDVSE
                     LIRVFGTLEQRLVEQVSGQVNEQTDTGVSAQSEQVIAQLRQEVEHLKTLVSAKDSHIDS
                     LKQAMLLIEHRHTGDSSWWKFWKR"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5763..5954)
                     /locus_tag="HV208_24415"
     CDS             complement(5763..5954)
                     /locus_tag="HV208_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLU38084.1"
                     /translation="MTKQEKTALNMAKFIQGQSLLLLEKLNELDLDSEADMCEKLHEDA
                     ELLCSTLSGRLHALQDAG"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6787..7083
                     /locus_tag="HV208_24420"
     CDS             6787..7083
                     /locus_tag="HV208_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="QLU38085.1"
                     /translation="MSSMVYCRGCGKEIHETAKACPHCGATNVAVGQKSRIAAALLAFF
                     LGGFGVHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDENFSRKYG"
                     /name="NINE protein"
     gene            7141..7458
                     /locus_tag="HV208_24425"
     CDS             7141..7458
                     /locus_tag="HV208_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU38086.1"
                     /translation="MSVTCPKCGSDKTQKRNIGTKVASGIGAAAGGIGAATGGGEGAAV
                     GAAIGSALLPGVGTIAGGVIGFLGGLVAGATVGAAAGKVVDEYVIDTYKCNDCDNTFSN
                     N"
                     /name="hypothetical protein"
     gene            7483..7806
                     /locus_tag="HV208_24430"
     CDS             7483..7806
                     /locus_tag="HV208_24430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU38078.1"
                     /translation="MGNTKSSTIAGATGALATAGTAATTASAVVGGSAATIMSATAGTT
                     GAALASVAGASGTAGAAAISSGMAAIGSTVGGGMAAGAIITAAAPVAIGAAVVYGLFKL
                     FED"
                     /name="hypothetical protein"
     Typing          complement(4793..4874)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.524"
                     /coverage="92.0"
                     /evalue="2.56e-13"
     Typing          6114..6493
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.717"
                     /coverage="88.0"
                     /evalue="2.28e-160"
ORIGIN
        1 tcagactatc atgtttaata ttgctcattc gcacctcacg taagattgaa acgtatacac
       61 caaaaacact ctccctccac ctaggctaca tttcaactac atatattaaa aaccctgcat
      121 ttaccataac gtctcactat gtggggtcgg ttccggctga gggcgaaatg acaccctaag
      181 ctttcggttc cttgggccaa agatattcgc cagtcagtag aatgtgcgcc cagcccaatg
      241 gggatatgtg gggaagaaat tcagggggaa catccaaccc ttcgttccgc cgctccgtga
      301 cggcatgacc aagatggacg gtattccagt aaatgatcac cgcagtcaat aaattgagcc
      361 cagcgattcg gtagtgctgc ccctctgtcg tgcgatcgcg aatttccccc tgcctcccga
      421 tacggagcgc atttttgagc gcatggtggg cctctccctt gttaagaccg atctgagcac
      481 gccgctgcat gtccgtatcc aggatccact caataatgaa aagggtccgt tcaatacgac
      541 caacttcacg aagcgcaact gcaaggttgt tttgtcgtgg gtaagaagcg agcttgcgca
      601 ggagttggct gggcctgatt ttgccagcgg tcatcgtcgc ggcacaacgg aaaatatcag
      661 gccagttcgc aacgataaga tcctcccggg cttttccacc taccaacttg cgtaactccc
      721 tgggggtcgt atcgggatta aatacgtaca accgcttcga tggcagatcc ctgattcgca
      781 gaacgagatt gtagccgagc aggctactgg ctccgaacaa atggtcggtg aatcctgctg
      841 tatcggcata ctgttcgcga acatggcgac cgacctcgtt catcagtagt ccatcgagaa
      901 tatacggtgc ctcgctcacg gtcgccggga tcgactgaca agcgaatggc gcgaactggt
      961 cgcttacgtg agtatacgct ttgaggccgg gaacagaacc atatttggca ttgaccatgt
     1021 tcatggcttc gccatgccgc gctgtcggga aaaactgacc atcgctcgat gctgacgtgc
     1081 ccatccccca gacgcgtgac atcggcagtt taccctgcgc ggccaccaca attgccaatg
     1141 cctggttcat ggcttcgctt tcaacatgcc agcgggcaag gcgtgagagc tgccagtaat
     1201 catgcgtgtt tgtagcttcc gccatcttac gcaggcccag attgagccct tcagcgagca
     1261 ggacgttgag cagaccgatc cggtcgcgac atggagcccc ggttctcaga tgggtaaacg
     1321 catctgtgaa accaagggct gcatcaactt caagcagcat gtcggtaatc cgaacggacg
     1381 gcattcggcg atacagatcc agtatgagtg cctcggcacc atccggcacg tctgctgtca
     1441 acctgtcgat ccgcaacgtt ccatcttcta tgctaccgtg cggaatagtg ccgttacggg
     1501 cagcccgggc cagccgctta agagcgatcg tgagtcgcgc ctttctgtct gccagccaat
     1561 cctgtgggtt ggaaggcacg gccagttttg cattttcctg cgccgcgatc atcggcacca
     1621 gtacctgctt gaggtcacca tagcggcgcg aatgagcgag ccagacatct ccggaacgaa
     1681 aagcatcccg gaggtgaaag agtaccgcca cttcccaaag acgggtatct cctttttcct
     1741 gagctcgtaa atgacggttc catttggagc tgggccgcag gaaacgcctt tctggcgatg
     1801 caacaccttt catctctccg atcgacaaag ctgctgctac caatggtccg gcgaccggcg
     1861 cggcttcgag cttcagacag cgcaacatgc ggggcgcata acgacgaaag cgatggtatc
     1921 cctgcccgac atatgcaaga ggctcatcgg ctagcgtgtt gctgagttga gtccctgtcg
     1981 ctaccagttg agcgagccgg tcccatgcaa ccgaactggc gacagccatc tccagcgggg
     2041 ttccgtcact gcgggcctca agcaacgaag ctcccagcgc ggtgaaggta cggatcgtat
     2101 ccgtgagtgt ggctttagag ccggaaattg tttcgtcatg ctggcgcttc gcttcccgcc
     2161 aggtttttcc tacgatcctg tcatgggttt cgactatggc atcagcaatc gccgcttccc
     2221 actccacaac acagacggca aggatcgccc agcggcggtc cgaagtgatg tcacgcaaac
     2281 cgtcggtgaa gtagcgttca ccctgccgac gcagccgggc aatgcgatgg gcaggtatgc
     2341 tggccaaagc actatgattg atattcaggg tacgcagaaa ttcgagcctg tcgagcaaac
     2401 ggttagcagc agccgagttg ttaccaacct cgaagttgcg aagccagatg aaacgactga
     2461 tattgccggc gagcatttca ctcagaagtt tgtccaggtg atcgcgaaca tccgctgtta
     2521 aattttccgc aatccgcgtt tcaatccgcc gctcagcggc gaccagagca tccgcgcaca
     2581 agcgctcgat tgtcgatact gcgggcagaa tggtggaagt ttcccgacac cgcacaataa
     2641 aacgatgagc aagatcctcg tttgatctgg catcttcggc ctggccgaaa gtccactccc
     2701 gcagatcacg ggcaccacgg cccgtgaagg tcttgtagcc gtaaatttcg cgcagcgtgt
     2761 ccatgtgctg ctgacgggtt tggcgccgtg tggcataagt gagaagcgca tcagccggaa
     2821 ctccaagctg agcaccgacg aaggaaagga cttcacgcgg gatcatctca ccaggagcca
     2881 gtgcacggcc cggatatcgt aaggcacaaa gttgcagggc aaagccaatc ctgttttccg
     2941 gtctgcggcg ctgcctaatg ttttccaggt catcatcgcc cagcgtgtag aacttcagta
     3001 gcgacagttc gtccgtgggc agatcgaaca gcgctgctcg ctgccgttcg gtgaaaatat
     3061 ggcgtcgtga catacaaatt cgtccctttt gaagtatagt ctgttttgga caacagccag
     3121 cccatataaa tcagggcgtt ccgatacaaa aatccaggag ggttcaattg ggacatcgtg
     3181 ccgccattta ctgccgggtt tcaacagcgg atcagtcttg tgaacgccag gaatttgatc
     3241 tgcgagcctt cgccggccgt gccggctacg acgtggtggg aatatttaag gaaacaggtt
     3301 caggaactaa actcgaccgg gccgagcgaa agaaagtcct ggcgcttgcc cagtccagac
     3361 aaattgatgc aatcctggtc actgagcttt cccggtgggg gcgctcgacg ctcgatctgc
     3421 tcaatacgct acgtgaactg gagaactgga aggtttccgt gatagccatg aatggaatgg
     3481 cgttcgatct ttcgtcgccg tatggacgaa tgctggcgac gtttctttcc ggcattgcgg
     3541 agtttgagcg ggatctcatc agcgagcggg tcaagtcagg ccttgctgtt gcgaaggcac
     3601 gtggtaagag gcttggtcgt caggccggag tgcgaccaaa atcagaccga cttttgccta
     3661 aggtggttgc gatgagggcc gagggacgca gctatcgctg gatcgcacgc gagctcggta
     3721 tcagcaagaa taccgtcgct gacatcgtgc aacgacacag agctaacgct tagggtgtca
     3781 tttcgccctc agccggaacc gacccctatg tgcacaaaat gaaaaagccc gcacatggcg
     3841 ggctcaccgg gcgaaaaaca atcaaaaatt cattgctttc ttgcagttgc gataggtggt
     3901 tggatgcaac ttcccgtagt aatcatcacg accttcatcc caggcccagc caatcaaata
     3961 ggcatcggaa tcatcgctta ctgtacgtgt tctgcgagtc agtcctgacc agaccccaga
     4021 aagctcagag acatcaaagc caagctcctc tgcaacgctc ttgcaccaga atcccagctc
     4081 cccgtcttca ctttcaagaa tggctttcag cactgaccgt gacttgtcac ttaaaccacg
     4141 aacaagcctt tcggcctgaa gagaggaaag atcactaggg ccatcctctg attcttcata
     4201 cacaccagaa atagactccc cagttaccaa tgccagcact tcgtctttgg ctgaatctga
     4261 taacaacatc aaatcttcac gggataaaaa aataccacta gtcatgaaag actccgtcac
     4321 cttgttaaga tgaaagaaca ataaacgcag acaagacact tgtcaataca acaagcatca
     4381 agtcttatag actaagcaag ttaatgtatt gtagggtaaa gataagggat accagagtta
     4441 cggaagagaa cgatagacat tcgacagctc ggatttacca taacgtcgca ttacgcgcac
     4501 cgctcaggcg gcctgagcgc gtttctgacg gcagaacggt aaaacagaca acaaaccacc
     4561 cgaaaagccg ccagaaacgc aggggatgaa ttctggcaca gtgaatggct atgcagccag
     4621 gtgaatggct ctgattccgg cgccggatcc accccataac ctctcttttt cacactttct
     4681 gggtatgccg gcgtgcattt ctggcaggtg attttgcgca tgcgcaaaat ccggctgcgg
     4741 acaggtagtg gtgaacgggc gcgtaattca ttgcgtggcc gtgaagcact tgccgggtgt
     4801 cggggcggcg agcccagacg ggagcatttg gcgtgccgcg tgtgcgcggt attacaaatg
     4861 cacatcctgt cccgtggcgc tgataccctc agaaaaattg aagccgcgcc cccaccctga
     4921 aaatgccggc aagccacagt gccggatcca gcaccacact ggatataaat cagtaacaca
     4981 atacggccac ctggatgaat atcatgtagt cggatccggc actgtgcggc gttatacagg
     5041 tatgtggatc cggcaccagt gccggattgt gtgacatcca actaactgat aaataaagat
     5101 agccacaaac cggcgatatg acagtgccgc ctcctgcacc aacaggtgtg tggattgtgt
     5161 gacattttcc gggatatatg taaatcgcct gaaatatatg gacttatact actgataagg
     5221 aaggataaat ctgcaagacg cagtcttgcg tggggtgtga tgttttgacc ctgaaggaag
     5281 agagcggcga gcgctgtgtc cggaccggcc gaaaaaacag gtttggatta agtcattcag
     5341 taaatcagac aagccccgcc ggcaactgag gcgttctcag cgtttccaga acttccacca
     5401 ggatgaatcc ccggtatgcc tgtgctcgat cagcagcatg gcttgtttga ggctgtcgat
     5461 atgtgaatcc ttggcggaaa ctagcgtttt gaggtgttcc acttcctgtc gcagctgtgc
     5521 aatgacctgt tcactctgcg cggaaacacc tgtgtcagtc tgttcattga cctgtccaga
     5581 aacctgttca accagacgtt gctcaagggt tccgaaaaca cggataagct cagaaacatc
     5641 gaccatacgg gcatttctgt ccccgtaaca agtggacagt tcacctgtct tgatcaacct
     5701 gtgcagtgtt gtccggctct ttccggtcag tcgtgcggcc tcactgatcg atacttttgc
     5761 cattaacccg catcctgtag tgcatgtaac cgcccggaga gagtactgca gagtagctct
     5821 gcatcctcat gaagtttttc acacatgtcc gcctcactgt cgagatcaag ctcattgagt
     5881 ttctcaagaa gcaggaggga ctgtccctgg atgaattttg ccatattcag cgcggttttc
     5941 tcttgtttcg tcatgagcag gcctcagtgc gtacgtgctt aatgtggaga cagactacat
     6001 tctgcttcct cgcccgttcg ctcgcaatgc tcggtcacgg gtctgcggcg agcaggacgg
     6061 actcgcgaag ttacccaaag attaagggga tacctgccgg gaaagaaaga cgaaaaagat
     6121 aaggccgcag caaagctgtt ttccataggc tccgcccccc tgacaagcat cacagaatct
     6181 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6241 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     6301 tgttatggcc gcgtttatct cattccacgc ctgacactca gttccgggaa ggcagttcgc
     6361 tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc cttatccggt
     6421 aactatcatc ttgagtccaa cccggaaagg cacgacttat cgccactggc agcagccact
     6481 ggtaactgat ttagaggagt tagtcttgaa gttgtgcgcc tgttaaggct aaactgaaag
     6541 aacagatttg gttactgcgc tcctcataag ccagttacca cggttcaaag agcgccaact
     6601 cttagaacct tcaaaaaaac cgcccgccaa ggcggttttt tcgtttacag ggcatgagat
     6661 tacgactcta ttgaaaggat ctcaagaaga tcctttcagc cccccccctc caagcatata
     6721 ggatgcactt tgttgtcaaa aatcggatat catttccttc aaataaaaat gtattagagg
     6781 aaaagaatga gctctatggt ttactgccgc ggttgcggta aagaaataca cgaaacagca
     6841 aaagcatgcc cgcattgtgg agccacaaac gtagcagtag ggcaaaaaag tagaattgca
     6901 gcggctctac ttgcattctt ccttgggggg tttggtgtac ataaatttta tcttggaaaa
     6961 attgggcagg ggtttcttta tttaattttt tgttggacct tcataccagc gattattgct
     7021 ttcattgaat ttattattta cttatgcgat tcagatgaaa acttttcacg caagtacgga
     7081 tgaagtgaag actaaaacag cccccaaagt cacactaaag ctaaattaaa agagaatgcc
     7141 atgagcgtta catgtccaaa atgtggttcg gataaaacac agaaaagaaa tattggcaca
     7201 aaggtcgcga gtggaatagg agcagccgca ggaggaattg gagctgcaac tggtggtggt
     7261 gaaggcgcag cagtcggtgc agcaatagga tcagctttac tccctggcgt aggaaccatc
     7321 gctggaggtg ttattggttt tctaggtggg ctagtagctg gcgcaaccgt tggtgccgca
     7381 gcagggaaag tggtagatga gtatgttata gatacgtata agtgcaacga ctgcgacaat
     7441 acatttagta ataactgatc accgttttaa aaagggataa aaatgggtaa cacaaaatca
     7501 tccacaatcg ctggagcaac cggagcattg gcaacagccg gaacagcagc gacaacagcc
     7561 tctgcagttg tcggaggatc agcagcaaca ataatgtcag ctacagcggg aactacagga
     7621 gcagctttag catcagttgc aggagcatca ggtacagctg gtgcagcagc gatctcatcg
     7681 ggaatggcag ctattggttc tactgttggt ggcgggatgg cggcaggtgc aataattaca
     7741 gcagcagctc ccgttgctat cggggctgca gtagtttatg gcctatttaa attatttgaa
     7801 gattagacca ttaagttaca tggtagatga gcgtagccgc tccattactt caagacataa
     7861 aaaccacctt taccaggtgg ttttttatgt ctccggcaca gactgattta ccataacgct
     7921 tcattgctcg catgagcgta aaaaagcccg cttttagcag gctaccttcc gccattcctg
     7981 acgggtgttt cgctctaaac ttggttatcc aaaaattaaa gctaaagtcc caccgatcgc
     8041 agccccgact gcagttccta ttacaggaac aacagaacct agtgctgctc cagcagccgc
     8101 gcctgtggca attgcctctt ttgaaccagg cttccattct aaatctgttg tttcatcgcc
     8161 attttcattc tttctttttg taatccgga
//
