LOCUS       CP056465                4858 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00490 plasmid pRHBSTW-00490_6,
            complete sequence.
ACCESSION   CP056465
VERSION     CP056465.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148634
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00490
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.45804921558x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 01:33:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,086
            Genes (coding)                    :: 4,983
            CDSs (with protein)               :: 4,983
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 32 of 103
            Pseudo Genes (incomplete)         :: 60 of 103
            Pseudo Genes (internal stop)      :: 21 of 103
            Pseudo Genes (multiple problems)  :: 10 of 103
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00490"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00490_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4517..4858,1..150)
                     /locus_tag="HV217_25995"
     CDS             join(4517..4858,1..150)
                     /locus_tag="HV217_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLU74811.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            157..372
                     /gene="mbeD"
                     /locus_tag="HV217_26000"
     CDS             157..372
                     /gene="mbeD"
                     /locus_tag="HV217_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLU74812.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="mbeD"
     gene            844..1335
                     /locus_tag="HV217_26005"
     CDS             844..1335
                     /locus_tag="HV217_26005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU74813.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(1392..1820)
                     /locus_tag="HV217_26010"
     CDS             complement(1392..1820)
                     /locus_tag="HV217_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLU74814.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            2105..2533
                     /locus_tag="HV217_26015"
     CDS             2105..2533
                     /locus_tag="HV217_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU74815.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            3516..3839
                     /locus_tag="HV217_26020"
     CDS             3516..3839
                     /locus_tag="HV217_26020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLU74816.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3230..3403
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(2761..3306)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          4..464
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="99.0"
                     /evalue="1.66e-87"
     Typing          3830..4857
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.21"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gataccctgg cgaaaatgac cgcccggacg tggggagtgc gctatcagga gagcagcgac
       61 ggccggaggt ttcttatcct gccgccgggg atgcaaaccg aagccatccc ctacgacggg
      121 acgacatgga tccgcctgaa acaggagtag cggaaaatga cagagctgga aaagcagttg
      181 ctgagcgcat tagagcagct acagcaggac tactcgcaaa ggctggacga atgggagagc
      241 gccttcgcgg aatggcggag catgtgtggt cttatgcaac gggagaacgc gatgctgaac
      301 gagcgcgtgt cggacttgag cacgcaggtg ctgagtttaa gcgagcagct gcgccgcttg
      361 tccgggaact gaatgccatc gagctccggc agagggagaa tgagtatcag aaaacactgg
      421 agctggagcg ccagccgcag aaaacctacc gcgggccgac actttaaacc atccaggcag
      481 ctgacaaccc gcacgaagag gggctcttat ttctcccgct aaataggttc gtgatcaaaa
      541 aggtcacgac ttattcagga gcgggaaatg aagctgttaa atcttattga atcattgctt
      601 aactgcctgt atctggcggc ggagctgtgg gactggctgg agaagcattt gtcggttttt
      661 aaggcattgg cgcggatttt agtgtgactt taacatccat gcatctggct gcatgactat
      721 gcaccagtgt aatattcagc ggctgcgttt ctggtgtttt ttcgttatgt ctgtcgccac
      781 tttctcgcgt ctggcaccgg aaacgcagcc aaccccgttt cagcggtgcg ggcaacagat
      841 gttatggtaa atcaaccgtc cgttgataaa ggttctgata atacccatgt aggtgatgtt
      901 gtttgctttt tatctacaca ctgcctgatg gagctattta tgaatgctga tgaagttaca
      961 cgaattcttg cgcaatcgag tcaaaatgat tggattgtgg atgatgagac aggggcgttt
     1021 acttataaaa atgatttagc actgcacatt aagagagctg attacgacga ctacaactcg
     1081 tttaatgagc cttgggctgt tcgtcatcct gatagtaatg cgtgttcagt cgagtacacc
     1141 gttcactatg gttcttcgtt tgttgataga aaaaccttgg tttccgtcga tggtcatcgt
     1201 gcaaccttgc ctatgcctgc atccgcaacg gatctaaacg tgtcatcggc tgatgtgaat
     1261 ttcgccaaaa ttgtaaacat tggcgatcgc gttgatgaat acattcaacg ttctggtctt
     1321 acgattaagc cctaatttaa cgataagccc ggcctccaaa tgcgggctgt tcctgcatgt
     1381 aaccagcata actaatcggc ttttttcctt actgtatata ttggcttttt atgtcgcggc
     1441 tttcttactt gctcggacac agaaggacca attatgtcta aataatctac aagctttgta
     1501 aggtcgtata tgttgcggcc accatatcgg ttgtcgcttt ttgatgtcgg ttttttatct
     1561 aagagattgg ctttttctag gtcattaata gccctttgca ccgtccttac tgacaccccc
     1621 atccgtatag ccaggctctc cattgagggg tgcggccact cctgtagatt ccaccagtgt
     1681 actatgagat tcagcaaggt attgaatgct acagggctca atttcttctc attttgtata
     1741 aaaaacaaca cagtaggtac tgcagtccat cctaatgaca gacctccagc cttccatttt
     1801 ttttctattg cgtaactcat tgattatcct gtaaatattt tttttgagat atatataata
     1861 taagtattca tatgaatagg gtgacaccag cgccattatt acacaactat aaaaggctaa
     1921 tctgagaaca gaaaaacatt tctatctgca ttaaatacgg agttagtgat atgaaaacca
     1981 aacaacatca tgatcttgta aaagatactg aaatatttgg tgtatccttg tctctgttga
     2041 tttccagagc gaatgagaga tctgttacaa tgccatctgg tctaagtagg gagcagagaa
     2101 gagcatgggc agcggatcaa gcacgcaaaa tccacaattg aatgttgtct ctttctctga
     2161 gacattcaat ctttattaca catcccgctt ttctcaaaaa acactcgata aaatcgatga
     2221 cttcatcgaa catttcgaga agtacaatct ttttggcgat gccttaaaag gccatccggc
     2281 ttggattggc aaggtatcgc catcttggaa cgtgcctgac agttacgaaa acaaagaagc
     2341 tattgaagaa catgccaggg ctaataaatt gtggcatgcc catataggcg atcccgtttt
     2401 catcgataca tttcacggga agtacaaagt ctctgattgg gtcatccact tccaacgtct
     2461 agcgccgaac catataaaac tgttagagct tgggtatcat gaccctatgc aactccccca
     2521 aaatttcgaa tgatctcttg ttcggatgaa tgatcccttg agattcgttg accctcgtcg
     2581 taatctcttg cggtgaaaac gaaaaaacca cccggcaagg tggctttttc gaaggttcgc
     2641 aagagttgac gctcttttga accgcggtaa ctggcttgga ggagcacagt caccaaaact
     2701 gtcctttcag tgtagcctta tcgcacccga aacttcaaga ctctcctcct ctaaacctgt
     2761 taccagtggc tgctgccagt ggcgctttgt cgtgtttttc cggattggac tcaagttgat
     2821 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     2881 tggagcgaac tgccttcccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     2941 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca
     3001 ccagggggaa acgcctggta tctttaagtc ctgtcgggtt tcgccaccac tgatttgagc
     3061 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgcgg
     3121 ccttatcgtt tccctgttaa gtttcttcct ggcatctacc aggaatttcc gccccattcg
     3181 taagcccaca ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3241 gcggaatata tcctgtatcg catattctgc tgacgcgccg gtgccgcctt ttttctcctg
     3301 ccacatgaag cacttcactg attcccgcac ccgtgccaat ataataagcc agtatacact
     3361 ccgctagcgc tacgtgactg gttcagggct gcgccccgaa acccgccaaa agcctgacgc
     3421 gcctgcggct tgtccaaccc cgcgccgacc gggaaaggct ttcccgcccg tcccggcgtt
     3481 atcaggaggc cggtttgtca gagaaacgga gcaagatgct caccatgtgg gtgacggagg
     3541 acgagcaccg gcggctgctt gagcgctgtg acggcaggca gctggcggcg tggatgcggc
     3601 agatctgcct ggacgaaaag ccggcacgtt ccggaaagct cccctcgatc tcgccggcgc
     3661 tgctgcgcca gcttgccggc atgggcaata acctgaacca gatagcccgc cgtgttaacg
     3721 ccggcggcgg caccggccat gacagggtac agattgtggc ggcgctgatg gccatcgatg
     3781 ccggccttga gcggctgcgg cacgccgttc ttgagaaagg aacggacgat gatcgttaaa
     3841 tttcatccgc gcggccgcgg cggtggcgcc gggccggtgg actatctgct gggtaaagac
     3901 cggcagcgtg aaggcgccac cgtcctgcag gggaagccgg aagaagtccg ggagctgatt
     3961 gacgcgtcgc cttacgttaa gaaatacacc tccggcgttc tgtcgtttgc ggaggccgat
     4021 ctgcctcccg gccagcggga aaaactgatg gccagcttcg agcgggttct gatgcccgga
     4081 ctcgataaag atcagtacag cattctgtgg gtggagcacg cggacaaggg gcgtctggag
     4141 ctgaactttc tgatccccaa taccgagctg ctgaccggca ggcggctcca gccttactac
     4201 gaccgcgccg accggccgcg catcgatgcc tggcagacta tcgtgaacgg cagactgggg
     4261 ctgcacgacc cgaacgcgcc ggaaaaccgc cgggcgctgg tcacgccgtc cgggctgccg
     4321 aaaacgaagc aggaggccgc agaagccatt acccggggac tgctgaccct tgcttcgtcc
     4381 ggggagctga aaagccgtca ggatgtcact gacactcttg aaggtgcagg ttttgaggtg
     4441 gtgcgcacca cgaaaagcag tatcagcatt gccgacccgg acggggggcg aaacatccga
     4501 ctgaagggag ccatctatga gcagtctttt aacgctggcg aaggacttag agcagcaatc
     4561 gaaagcgcag cagcagagta ccggcgagat gctgaaagcc gcattcagcg agcacgagca
     4621 gtctgtcaga gcggaactga gcgcaagcgc gaggagaatc agcgacgcca tcctcgccca
     4681 cgagcagagt atgagcgagg cgatggagaa aaaccggcgg agcgtactgc ggactgccgg
     4741 gcggacatgg ctcaccatcc tgatggtgtc agcgctgctg atcggcacga gcgggagcat
     4801 tctgtggtgg caggggcagc agataaccga caattacacg catcttcgtc agcaggaa
//
