LOCUS       CP056450                4197 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00503 plasmid pRHBSTW-00503_5,
            complete sequence.
ACCESSION   CP056450
VERSION     CP056450.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148639
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00503
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 131.583624931624x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 21:51:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,949
            CDSs (total)                      :: 4,821
            Genes (coding)                    :: 4,585
            CDSs (with protein)               :: 4,585
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 94 of 236
            Pseudo Genes (incomplete)         :: 141 of 236
            Pseudo Genes (internal stop)      :: 44 of 236
            Pseudo Genes (multiple problems)  :: 36 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00503"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00503_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3841..4197,1..783)
                     /locus_tag="HV222_24735"
     CDS             join(3841..4197,1..783)
                     /locus_tag="HV222_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="QLV52886.1"
                     /translation="MTDNIAATIKEKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVIDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="DNA (cytosine-5-)-methyltransferase"
     gene            1601..1795
                     /locus_tag="HV222_24740"
     CDS             1601..1795
                     /locus_tag="HV222_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLV52887.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQATMCERLHELA
                     EKLQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2814..3731)
                     /locus_tag="HV222_24745"
     CDS             complement(2814..3731)
                     /locus_tag="HV222_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="QLV52888.1"
                     /translation="MQRLSPGEFKTLISKERKSHFITPFALVYKTFCDLGYDQKNSDYF
                     LNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPYDAIRDFTMEYPTH
                     IYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFFQKNQIGKLVDLVM
                     PGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATLDDSFSEETINILY
                     EANVVVVTTIENKNSKYKNNNRVLTFEDMLQSAMELSRKWNNVSYTDSEKEEIQRSILK
                     QIEKYSDFPYVVNYYRNRLSALFD"
                     /name="restriction endonuclease"
     Typing          complement(1467..1565)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(1876..1961)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          1983..2533
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.213"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggggacataa caaaaatcga tgaaaaagat attcctgatc atgaaattct tgtcggtggg
       61 tttccttgtg tagcattctc tcaagcaggc ctcaaaaaag gatttaatga tacaagagga
      121 actttgtttt ttgatatagc aaggataata aaggaaaaaa aacctcatgc ttttttactt
      181 gaaaatgtaa aaaacttgct aggacatgat aaaggtagaa ctttttcaat aataaaaaac
      241 acacttgaag aattgaatta cactgtgtat tacaatatct tcgctgcaaa agactttggt
      301 gttccacaaa atagagagcg catatacatt gtaggcttta ataaagaaaa agtaagaaat
      361 catgaacatt ttacttttcc aaccccatta aaaacaaaaa caagagttgg agatattctt
      421 gaaaagagcg tagacaataa atatacgcta tcagatgcgc tctggaatgg acaccaacgc
      481 aggaaacttg taaatgcagc agccggtaaa ggctttgggt atggtttatt taatgaaaat
      541 agcccatata ctaataccat aagtgcgagg tactataaag atggtagcga aatattaata
      601 gagcaaaaag gaagtaaccc aagaaaaata actccgcggg aagcgtcaag acttcaaggg
      661 tttcctagtg attttattat ccctgtatcg gatacacagg cttataagca atttgggaat
      721 tcagtcgcag tccctgttat caatgcaata gcagaaaaaa tcatatcgac cttagattca
      781 taaactaagt catgaggaca caatgcggcc cagaatccaa ttacccccca tatggattta
      841 ccataacgtc gcattacgcg caccgctaaa acgcgctgaa cgcgattctg ccgcgaacac
      901 cgcaaaaaga gatgtttttt cccggggtaa accggaaaaa cgcgtcagga tcgctttcag
      961 ctcactgcat agctatgcat gaaagtgaat ggcgatcggt ttgggcgcat atggtgccca
     1021 tgagacacat tttcggacat tttcagttcg tgaatccctg actcattgct acatatctga
     1081 acagacaccc ctagcagcgc tcccaggggc gttatgtaaa aaaaggcgac gcgcccctag
     1141 gggcgcacct acattaatta actgtttttt ctacagatag tcagtactga ccctaagtag
     1201 ggtcgccata gcgtcgccat agggtcgtcg acgacgtgct tttgtataaa tctgaataag
     1261 aaaatatatt tttaaaacca taacaacatg atttaaaaag caaatcagca aaaagttggt
     1321 tttgcgtggg gtgtgggcat ttcgggtatg agaacagact cgcgtttttc cggagtatca
     1381 gcggaattat tgattaagaa acactcgatg cagagcgaaa ttttatgcaa cagcggctgc
     1441 actgcacagg gattatttat cagttgatat gctgtgccat gatacgtcct aaagaaacag
     1501 gacaggatgt gcaattgtaa taccgtcgcc cgcaacgtct tacaattacc acctgctcag
     1561 ggtccccccg acacccggaa ctgacggaga acaacggaag gtgaataaac aacaacaaac
     1621 tgcgctgaat atggcgcgat ttatcaaaag ccagagcctg acactacttg aaaaactgga
     1681 tgctctggat gccgacgaac aggcgaccat gtgcgaacga ctgcacgaac tcgcagaaaa
     1741 actccagaac agcatccaga ttcgctttga ggccgaaagt gaaacaggaa cataacgaag
     1801 ctgacggagg cggtcacggc tttcccgtga gcgttttcgg cagcagacaa gcccgtcagg
     1861 gcgcgtcagg gggttttagc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     1921 gcggagtgta tactggctta actatgcggc atcagtgcag attataagaa aagagcacca
     1981 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa tgccgcatca ggcgctcttc
     2041 cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggagtcggc
     2101 tcacgcaaag acggtaatgc ggttatccac agaatcaggg gataaggccg gaaagaacat
     2161 gtgaacaaaa aggcaaaacc aaggaaaaag gccgcgccgc tggcgttttt ccataggctc
     2221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2281 ggactataaa gatcccaggc gtttccccct ggtcgctccc tcgtgcgctc tcctgttccg
     2341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2401 catagctcac gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2521 tccaacccgg taagacacgc cttaacgcca ctggcagcag ccactggtaa ccggattagc
     2581 agagcgagat ttatccagat gcttacagag ttcttgaagt gatggcctaa ctacggctac
     2641 actggaagga cagtatttgg tatctgtgct ctgctgaagc cagattacct cggttagata
     2701 atctgccagg attagctaac cttcgaaaaa accacctgcc agggtggttt tttcgtttac
     2761 agatccaaga ttacgcgcag aaaaaaagga tctcaagaga tccttttagt tttttagtca
     2821 aaaagagcac taagcctatt tctataataa ttaacaacat aagggaaatc actatatttt
     2881 tctatttgtt ttaatattga ccgttgaatt tcctcttttt cagagtctgt gtaacttaca
     2941 ttattccact ttcttgataa ttccattgct gactgaagca tatcttcaaa ggttagcacc
     3001 ctattattat ttttatattt ggaattttta ttttctatcg tcgttacaac gaccacatta
     3061 gcctcgtaca atatatttat tgtctcctcg ctaaaagaat catctagtgt agctagatac
     3121 atttctctaa ttcctgttct attaacctct tcaggtactt cttgccatcg ctccctcagc
     3181 gttgttttag cggaaatcaa cattgtattt cttttgttag atgtgtactg gactacacct
     3241 ggcataacaa gatctacaag tttacctatt tggttttttt gaaaaaatga cttcccaata
     3301 gccccctgca catcaacagg aattcctgcc cccatcatta atagctcaag aatagactca
     3361 aactcttttc ctgccctgct acgtctggat tgagtgttag acagtgccaa atcatatatg
     3421 tgtgtagggt attccatcgt aaaatcacga atggcatcat aaggactcat atcttttatt
     3481 ctttcttcat caataagata tgataacatt cttgttgtga attctttttc aaatggttca
     3541 aattctttcc aacagttttt tctcattgca attatatatt cacttggatt attcaaaaaa
     3601 taatcactat ttttttgatc ataccctaaa tcacaaaatg ttttataaac caaagcaaat
     3661 ggcgtaataa aatgcgattt tctctcttta gaaattagag ttttaaattc accaggactt
     3721 aatctttgca taaaaaataa ccttttatat ctacttgaaa gttttttcat gcacgttcat
     3781 aattggaatc aaaacaggac aaattgtcct aaaaccaaca cttaattctg gtagacatgc
     3841 atgactgata atatcgcagc aacaatcaaa gaaaaaagag aaagacttca tatgactcaa
     3901 aaagagtttg cagacgccct tggtctttca aaatatggag acagaaccat aagaagatgg
     3961 gagcgcgggg aaacaaaacc tactggggcg gaactaaaag cagttataga ctttccagac
     4021 acaccacctt acccaaacaa cgagaatggc agatatcgca tgatcgattt atttgctgga
     4081 atcggtggaa ccagacttgg atttcatcaa actaacgctg taaatgttgt tttttccagc
     4141 gaatgggata agtttgctca aaagacatat catgcaaatt atggtgattt ccctgat
//
