LOCUS       CP056445                2495 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00508 plasmid pRHBSTW-00508_5,
            complete sequence.
ACCESSION   CP056445
VERSION     CP056445.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148640
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00508
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108.888855964456x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:54:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,666
            CDSs (total)                      :: 4,551
            Genes (coding)                    :: 4,447
            CDSs (with protein)               :: 4,447
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 34 of 104
            Pseudo Genes (incomplete)         :: 58 of 104
            Pseudo Genes (internal stop)      :: 27 of 104
            Pseudo Genes (multiple problems)  :: 14 of 104
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00508"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00508_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            363..554
                     /locus_tag="HV223_23320"
     CDS             363..554
                     /locus_tag="HV223_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV57335.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(845..1030)
                     /locus_tag="HV223_23325"
     CDS             complement(845..1030)
                     /locus_tag="HV223_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLV57333.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1396..1623)
                     /locus_tag="HV223_23330"
     CDS             complement(1396..1623)
                     /locus_tag="HV223_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QLV57334.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          711..798
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2039..2485)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.742"
                     /coverage="86.0"
                     /evalue="5.5e-153"
ORIGIN
        1 gtaagcccgt cccgctcgcc gcagccgaac gaccgagcgg agcgagtcag tgagcgagga
       61 agcggaatat attctgtatc acattttctc ctgacgcgtt ttctttcact ttctgcgcct
      121 gtcttatgtg gcattaatgc tatgtgttac tgccatgcta catcttaagc cagtatacac
      181 tccgctagtg ctccgtgact ggtccggcgc tgcgcccgga acccgcctgt accggttcag
      241 cagccgttcc ggcctgactg caattttttt tttttcatcc ctgcccgcta ccctgtaaac
      301 ctttcttctg cgttgccgtt aacagtaggt aagccatgtt tggtttttct tccggagccc
      361 tgatgactct tagtgatgat gagcgacacc tgcttgtgtc cgttgtttcg gtctggctac
      421 ggcgtgccgg aggggatgcc ggagccatga tgctggatgc ataccggcag attttgtctg
      481 aaaccgaacc ggccgtaagg actgtcatgc tcgaatttct tgaatccgtt cgcattcatt
      541 acatttcgtc ctgagcattt tctgtatttc tgtccatact taaacgaggc ggccgtcacg
      601 gacggtggtc gtcgttccat gatgctgtta tggacgagtg agagtaatct aatctaactt
      661 gattgagttc cgggcgtgcc gcccggtttt ttttgctgtt tcccggtgtc cgttccggga
      721 caggatgtgc atttgtaata ccgcgcacgc gccggcatca caaataccct ggtcagggct
      781 gcgccccgac accccgccat cgcctcacgg ccgcgcactg aattgcgcac ccgttcaccg
      841 attttcacgc attttcctcg cctatctgtg cgttcagcat ggcgtaaagc gcttcggcct
      901 gatcgtgcag gtgctcacag cagccggcag cctcatccag atccatctgc tcgagccgct
      961 caaggaggag aagtgtctgt gcgcggataa atttggccat attgagggcg gcggattcct
     1021 gtctggtcac tggtgatttt ccccgtctgg tgatgatttt tgcgtgaaaa gagcatggcg
     1081 tcattctggt gcatagtcat gcatgccgtt aaaatttaac aggcgcgttt ctgacgggtt
     1141 ccggggtggt ttgttgtggt ttttgtcatg gtttcgtcag gaacgcgctg aacgcgtctc
     1201 aggcggtgcg ggcaacggat gttatggtaa attcttgggg gcttgattat aaaataccgc
     1261 acttcctctt atgtcttgtg ctttttagac aaccctgaaa gcttttagct ttggtgatgc
     1321 aatttgctgg gattttggcg gagaaccaca ggtaaagaaa aaggccacat tagcggcctt
     1381 tttcggagaa gatgctcagt gcggaatttc ggagacttct ttaacgtcgg ttttattgat
     1441 ctgctgtttg acaccattcg cgtctgtata gccaagcagg cctgagtctg attctttagg
     1501 tttgccgtcg ctgacaatag tgcgaccgtc attggtgtgt atggcatagc ttgtgcgcgt
     1561 gcagccggtg agggctgaaa gcgcaactgc cgctgcgaac actgaaacaa aaatcttttt
     1621 caaagccagc tccttttatc catatcagtt ttggtcacta accatctaac tataggtcat
     1681 tttttatgtg aaaagaggct tgatggtggg ctgtatatcg agttaggcgc gaggtgctat
     1741 ggtcaaagtg tggtgtcagg cggggaggag gcttatatga gatgtaatcc ggccgataca
     1801 tataccaaga tgatttaagt attcctcttt gtgaggttgg atgtctaatt tataaaggat
     1861 cttcttgaga tccttttttt ccgcgcgtaa tctcttgccc tgtaaacgaa aaaaccaccc
     1921 tggcaggtgg tttttcgaag gttaggtaat cctggcagat cccctaaccg tggtaacagt
     1981 cttgtgcgag acatgtcacc aaatttgtcc tttcagtgta gcctcactaa ggccgccact
     2041 tcaagaactc ttgagacatc tctcgcacat cctgtttgcc aatggccgtt gccaatggcg
     2101 attagtcgtg tctttccggg ttggactcaa gttgatagtt accggataag gcgcagcggt
     2161 cggactgaac ggggggttcg tgcatacagt ccatcctgga gcgaactgcc ttcccggaac
     2221 tgagtgtcag gcgtggaatg agaaaccgcg gccataacag cggagtgaca ccggtaaacc
     2281 gaaaggcagg aatgcggggg agcacgaggg agccaccagg gggaaacgcc tggtatcttt
     2341 aagccgcatc gggtttcgcc accactgatt tgagcgtcag attctgtgat gcttgtcagg
     2401 ggggcggagc ctatggaaaa acggctttgg cgcggcctta tgctttcttc gttaagtatc
     2461 ttcctggcat cccccaggaa atttctgatc catcc
//
