LOCUS       CP056444                4783 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00508 plasmid pRHBSTW-00508_4,
            complete sequence.
ACCESSION   CP056444
VERSION     CP056444.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148640
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00508
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108.888855964456x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:54:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,666
            CDSs (total)                      :: 4,551
            Genes (coding)                    :: 4,447
            CDSs (with protein)               :: 4,447
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 34 of 104
            Pseudo Genes (incomplete)         :: 58 of 104
            Pseudo Genes (internal stop)      :: 27 of 104
            Pseudo Genes (multiple problems)  :: 14 of 104
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00508"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00508_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(372..614)
                     /locus_tag="HV223_23285"
     CDS             complement(372..614)
                     /locus_tag="HV223_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="QLV57327.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="protein L"
     gene            801..1118
                     /locus_tag="HV223_23290"
     CDS             801..1118
                     /locus_tag="HV223_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QLV57328.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1321..1677
                     /locus_tag="HV223_23295"
     CDS             1321..1677
                     /locus_tag="HV223_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="QLV57329.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antidote protein"
     gene            2770..3093
                     /locus_tag="HV223_23300"
     CDS             2770..3093
                     /locus_tag="HV223_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLV57332.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3032..3796
                     /locus_tag="HV223_23305"
                     /pseudo=""
     CDS             3032..3796
                     /locus_tag="HV223_23305"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3771..4262
                     /locus_tag="HV223_23310"
     CDS             3771..4262
                     /locus_tag="HV223_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLV57330.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            4269..4484
                     /gene="mbeD"
                     /locus_tag="HV223_23315"
     CDS             4269..4484
                     /gene="mbeD"
                     /locus_tag="HV223_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLV57331.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="mbeD"
     Typing          2482..2655
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(2007..2515)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2771..3087
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
     Typing          3084..4576
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcggatttta gtgtgacttt aacatccatg catctggctg catgactatg caccagtgta
       61 atattcagcg gctgcgtttc tggtgttttt tcgttatgtc tgtcgccact ttctcgcgtc
      121 tggcaccgga aacgcagcca accccgtttc agcggtgcgc ccaacggatg ttatggtaaa
      181 tcagtatcag ctgcagatat aaaaaagccg ccaggctggc ggcttgctgt tattttcgtg
      241 gctttcctgc atctgagcgt tttcctctga cccttccgtc attgttacgg tcacgaggtt
      301 gagttccgcc gcctggttta ggaacgacaa cgtgtgggtt gagtggtcga cgatctgcca
      361 ttttctatac ctcactgagc ataaacaaac aattgccaac gaatgttccc ttgcgcgggg
      421 gtatgttggt ggtggttttg tggtgggatt gttttcccgc ctgcgatagt tacttcttta
      481 ccgcaaccgg tacatttata aatcccttca tattcacatg tttctcccgg cttatattca
      541 gtgtcaaaaa ccgagttaaa cttcgtatct gcttcaaaaa tcttgctttt gtttcctgag
      601 cgaacaagag ccatgcaacc tccaaattat catttatttt gcacacccgc actgtgcatg
      661 agtgcattct acagcatagc cattgattgc acaagtgcac tgtgctgttg tgcatttgat
      721 atgtttgctg tactatatcg gaggtaagaa ggtgatcgaa aaaattgcca agttgcggca
      781 aaaattgagg taaaagaaaa atgcagcaag atcatgaaca tgatgattgc cgcattcagt
      841 ttcgagacga ttggctatac cagttttaca aatatggtaa agccaatcga aagattccaa
      901 ctgcgattag tgcttcatta ggtcgcaagc tggatatgct gaatgctgct gataaagttg
      961 gagatttgcg gtctcccccg ggaaaccgat ttgagaatct caaccctcca ttgacagggt
     1021 attcatccat acgggtgaat gagaagtacc gcttgatatt taagtggggt gaaaacggag
     1081 tcgaggattt gtatctcgat ccacatgatt accgatagat aacaggtcgt ggctgttatc
     1141 ttataaaaca agtaggtgag gcgttccctc acctacatta aaatgtcatt acaaataatg
     1201 attaataaaa aaaatgacgt gactatattt aagataatta ctcatcgcta cagctggtaa
     1261 tagacgaaat acttatcttc ctcatctaac agagagaagt aaaaaattta ggagtaaagc
     1321 atgtcccaag caactagaaa accatcaact gtaggggata ttattcttta tgaatatctc
     1381 gaaccaagtg gtttaaaaat caatgacttg gctgaaatgt tgaatgtgca tagaaactct
     1441 gccagtgctt tagttaacaa taatagaaag ctgtctaccg atatggcttt taggttggct
     1501 aaagccttcg ataccactgc aaagttctgg ctaaatatac agcagtctgt tgatttatgg
     1561 gaggttacta gtagccctcg attgcaagaa gaaatagaca gagtgattcc cgctcatgaa
     1621 atcatagaaa ggaaacacat tgttgatagt tctgagaaga aacatgctat tgcatgattt
     1681 tttttaaaaa aatataagtt atccctcaaa aagaaggctt ctataactag aagccttctt
     1741 tttgagggat aaaaacgctc atgttaactt tcttcttacg atcttcttga gatccgtgct
     1801 ctgtacgcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc ggtttttcga
     1861 aggttaagtc agttggggaa ctgcttaacc tggtaactgg cttcagcaga gcgcagatac
     1921 caaatactgt ccttccagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgttttgt
     2041 cgtgtctttc cgggttggac tcaagtcgat agttaccgga acaggcgcag cggtcggact
     2101 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaaccgagtg
     2161 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     2221 gcaggaacag gagagcgcac gagggagcca tcagggggaa acgcctggta tctttatagt
     2281 cctgtcgggt ttcgccacca ctgatttgag cgtcagattc tgtgatgctc gtcagggggg
     2341 cggagcctat ggaaaaccgg ctctgccgtg gccttatcgc ttccctgtta agtttcttcc
     2401 tggcatcttc caggaaattt ccgccccgtt cgtaagcccg caccgctcgc cgcagccgaa
     2461 cgaccgagcg cagcgagtca gtgagcgagg aagcggaata tatcctgtat cgcatattct
     2521 gctgacgcgc cgttgccgcc ttttttctcc tgccacatga agcacttcac tgatttccgt
     2581 atccgtgcca acatattaag ccagtataca ctccgctagc gctacgtgac tggttcaggg
     2641 ctgcgccccg aaacccgcca aaagccactg acgcgcctgc ggcttgtcca accccgcgcc
     2701 gaccgggaaa ggctttcccg cccgtcccgg cgttatcagg aggccggttt gtcagagaaa
     2761 cggaacaaga tgctcaccat gtgggtgacg gagggcgagc accggcggct gcttgagcgc
     2821 tgcgacggca ggccgctggc ggcgtggatg cggcagacct gcctggacga aaagcctgcg
     2881 cgcaccggaa agctcccctc actctcgccg gcgctgctgc gccagcttgc cggtatgggc
     2941 aataacctga accagatagc ccgccgtgtt aacgccggcg gcggcaccgg ccatgacagg
     3001 gtacagattg tggcggcgct gatggccatc gatgccgggc ttgagcggct gcggcacgcc
     3061 gttcttgaga aaggaacgga cgatgatcgt taaatttcat ccacgcggtc gcggcggtgg
     3121 cgccgggccg gtggactatc tgctgggtaa agaccggcag cgtgaaggcg ccagcgtcct
     3181 gcaggggaag ccggaagaag tccgggagct gattgacgcg tcgccttacg tgaagaaata
     3241 cacctccggc gtactgtcgt ttgcggaggc cgatctgcct cccggccagc gggaaaaact
     3301 gatggccagc ttcgagcggg ttctgatgcc cggactcgat aaagatcagt acagcattct
     3361 gtgggtggag cacgcggaca aggggcgtct ggagctgaac tttctgatcc ccaataccga
     3421 actgctgacc ggcaggcggc tccagccgta ctacgaccgc gccgaccggc cgcgcatcga
     3481 tgcctggcag accatcgtga acggcagact ggggctgcat gacccgaacg cgccggaaaa
     3541 ccgccgggcg ctggtcacgc cgtccggact gccgaaaacg aagcaggagg ccactgaagc
     3601 catcacgcgc ggactgctga cccttgcttc gtccggggag ctgaaaaacc gtcaggacgt
     3661 cactgaggcg ctggaacggt caggttttga ggtggtgcgc accacgaaaa gcagtatcag
     3721 cattgccgac ccggacgggg ggcgaaacat ccgactgaag ggagccatct atgagcagtc
     3781 ttttaacgct ggcgaaggac ttagagcagc aatcgaaagc gcagcagcag agtaccggcg
     3841 agatgctgaa agccgcattc agcgagcacg agcagtctgt cagagcggaa ctgagcgcaa
     3901 gcgcgaggag aatcagcgac gccatcctcg cacacgagca gagtatgagc gaggcgatgg
     3961 agaaaaaccg gcggagcgta ctgcggactg ccgggcggac atggttcacc atcctgatgg
     4021 tgtcagcgct gctgatcggc acgagcggga gcatactgtg gtggcagggg cagcagataa
     4081 ccgacaatta cacgcatctt cgtcagcagg aagataccct ggcgaaaatg actgcccgga
     4141 cgtggggagt gcgctatcag gagagcagcg acggccggag gtttcttatc ctgccgcccg
     4201 ggatgcaaac cgaagccatc ccctacgacg ggacgacatg gatccgcctg aaacaggagt
     4261 agcggaaaat gacagagctg gaaaagcagt tgctgagcgc attagagcag ctacagcagg
     4321 actactcgca aaggctggac gaatgggaga gcgccttcgc ggaatggcgg agcatgtgtg
     4381 ggcttatgca acgggagaac gcggtgctga acgagcgcgt gtcggacttg agcacgcagg
     4441 tgctgagttt aagcgagcag ctgcgccgct tgtccgggaa ctgaatgcca tcgagctccg
     4501 gcagagggag aatgagtatc agaaaacact ggagctggag cgccagccgc agaaaaccta
     4561 ccgcgggccg acactttaaa ccatccaggc agctgacaac ccgcacgaag aggggctctt
     4621 atttctcccg ctaaataggt tcgtgatcaa aaaggtcacg acttattcag gagcgggaaa
     4681 tgaagctgtt aaatctcatt gaatcattgc ttaactgcct gtatctggcg gcggagctgt
     4741 gggactggct ggagaagcat ttgtcggttt ttaaggcatt ggc
//
