LOCUS       CP056406                7461 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00562 plasmid pRHBSTW-00562_3,
            complete sequence.
ACCESSION   CP056406
VERSION     CP056406.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148652
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00562
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.1724665296571x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 20:45:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,500
            CDSs (total)                      :: 4,379
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 72 of 157
            Pseudo Genes (incomplete)         :: 75 of 157
            Pseudo Genes (internal stop)      :: 33 of 157
            Pseudo Genes (multiple problems)  :: 22 of 157
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00562"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00562_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(258..1916)
                     /locus_tag="HV235_22445"
     CDS             complement(258..1916)
                     /locus_tag="HV235_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="QLV66380.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            2077..2424
                     /locus_tag="HV235_22450"
     CDS             2077..2424
                     /locus_tag="HV235_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QLV66381.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEAN
                     VTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLYCP
                     QADPDKVKKQP"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2968..3189
                     /locus_tag="HV235_22455"
     CDS             2968..3189
                     /locus_tag="HV235_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV66382.1"
                     /translation="MTKLDENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     gene            complement(3243..3776)
                     /locus_tag="HV235_22460"
     CDS             complement(3243..3776)
                     /locus_tag="HV235_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV66383.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3779..4705)
                     /locus_tag="HV235_22465"
     CDS             complement(3779..4705)
                     /locus_tag="HV235_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLV66384.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHCFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4882..5280
                     /locus_tag="HV235_22470"
     CDS             4882..5280
                     /locus_tag="HV235_22470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV66385.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(5474..6409)
                     /locus_tag="HV235_22475"
     CDS             complement(5474..6409)
                     /locus_tag="HV235_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLV66386.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            6782..7234
                     /locus_tag="HV235_22480"
     CDS             6782..7234
                     /locus_tag="HV235_22480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV66387.1"
                     /translation="MEGSARRFSVSELHVSPGQKKTDQGSGQESESVRNFFSCGMRQCS
                     QPPQNTLRLIKNNWHYPVPDKKTTKVGNPRETIGVRLSQNIDNEIVTQINLAASLILIQ
                     YSIKNARIINHIYKDIYQTKQPSSQLRIRIFLYVIDKTQFFINCNI"
                     /name="hypothetical protein"
     Typing          complement(5475..6409)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.902"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3783..4705)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.078"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaaaacaa aacacaataa aaacaatgaa ttatataaaa ctcaacgata tctttgtacg
       61 gagatttaca ttcaggaaca ttggggtcag tttggatata gagaattatt gtacggtaag
      121 cctgtttttt gaacagtcca cattatcgca atagggtacc atccagggtg ttaattattt
      181 ttgtagattt ctattccata taatacgttt tcaatgaatc tggaagatga ggtgccatgt
      241 gtatggcacc tcatctctta ttgactcatt gattccgtaa ccgcctgcca agttataaag
      301 ccaccactca gattatatgt ccggaagccg tgagactgaa gcatacggta tgccacatgc
      361 ccccgcagac cactctgaca cccgataata atctctttat cttcaggcaa cttatcaaga
      421 ttctcccgca gggaatcaag agggatggat agcgcgtcag gatattcacc aaattgtttc
      481 agttctgcag gagaacgaat atcaagtaaa caataactgc ccggttcccg ttgtaacaca
      541 tcgcggacat gacagatgac cgtatcgccc ttgataacat ttgccgccac cattccagcc
      601 tggttaacga cgtcccttgc actgttgaaa ggcggagcat aggtcagctc cagatgctcg
      661 agatcgttaa cggtaagccc cgcgcgctgt acgacagcca ggacatcgat gcgtttatca
      721 accccttttt tccctgacgc ctgggccccg agaattttac cggtatcggg gctgaacagc
      781 agtttcagac tgatcattgt tgcccccggg tagtaactgg catgatcagc ggcatggacg
      841 taaaccttct cgtatcgggt accatgggct ttcagctgct tttcattcgc cccgacactg
      901 ccaatactca gcgaaaatac cttacaaatg gatgtcccct gactgccatg gtaaaggctg
      961 tgacgatcga gcatattatc agcagcaatg cgtccctggc ggttggctgg ccctgccaga
     1021 ggaacattag cgggttcctg aaagacaagg tcaggtgttt caactgcatc ccccacggca
     1081 tagatgtcag ggatacttgt ctgcatacag gcattgacac ttatgcctcc ccgtttaccc
     1141 acggcgagcc cggctcctgt ggcaaggctg ctctcaggtt ttaccccgat ggcgagaata
     1201 accatgtcgg tctgcagaaa cccaccgtca gacagtgcaa cgcggaatcc cgtctctgtc
     1261 cgcagtacct cggtgagcgc cgttctgaga cgcagatcca cgccatgact cctgatttcc
     1321 tgatgcaggg ccgatgccat ttcaggatca accggagcca taacctgttc ccccatctcc
     1381 agcagggtca cactgatccc cctttcgctc agtgcctcca tcacttcgag cccgataaat
     1441 ccccccccga caagcgtggt gtgagcgata ttatgttgct caatccacgc ctggatagca
     1501 tccatgtcag taagatttcg cagtgtaaag acacccgctt cctgtagtcc gggcaagggg
     1561 ggaacaaccg gagcggcacc ggtactgagg agcagacgat cccattcttc ggtatagacc
     1621 tccccggagg tcagattttt aaccgtcacc atcttattaa ccgggtctac tgacatcact
     1681 tcatgacgga tacggacgtc tatgttaaaa cgggatttaa aatcctccgg ggttttcaga
     1741 atcagcgact gccgtaacgg aatttttccg cctatatgat aaggcaatcc acagttggcg
     1801 aaagagacat cgcttccccg ctcaaacaca acaatactga catcctctga taaccgacga
     1861 gccctggctg cagcggatgc tcccccggca acaccaccaa caatcactat tttcattaca
     1921 tcctctctgt tcactcgggg caaaatatct gccatatata attagttacc cggtccagct
     1981 ggacgctgca gacctgaaga tcacggtggc aaaattacgc cactatcatt atactgaaca
     2041 atattatata aaaatacata atataagaaa ccagcaatgc ccatgaatga agacacaaaa
     2101 cgtcagactg atatgaaaaa tgcagccctg ggggcagcag atgtcctacg gggactggcc
     2161 aacagcgacc ggctattgtt gatgtgtcag ttaagccagg gggaagcgaa tgtgaccgag
     2221 ctggaagcgg cggtcggtat cactcagccg accctgtctc agcagttggg tgtcatgcgt
     2281 cgcctgaagc tggtcgagac ccgacgggca ggtaaaatgg tatattaccg tatcggggat
     2341 aaccgcatca tgacgttatt gaatacctta tatgatctct actgcccgca ggcggacccc
     2401 gacaaggtca aaaaacaacc ttaacgtaca ataattttcg atatccaaac tgcccccaac
     2461 attcaccagc acagttagac attaacaatg caaaatcaat gcgattccgg tgccgtatgt
     2521 cagggagaat tatgtaaacg ggggggggcc ggaatcgcat tccagctcat atcattaaat
     2581 acagaaaaaa acctctgaga acacagaggg gataaggaat atgaatactc cggtcaggat
     2641 attatcctag cttttatata caacatgctc acaaggacaa caactagcta gttaatagat
     2701 attaaaaatt ttagagctga aaccttaaag cttgcttcca ttccatgttt tattataaac
     2761 cttatcttga aaactgaaag gaagtacggc agattaatta agttgcgtgc attcttttca
     2821 cgttataata caaacaagaa cacctgcgaa gttatttctc cttacatatc cccctatacc
     2881 aacgggggat tttttctggt tcactaacgc ataaataata gtgacatact ataattcacg
     2941 aaccgtgata atatcagatc aatcatcatg acaaaattag atgaaaatat cccacatatt
     3001 atcgatgcag ctgtagattt tatggcatca agccaggcat ttcgtgagta tctgaaaaaa
     3061 ctcccccctg ataacacaat accgccagat cttactgatg agcacgtacc gttatatata
     3121 cgaaggctgg aatactatcg actgctttat cgaccaaagc agacagaatc atattggaaa
     3181 aaacggtgaa cggttcgcga aatcctttcg cgctccgtga ggcgttggac aagcgaagcg
     3241 cgtcagtttt tccggctctt ccccgtgaca ggtttctcca ccagtttatc ttccagtgtc
     3301 acaactttct gatacaccgc cgctgtggcg ttgtagttcc tgtcgagtat tgttttcatt
     3361 ccccatccga gcacacagaa cataaccaga aaagccactg acaccccacc cagccatttc
     3421 cagctatacc ctttaagggt gttgtattcc tgcacgatat tgctgtgaat tgtcgcaata
     3481 tccctgttca gggcatccgc gtttgccttc atgtttgcga ctgactcttc cagccgctga
     3541 aaatcctggc tgatgcgctc acctatcagt cctgccacgt ccagcttctg cacctcgttt
     3601 ctgacttcat ccctgacctg ttttgccaca ccgtcagaaa tatcccgata tccgcgaaga
     3661 gtttcccttg ccacagccag agaatcagca acagctttct gttgctctat tgcggtatgt
     3721 tgcagatgct ccaccgataa caggagcgtg agtaattttt cttctgtgct ggccatgttc
     3781 agaatcccat agaaaatcca cgtgacggtg atttaacccg ttcaggttca gttcggttct
     3841 ctctgctcac tccgtctctc atgtcaaagc aatgctcccg cgcaacatca tgttgcagcc
     3901 ttctgaccag ttcatgttgc ttaaccggtg tatccagcca ttccctgatg tcatgcccga
     3961 ccattcgcgc ctgttcaaga tggcgggaat agtccggttc agaccgtttt tgcatgatgt
     4021 catgacgctg ggcatcatca agtcgtccgg cagcttcaaa acccagatgc acttcgggac
     4081 gtcgttcaat gccctgttct ttcagtgtcc ggcagtcaat gaagcccctt tcaaagccat
     4141 gctttctcat atgttcgttg tgcttcacct cccagcgttt tcgcaattcc cgcaactgct
     4201 ctttttgctg agtgggagtc aggcttccgg aatccttttt cgcacctccc cgctcgggat
     4261 acctggcgtt atagcgccgg aaatactgtt ccggtgtacg gtcaatgcca tcattcacac
     4321 gctgactcat catgatatgc gcatgaggct gttcgccgcc gtcaatgctc gctttcggat
     4381 tgtgaatggc aaaactccag gcgtgccgtt cgccggcttc ctgacgtaca aaatcctgca
     4441 ccagttcaag gcgctgttcc ggtgtcagtt cacggggtaa tgcaatttca agttccctat
     4501 aggtcgaacc gttcgcacgt tcaaactggt cagccgcctg ccagaaatgg gaaggattat
     4561 ccctggccca ttccggcatg tttccgtgtg cggaatattc caaatcctga cggtcgcggt
     4621 atttctcttt gcgctcgatg taatcggcgt ggtttgcagc ctgccccttg ccaccaaatt
     4681 taacgctgag gtgataggtt gccacggttt cagccctccg caggagcgca ctgtttctcg
     4741 acgaaggaga gaaacatata agtgcgcttc cttctaatta ttcgaattag cacggaagat
     4801 accccctgca cccgccgacc gcaagcgata tactgcacga ccacaaaccg ccgtaaaaaa
     4861 acaccaggga gtaccacaaa catgcgcatc agcaacgtcg aatggctgaa gaaacggatc
     4921 ggattcatca ggaagctcgg agaacagacc gcgcgacaga gacagattat tgacctgctc
     4981 gacaacgaag ccggactcac agaacaggaa cgcaaacttc tccacgtgct ggcaacagcg
     5041 gaaaaaaacg acttacaggc acaggagaac gagcgaaaac agacgatcca gaaacgaatc
     5101 gagggaaaaa aacaacgacg ggaacgcaat caccgactct ttctcgcggc tgggctactg
     5161 atcgaagcag gactggtaga cacaaaaaca ggcgagctat gctataaaaa agacaggata
     5221 ttacaggaac ttaaggaaat taagtacgat ctggagacat ccccaaaccc agacgcgtaa
     5281 gtgcctccat ccgtgttccg gttccctggt gcttcctcgc tcattcgctc gctacgctcg
     5341 gtcatgagac tgcggcgagc actaccagca caatcatgcg gaaaacagca ccattgcagg
     5401 gctgaacggg gaaaaacggc aaaagacctg ctgctcaaaa gaacgccaga aaggcgctgt
     5461 tatctgatat aggctatttt ccgcttttcc agtttcgaat ggttcgagta gaaacctcca
     5521 acatcgccgc aatagctttc tgagaatatc cattctccag catacatagc gccatgaaac
     5581 gcttgtcatc gtaggcttcg cctttagcga tgccaccttt cctgcctctc ttggcctgta
     5641 tttctggcgt gtgcgtatca gccacatact gtgcgaaagt ttccggcgtg aagttcctgt
     5701 gcgtgtattt cgcaatactc ttgccaatag cccgacattc aggaggtgaa agcggaacgg
     5761 gaagcgatgc gttgtacatt tcgacacgct gaatcacggc atcaagccac tgtgagaatg
     5821 ctggccagcc ctgacgaatc gccctgtaag cccatttgcg cgtcatttcg aacaggtggc
     5881 agtttcgccc catcccgtaa tgtttatcga tgctacgacg cgctgaggcg ctcaaatcga
     5941 gataatcagc cagttcatcg agggtatagg cttcctcgcg ccactccatc accagccatt
     6001 ccaggtggaa cggatttttg caaatcaggc cgctgtagtt cacatccgca caaagtttct
     6061 cacacagcga acgttcaacc gctgcggcat atttcagcgc cttaaccgag gcatcaggcg
     6121 cggttctcac ggcgatgttg agtgcgtaga gcaggtgagc gtgtccgttc acaggatttt
     6181 taacggtgat gttcggcgcg ggagcgttcc ggtcgctcca gtcaatcgca gccccctcgc
     6241 ggtcaacgtc aaaaaccagc cagaactgtg catgaggctg gttctgctgg atataccgtg
     6301 caagaagcgc gcgccctttg ccggagatac gaaggccgaa agcgagatca tccgtgtgat
     6361 agggacggtg gggtaaattt tcttcgaagt attgaagcgc ggcgctcatg tgttacgatt
     6421 ccttccaggt tatcgaccga catcgcccct gtattttcgt gaaagtcttg gcggatagac
     6481 acgaatttca ggggcgtttg tttttcagtc gcttacacta cacccaacgg caaatcctgt
     6541 cgatccttcc cgaaaaaccg atccatttac cataaatccc gttgtacgcc ccccttaaaa
     6601 cgccctcagt cgctttctgg cgcaaaaaac gcaaaaagac aagaaaacac caggcaaaac
     6661 cagaaaaacg cgtcagaatc gttttcaggg cgttgcataa ctatgcacga aagcgatgtg
     6721 cgatcggttt cgtgcgttct gaacaccgaa cggctcaaaa cgaaaagttt tctccccccg
     6781 gatggaaggt tctgcccgga gattttctgt ttcagagctt cacgtttcgc caggacaaaa
     6841 aaaaaccgac caagggtcgg ggcaagaatc agagtctgtg agaaattttt ttagttgtgg
     6901 aatgcgtcaa tgttcgcaac cgccccaaaa taccctgagg ctgataaaaa acaactggca
     6961 ctatcccgtt ccggataaaa aaaccacaaa agtggggaat ccaagagaaa cgataggtgt
     7021 cagattgagt cagaatattg acaatgaaat agttacgcaa ataaatttag ctgcatcact
     7081 tattctgatt caatactcaa taaaaaatgc ccgcataata aaccacattt acaaagatat
     7141 atatcaaacg aaacaaccaa gttcacagct caggataaga atttttctct atgtaataga
     7201 taaaacacag ttttttataa actgtaatat atgagctgta atattcatgt attaattgtt
     7261 tttttgaaaa gcatcacaaa tttatttttt acgcatactg ccctattgca accagcaatg
     7321 gttttcactt attttcaaca tctcatttaa tacgagttat acttcaaccc gtttaaagtg
     7381 caacactaac tgagtatcat ttgttttcct tacctacccc tggattccca ggggtttttt
     7441 ataactacaa accagcacaa c
//
