LOCUS       CP056360                4073 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00670 plasmid pRHBSTW-00670_4,
            complete sequence.
ACCESSION   CP056360
VERSION     CP056360.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148677
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00670
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.2574946361378x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 20:06:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,965
            CDSs (total)                      :: 4,839
            Genes (coding)                    :: 4,683
            CDSs (with protein)               :: 4,683
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 72 of 156
            Pseudo Genes (incomplete)         :: 97 of 156
            Pseudo Genes (internal stop)      :: 37 of 156
            Pseudo Genes (multiple problems)  :: 40 of 156
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00670"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00670_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            42..296
                     /locus_tag="HV260_24815"
     CDS             42..296
                     /locus_tag="HV260_24815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLW67568.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobilization protein"
     gene            830..1531
                     /locus_tag="HV260_24820"
     CDS             830..1531
                     /locus_tag="HV260_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLW67569.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1789..3936)
                     /locus_tag="HV260_24825"
     CDS             complement(1789..3936)
                     /locus_tag="HV260_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLW67570.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     Typing          complement(3579..3937)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3938..4073
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.48e-65"
     Typing          831..1531
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1793..4023)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcgctaaaa tgcgattttt catcgcgcac ggagttacat catgagcaga acgctcgaac
       61 agaagattgc tgatgctgag gcgagattac agcgcctgaa ggcgaagagc cggagcctgg
      121 acacagcgca gaaggttgtt gtgggtgctg cattgctggc aaaggtcaga aagccggagg
      181 aggtgcagtt gcgtgcctgg ttactccagt tcctgaaggc agaggtgaca cgacaggctg
      241 atgtgacgcg catactgccg ctgattaacg agctggaggc gctgccagaa cagtgattgt
      301 atcgtccgct cgccgcagcc ccagcgaccg agcgttagcg agcgagtggg cgaggaagcg
      361 gaagtgaatc agtcgccagg ttgagcacat acgcacggtt tttcggcagg tgatatgcgc
      421 tgaacccgca tgaatactgg gtttccacag ggttggaggg ggtgtattcg aaacttgcaa
      481 ttctgcttta ttgcaacgca ttgtttttat tggttttttg ttttcagcac cccaagttat
      541 ccacaggctc gccgttaagt ttttaagatc ttatttttag atcttttttt ttaaaacctt
      601 tttaggtctt gtaacaccat gattttaaag atgttctggc aagataagtg gagcttttgg
      661 gggtgaaagg tggagctttt gggggtgaaa ggtggagctt ttgggggtga aaggtggagc
      721 ttttgggggt gtaatccaca gaaaaagggg gttgcaaaaa aaaaacttcc acccatatga
      781 taagtggcgt acagtaaaaa aacgccacca atagcaacga gcaagcgaaa tgtcagatct
      841 gatagcttac aaatcaaacg cccttatcga ggcaagctac aagctcactt tgcaggaaca
      901 gcgcttcctg ctggtctgta tcagccgcct aaaatccgga gaagattccc cttcgccgga
      961 tgaacaaaaa accatgacca tcacggctgc agagtttcat gatgcctttc ctgacatggg
     1021 acggcagcac gctgaagcta ggctcaagga agcgatagat cgcctctggg acaggtcagt
     1081 aatcatcaag aacgaggaaa aacgagaaga gttccgctgg gtgcagtacc gggcgcagta
     1141 tttcaaaggt gaggcgaaag tagatatcac cttctccgat gccatcatgc cgtatctgac
     1201 acagctcaag gggcagttta cgcgggtagt cgtgaagaac gtctccagcc tttccagcac
     1261 ctacagcatc cgcatttacg agctgctcca gcagttccgc agcaccggtg acagaacgat
     1321 agctatcgat gatttccgaa ccatgcttgc cgttgaagat aaatatcaac aattcaaaga
     1381 cttgaataag atgataatca agccagccat cgccgaactg aacgaaaaaa gcgatctggt
     1441 tgtaactgtt gagacgatca aaaaggggcg cacggttgta gccctgcatt ttcgcttcaa
     1501 ggaagacaaa cagatcaaga tgacgatcta aaatcgattt taaggcacct aaaacgctcc
     1561 ctgctgcgtt ttttttgtgt tagtagtgcg aaggtactat tgaagtgaaa aattgcgtca
     1621 aaaacgctct gatgcagcaa aaaaagaccc cccaattttt gggggcggaa aaacaaaact
     1681 atgcttgcgt tatgtgagtg tgcttcttgc attcatccct gtcgatgaac atcgaagaac
     1741 atcgacgaac ataacacgat gttaaacaaa acaaaatatt gttcacattc actgtgacca
     1801 accgcgatca tgtctcctct cggtggtgtg tgttcgtgca tcttcctcgc gctgccattc
     1861 ctcccaggta cgattaaaac ccagcgagcg gaacagatga tccggctgtt tttctggtgt
     1921 gttgacggca gcaaaaagcg gtgcctcacg ggttttcaga tgaaactgca tcagttctgg
     1981 tgcaggtgat atgtggctgt ctgcatcgtg atttacggca acaccatacc ctgcataccg
     2041 taacccctta tcggtcagcg cactgatgac cagatgcctg ttatcggctg agggaacggc
     2101 aaacgcggga tacagcgtac cattaagccg gataacaccg cccagagagt catccacgcc
     2161 catgaccagc aaaagctgaa gctggcgggt ttcccggtcg acagaactca tgaccggcat
     2221 cagcgtcggt ggcagacgtc cctgcataaa atccaccttc cttgccgctg gtgatggcat
     2281 ggctggctgg cggtcttctc caagacagaa agactccggc catgtcttcg gctggcggag
     2341 attgcgggta tgttgcggcc atgtctggca aacctgcgtc agcagttgct caaaggttgc
     2401 agaggaataa acacgttgca gtggcggacg ttggccgatt tcgcttcggt cacagatata
     2461 accactcctg tcacgcatta aatcaacact ctcaaagatt ctggtgcggt atttctccag
     2521 ctcgttcccc cagagcgtat attctccgtt ccggttgtga agcagaaggt aacaggtcat
     2581 ttccccgtta ccaggcatgg gagcctcgcc ataccccgta acgactgctc tcagcacccc
     2641 tgaatccata acggcattgt catttttcct gagctgctcc gcgcgactac gttcagtatc
     2701 ttcctgttcc ttccggcgca attcctcttc acgtttaagc aactgggctc tgagagctgc
     2761 aaggccggat tccccccact gctgccactg tttttcaaat gccatctgac tcgcgggaat
     2821 gtcctgtttc tccaccttcc ggtaatgctg tcgtaatttt tccatttgct ggctcagaga
     2881 ctggattttt ccctgctcac tacggtattc cggtgccgcc cattgcatca ggggaatacg
     2941 ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg gcagcatggc tcatcgcttc
     3001 ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat
     3061 cgacatggca ttactggtga gtttccatgc cgagttactg tccttcggca acgcttcgat
     3121 aatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc ccgatattca gaaggtcagc
     3181 cacacggcgg gcaggctctg tcagccctcc gttcctgagt tcggtgcggg aaatctgtat
     3241 ttccaggtta agcctgttaa tcagacgatt ctgttcgccg cgttctgttt cgataccgcg
     3301 tgtttccatc gcacaggcag actttccgag gtgaatacca ggcgttttct caagcccctg
     3361 tcgctcgtat gaacgatggt ctatctcttc ctgataaccg gcgttcgcca gtgccctgtt
     3421 tgcatggtca gcccatgatg cccgccacgt ctcggcatgt gtccggtcgt tccagtccct
     3481 gacctttccg ctgaacccag caggtccgat agccctcgtg gtcagcatga tgtgcgcatg
     3541 gggatttgtt ttatccatgt gatggaacgc cacatcagca atcatgccct gactgacaaa
     3601 gttttctctg acaaaggcga gaacggtttc ccgtgcggca tcctgcggca gttcgtgggg
     3661 gatggcgagt tcaatttccc gcgcaagttg tgagttcttc cggcgttcgg ccttctcaac
     3721 ggcattccag agttcggcac ggttcgtaca ccaggcaggg gcggtggcgg gggtcagaat
     3781 cacagcctca gccacaccgc ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc
     3841 aagcatcagt gaaccggaac gataggcagc ggaagctacg gcgctgtagc cgctgcttct
     3901 ggagataatt ttcatgctga gatgataaat cgccatttct gccgtaatgc ctttttgtag
     3961 ttttctttca gccccgcagg gcgcacacaa tcgaaacgga gtggagattg tataagtgcg
     4021 cacttcgtga tttgccgtgc attatgccag ctcagttttt aaactaccag tga
//
