LOCUS       CP056320                4084 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00822 plasmid pRHBSTW-00822_6,
            complete sequence.
ACCESSION   CP056320
VERSION     CP056320.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148703
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00822
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118.141900354428x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 19:50:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,197
            CDSs (total)                      :: 5,082
            Genes (coding)                    :: 4,923
            CDSs (with protein)               :: 4,923
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 64 of 159
            Pseudo Genes (incomplete)         :: 94 of 159
            Pseudo Genes (internal stop)      :: 30 of 159
            Pseudo Genes (multiple problems)  :: 25 of 159
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00822"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00822_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            94..2253
                     /locus_tag="HV286_25975"
     CDS             94..2253
                     /locus_tag="HV286_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLT51869.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(2511..3212)
                     /locus_tag="HV286_25980"
     CDS             complement(2511..3212)
                     /locus_tag="HV286_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLT51870.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3745..3999)
                     /locus_tag="HV286_25985"
     CDS             complement(3745..3999)
                     /locus_tag="HV286_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLT51871.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     Typing          complement(2..93)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1e-40"
     Typing          94..452
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4001..4084)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.8e-36"
     Typing          complement(2512..3212)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8..2250
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cacgaagtgc gcacttatac aatctccact ccgtttcgat tgtgtgcgcc ctgcggggct
       61 gaaagaaaac cacaaaaagg cattacggca gaaatggcga tttatcatct cagcatgaaa
      121 atcatctcca gaaacagcgg ctacagcgcc gttgcttctg ctgcctatcg ttccggttca
      181 ctgatgcttg atgagcgtac cggactgact cacgactaca cccgcaaaag cggtgtggct
      241 gaagctgtga ttctgactcc cgcgacagcc cctgcctggt gtacgaaccg tgccgaactc
      301 tggaatgccg ttgagaaggc cgaacgccgg aagaactcac aacttgcgcg ggaaattgaa
      361 ctcgccatcc cccgcgaact accgcaggat gccgcacggg aaaccgttct cgcctttgtc
      421 agagaaaact ttgtcagtca gggcatgatt gctgatgtgg cgttccatca catggataaa
      481 acaaatcccc atgcgcacat catgctgacc acgagagctg tcggacctgc tggattcggt
      541 ggaaaggtca gggactggaa cgaccggaca catgccgaga cgtggcgtgc atcatgggct
      601 gaccatgcga acagggcact ggcgaacgcc ggttatcagg aagagataga ccatcgttca
      661 tacgagcgac aggggcttga gaaaacgcct ggtattcacc tcggaaagtc tgcctgtgcg
      721 atggaaacac gcggtatcga aacagaacgc ggcgaacaga atcgtctgat taacagactt
      781 aacctggaaa tacagatttc ccgtaccgaa ctcagaaaca gcgggctgac agagcctgcc
      841 cgccgtgtgg ctgaccttct gaatatcgtg cttcctgaca acgcaacttc gtacacacta
      901 cgtgacatca tcgaagcgtt gccgaaggac agtaatgcgg catggaaact caccagtaat
      961 gccatgtcga tgatggcaga tatgcaggcg acgcgcagac gctgggaaga gctgaacacc
     1021 gaacgaaagg aagcgatgag ccatgctgcc agcctgaaaa aatcatcgcc gttttcgtcc
     1081 ggtttttccc gtattcccct gatgcaatgg gcggcaccgg aataccgtag cgaacaggga
     1141 aaaatccagt cgctgagcca gcaaatggaa aaattgcgac agcattaccg gaaggtggag
     1201 aaacaggaca ttcccgcgag tcagacggca tttgaaaaac agtggcagca gtggggggca
     1261 tccggccttg cagctctcag agtccagttg cttaaacgtg aagaggaatt gcgccggaag
     1321 gagcaggagg atacagaacg tagtcgcgcg gagcgacgag cggaacagct caggaaaaat
     1381 gacaatgccg ttatggattc aggggtactg agcgcagtcg ttacggggta tggcgaggcc
     1441 cccatgcctg gtaacgggga aatgacctgt taccttctgc ttcacaaccg gaacggagaa
     1501 tacacgctct gggggaacga gctggagaaa taccgcacca gaatctttga gcgtgttgat
     1561 ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg aaatcggtca acatccacca
     1621 ctgcaacgtg tttattcctc tgcgaccttt gagcaacttc tgacgcaggt ttgccagaca
     1681 tggccgcaat atacccgcaa tctccgccag ccgaagacat ggccggagtc tttctgtctt
     1741 ggagaagacc gccagccagc catgccatca ctggcagcaa ggaaggtgga ttttacgcag
     1801 ggacgtctgc taccgacgct gatgccggta atgagttctg tcgaccggga aacccgccag
     1861 cttcagcttt tgctggtcat gggcgtggat gactctctgg gcggtgttgt ccggcttaat
     1921 ggtacgctgt atcccgcgtt tgccgttccc tcagccgata acagccagct ggtcatcagt
     1981 gcactgaccg ataaggggtt acggtatgca gggtatggtg ttgccgtaaa tcacgatgca
     2041 gacagccaca tatcacctgc gccggaactt atggagtttc atctgaaaac ccgtgaggca
     2101 ccgctttttg ctgccgtcaa cacaccagaa aaacagccgg atcatctgtt ccgctcgctg
     2161 ggttttaatc gtacctggga cgaatggcgg cgcgaggaag atgcacgaac acacgccacc
     2221 gagaggagac atgatcgcgg ttggtcacag tgaatgtgaa caatattttg ttttgtttaa
     2281 catcgtgtta tgttcgtcga tgttcttcga tgttcatcga cagggatgaa tgcaagaagc
     2341 acacacacat aatgcaagca tagttttgtt tttccgcccc caaaaattgg gggttatttt
     2401 ttcgctgcat tagagcgctt ttgacgcaat ttttcacttc aatagtacct tcgcactact
     2461 aacacaaaaa aaacgcagca gggagcgttt taggtgcctt aaaatcgatt ttagatcgtc
     2521 atcttgattt gtttgtcttc cttgaagcga aaatgcaggg ctacaaccgt gcgccccttt
     2581 ttgatcgtct caaccgttac agccaggtcg ctttttttgt tcagttcgtc aatgcagggt
     2641 cttaataatt gctggttaag tgatttatat gtttgatatt tatgctctat atcaagcatt
     2701 gagcgaaaat catcgagagc gataatacgt tcgccagtac tgcggaactg ctggaggagt
     2761 tcgtatatcc ttatactgta acttcttgat aatctagata tattcttgat tacaactcta
     2821 gtaaattgcc cttgtagttg tgtcaaataa ggcattactg cgtcagaaaa tgttatttgt
     2881 gctctggcct cgcctttagc gtactgtgcc cgatactgga tccagcggaa ctcctcacgt
     2941 ttctcgtcat ccttgagaat gattgacctg tcccacaaac gatcaattgc ttcctgtaac
     3001 tgaacctcgg catttttccg ccccatgtca gggaatgaat cgaaatattc cgcagctgta
     3061 atggtcattg tcttttgcaa ttttggagat tctgcatcag ccccagactt caatcgaccg
     3121 atgcaaagta acaaaaatcg ctgttcctgt agggtcaatt tataactggc ttcaaccagc
     3181 gcattggatt tataggcaat tagatccgac atagagcatc cctccccaaa aggtaatgaa
     3241 taaaagataa acataccctc aacgaaaggc aatgcctttt tattttaata tccctttttc
     3301 tgtgaatatt accttaaatc atattgcctt taccttaaat catattacct aaacacctta
     3361 aatcatatta cctttacctt aaatcatatt acctatagcg ttgtaacatt ttgttttcta
     3421 taaggaattt tggcgctaaa acaagtatta aaaacaagat cttaaaaaca agatctaaaa
     3481 acttaacgac gagcctgtgg ataagttgga gtgctgaaaa caaaaaacca ataaaaacaa
     3541 tgcgttgcaa aaaagcagaa ttgcaagttc cgaatacacc ccctccaacc ctgtggaaat
     3601 ccagtattca tgcgggttca gcgcatatca cctcccgaaa aaccgtgcgt atgtgctcaa
     3661 tcaggcgacg gattcacttc cgcttcctcg cccactcgct cgctaacgct cggtcgctgg
     3721 gactgcggcg agcggacgat acaatcactg tcctggcagc gcttccagct cgttaatcag
     3781 cggcagtatg cgcgtcacat cagcctgtcg tgtcacctct gccttcagga actggagtaa
     3841 ccaggcacgc aactgcacct cctccggctt tctgaccttt gccagcaatg ctgcacccac
     3901 aatgaccttc tgcgctgtgt ccagactcct gctcttcgcc ttcaggcgct gtaatctggc
     3961 ctcagcatca gcaatcttct gttcgagtgt tctgctcatg atgtaactcc gtgcgcgatg
     4021 aaaaatcgca ttttagcgcg tcactggtag tttaaaaact gagctggcat aatgcacgga
     4081 aaat
//
