LOCUS       CP056306                4074 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00839 plasmid pRHBSTW-00839_3,
            complete sequence.
ACCESSION   CP056306
VERSION     CP056306.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148710
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00839
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136.822968172424x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 19:41:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,219
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 69 of 175
            Pseudo Genes (incomplete)         :: 115 of 175
            Pseudo Genes (internal stop)      :: 32 of 175
            Pseudo Genes (multiple problems)  :: 37 of 175
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00839"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00839_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2966..4074,1..1051)
                     /locus_tag="HV293_26085"
     CDS             join(2966..4074,1..1051)
                     /locus_tag="HV293_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLT36734.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(1309..2001)
                     /locus_tag="HV293_26090"
     CDS             complement(1309..2001)
                     /locus_tag="HV293_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLT36735.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2533..2787)
                     /locus_tag="HV293_26095"
     CDS             complement(2533..2787)
                     /locus_tag="HV293_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLT36736.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     Typing          complement(2789..2965)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2966..3324
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(1310..2001)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1048
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.427"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2880..4072
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.246"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acaggacatt cccgcgagtc agacggcatt tgaaaaacag tggcagcagt ggggggcatc
       61 cggccttgca gctctcagag tccagttgct taaacgtgaa gaggaattgc gccggaagga
      121 gcaggaggat acagaacgta gtcgcgcgga gcgacgagcg gaacagctca ggaaaaatga
      181 caatgccgtt atggattcag gggtactgag cgcagtcgtt acggggtatg gcgaggcccc
      241 catgcctggt aacggggaaa tgacctgtta ccttctgctt cacaaccgga acggagaata
      301 cacgctctgg gggaacgagc tggagaaata ccgcaccaga atctttgagc gtgttgattt
      361 aatgcgtgac aggagtggtt atatctgtga ccgaagcgaa atcggtcaac atccaccact
      421 gcaacgtgtt tattcctctg cgacctttga gcaacttctg acgcaggttt gccagacatg
      481 gccgcaatat acccgcaatc tccgccagcc gaagacatgg ccggagtctt tctgtcttgg
      541 agaagaccgc cagccagcca tgccatcact ggcagcaagg aaggtggatt ttacgcaggg
      601 acgtctgcca ccgacgctga tgccggtaat gagttctgtc gaccgggaaa cccgccagct
      661 tcagcttttg ctggtcatgg gcgtggatga ctctctgggc ggtgttgtcc ggcttaatgg
      721 tacgctgtat cccgcgtttg ccgttccctc agccgataac agccagctgg tcatcagtgc
      781 actgaccgat aaggggttac ggtatgcagg gtatggtgtt gccgtaaatc acgatgcaga
      841 cagccacata tcacctgcgc cggaacttat ggagtttcat ctgaaaaccc gtgaggcacc
      901 gctttttgct gccgtcaaca caccagaaaa acagccggat catctgttcc gctcgctggg
      961 tttcaatcgt acctgggacg aatggcggcg cgaggaagat gcaagaacac acaccaccga
     1021 gaggagacat gatcgcggtt ggtcacagtg aatgtgaaca atattttgtt ttgtttaaca
     1081 tcgtgttatg ttcgtcgatg ttcttcgatg ttcatcgaca gggatgaatg caagaagcac
     1141 actcacataa tgcaagcata gttttgtttt tccgccccca aaaattgggg gttctttttt
     1201 cgctgcatca gagcgttttt gacgcaattt ttcacttcaa tagtaccttc gcactactaa
     1261 cacaaaaaaa acgcagcagg gagcgtttta ggtgccttaa aatcgatttt agatcgtcat
     1321 cttgatctgt ttgtcttcct tgaagcgaaa atgcagggct acaaccgtgc gccccttttt
     1381 gatcgtctca accgttacag ccaggtcgct ttttttgttc agttcgtcaa cacagggttt
     1441 tattagtatt ttatttaagt ctctgaattg tttgtattta ttttcaatcc ctaacattga
     1501 acgaaagtca ttaagagcaa taattctctc tcccgtactg cggaattgct ggaggaactc
     1561 gtatattctt atactgtaac tacttgataa acctgatatg ttttttataa caacgcgcgt
     1621 gaattgcccc ttcagttgcg tcagataagg cattaccgcg tcagaaaagg ttattcgagc
     1681 cttggcctca cctttagcgt actgtgcccg atactggatc cagcggaact cctcacgctt
     1741 ctcgtcattc ttgagaatga tcgacctgtc ccataaacga tcgatcgctt cctgtaactg
     1801 aacctcggca tttttccgcc ccatgtcagg gaatgaatca aaatattccg cagcggtaat
     1861 ggtcattgtc ttttgcaatt cagcatcagc accagatttc aatcgactga tacacagcaa
     1921 cagaaaacgt tgttcctgta gggtcaactt gtagctggcc tcaaccaaag cattcgactt
     1981 gtaggcaatc aagtccgaca tacaaagatc ccgttcaaat gtggtgatgc tttaatgtgc
     2041 cccacaaata agaaatgtgg aatgtttttt tttatatcct cacatttctg tgaataacac
     2101 cttaaattcc tccaccttta ccttaaattc ctccacctaa acaccttaaa ttcctccacc
     2161 tttaccttaa attcctccac atataccact ataacatatt gattttatta atgaatttca
     2221 cccctaaaac aagtattaaa aacaagatct taaaaacaag atctaaaaac ttaacggcga
     2281 gcctgtggat aacttggggt gctgaaaaca aaaaaccaat aaaaacaatg tgttgcaaga
     2341 aagcagaatt gcaagttccg aatactcccc ttccaaccct gtggaaatcc agtattcatg
     2401 cgagttcagc gcatatcacc tgccgaaaaa ccgtgcgtat gtgctcaacc tggcgacgga
     2461 ttcacttccg cttcctcgcc cactcgctcg ctaacgctcg gtcgctgggg ctgcggcgag
     2521 cggacgatac aatcactgtc ctggcagcgc ctccagctcg ttaatcagcg gcagtatgcg
     2581 cgtcacatca gcctgtcgtg tcacctctgc cttcaggaac tggagtaacc aggcacgcaa
     2641 ctgcacctcc tccggctttc tgacctttgc cagcaatgct gcacccacaa caaccttctg
     2701 cgctgtgtcc aggctccggc tcttcgcctt caggcgctgt aatctcgcct cagcatcagc
     2761 aatcttctgt tcgagcgttc tgctcatgat gtaactccgt gcgcgatgaa aaatcgcatt
     2821 ttagcgcgtc actggtagtt taaaaactga gctggcataa tgcacggcaa atcacgaagt
     2881 gcgcacttat acaatctcca ctccgtttcg attgtgtgcg ccctgcgggg ctgaaagaaa
     2941 actacaaaaa ggcattacgg cagaaatggc gatttatcat ctcagcatga aaattatctc
     3001 cagaagcagc ggctacagcg ccgtagcttc cgctgcctat cgttccggtt cactaatgct
     3061 tgatgagcgt accggactga ctcacgacta cacccgcaaa agcggtgtgg ctgaagctgt
     3121 gattctgacc cccgcgacag cccctgcctg gtgtacgaac cgtgccgaac tctggaatgc
     3181 cgttgagaag gccgaacgcc ggaagaactc acaacttgcg cgggaaattg aactcgctat
     3241 cccccgtgaa ctgccgcagg atgccgcacg ggaaaccgtt ctcgcctttg tcagagaaaa
     3301 ctttgtcagt cagggcatga ttgctgatgt ggcgttccat cacatggata aaacaaatcc
     3361 ccatgcgcac atcatgctga ccacgagggc tgtcggacct gctgggttcg gcggaaaggt
     3421 cagggactgg aacgaccgga cacatgccga gacgtggcgt gcatcatggg ctgaccatgc
     3481 aaacagagca ctggcgaacg ccggttatca ggaagagata gaccatcgtt catacgagcg
     3541 acaggggctt gagaaaacgc ctggtattca cctcggaaag tctgcctgtg cgatggaaac
     3601 acgcggtatc gaaacagaac gcggcgaaca gaatcgtctg attaacaggc ttaacctgga
     3661 aatacagatt tcccgcactg aactcaggaa cagcgggctg acagagcctg cccgccgtgt
     3721 ggctgacctt ctgaatatcg tgcttcctga caacgcaact tcgtacacac tacgtgacat
     3781 catcgaagcg ttgccgaagg acagtaatgc ggcatggaaa ctcaccagta atgccatgtc
     3841 gatgatggca gatatgcagg cgacgcgcag acgctgggaa gagctgaaca ccgaacgaaa
     3901 ggaagcgatg agccatgctg ccagcctgaa aaaatcatcg ccgttttcgt ccggtttttc
     3961 ccgtattccc ctgatgcaat gggcggcacc ggaataccgt agcgaacagg gaaaaatcca
     4021 gtcgctgagc cagcaaatgg aaaaattgcg acagcattac cggaaggtgg agaa
//
