LOCUS       CP056296                1488 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Citrobacter freundii strain RHBSTW-00857 plasmid pRHBSTW-00857_4,
            complete sequence.
ACCESSION   CP056296
VERSION     CP056296.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148717
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00857
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 161.140534995879x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:07:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,792
            CDSs (total)                      :: 4,677
            Genes (coding)                    :: 4,576
            CDSs (with protein)               :: 4,576
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 36 of 101
            Pseudo Genes (incomplete)         :: 66 of 101
            Pseudo Genes (internal stop)      :: 19 of 101
            Pseudo Genes (multiple problems)  :: 17 of 101
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00857"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00857_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(345..869)
                     /locus_tag="HV300_23960"
     CDS             complement(345..869)
                     /locus_tag="HV300_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="QLT06857.1"
                     /translation="MLVNTEGQANQRLKELEKIKNKASVEHEEAKKNRNFTQMYPKGWQ
                     RVRELLKDKQGISAIQLYSFLAEHIDPTCGAVVCDQQFLAEKMDVSTRTIRRWLTYLEG
                     KNALVRIPVAGKVCAYALDPHEVWKGYDTSKEYAAFVTKTLVNKDGEIQRRIMAMFSGT
                     ESGQEQKRSDS"
                     /name="DeoR family transcriptional regulator"
ORIGIN
        1 gtattttgct gtcacgacgc caacaggcat aaggtttgaa gttgtgtgat gagtcataca
       61 acgaccgata agcttttttg gttttgtttt tggggtttcg gggcgcagcc ctgaaccagt
      121 cacgacgcgc tagcggtgtg tatactggct tacctgtatt ttggcatcgc agatcggaca
      181 gtttcgaaga aactactaag tgcgcatttg aaacttttga attcgtattg gatgtgtttg
      241 tactagggga tagaggctca agaaatacca aacccgctta tcagggggat ggctcgcagt
      301 ggcgaaccat cccccatatc aaggatgggg gtcacttcaa gccgtcaaga gtcgcttcgc
      361 ttttgctctt gaccgctttc cgttcctgaa aacatggcca tgatacgacg ttgaatttca
      421 ccatctttat tgaccaaggt tttagttacg aaagcagcat attccttaga tgtatcataa
      481 cctttccaca cctcgtgagg gtctaaagca tatgcgcaaa ccttgccagc aactggtatt
      541 ctaactaacg cattttttcc ttcgaggtac gtcaaccaac gacgaatagt tcttgtcgag
      601 acgtccattt tttccgctaa gaattgctgg tcacaaacta cagctccgca tgttggatct
      661 atatgttcag ctaaaaaaga gtaaagctgg atagcggaaa taccctgctt gtctttcaga
      721 agctctctaa cgcgttgcca gccctttgga tacatctggg tgaagtttct gttcttcttc
      781 gcttcctcat gctctacaga cgccttattt ttgatttttt ctaattcttt gagcctttga
      841 ttggcctgtc cttcagtgtt aaccaacatt cgagatctcc catcttgatt tcgtgacctc
      901 atgataggtc atttttgtcc gattgtaggc caaaacgcgt cctacgtgaa gacatgagcg
      961 gacatccagg gtgtccggat gagcggacac gcagggtgtc cgcatagaaa aaataaaatt
     1021 aaataaaatc aatttgttat taatttgccc ttttctattc tttgaaaacc taaaggcaac
     1081 accaaaattc aaaaccacga aagtagctcc ctcccttcgg tcgggcattt ttggggtcaa
     1141 ggttcgcgtt agcgaccgcg aagcggcttg acctttacgc caaaaattcc cgttcacact
     1201 tgtgagtggt ggttggtttc acgaagtgag gccaaccacc tgcaaaagct attttttaaa
     1261 gttcatgacg atcacgatta cgtcgataat aattttggcg atttccagtg taagtttcat
     1321 ttaaagccct ttaatgattt tgttgtggtt ccggcagttg attgtttatg gctacaaaag
     1381 tcgagaaaca gtgccagagg tacaaatgcc gcaacatcga atcgaagtta gatttacacg
     1441 tatccattcc attgataatg ctgatggagt tcagtgctat tcaccttt
//
