LOCUS       CP056292                1488 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Citrobacter freundii strain RHBSTW-00858 plasmid pRHBSTW-00858_4,
            complete sequence.
ACCESSION   CP056292
VERSION     CP056292.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148718
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00858
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 165.36211553509x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:04:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,792
            CDSs (total)                      :: 4,677
            Genes (coding)                    :: 4,574
            CDSs (with protein)               :: 4,574
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 37 of 103
            Pseudo Genes (incomplete)         :: 66 of 103
            Pseudo Genes (internal stop)      :: 20 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00858"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00858_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(140..664)
                     /locus_tag="HV301_23960"
     CDS             complement(140..664)
                     /locus_tag="HV301_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="QLT02281.1"
                     /translation="MLVNTEGQANQRLKELEKIKNKASVEHEEAKKNRNFTQMYPKGWQ
                     RVRELLKDKQGISAIQLYSFLAEHIDPTCGAVVCDQQFLAEKMDVSTRTIRRWLTYLEG
                     KNALVRIPVAGKVCAYALDPHEVWKGYDTSKEYAAFVTKTLVNKDGEIQRRIMAMFSGT
                     ESGQEQKRSDS"
                     /name="DeoR family transcriptional regulator"
ORIGIN
        1 atttgaaact tttgaattcg tattggatgt gtttgtacta ggggatagag gctcaagaaa
       61 taccaaaccc gcttatcagg gggatggctc gcagtggcga accatccccc atatcaagga
      121 tgggggtcac ttcaagccgt caagagtcgc ttcgcttttg ctcttgaccg ctttccgttc
      181 ctgaaaacat ggccatgata cgacgttgaa tttcaccatc tttattgacc aaggttttag
      241 ttacgaaagc agcatattcc ttagatgtat cataaccttt ccacacctcg tgagggtcta
      301 aagcatatgc gcaaaccttg ccagcaactg gtattctaac taacgcattt tttccttcga
      361 ggtacgtcaa ccaacgacga atagttcttg tcgagacgtc cattttttcc gctaagaatt
      421 gctggtcaca aactacagct ccgcatgttg gatctatatg ttcagctaaa aaagagtaaa
      481 gctggatagc ggaaataccc tgcttgtctt tcagaagctc tctaacgcgt tgccagccct
      541 ttggatacat ctgggtgaag tttctgttct tcttcgcttc ctcatgctct acagacgcct
      601 tatttttgat tttttctaat tctttgagcc tttgattggc ctgtccttca gtgttaacca
      661 acattcgaga tctcccatct tgatttcgtg acctcatgat aggtcatttt tgtccgattg
      721 taggccaaaa cgcgtcctac gtgaagacat gagcggacat ccagggtgtc cggatgagcg
      781 gacacgcagg gtgtccgcat agaaaaaata aaattaaata aaatcaattt gttattaatt
      841 tgcccttttc tattctttga aaacctaaag gcaacaccaa aattcaaaac cacgaaagta
      901 gctccctccc ttcggtcggg catttttggg gtcaaggttc gcgttagcga ccgcgaagcg
      961 gcttgacctt tacgccaaaa attcccgttc acacttgtga gtggtggttg gtttcacgaa
     1021 gtgaggccaa ccacctgcaa aagctatttt ttaaagttca tgacgatcac gattacgtcg
     1081 ataataattt tggcgatttc cagtgtaagt ttcatttaaa gccctttaat gattttgttg
     1141 tggttccggc agttgattgt ttatggctac aaaagtcgag aaacagtgcc agaggtacaa
     1201 atgccgcaac atcgaatcga agttagattt acacgtatcc attccattga taatgctgat
     1261 ggagttcagt gctattcacc tttgtatttt gctgtcacga cgccaacagg cataaggttt
     1321 gaagttgtgt gatgagtcat acaacgaccg ataagctttt ttggttttgt ttttggggtt
     1381 tcggggcgca gccctgaacc agtcacgacg cgctagcggt gtgtatactg gcttacctgt
     1441 attttggcat cgcagatcgg acagtttcga agaaactact aagtgcgc
//
