LOCUS       CP056179                4096 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00685 plasmid
            pRHBSTW-00685_6, complete sequence.
ACCESSION   CP056179
VERSION     CP056179.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148683
KEYWORDS    .
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00685
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 81.2704466984096x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:41:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 4,995
            Genes (coding)                    :: 4,894
            CDSs (with protein)               :: 4,894
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 30 of 101
            Pseudo Genes (incomplete)         :: 56 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 11 of 101
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00685"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00685_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(6..185)
                     /locus_tag="HV266_25545"
                     /pseudo=""
     CDS             complement(6..185)
                     /locus_tag="HV266_25545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(337..660)
                     /locus_tag="HV266_25550"
     CDS             complement(337..660)
                     /locus_tag="HV266_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLU94927.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1694..1963)
                     /locus_tag="HV266_25555"
     CDS             complement(1694..1963)
                     /locus_tag="HV266_25555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU94922.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            2045..2278
                     /locus_tag="HV266_25560"
     CDS             2045..2278
                     /locus_tag="HV266_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QLU94923.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2300..2527
                     /locus_tag="HV266_25565"
     CDS             2300..2527
                     /locus_tag="HV266_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QLU94924.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(3057..3266)
                     /gene="mbeD"
                     /locus_tag="HV266_25570"
     CDS             complement(3057..3266)
                     /gene="mbeD"
                     /locus_tag="HV266_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLU94925.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="mbeD"
     gene            complement(3270..3755)
                     /locus_tag="HV266_25575"
     CDS             complement(3270..3755)
                     /locus_tag="HV266_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLU94926.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          complement(769..847)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          923..1411
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(4..347)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.391"
                     /coverage="81.0"
                     /evalue="1.34e-65"
     Typing          complement(2948..4095)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.739"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtcagcagct ccgtgttcgg gatcaggaag ttcagctcca gccgcccctt gtcccggtgc
       61 tcaacccaca gcacgctgta ctggtcttta tcgagtccgg gcatcagaac ccgctcgaag
      121 ctcgccatca gcgtttcacg ctgcccgggc ggtaagtcct gttcggcaaa ggacagcacc
      181 ccggaggtgt actttttggc gtagggcgag gcgtcgataa gctcccggac ttcctccggc
      241 ttcccctgaa gaacgctggc accgtcgcgc tggcggtctt tccccagcag ataatcaacc
      301 gggccggcac cgcctccgcg tccccgggga tgaaacttaa cgatcatcgt cagccccctt
      361 ttccagtacg gcatgccgca gccgctcgag tccggcatcg atggccatca gcgcggcgac
      421 gacctgcacg cggtcgagac cgctactgcc accggcgtta acctggcggg caatctggtt
      481 gaggttgttc cccatgccgg caagctgacg aagcagcgcc ggcgagatcg agggaagttt
      541 gccggcccgg gcgggcttct cgtccaggca cgtctgccgc atccaggccg cgagctgttt
      601 accgtcgcag cgttccagca gacggcggtg ctcatcttct gttacccaca tcgtgagcat
      661 tttgctgcgt ttgtctgcca atccggcctc ctgataacgc cgggacgggc gggaaatctt
      721 ttcccggtcg gcgcggtgtt gggcaagccg caggcgcgtc agtggcattt agcgggtttc
      781 ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc ttactatggt
      841 ggcacgacgg gaatcgggat gaaatagtca gtcatgaaaa ctgtgagatg aggctcaggt
      901 gcgtaagagg ctgatgcctg tcccggtgga tattccgctt cctcgctcac tgactcgcta
      961 cgctcggtcg ttcggctgcg gcgagcggta cggactcacg gataaagacg tggcggggaa
     1021 atacgtgaat gcaggagggg aagaaaggat gcgccgctgc cggttttcca taggctccgc
     1081 ccccctgaca agcatcacag aatctgacgc tcaaatcagt ggtggcgaaa cccgatgcgg
     1141 cttaaagata ccaggcgttt ccccctgatg gctccctcgt gctcccccgc attcctgcct
     1201 ttcggtttac cggtgtcatt ccgctgttat ggccgcggtt tctcattcca cgcctgacac
     1261 tcagttccgc gaaggcagtt cgctccagga ttgactgtat gcacgaaccc cccgttcagt
     1321 ccgactgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacacgaaa
     1381 aatcgctact ggcagcagcc attagtaact ggaggtatac gagagacaaa ttaagaggtc
     1441 ttgaagtatg ggcctgagag aggctacact gaaagaacag ttttggtttc tgcggtctcg
     1501 tatacccagt tacctggtta agcagttccc caactgactt aaccttcgat caaaccacct
     1561 ccccaggtgg ttttttcgtt ttcagagcaa gagattacga ctgtaacgac aggatcacaa
     1621 gaagatcatt tactcagaga gactaactac cactatgttt gcctatcaac atacttagga
     1681 aacattactt gagttaacga ctccacctta cttttagtat ctcgcaatga atcttccgac
     1741 cgccgttgtg ttgaagttct attgcgccac cggggtataa atcactttcg tttaactcaa
     1801 ttaaaagctc gttcttgtga gcatcctcta aataaaggta ataacaccca ccataaaggt
     1861 tctgcgtatc tggcacggca acaaaagatt tctttccgaa cagtaaaaca gcctcttttt
     1921 ctccatgctc tgtcagcaat aatcgaaatt cttgtccatt caatcctctt ttcgcatgaa
     1981 aaaacgacct atacttagat ggttagtgac catatccact ttacctttaa gcagggaacc
     2041 tattatgaat aaaaaaacgc tgttggcggc catgatggtc gcgttggtga cctttgccgc
     2101 tgctggatgt tcttcaaacc aagaaataaa aaccacagat gatcgcacta tcgttacaga
     2161 cggcaaaccc aaagttgaca gtgatacggg attagtatcc tacaaagatg cccatacagg
     2221 ccgaaccgaa cagatcaacc gtgacaaaat taccaacatg agtgaactag acaactgata
     2281 cggataaaag gagctggctt tgaaaaagat ttttgtttca gtgttcgcag cggcagttgc
     2341 gctttcagcc ctaaccggct gcacgcgcac aagctatgcc atccacacca acgacgggcg
     2401 tacaattgtc agcgacggca agcctacaga atcagactca ggcctgctgg gctataaaga
     2461 cgcaaatggt gtcaaacagc agatcaataa agccgacgtt aaagaagtct ctgaaattcc
     2521 gcattgaggc atcgctctct ccgaaaaggg ccgctaatgc ggcccttttc tttatctgcc
     2581 ccaacaacag gcaaaaaaag cccccgctgg gggccaaata cctgcagtgg ctggatagta
     2641 ctgcattcct gatgaaggag acgaataagt atgggccgga tttttgcgtc tgcaggctcg
     2701 gtcgccacaa tttttccgta cccgccaggc atcatgcttt taccataaca tcccttgccc
     2761 gcacctctcc ggacgggttc agcgcgaatg cggcgtcagt cctgcaaaac cgacagtaaa
     2821 ccaccccgca aaccgtgcat tctgcgcctg ataaatctca cccgactgca tagccatgca
     2881 gccgggtgaa tggcaccggg tttcggcgca gatcctcaat gaaagccccg ttttatccgg
     2941 ttatcacagc gacggaccgt catactcctg ctgacgctgc gatcgttcca gctccagcgc
     3001 tttctggtgc tgcgctgcgc gctcctgctc ccggtgtgcc tcgatgtcgt tcagccttac
     3061 gacaaccggc gcagctgctc gcttaaactc agcacctgcg tgctcaagtc cgtgacgcgc
     3121 tcgctcagcg ctgcgttctc ccgttgcata agaccacaca tcgtccgcca ttccgcgaag
     3181 gcgctctccc actcgtccag ccttttcgag tagtcctgct gtagctgctc taatgcgctc
     3241 agcaactgtt tttccagttc cgtcatgtgc tatttccccg ccagtatcat ccagctcgaa
     3301 tcctctccga accgtcccgc gtccgggttc accctcacgc agcggcgtcg ttcctctccg
     3361 caggtcgtga gctgcacgcc gctgttcctc tcggacagca tggcctgcgt gctcttctgc
     3421 tcccggatgg tcgtgtaatt gtcgagtatc tgctgcccct gccaccatag aatgcccgca
     3481 cttgaggcta tcagcagcac ggagaccagg acgatggtca gccacgtctg gctgaccatg
     3541 cgcagcatcc ctttcgtacg ctggctcatg gctgaggaca gcatccggtc gtggtcgtgg
     3601 atggcggcgc tgattctctt ctcgctttcg ttcagttccg ctctgacaga cttctcgtgc
     3661 tcgctgaatg cggctttcag catctcgccg gtagtctgct gctgcacttt cgatttctgc
     3721 tctaagtcct tcgccagcgt taaaagactg ttcatagatg gctcccttca gtcggatgtt
     3781 tcgccccccg tccgggtcgg caatgctgat gctgcttttt gtggtgcgaa ccacctcaaa
     3841 acctgcgctt tccagcgcct cagtgacgtc ctgacgcgtt ttcagctccc cggacgaggc
     3901 taaggccagt aaaccccgcg taatcgcctg agcggcttcc tgcttcgttt ccggcagcgc
     3961 ggacggcgtg accagtgccc gccggttttc cggcgcgttc gggtcgtgca gccccagact
     4021 gccgttcacg atggtctgcc aggcatcaat gcgaggacgg tcggcgcggt catagtatgg
     4081 ctggagccgt ttgccg
//
