LOCUS       CP056178                4858 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00685 plasmid
            pRHBSTW-00685_5, complete sequence.
ACCESSION   CP056178
VERSION     CP056178.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148683
KEYWORDS    .
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00685
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 81.2704466984096x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:41:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 4,995
            Genes (coding)                    :: 4,894
            CDSs (with protein)               :: 4,894
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 30 of 101
            Pseudo Genes (incomplete)         :: 56 of 101
            Pseudo Genes (internal stop)      :: 26 of 101
            Pseudo Genes (multiple problems)  :: 11 of 101
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00685"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00685_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4485..4858,1..55)
                     /locus_tag="HV266_25510"
     CDS             join(4485..4858,1..55)
                     /locus_tag="HV266_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU94921.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            1038..1361
                     /locus_tag="HV266_25515"
     CDS             1038..1361
                     /locus_tag="HV266_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLU94916.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1300..2064
                     /locus_tag="HV266_25520"
                     /pseudo=""
     CDS             1300..2064
                     /locus_tag="HV266_25520"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2039..2530
                     /locus_tag="HV266_25525"
     CDS             2039..2530
                     /locus_tag="HV266_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLU94917.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2537..2752
                     /gene="mbeD"
                     /locus_tag="HV266_25530"
     CDS             2537..2752
                     /gene="mbeD"
                     /locus_tag="HV266_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLU94918.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="mbeD"
     gene            3224..3715
                     /locus_tag="HV266_25535"
     CDS             3224..3715
                     /locus_tag="HV266_25535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU94919.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(3772..4200)
                     /locus_tag="HV266_25540"
     CDS             complement(3772..4200)
                     /locus_tag="HV266_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLU94920.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     Typing          752..925
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(283..828)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          1352..2844
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actgttagag cttgggtatc atgaccctat gcaactcccc caaaatttcg aatgatctct
       61 tgttcggatg aatgatccct tgagattcgt tgaccctcgt cgtaatctct tgcggtgaaa
      121 acgaaaaaac cacccggcaa ggtggctttt tcgaaggttc gcaagagttg acgctctttt
      181 gaaccgcggt aactggcttg gaggagcaca gtcaccaaaa ctgtcctttc agtgtagcct
      241 tatcgcaccc gaaacttcaa gactctcctc ctctaaacct gttaccagtg gctgctgcca
      301 gtggcgcttt gtcgtgtttt tccggattgg actcaagttg atagttaccg gataaggcgc
      361 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttcc
      421 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
      481 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg
      541 tatctttaag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
      601 tgtcaggggg gcggagccta tggaaaaacg gcttcgccgc ggccttatcg tttccctgtt
      661 aagtttcttc ctggcatcta ccaggaattt ccgccccatt cgtaagccca caccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaata tatcctgtat
      781 cgcatattct gctgacgcgc cggtgccgcc ttttttctcc tgccacatga agcacttcac
      841 tgattcccgc acccgtgcca atataataag ccagtataca ctccgctagc gctacgtgac
      901 tggttcaggg ctgcgccccg aaacccgcca aaagcctgac gcgcctgcgg cttgtccaac
      961 cccgcgccga ccgggaaagg ctttcccgcc cgtcccggcg ttatcaggag gccggtttgt
     1021 cagagaaacg gagcaagatg ctcaccatgt gggtgacgga ggacgagcac cggcggctgc
     1081 ttgagcgctg tgacggcagg cagctggcgg cgtggatgcg gcagatctgc ctggacgaaa
     1141 agccggcacg ttccggaaag ctcccctcga tctcgccggc gctgctgcgc cagcttgccg
     1201 gcatgggcaa taacctgaac cagatagccc gccgtgttaa cgccggcggc ggcaccggcc
     1261 atgacagggt acagattgtg gcggcgctga tggccatcga tgccggcctt gagcggctgc
     1321 ggcacgccgt tcttgagaaa ggaacggacg atgatcgtta aatttcatcc gcgcggccgc
     1381 ggcggtggcg ccgggccggt ggactatctg ctgggtaaag accggcagcg tgaaggcgcc
     1441 accgtcctgc aggggaagcc ggaagaagtc cgggagctga ttgacgcgtc gccttacgtt
     1501 aagaaataca cctccggcgt tctgtcgttt gcggaggccg atctgcctcc cggccagcgg
     1561 gaaaaactga tggccagctt cgagcgggtt ctgatgcccg gactcgataa agatcagtac
     1621 agcattctgt gggtggagca cgcggacaag gggcgtctgg agctgaactt tctgatcccc
     1681 aataccgagc tgctgaccgg caggcggctc cagccttact acgaccgcgc cgaccggccg
     1741 cgcatcgatg cctggcagac tatcgtgaac ggcagactgg ggctgcacga cccgaacgcg
     1801 ccggaaaacc gccgggcgct ggtcacgccg tccgggctgc cgaaaacgaa gcaggaggcc
     1861 gcagaagcca ttacccgggg actgctgacc cttgcttcgt ccggggagct gaaaagccgt
     1921 caggatgtca ctgacactct tgaaggtgca ggttttgagg tggtgcgcac cacgaaaagc
     1981 agtatcagca ttgccgaccc ggacgggggg cgaaacatcc gactgaaggg agccatctat
     2041 gagcagtctt ttaacgctgg cgaaggactt agagcagcaa tcgaaagcgc agcagcagag
     2101 taccggcgag atgctgaaag ccgcattcag cgagcacgag cagtctgtca gagcggaact
     2161 gagcgcaagc gcgaggagaa tcagcgacgc catcctcgcc cacgagcaga gtatgagcga
     2221 ggcgatggag aaaaaccggc ggagcgtact gcggactgcc gggcggacat ggctcaccat
     2281 cctgatggtg tcagcgctgc tgatcggcac gagcgggagc attctgtggt ggcaggggca
     2341 gcagataacc gacaattaca cgcatcttcg tcagcaggaa gataccctgg cgaaaatgac
     2401 cgcccggacg tggggagtgc gctatcagga gagcagcgac ggccggaggt ttcttatcct
     2461 gccgccgggg atgcaaaccg aagccatccc ctacgacggg acgacatgga tccgcctgaa
     2521 acaggagtag cggaaaatga cagagctgga aaagcagttg ctgagcgcat tagagcagct
     2581 acagcaggac tactcgcaaa ggctggacga atgggagagc gccttcgcgg aatggcggag
     2641 catgtgtggt cttatgcaac gggagaacgc gatgctgaac gagcgcgtgt cggacttgag
     2701 cacgcaggtg ctgagtttaa gcgagcagct gcgccgcttg tccgggaact gaatgccatc
     2761 gagctccggc agagggagaa tgagtatcag aaaacactgg agctggagcg ccagccgcag
     2821 aaaacctacc gcgggccgac actttaaacc atccaggcag ctgacaaccc gcacgaagag
     2881 gggctcttat ttctcccgct aaataggttc gtgatcaaaa aggtcacgac ttattcagga
     2941 gcgggaaatg aagctgttaa atcttattga atcattgctt aactgcctgt atctggcggc
     3001 ggagctgtgg gactggctgg agaagcattt gtcggttttt aaggcattgg cgcggatttt
     3061 agtgtgactt taacatccat gcatctggct gcatgactat gcaccagtgt aatattcagc
     3121 ggctgcgttt ctggtgtttt ttcgttatgt ctgtcgccac tttctcgcgt ctggcaccgg
     3181 aaacgcagcc aaccccgttt cagcggtgcg ggcaacagat gttatggtaa atcaaccgtc
     3241 cgttgataaa ggttctgata atacccatgt aggtgatgtt gtttgctttt tatctacaca
     3301 ctgcctgatg gagctattta tgaatgctga tgaagttaca cgaattcttg cgcaatcgag
     3361 tcaaaatgat tggattgtgg atgatgagac aggggcgttt acttataaaa atgatttagc
     3421 actgcacatt aagagagctg attacgacga ctacaactcg tttaatgagc cttgggctgt
     3481 tcgtcatcct gatagtaatg cgtgttcagt cgagtacacc gttcactatg gttcttcgtt
     3541 tgttgataga aaaaccttgg tttccgtcga tggtcatcgt gcaaccttgc ctatgcctgc
     3601 atccgcaacg gatctaaacg tgtcatcggc tgatgtgaat ttcgccaaaa ttgtaaacat
     3661 tggcgatcgc gttgatgaat acattcaacg ttctggtctt acgattaagc cctaatttaa
     3721 cgataagccc ggcctccaaa tgcgggctgt tcctgcatgt aaccagcata actaatcggc
     3781 ttttttcctt actgtatata ttggcttttt atgtcgcggc tttcttactt gctcggacac
     3841 agaaggacca attatgtcta aataatctac aagctttgta aggtcgtata tgttgcggcc
     3901 accatatcgg ttgtcgcttt ttgatgtcgg ttttttatct aagagattgg ctttttctag
     3961 gtcattaata gccctttgca ccgtccttac tgacaccccc atccgtatag ccaggctctc
     4021 cattgagggg tgcggccact cctgtagatt ccaccagtgt actatgagat tcagcaaggt
     4081 attgaatgct acagggctca atttcttctc attttgtata aaaaacaaca cagtaggtac
     4141 tgcagtccat cctaatgaca gacctccagc cttccatttt ttttctattg cgtaactcat
     4201 tgattatcct gtaaatattt tttttgagat atatataata taagtattca tatgaatagg
     4261 gtgacaccag cgccattatt acacaactat aaaaggctaa tctgagaaca gaaaaacatt
     4321 tctatctgca ttaaatacgg agttagtgat atgaaaacca aacaacatca tgatcttgta
     4381 aaagatactg aaatatttgg tgtatccttg tctctgttga tttccagagc gaatgagaga
     4441 tctgttacaa tgccatctgg tctaagtagg gagcagagaa gagcatgggc agcggatcaa
     4501 gcacgcaaaa tccacaattg aatgttgtct ctttctctga gacattcaat ctttattaca
     4561 catcccgctt ttctcaaaaa acactcgata aaatcgatga cttcatcgaa catttcgaga
     4621 agtacaatct ttttggcgat gccttaaaag gccatccggc ttggattggc aaggtatcgc
     4681 catcttggaa cgtgcctgac agttacgaaa acaaagaagc tattgaagaa catgccaggg
     4741 ctaataaatt gtggcatgcc catataggcg atcccgtttt catcgataca tttcacggga
     4801 agtacaaagt ctctgattgg gtcatccact tccaacgtct agcgccgaac catataaa
//
