LOCUS       CP056028               13841 bp    DNA     circular BCT 28-JUN-2020
DEFINITION  Klebsiella pneumoniae strain 39428 plasmid pKPN39428.4, complete
            sequence.
ACCESSION   CP056028
VERSION     CP056028.1
DBLINK      BioProject: PRJNA633552
            BioSample: SAMN14973061
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Satlin,M.J., Chen,L., Patel,G., Gomez-Simmonds,A., Weston,G.,
            Kim,A.C., Seo,S.K., Rosenthal,M.E., Sperber,S.J., Jenkins,S.G.,
            Hamula,C.L., Uhlemann,A.C., Levi,M.H., Fries,B.C., Tang,Y.W.,
            Juretschko,S., Rojtman,A.D., Hong,T., Mathema,B., Jacobs,M.R.,
            Walsh,T.J., Bonomo,R.A. and Kreiswirth,B.N.
  TITLE     Multicenter Clinical and Molecular Epidemiological Analysis of
            Bacteremia Due to Carbapenem-Resistant Enterobacteriaceae (CRE) in
            the CRE Epicenter of the United States
  JOURNAL   Antimicrob. Agents Chemother. 61 (4), e02349-16 (2017)
   PUBMED   28167547
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  AUTHORS   Michalik-Provasek,J., Carolina-Hernandez,A., Satlin,M., Chen,L.,
            Kreiswirth,B., Walsh,T., Zagaliotis,P., Hillhouse,A. and Gill,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Microbial Pathogenesis & Immunology, Texas
            A&M Center for Phage Technology, 300 Olsen Blvd, College Station, TX
            77843, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2020
            Assembly Method                   :: Unicycler v. 0.4.6.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 194.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/22/2020 14:15:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,684
            CDSs (total)                      :: 5,563
            Genes (coding)                    :: 5,467
            CDSs (with protein)               :: 5,467
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 55 of 96
            Pseudo Genes (internal stop)      :: 14 of 96
            Pseudo Genes (multiple problems)  :: 13 of 96
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Dr. Jason Gill, Center for
            Phage Technology Klebsiella Strain Collection. Assembled with the
            help of reads in SRR5168842 experiment number SRX2485638.  Used
            with permission of Liang Chen and Barry Kreiswirth.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="39428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN39428.4"
                     /geo_loc_name="USA"
                     /collection_date="2013"
                     /collected_by="Barry Kreiswirth"
     gene            complement(join(13614..13841,1..1737))
                     /locus_tag="HPS03_02055"
     CDS             complement(join(13614..13841,1..1737))
                     /locus_tag="HPS03_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QKY97178.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3218..3367)
                     /locus_tag="HPS03_02060"
     CDS             complement(3218..3367)
                     /locus_tag="HPS03_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QKY97166.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3452..3709)
                     /locus_tag="HPS03_02065"
     CDS             complement(3452..3709)
                     /locus_tag="HPS03_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QKY97167.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            complement(3719..4540)
                     /locus_tag="HPS03_02070"
     CDS             complement(3719..4540)
                     /locus_tag="HPS03_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxic family protein"
                     /protein_id="QKY97168.1"
                     /translation="MSFRSTERQTPTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDK
                     AKAELDAEDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEY
                     GSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKD
                     AENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYG
                     DKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cytotoxic family protein"
     gene            complement(4656..4826)
                     /locus_tag="HPS03_02075"
                     /pseudo=""
     CDS             complement(4656..4826)
                     /locus_tag="HPS03_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            complement(4881..5585)
                     /locus_tag="HPS03_02080"
     CDS             complement(4881..5585)
                     /locus_tag="HPS03_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="QKY97169.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(5645..5866)
                     /locus_tag="HPS03_02085"
                     /pseudo=""
     CDS             complement(5645..5866)
                     /locus_tag="HPS03_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206318.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            complement(5936..6490)
                     /gene="aac(6')-Ib"
                     /locus_tag="HPS03_02090"
     ARG             complement(5940..6490)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6724..7281)
                     /locus_tag="HPS03_02095"
     CDS             complement(6724..7281)
                     /locus_tag="HPS03_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QKY97170.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            7443..10448
                     /locus_tag="HPS03_02100"
     CDS             7443..10448
                     /locus_tag="HPS03_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="QKY97171.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            10483..10773
                     /gene="exc2"
                     /locus_tag="HPS03_02105"
                     /pseudo=""
     CDS             10483..10773
                     /gene="exc2"
                     /locus_tag="HPS03_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="exc2"
     gene            11079..11342
                     /locus_tag="HPS03_02110"
     CDS             11079..11342
                     /locus_tag="HPS03_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QKY97172.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            11332..11631
                     /locus_tag="HPS03_02115"
     CDS             11332..11631
                     /locus_tag="HPS03_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QKY97173.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(11693..12070)
                     /locus_tag="HPS03_02120"
     CDS             complement(11693..12070)
                     /locus_tag="HPS03_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QKY97174.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            12139..12318
                     /locus_tag="HPS03_02125"
     CDS             12139..12318
                     /locus_tag="HPS03_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QKY97175.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(12346..12876)
                     /locus_tag="HPS03_02130"
     CDS             complement(12346..12876)
                     /locus_tag="HPS03_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKY97176.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(12883..13614)
                     /locus_tag="HPS03_02135"
     CDS             complement(12883..13614)
                     /locus_tag="HPS03_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QKY97177.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     ARG             complement(5598..5854)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             complement(5937..6541)
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          2537..3115
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(12887..13614)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 ccgcgcctcc ttctggatga agctgtcccc gctgatatgc tccacgttcc ccgcctcccg
       61 gctggcacgg cggcgctcca cgtccaccct gatttccccg gtctgccccg ccgcccactg
      121 cgcggactcc aggctggccg cccggtaaaa ccagcggatc ggcgtgttat ccagtaccgt
      181 ggttttcaca aattcagcgg gcagatcctg cccgcactgc ccgaaatcgc ccagcgactg
      241 gtgcgcgagc aacagattgc agtcccggct gcgcagcgtc cccagtgcgt tcagtacata
      301 cttcgacagc agatatttaa tttcatcgag catcacgctg acgtgtggcc actcccggaa
      361 ctcgtcccgc gcaatgatga tttgcgcgca gcgggcaaac agcatttttt gcacacggat
      421 caccgcctcg tcgtccatcg agccgataac gtacaggcag ccgccgccgt tgataatccc
      481 cgccacatcg atgccactgt ccgtctgcag gacgctcagg tcagcgacgt tttccagcct
      541 agtgatcaac cccttcacgt cctttttcag ggcctccggc agcgcgtacg ccgccgccag
      601 cacctgctga atgtttgccc cctgcggaaa ctgctccgca atcagcttcg ccgctttctg
      661 ctcgctgatg cggtagtggt ccgccacgtc gccggtctcg cccaggttaa gcccggcaat
      721 cagcaggttt ttcagcgcgt actggtcgag atcgcggaac agattcagct gcgccggttt
      781 ccccggccgc agatccagca gggtgaagtc cggacagtgc gcccggaaga cgtgcggcgc
      841 ccatgcgtcc cctttggggt cgaagtacac caccgcgtcc ccgaaatcgc gtacgcagcg
      901 aatgagcgcg ttggtggcca tcacgctctt gccgctcccg ggcaggccga ggatctgaat
      961 gttggtcttg cgccaggtat cacgcggaac cagcaccggc ttgccctgct catccttacc
     1021 cagaaacacc gcctgctcct gccgggcctg ctccgaggaa aaatcggcca gcgcattaac
     1081 cggtaccggc tccccgaaat gcaggtggcg cacgtcggtc atctctcctg tccgctggct
     1141 gctgcgccgg ctctcctgat tcagtttcgc gttcaccgcc tcgtcagcag ccgcaaaatg
     1201 accgtcaagc agcaggaacg ccagcagccg cgccaacaca atcccgacag ccagcccgcc
     1261 cgcgcaccag gcaatcccga acaggttagc cggctgcagc aggtaataat ctgcgtttgc
     1321 gtagaggtcc ggacgcagga cggtttctcc ccagctctcg ataaacacgg cgacaaccag
     1381 cccccccaga agggccagac cggccgcgcc ggtcagccag tacgcgtagt tccagacatg
     1441 tcgacgtgcc ctgaccatcc gcccgggcga atccagcgag gccagcaccg aaaatggcgc
     1501 tggccgttta ttatcgcgct tatagcgatc ggcatactcc cgtcgctcct tatgactcag
     1561 ccccacaaaa cgcatccgtc cggcccaaag ttcagccaga ccgaaaaaca ggccgataac
     1621 taggaaagcc ccggccagac cgtaatactc ctggtacagc cagctgaccc acggataaat
     1681 ctgccgggcc agctccggaa tgtgccactt ccaggcaagc gaatccgtat taaacatgtt
     1741 ctctctcctt cagacgatgg tttgccggcg tgatgtgcag cccccgctgc acaagccgcg
     1801 gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac
     1861 cacggaggcc agcctctttc cgccccatgc ggaaaaggcg cagccccgga ggaacaacga
     1921 gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc
     1981 ggcccccgtc agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt
     2041 tgctgcttcg ccaggtgcgt ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct
     2101 gacgggggcc gggtgaattt ctggcgtggc cggcgtccag ccggacggag ccagaaagag
     2161 ggggagagac cgtgcgtcgg tggcgtccag ccagcgtaag caggggatat ccccctgagg
     2221 tcagccacgc cctcgcgggg atgacctgta tttctccgta aggagaaaaa agcaacagag
     2281 ataggttaac gtaaaatgtt cagcgaaaaa ccgatatgat tcacctatcc tggtaattta
     2341 ttatcaggga atataaagac ttaatcaggg gacaatatgc cacatcagcg cgtccggtgt
     2401 ccgtcttgta ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct acgctccggc
     2461 cgtgagactg cggcgggcgc tgcggacaca tgcaaagtta cccacagatt ccgtgaataa
     2521 gcaggggact aacatgtgag gcaaaacggc aaggccacgc aggtggcgtt tttccatagg
     2581 ctccgccctc ctgactgagt tcacataaac agacgctttt tcggtgcatc tgtgggagcc
     2641 ggtggctcaa ccatgaatca gtcaggagtg gcgaaacccg acaggactta aagatcatca
     2701 ccgtttccga tgaatcgctc cctcttgcgc tctcctgttc caaccctgcc gtttcccgga
     2761 tacctgttcc gcctttctcc cttgcgggaa gtgtggcgct ttctcatagc tcacactctg
     2821 gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aaccccccgt
     2881 tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cggaaaagca
     2941 cgcaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc
     3001 taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt
     3061 tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa
     3121 ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc
     3181 agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgcg atccccgtca
     3241 gttcagagga ggacgatggt gccaccgttc caccctgaac atcccggata tagtttgcct
     3301 gacatgcgac cagaagaaag cctgaaaaaa tcaatataaa aagaaaaaca acctttacct
     3361 ttttcatata ccttccctgt atttaaaaac cccggtattc atccctgaac accggaagtc
     3421 tgtatttcaa atcatccttg ataacttttg cttaccagtt accatcccgg taaacaaagg
     3481 atacaaagta atcaaattta ctaatatcga ttacattttt aaagtgtggc tgtaatatcg
     3541 aaacccatga tttttcaaca tcaaaccaac cattattaat attatccttt aaaggcatcc
     3601 ccagactttc aatgacagaa ccatcatcac cgaagtcttt tgagtattca ccacctttaa
     3661 actcttcggt tttcttatca aaccaatgaa tatgtaattt aagccccata tttacctctt
     3721 aaagatattt tttaatgttt cgttttggat ccggcccttt aacctgctta cccgtttttg
     3781 gatcgaatgc cccgaggtgt tcgccatcac tggcgcggta cccttcaagc tcaccgtgct
     3841 gggaatccca ttcataaatt ttacgctttt tatcaccata ccatcgggcc cgcttaccac
     3901 caccaccttg tttaggggtt ttaggtttac cctctttcaa ctctcccaac cccttaatat
     3961 cttcagtctt gggatcagga aaataatcat ggccgtaatc tttagttccc ttacgaggtt
     4021 ttttcttttc ctcatttaat ttattttctg catccttcgc ttttttctcc ttttgtttcc
     4081 ggctctccat cgccccggaa agcgcagcct tatccctgga taaggattca ttcatttcgt
     4141 ttaaagatgt ctgggcagct tttaatttct cattctgatt ctgaatatcc ttatcaagat
     4201 agcgaacctg atcctgccac ccggagccat attcatgagg cgggttataa gtcacaaaat
     4261 catctttatt tgcctctgcc tcttttacct ttttatcggc ttcctgaaca gcagcccttg
     4321 caccggggat cgccttctct gtcgatgcaa tgcgactgtt taaggtcgca atatttttat
     4381 cttcagcatc cagctcagct ttcgctttat catactctct tttcagcccc tcttccgggt
     4441 gagcagcgtc ccatgcctgc tggcgacgct tttcttcctc cagacgctgt tttacctgtg
     4501 ccggagtagg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4561 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4621 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4681 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4741 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4801 ccacgctcac cggctccaga tttatcaggg cactgttgca aagttagcga tgaggcagcc
     4861 ttttgtctta ttcaaaggcc ttacatttca aaaactctgc ttaccaggcg catttcgccc
     4921 aggggatcac cataataaaa tgctgaggcc tggcctttgc gtagtgcacg catcacctca
     4981 atacctttga tggtggcgta agccgtcttc atggatttaa atcccagcgt ggcgccgatt
     5041 atccgtttca gtttgccatg atcgcattca atcacgttgt tccggtactt aatctgtcgg
     5101 tgttcaacgt cagacgggca ccggccttcg cgtttgagca gagcaagcgc gcgaccatag
     5161 gcgggcgctt tatccgtgtt gatgaatcgc gggatctgcc acttcttcac gttgttgagg
     5221 attttaccca gaaaccggta tgcagctttg ctgttacgac gggaggagag ataaaaatcg
     5281 acagtgcggc cccggctgtc gacggcccgg tacagatacg cccagcggcc attgaccttc
     5341 acgtaggttt catccatgtg ccacgggcaa agatcggaag ggttacgcca gtaccagcgc
     5401 agccgttttt ccatttcagg cgcataacgc tgaacccagc ggtaaatcgt ggagtgatcg
     5461 acattcactc cgcgttcagc cagcatctcc tgcagctcac ggtaactgat gccgtatttg
     5521 cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg
     5581 ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca
     5641 acagtgccgt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
     5701 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
     5761 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
     5821 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaacgttt
     5881 gacatgaggg gcggccaagg gcgccagccc ttggacgtcc ccctcgatgg aagggttagg
     5941 caacactgcg tgttcgctcg aatgcctggc gtgtttgaac catgtacacg gctggaccat
     6001 ctggggtggt tacggtacct tgcctctcaa accccgcttt ctcgtagcat cggatcgctc
     6061 gcaagttgct cggcgacggg tccgtttgga tcttggtgac ctcgggatca ttgaacagca
     6121 actcaaccag agctcgaacc agcttggttc ccaagccttt gcccagttgt gatgcattcg
     6181 ccagtaactg gtctattccg cgtactcctg gatcggtttc ttcttcccac catccgtccc
     6241 cgcttccaag agcaacgtac gactgggcat acccaatcgg ctctccattc agcattgcaa
     6301 tgtatggagt gacggactct tgcgctaaaa cgcttggcaa gtactgttcc tgtacgtcag
     6361 caagtgtcgg gcgtgcttct tctccgcccc accactcgac gatatgagat cgatttagcc
     6421 actcatagag catcgcaagg tcatgctcag tcatgaggcg cagtgtgacg gaatcgttgc
     6481 tgttggtcac gatgctgtac tttgtgatgc ctaactttgt ttggaaatgt tgaatactca
     6541 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6601 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcact tttccccgaa
     6661 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6721 gtatcacgag gccctttcgt cttcaagaat tttataaacc gtggagcggg caatactgag
     6781 ctgatgagca atttccgttg caccagtgcc cttctgatga agcgtcagca cgacgttcct
     6841 gtccacggta cgcctgcggc caaatttgat tcctttcagc tttgcttcct gtcggccctc
     6901 attcgtgcgc tctaggatcc tccggcgttc agcctgtgcc acagccgaca ggatggtgac
     6961 caccatttgc cccatatcac cgtcggtact gatcccgtcg tcaataaacc gaaccgctac
     7021 accctgagca tcaaactctt ttatcagttg gatcatgtcg gcggtgtcgc ggccaagacg
     7081 gtcgagcttc ttcaccagaa tgacatcacc ttcctccacc ttcatcctca gcaaatccag
     7141 cccttcccga tctgttgaac tgccggatgc cttgtcggta aagatgcggt tagcttttac
     7201 ccctgcatct ttgagcgctc tgatctgaat atcgagggac tgctggctgg ttgagacccg
     7261 cgcataacca aaaattcgca taaaaatgta ccttaaatcg aatatcagac acgatgtgtc
     7321 tattatgcca aaatgacgat ttaatggaca ctcaaacgaa gccgttttac tatgtctgat
     7381 aatttataat atttcgaacg gttgcagttg tgttaaaaaa gccgtcaggc agggaggccg
     7441 atatgcccgt tgattttttg accactgagc aggttgagag ttatggcagg ttcactggcg
     7501 aacccgatga acttcagctg gcgcgttatt ttcatcttga tgaagcggat aaagaattta
     7561 tcgggaaaag ccggggtgat cacaatcgac ttggtattgc cctgcaaatc gggtgtgtgc
     7621 gttttctggg cacttttctt actgacatga atcatattcc ttccggcgtc cggcatttta
     7681 ccgccagaca gctcgggatt cgtgatatca ccgttcttgc agaatacggt cagagggaaa
     7741 atacccgccg tgagcatgca gcgctgatac gtcagcacta tcagtatcgt gaatttgcct
     7801 ggccctggac atttcgcctt acccgtcttt tatatacccg gagctggata agcaacgaac
     7861 gtcctggcct gcttttcgac ctggcgacag ggtggcttat gcaacatcgt attattctcc
     7921 ccggagccac cacgctgacc cggttgattt cagaggtaag ggaaaaggcg acgttgcgcc
     7981 tgtggaacaa actggcactg ataccgtcag ccgaacagcg ttcacagctg gagatgctgc
     8041 tggggccaac tgattgcagc cgcctgtctt tactggaatc actgaaaaaa ggccctgtga
     8101 ccatcagtgg tccggcgttt aatgaagcaa ttgaacgctg gaaaactctg aacgattttg
     8161 gcctgcatgc tgaaaacctg agtacactcc cggctgtgcg cctgaaaaat ctcgcacgtt
     8221 atgctggtat gacttcggtg ttcaatattg ccaggatgtc accgcagaaa aggatggcgg
     8281 ttctggttgc ctttgtcctt gcatgggaaa cgctggcgct ggatgatgca ctggacgttc
     8341 tggacgccat gctggccgtt atcatccgtg acgccagaaa gattgggcag aaaaaacggc
     8401 tccgctcgct gaaggatctg gataaatctg cattggcgct cgccagcgca tgttcgtact
     8461 tgctgaaaga agaaacaccg gacgaatcga ttcgtgctga ggtgttcagc tacatcccta
     8521 ggcaaaagct ggctgaaatc atcacgcttg tccgtgaaat tgcccggccc tcagacgata
     8581 attttcatga cgaaatggtg gagcagtacg ggcgcgttcg tcgtttcctg ccccatctgc
     8641 tgaataccgt taaattttca tccgcacctg ccggggttac cactctgaat gcctgtgact
     8701 acctcagccg ggagttcagc tcacggcggc agttttttga cgacgcacca acggaaatca
     8761 tcagtcagtc atggaaacgg ctggtgatta acaaggaaaa acatatcacc cgaaggggat
     8821 acacgctctg ctttctcagt aaactgcagg atagtctgag acggagggat gtctacgtta
     8881 ccggcagtaa ccggtgggga gatcctcgtg caagattact acagggtgct gactggcagg
     8941 caaatcggat taaggtttat cgttctttgg ggcacccgac agacccgcag gaagcaataa
     9001 aatctctggg ccatcagctt gatagtcgtt acagacaggt tgctgcacgt cttggcgaaa
     9061 atgaggctgt cgaactcgat gtttctggcc cgaagccccg gttgacaatt tctcccctcg
     9121 ccagtcttga tgagccggac agtctgaaac gactgagcaa aatgatcagt gatctgctcc
     9181 ctccggtgga tttaacggag ttgctgctcg aaattaacgc ccataccgga tttgctgatg
     9241 agtttttcca tgccagtgaa gccagtgcca gagttgatga tctgcccgtc agcatcagcg
     9301 ccgtgctgat ggctgaagcc tgcaatatcg gtctggaacc actgatcaga tcaaatgttc
     9361 ctgcactgac ccgacaccgg ctgaactgga caaaagcgaa ctatctgcgg gctgaaacta
     9421 tcaccagcgc taatgccaga ctggttgatt ttcaggcaac gctgccactg gcacagatat
     9481 ggggtggagg agaagtggca tctgcagatg gaatgcgctt tgttacgcca gtcagaacaa
     9541 tcaatgccgg accgaaccgc aaatactttg gtaataacag agggatcacc tggtacaact
     9601 ttgtgtccga tcagtattcc ggctttcatg gcatcgttat accggggacg ctgagggact
     9661 ctatctttgt gctggaaggc cttctggaac aggagaccgg gctgaatcca accgaaatta
     9721 tgaccgatac ggcaggtgcc agcgatcttg tctttggcct tttctggctg ctgggatacc
     9781 agttttctcc acgcctggct gatgccggtg cttcggtttt ctggcgaatg gaccatgatg
     9841 ccgactatgg cgtgctgaat gatattgcca gagggcaatc agatccccga aaaatagtcc
     9901 ttcagtggga cgaaatgatc cggaccgcag gctccctgaa gctgggcaaa gtacaggcct
     9961 cagtgctggt ccgttcattg ctgaaaagtg aacgtccctc cggactgact caggcaatca
    10021 ttgaagtggg gcgcatcaac aaaacgctgt atctgcttaa ttatattgat gatgaagatt
    10081 accgccggcg cattctgacc cagcttaatc ggggagaaag ccgtcatgca gttgccagag
    10141 ccatctgtca cggtcaaaaa ggtgagataa gaaaacgata taccgacggt caggaagatc
    10201 agttgggagc tctggggctg gtcactaacg ccgtcgtgtt atggaacact atttatatgc
    10261 aggcagctct ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa gatatcgcac
    10321 gcctctcccc gctttgccac ggacatatca atatgctcgg ccattattcc ttcacgctgg
    10381 cagaactggt gaccaaaggt catctgagac cattaaaaga ggcgtcagag gcagaaaacg
    10441 ttgcttaacg tgagttttcg ttccactgag cgtcagaccc cttttgatcc caatttttcc
    10501 acccagaaag ctgacacaac acgaatgatg gtgccttttt tccggcagtt ctgggatatg
    10561 ggcgcaaagg ataaagcgac cggaaaatca cggagtgatg tgcagcaacg cattcagcag
    10621 tttcacagtc aggaattttt aaactcactc caaggcacaa cacaatttgc gggtaccgat
    10681 taccgcagca aagacctcac accgaaaaaa tcaaggctac tggctgacac catttcagct
    10741 gtttatctcg atggctatga aggcagacag taagtggatt taccataacg cgtcattacg
    10801 cgcaccgctc agacgggctc agggcgtttc cggcggctac gggataaaac aggcaacaac
    10861 ccccccggaa aactgccgga aacgcgcccg ataaatttta tcctaatgca tgcctatgca
    10921 cgcaactgca tggcatatgt tttgacgtaa aatggatgcc gtaagccctt ttttaccgac
    10981 tttctgcttc atcagcctga ctgacacgac catattcttt cagaccgccc cctgctaaac
    11041 ttccttcata ttgccgcatt aacccgggag gctccgcaat gagcaacact acacactacg
    11101 aaaacgccaa ctttcttcgg gaactggctg aaagcctgcc ccgcatactg ccggaaggtg
    11161 gcccggacaa agctgcgctg ttgcagcgac tggctaacga agaactggcc caggcagagt
    11221 atgaagatca ggttcgcgcc aaagtgaccg ccgcccgtgc tgacacgcgt ccgggtatga
    11281 ccacggaaca actccgccag cgcctgcatg gccgttatca ggaactgcgc gatgccgtat
    11341 gacgtggaat ggaagcaggg agccatcgag gatgtaacga cgctgtttga ctatatcgcg
    11401 gaaaattcat ccctgtggga tgcgcagaac gttaccgacc gggttctcgc tgcggcggac
    11461 aaactggccg acttcccgcg actctatgaa gtggactcac gctatggcga ggacgttcgc
    11521 cggatcagtc tgatgggaca gcatgtcctc tatgatgttg atgaccagac cagaaaagtc
    11581 cgggttctgg ccgtggtcgg ccagcgccag aatccccata ttgttctcta ataaccggtg
    11641 acggggacgc aattcattgc gtggccgtcg ccggttggac aagcacagcg cgtcagcgtt
    11701 ttccccacgg ccaccaccgg cgcggtgttc cggccggggt ctctgctgtc tccggtttgt
    11761 gttccagcag tctcatggac tgcatttcct gccgtagccc ggccacctcc tccctgagtg
    11821 cttttatctc cgcgatcaac gcgtcggagg ccattttttc agtctcaccc gacagtctca
    11881 actcgtcccg ctcgtgagtc tcatcctgtc ttaactcacc gtacacccgg ataagctcgc
    11941 tggtatccac cacccggccg ccatcagctt ccgtacggta tgatacgcgg cctttcgcca
    12001 tatcccgata cagggaactt cgggctttac ccgtcagttc cgtcgcctgt ctcaccgtca
    12061 cccacgccat tgtctcaccc cgctattttt gagacagtat acctgacagg cgcacaccat
    12121 gcccggaagg aaagaatcat gacaaaacag gaaaaaacag ccctcaatat ggctaaattt
    12181 attaaggagc agtcgctgct attgctcgat aaactgaatg agctggatat ggataccccg
    12241 gccgacatgt gcgagcggct acacgaggat gcggaaacac tctggcaggt gatgcgggat
    12301 aagctcgggg aggaataaca gcaccggcat ggccggtgct ggcatttatt ttttgccgaa
    12361 cggccaccac cggcgggcag gtttgtcctc cggcagttca ggtaccggca cgttaaaata
    12421 gcggctgtta tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc
    12481 gtgccgctca ctcaccacgt caatcatcgc gttaatctgc tccggtgagc acttcccggc
    12541 atcaaccacc tgcacaccgt tctcgatggc gaaccgctcc accttgcgat agaggtaatc
    12601 acacagcttc attcctgtgg gtttgttccg ttcccattgc ggatttttac cagtgaaaaa
    12661 gtacgtggct ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat
    12721 cgccagatgc tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag
    12781 caccaccata tcttccagat gctcgctggt cggcatgatg cagcgctcgc cgttccggtc
    12841 ctccgcgtct tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa
    12901 tttaaagcac gcaaacaacc gttcacgatc cagctctgcc gttttgcggg cctcgccggt
    12961 ctgtgggttc acgatttcat ccaccgacag gattgcccgc tgcaccctga cctgcgtcgc
    13021 ccggtccggg cagatgtacc agatttcatc gatgccgtta gctttgcggt tgaacagata
    13081 cccggacacc acttcctgat accggcgacg gctcttgacg gtgcgctcca cctcaaacgc
    13141 caccgtttta ccgtccagct ccgccagggc gtccggcact ttcatgcctt taagactcag
    13201 acggtgcaga atgcgccagc cggtggcccc tctggcttcc atatcaaggc gcagggattg
    13261 cacggccagc cggtgcggca gatgtgctgc cgacaccctt gacggctgaa acggaataac
    13321 atccgccagc ggttcgtcct cgtcgaacga gaacgccacg ccatgcggcg tcagccccca
    13381 cacgctgacc tgacggccgt ccaccggata tttggtccgg accagcagcc ctttcgccac
    13441 caggctgtcg aggaagcggt agaccggctg caccgccgca atccccagcc gccgcgcaac
    13501 cagctccgcc gtggaacaca gctcttcccg caaccagcgc agggtcagcc gggttttctc
    13561 cgcgttccgc gcctgtcgtt tcgcgtggct gacgttgtac agctccagcg ccatcagtaa
    13621 atccccgcaa aatcatcatc cgctttcggc ccgccgccgt cggccggggg ccggaactcc
    13681 gccaggggat cggttttcgc cagcaccggg aaacgctgca acgggatttc ccggcgctca
    13741 acccgcagcg gactgctgaa cgccatccgg gccacaccca gcccggtcat gaccgcgaac
    13801 ccgtccggca ggtgttgcag ggtgttcacg tcaaacagcg g
//
