LOCUS       CP055735                2275 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHB13-C14 plasmid pRHB13-C14_12, complete
            sequence.
ACCESSION   CP055735
VERSION     CP055735.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148098
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T1-C14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.3972219793898x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 17:04:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,159
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,830
            CDSs (with protein)               :: 4,830
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 208
            CDSs (without protein)            :: 208
            Pseudo Genes (ambiguous residues) :: 0 of 208
            Pseudo Genes (frameshifted)       :: 85 of 208
            Pseudo Genes (incomplete)         :: 110 of 208
            Pseudo Genes (internal stop)      :: 38 of 208
            Pseudo Genes (multiple problems)  :: 24 of 208
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-C14"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB13-C14_12"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(148..1158)
                     /locus_tag="HVW81_25795"
     CDS             complement(148..1158)
                     /locus_tag="HVW81_25795"
                     /inference="COORDINATES: protein motif:HMM:NF013603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QLM74979.1"
                     /translation="MKNPDSPILSLRDYSTQDSKWDSDRARADQVAKIYASDQAFFRRG
                     ERMFDCSQRLQFAPQSSRLTGEMRLALRHGEFCHVPFCPVCSRRRSLRWMRRLWEALPK
                     LLAEHPTASWLFLTLTVKNPPVGELRETLRQMNAAWERLIKRKAFAPVLGWLRSTEITY
                     GNVRGCCHPHFHVVLWVPSSWFTRDYVKHERWVEIWSECLRVDYAAGAHIKRVRPKRGK
                     VPEGASFAEVQRAALEAGVIETLKYTVKSSEIVRDPAWFLELARQTYGLRMIATGGRFR
                     EILQVEKPETDEDLIGADMPAKPDEFEEMAFWLAFDWRRDEKRYKRNPKADRRKE"
                     /name="protein rep"
     Typing          complement(1740..1822)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.882"
                     /coverage="93.0"
                     /evalue="1.53e-15"
ORIGIN
        1 cagaaacgcg ctcaaaacgc ttctgagcgc cttaaaacga aaaaacgggc aaatacttgc
       61 ccgtttctga aaatcgcgtt agaacgcttc tgagcgcgtt tcacctccac taccgcacta
      121 ttcccacaat gtgcctctga aacacgctca ctccttccgc ctgtctgctt ttggattccg
      181 cttgtagcgt ttctcgtcac gtctccagtc aaacgccagc cagaacgcca tctcctcgaa
      241 ctcgtccggc ttcgctggca tatcagcacc aatcaaatcc tcgtccgttt ccggcttctc
      301 gacctggagg atctcccgaa accgtcctcc cgtggcaatc atccgcagcc cgtaggtctg
      361 cctcgccagc tccagaaacc acgccggatc gcgcacgatt tccgatgatt tcaccgtgta
      421 tttcagcgtc tcgatcacgc ccgcttccag cgctgcccgc tgcacctcag caaaacttgc
      481 cccttccggc actttccccc gttttggccg cacgcgcttg atgtgcgcgc cagctgcgta
      541 atccacccgc aggcattcac tccagatttc cacccagcgc tcatgtttca cgtaatcccg
      601 cgtgaaccat gacgaaggaa cccagagcac cacatggaag tgcgggtgac aacatccgcg
      661 cacattcccg taggtaattt ccgtactccg cagccatccc agaacaggcg caaacgcttt
      721 gcgcttgatt aaacgctccc aagccgcatt catctgccgg agtgtctcgc gcaactcacc
      781 aacgggagga ttttttacag tcagggtcag gaataaccaa ctcgcagtag ggtgctcagc
      841 cagtagtttc ggtagcgcct cccacagacg acgcatccag cgtaacgagc ggcgacggct
      901 acacaccgga caaaacggga catgacagaa ttcaccatga cgcagtgcaa gtcgcatctc
      961 gcctgtaaga cgggaactct gtggagcgaa ctgtaaacgc tgactgcaat caaacatcct
     1021 ctcgccgcga cggaaaaatg cctggtctga cgcgtaaatc tttgccacct ggtcagccct
     1081 cgcgcgatcg ctgtcccatt ttgaatcctg cgtgctgtag tcacgcagtg agaggatcgg
     1141 actatctgga tttttcatga cctgccctca gtaggcaggg gcagaaatct tgtgttagca
     1201 tgttcttacc acctgtgctt ggcttaccga attttacctt tcaggtggtc gcccccggag
     1261 tggtagcaca ctgccgggga acctcaccaa cctgttgctc acactgtagc caaaaaaagt
     1321 tgtaaaaaca atttaacaac catctacaac taaaaaaccc ctcaaacccg cgccattact
     1381 gggctgaaag gcaaaaatgc atccgcgagt tgttgcctat agtataaata caaggcgcac
     1441 cccaaaatcg ctcaatattt gcgcagattt tcagcaccgc aaaaccccgt ttttcgcgcc
     1501 tgttacaccc accccatcca caaccttttc acccgaatct gtgaacaaca aaaaaccgtc
     1561 cggcaggtgg ttggcctcac tgcgtgacac caaccaccac tcccagtgtt tttatccgcc
     1621 atgtcgtcaa tcacaaaatc ggaacccacg ccggaggcgc gggaccccgc gattttctgt
     1681 ttgacgagtc agggaacggc gaacgctgac agatttcaat ctggctgtgt gagacgttgg
     1741 cggggtgtcg gggcgcagcc ctgaccagat ggtgtttgtt tgatggcgcg tgcgcggtcc
     1801 gacaaacaca catcctgtcc cgattttttc gcctgaacgc tggcagatgg gcagatgagg
     1861 ctcggcattt tcgacgaccg gacaacaaat cattttgccg tttctttgag cgtcgcagac
     1921 cgcacacaat cggaacggag tggagattgt ataagtgcgc atttcatgat ttttttccgc
     1981 gcagcaatct ggcgctcgtg aggtccaact cgttcgcaaa aaccgcaaaa tgatttgccg
     2041 tcacatttcc gaaatgttcc gttaccgcgc cggagaaaat ccaaaaaacc ggaagcaaaa
     2101 acgggacagg atgtgtgttt gtcggaccgc gcacgcgcca tcaaacaaac accatctggt
     2161 cagggctgcg ccccgacacc ctagacaccg tgccagcgac acgctaaaac gacaaacgcg
     2221 cgtttctggc accccaggaa accggaagtc cagagagagt tacagtcgac aacgc
//
