LOCUS       CP055733                3136 bp    DNA     linear   BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHB13-C14 plasmid unnamed.
ACCESSION   CP055733
VERSION     CP055733.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148098
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T1-C14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.3972219793898x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 17:04:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,159
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,830
            CDSs (with protein)               :: 4,830
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 208
            CDSs (without protein)            :: 208
            Pseudo Genes (ambiguous residues) :: 0 of 208
            Pseudo Genes (frameshifted)       :: 85 of 208
            Pseudo Genes (incomplete)         :: 110 of 208
            Pseudo Genes (internal stop)      :: 38 of 208
            Pseudo Genes (multiple problems)  :: 24 of 208
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-C14"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(53..265)
                     /locus_tag="HVW81_25765"
     CDS             complement(53..265)
                     /locus_tag="HVW81_25765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLM74974.1"
                     /translation="MRKFAILTRSTAGASETNRVPYLCIAMQHPENAFHAFLGIQPGEN
                     MVFLLFERKDVKNLVIALAIFRRRA"
                     /name="hypothetical protein"
     gene            complement(332..862)
                     /locus_tag="HVW81_25770"
     CDS             complement(332..862)
                     /locus_tag="HVW81_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLM74975.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     gene            complement(865..1791)
                     /locus_tag="HVW81_25775"
     CDS             complement(865..1791)
                     /locus_tag="HVW81_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLM74976.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1967..2362
                     /locus_tag="HVW81_25780"
     CDS             1967..2362
                     /locus_tag="HVW81_25780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLM74977.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     Typing          complement(869..1791)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaacctca atgaacccga agaagctcgc ctaatggcgg gcttttttcg ttctacgccc
       61 gccgacgaaa gatcgcaagt gcaataacga gatttttcac gtctttacgc tcaaaaagta
      121 aaaaaaccat gttttcccct ggctgaatcc ccaaaaacgc gtgaaatgcg ttttcaggat
      181 gttgcatagc tatgcacaaa tacggcacac gatttgtttc gctcgcccca gccgtcgaac
      241 gggtcaaaat ggcaaacttt ctcatcagaa aacggtgaac gggacgcgga attttttcgc
      301 gtcccgtgag gcgttggaca agcgaagcgc gtcagttttt ccggctcttc cctgttgctg
      361 gtttgtccag tttcttttca atatcaacaa ttttctgata cacgacctcc gtcgcggtgt
      421 agttcctgtc gatcaacgtt ttggtaaacc agccaagcaa cgagaacatg accagaaaac
      481 tgaccgtaac accgataaac catttcagac tgtatttatt gagcgtgttg tattctttaa
      541 caacattgtg atgaattgtc gcaatgtcct tgttcagggc atccgcgttt gccttcatgt
      601 ttgcgactga ctcttccagc cgctgaaaat cctggctgat gcgctcacct atcagccctg
      661 caacatccag cttctgcacc tcgtttctga cttcatccct gacctgtttt gccacaccgt
      721 cagaaatatc ccgatatccg cgaagtgttt cccttgccac agccagagaa tcagcaacag
      781 ctttctgttg ctccattgcg gtatgttgca gatgctccac cgataacagg agcgtgagta
      841 atttttcttc tgtgctggcc atgttcagaa tcccatagaa aatccacgtg acggtgattt
      901 aacccgttca ggttcagttc ggttctgtct gctcactctg tctctcatgt caaagccatg
      961 ctcccgcgca acatcatgtt gcagccttct gaccagttca tgttgcttaa ccggagtgtc
     1021 cagccattcc ctgatgtcat gcccgaccgt tcgcgcctgt tcaagatggc gggaatagtc
     1081 cggttcagac cgtttttgca tgatgtcatg acgctgggca tcatcaagcc gtccggcagc
     1141 ttcaaaaccc agatgcactt cgggacgtcg ttcaatgccc tgttctttca gcgtccggca
     1201 gtcaatgaag cccctttcaa agccatgctt tctcatatgt tcgttgtgct tcacctccca
     1261 gcgttttcgc aattcccgca actgctcttt ttgctgagtg agagtcaggc ttccggaatc
     1321 ctttttcgca cctccccgct cgggatacct ggcgttatag cgccggaaat actgttccgg
     1381 tgtacgttca atgccatcat tcacacgctg gctcatcatg atatgcgcat gaggctgttc
     1441 gccgccgtca atgctcgctt tcggattgtg aatggcaaaa ctccaggcgt gccgttcgcc
     1501 agcttcctga cgtacaaaat cctgcaccag ttcaaggcgc tgttccgctg tcagttcacg
     1561 gggtaatgca atttcaagtt cccgataggt cgaaccgttc gcacgttcaa actggtcagc
     1621 cgcctgccag aaatgtgagg gattatctcg cgcccattcc ggcatgtttc cgtgtgcgga
     1681 atattccaaa tcctgacggt cgcggtattt ctctttgcgc tcgatgtaat cggcgtggtt
     1741 tgcagcctgc cccttgccac caaatttaac gctgaggtga taggttgcca cggtttcagc
     1801 cctccgcagg agcgcactgt ttctcgacga aggagagaaa catataagtg cgcttccttc
     1861 taattttacg aattagcacg gaagataccc cctgcaccca ccgaccgcaa gcgatatact
     1921 gcacaacccc aacacaccgc aaaaaacacc agggagcatc ccaaacatgc gcatcagcaa
     1981 catcgactgg ttgaagaaac gaatcggctt tatcaggaag ctcggagaac agaccgcacg
     2041 acagaggcag atcattgacc tgctcgataa cgaagccgga ctaacagaac aggagcgcaa
     2101 actactccac gtcctggcaa ccgcagaaaa aaacgagcta caggcacagg agaacgcacg
     2161 caaacaggcc aaccaaaaac ggatggaggg gaaaacacaa cgacgggaac gcaatcaccg
     2221 cctgtttctt gcggcggggt tactgatcga ggccggacta gtggacacaa aaacaggcga
     2281 actgagatac ccaaaagaca atatattaca gaaacttaag gcaatcaggc tcgatcttga
     2341 aacaagccca gatcatcatt aattccgctg cgtggtggtg cgtttgcctg ccggtgttca
     2401 atgttttcga tttcgcgacg cgctacgcgc ggatctggca caggcgcgta agtgcacaat
     2461 gcttgcctct gtgactcttc cgcttcctcg cccactcgct cactacgttc ggtcgctggg
     2521 gctgcggcga gcggtgggat cactctcgtt gtgatgcaga tccgtttttt tttttgcact
     2581 ggagttatca ggagtagcgg tgcacatggg atcgtgcgat atgctggatg gtggaaggtc
     2641 ggagggaacg gcaacaactc acccctccca gtgaagggac gggtagtttg ccgctctcga
     2701 tatgccggac ggagccaagg ccatcgatca cggggcgcgt cagtggaaga acccgcgcct
     2761 gcgtgacgta tgcgtcttcg ccgcaccatc tcacccgtca cgccaccaca taccgggaac
     2821 tgccgccaag gtctaactca accaatctag ccgcgctaca tacggttttt gtggcaactc
     2881 atcaggtgtc cgctgtcgat gaaactctct gcgccggtag cggaactctc acgcgtgcgg
     2941 tcgccgtcgt cacagctccc ccaaccgcaa acgattctcg caaatacgca agtatctgca
     3001 aaaacaggtt tttttcgggg ttcctgggag actgtgctgt tgaaaaattc ttgaagaata
     3061 cagaaggctc atgcaaactg aatgaactgg tttctgtcgc tcctaacaac agactccaca
     3121 ggaacattga ggaggt
//
