LOCUS       CP055731                3605 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHB13-C14 plasmid pRHB13-C14_8, complete
            sequence.
ACCESSION   CP055731
VERSION     CP055731.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148098
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T1-C14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.3972219793898x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 17:04:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,159
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,830
            CDSs (with protein)               :: 4,830
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 208
            CDSs (without protein)            :: 208
            Pseudo Genes (ambiguous residues) :: 0 of 208
            Pseudo Genes (frameshifted)       :: 85 of 208
            Pseudo Genes (incomplete)         :: 110 of 208
            Pseudo Genes (internal stop)      :: 38 of 208
            Pseudo Genes (multiple problems)  :: 24 of 208
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-C14"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB13-C14_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            458..1456
                     /locus_tag="HVW81_25730"
     CDS             458..1456
                     /locus_tag="HVW81_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QLM74968.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGER
                     VGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPKIV
                     AEYPGARWLFLTLTVRNCSIDALGETLTAMNAGFLNLKRRKEFRGVLGWVRTTEVTRGK
                     DGSAHPHFHTLLMVSPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRKPKDD
                     ESLPCAVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQ
                     LERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
                     /name="protein rep"
     gene            complement(1439..1876)
                     /locus_tag="HVW81_25735"
     CDS             complement(1439..1876)
                     /locus_tag="HVW81_25735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLM74969.1"
                     /translation="MAVAASARNALKIIRCTANKKLSSTKNHVKTIASTFLSPSITVKH
                     SASVGKLLTSIIQFLIDNNSGDIPSFEGLFLPTHHKQHPYHRQNRAQNVSDGLNQPKHI
                     KPLPDAKNRVRKRLSAFLESKKLSAVDFHCRDPETRSIIAA"
                     /name="hypothetical protein"
     gene            complement(2873..3067)
                     /locus_tag="HVW81_25740"
     CDS             complement(2873..3067)
                     /locus_tag="HVW81_25740"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="QLM74970.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCKKTGRF"
                     /name="ANR family transcriptional regulator"
     Typing          2024..2101
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.25"
                     /coverage="87.0"
                     /evalue="2.63e-28"
     Typing          complement(1176..1365)
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.476"
                     /coverage="99.0"
                     /evalue="1.71e-93"
ORIGIN
        1 agaactgttg cggttattaa atccattggt gggaagactc gcgggaagct ttcgggttat
       61 gcgccttact acggtaatca aagggttgtg gctgagattc atacgccgag tggtgttatt
      121 acggatgttc tttcgtgtgc tgggtgctac cagtgggagc tcgtggattg atgcgttttt
      181 aggtggtcgc caccttgtat atatacttaa gggataaatc gacaactcga aatcatgctc
      241 taggccttgt cacgactggc ctgaacgtgg atttgcgtgg aaataggttt gagagtattt
      301 tgtcggaagt ggtcgcatta aaagcgaccc ccgcaacgat gggcgtcgtt cgggggtgtg
      361 accacctgta ggaattcgaa ttggtgacac gacaacacag gtgatacagg aatgataaca
      421 cgtaatttcg tctgtcgctc actagggaac ggcagacatg aaaaatcctg aacttaaaaa
      481 tctgacggat tattccccgg aggataaacc gtgggatgag cgccggagct tttccgacga
      541 tgtgggcggt atttatctgc gtgctgccgg atttgagcac tacggcgaac gtgtcggggc
      601 ttgtagtggt attttgcggt ttgggtggtc aacaaacaca gagaccggag aaacgcgtct
      661 gcggttgcgt gatgcgcggt tctgtcgtgt ccggcactgt ccgatctgcc agtggaggcg
      721 ctccatgatg tggcaagccc gtttctatca gtccctgcca aagattgtgg cggaatatcc
      781 aggcgcgcgc tggttgtttc tcacgctgac ggtgcgcaac tgttccattg atgcgcttgg
      841 tgagactttg accgcgatga atgcgggttt tttgaatctc aagcgtcgca aggagtttcg
      901 tggtgtgctg ggctgggttc gtaccacgga agtaacgcgc ggcaaggatg gtagcgcaca
      961 tccccatttt cacactctgc tgatggtttc cccctcgatg ttgtccggcc gtgagtatgt
     1021 gaaacatgcc cgttgggtgg aactgtggcg tgattgtctg cgggtggcgt atgacccgaa
     1081 tgtggatatc agggtggtga agtcacggaa accgaaagac gatgaatctt taccttgtgc
     1141 tgttgcggag cagctgcgcg gcgcggtggt tgagactctg aaatattcca caaagcctgc
     1201 tgatatgctg gctgatcctg aatggttcct cgaactaaca cggcaggtac acaagcgtcg
     1261 ttttgttgct acggggggag cgcttaagaa cgttctgcaa ctggagcggg aaacgaacga
     1321 ggatttaatc atggcggatg gcatggcaga gggtacagat gacggttccc gtctggcgtt
     1381 ttcgtgggtg ccggttgtca aaaaatacca gcgcgatcgg gcgaaagata aacgtcggct
     1441 aagcagcgat gattgagcgc gtttctgggt ctctgcaatg gaaatccacg gctgacagct
     1501 ttttgctctc cagaaacgca ctcagacgct ttctaacgcg attttttgca tctggcaatg
     1561 gttttatatg ttttggttga ttcaggccgt cagagacgtt ttgagcgcga ttttgacgat
     1621 gatatggatg ctgtttgtgg tgtgtgggta aaaaaagccc ctcaaaagag gggatatcac
     1681 cggaattatt gtcaatgaga aattgaatta tggaagtgag caacttcccg acagatgcag
     1741 aatgtttgac cgtaattgaa ggagacagga aggtgctcgc gattgtttta acgtgatttt
     1801 ttgtagatga caattttttg ttcgctgtac atctgatgat tttgagcgcg tttctggcgc
     1861 ttgctgctac tgccaaacca tccccatggt cagttgatgc cagaaacgcg caggagtttt
     1921 gtagcgcgtc tccggtccct aatcaatgaa ttcccggtca ttaacatgcg tttacccgga
     1981 aacgcgccct gatgttctat cgtattgcgg gaactgtgtc gagggtgtcg ggcaaagccc
     2041 tgaccaggtg gtaatcgtat atccgtgcgt gcgcggttat acgattacac atcctgtccc
     2101 gaaatcagga ttgtttttca cgtacaactg cggcaaattc accccgttgc ggttgcaacg
     2161 atgggaatat caaaactacg aaaattattt agccatttgc tacccctctg gccctggcct
     2221 ccaccagttc cggggggggg gatttttttg ttgcctctca tgacgaacta cggcaaattt
     2281 ctgacgttgc aaactgcaac acataaaaca cacacaaaac tcacaattgc catgtcctca
     2341 ttgacacccc cttgccatat agggggattt tttcgcctgt cctgattgtc ttttgccgat
     2401 ggttacggca gattggaaaa gagcgcattt gtggtgctct ctccaaaata atatttctca
     2461 ttactcaaac tctgatggct tccccctgta atccaggggg attttttaat ttatcgctat
     2521 gcttcgtaat tcattcagaa tacagacaat atggttaatc agtttttcat catttttgcg
     2581 gtgtttgccc tgtcctgcat cagaacgcta aaaaaacagg aatctcttta tgacaaatcc
     2641 tccatttcag gattttttat gtagcgtcta ctacatgcga tccgttggtg agctgtttat
     2701 tatgcattag ttatataata actacatatt agttatactt ttattgtgta tcagttgcag
     2761 gggcgttttg agggtaaatt ctggaggcac gtgaccgact gtatacaaaa tgctggcgca
     2821 ttttgatacg tcgacgcgcc aaaggggacg cccctttgaa accccgccag aattaaaagc
     2881 ggcctgtctt tttacagtat tcagctctgg ctatacacca acctatattt tctcctttgg
     2941 cataatgaag cgcatcattc cattttttcc atgctccaat gtaatcacct tcacgctcca
     3001 gtctgacagc ttcgtttgcg ggtgaaaaat atttattttc taaataaatt gctttgtcct
     3061 gattcatact cgtaatttat atttttattt gaattaaaaa agagcggcct gtcgggttaa
     3121 tacatattgg catgactgtt gatatatcag cccaactgca cgctcgtaat cgttttaacg
     3181 tgattttcgc aacatggcaa tccgggttca tctgtccggt aatcaataaa aaccgtgcaa
     3241 aaaacgggac aggatgtgta tttgtcggac cgcgcacacg caatcagaca aatgccgcct
     3301 ggtcaggact gcgccccgac accccgccaa cgtctcacgc agccagattg aaatctgtca
     3361 gcgttcgccg ttttctgact cgtcaaacag aaaatcgtgg ggtcccgcgc ctccggcgtg
     3421 ggttccgatt ttgtgattga cgacggtacg agaaaaaata ctggtgatgg tggttggtgt
     3481 cacgcagtga ggccaaccac ctgcaaagcg ggtttttagg aggatgggaa ttgaaaaata
     3541 tctatcagct atacgaggaa aattgcaaca gagtcggatt taaggttcgg cggacatcat
     3601 ggggg
//
