LOCUS       CP055727                6988 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHB13-C14 plasmid pRHB13-C14_4, complete
            sequence.
ACCESSION   CP055727
VERSION     CP055727.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148098
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T1-C14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.3972219793898x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 17:04:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,159
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,830
            CDSs (with protein)               :: 4,830
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 208
            CDSs (without protein)            :: 208
            Pseudo Genes (ambiguous residues) :: 0 of 208
            Pseudo Genes (frameshifted)       :: 85 of 208
            Pseudo Genes (incomplete)         :: 110 of 208
            Pseudo Genes (internal stop)      :: 38 of 208
            Pseudo Genes (multiple problems)  :: 24 of 208
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-C14"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB13-C14_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6863..6988,1..108)
                     /gene="mbeD"
                     /locus_tag="HVW81_25605"
     CDS             join(6863..6988,1..108)
                     /gene="mbeD"
                     /locus_tag="HVW81_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLM74945.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="mbeD"
     gene            complement(592..1014)
                     /gene="exc2"
                     /locus_tag="HVW81_25610"
     CDS             complement(592..1014)
                     /gene="exc2"
                     /locus_tag="HVW81_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QLM74946.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            1136..1414
                     /locus_tag="HVW81_25615"
     CDS             1136..1414
                     /locus_tag="HVW81_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLM74947.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(1406..1696)
                     /locus_tag="HVW81_25620"
     CDS             complement(1406..1696)
                     /locus_tag="HVW81_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLM74948.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            1936..3582
                     /gene="cka"
                     /locus_tag="HVW81_25625"
     CDS             1936..3582
                     /gene="cka"
                     /locus_tag="HVW81_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="QLM74949.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(3579..3869)
                     /gene="cki"
                     /locus_tag="HVW81_25630"
     CDS             complement(3579..3869)
                     /gene="cki"
                     /locus_tag="HVW81_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="QLM74950.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            3899..4030
                     /locus_tag="HVW81_25635"
     CDS             3899..4030
                     /locus_tag="HVW81_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QLM74951.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5036..5227)
                     /locus_tag="HVW81_25640"
     CDS             complement(5036..5227)
                     /locus_tag="HVW81_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLM74952.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5295..5618
                     /locus_tag="HVW81_25645"
     CDS             5295..5618
                     /locus_tag="HVW81_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLM74953.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5581..6324
                     /locus_tag="HVW81_25650"
                     /pseudo=""
     CDS             5581..6324
                     /locus_tag="HVW81_25650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6296..6856
                     /locus_tag="HVW81_25655"
     CDS             6296..6856
                     /locus_tag="HVW81_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLM74954.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          4864..4951
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(4297..4853)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5609..6987
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.783"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gagaacgggc aactgaggga acagtgcgac gacttgagtc agcaggtgca gagattagcc
       61 gggcaggtgg accacttgag tcggttattt atcacgaaca gcagataagg gatgaacagg
      121 aaaggcaggc acgcctggaa cgggagcgtg cgcggtcctg gcatgaagag caggaacggg
      181 aagcacgggt atggcgtggc ccgtcaatag ggatgtgacg gtgagttgtt ctgggaggtt
      241 gccagatggt accggaacgc gttaaaaacg catccgggcg cgattctggc gtgtcgtttt
      301 tgttgctgtg ttttgcctct ttgccagatc gcgctcattc ctcagcctca tcccgcgatg
      361 tctgcgtatg tcatcaactg aaaagatgga aaaaaaacag tgtcatgagg tttacgcagg
      421 caaaactagc gttattcaca tggctgaata gctatgcata cggttaaaat ttatcaggtg
      481 cgatcgcggc agcttttcgg gtggtttgtt gccattttta cctgtcagca gccgtgatcg
      541 cgctgaacgc atttcagcgg tgcgtacaat taagggatta tggtaaatcc cttactgtct
      601 gccctcgtat ccatcgagat aaaccgcaga aatggtgtct gccattagtt tcattttttt
      661 cggcgaaggc atatcgctgt ttgtgtattt tctgccagca aaggctgttg tgctccggag
      721 tgagtgaaaa aatttttctg agtggaattc ttttacacgt tgctgtgccg tttctgctga
      781 tactccggct gctttgtctt tcatccccat atcccggaac tgctgaaaga acgggagcat
      841 catacgtatg gtgtctgctt tctgggtgta gaagttagga tcaaatccat catctgaagc
      901 ataaacataa tgccgggtat gtcgttccag gctggtcttt ggtgtgcatc ctgtaagagc
      961 tattgttgct gttatcagag gtatcagaag cagcctggca gcggcagatc tcataaacat
     1021 ctccttgcgt ttttaatctt gatactcagg gagttatatc tccgaagagt atctcttatc
     1081 atacgtcggt ttactcccaa tgtgatttga tggtcaatga gtcaggaata atcccgtgaa
     1141 aaaatgtgtg ccagtcctgc tttttagtgt tctttctgct tcggttgcca gtcatgcgga
     1201 cagcaatgtt tttcaacaaa aggccgagaa gacaaaaaac ggcattgttg cggatgttaa
     1261 tgccagacag aaaatgttca gtaacagact gaagatacaa cttcagatcg atgagttgag
     1321 agcagagctg gctggcacca gagatgccca aaaaagagag gatttgcgta aaaaactgga
     1381 gtctctggag cggcaaaaga aatccttacg ctgatggggg cgtattttcc ccggtttctt
     1441 ccataacctt tttaccgttc agatatttac gggcgaggcg aatgcgtctt gcctggtata
     1501 ccgccgagta agcctgggtg ggattcacac cattactgat ttcactgacc acatgacgct
     1561 gatcagccag tgtcagcgtg taaacctgtt tctggatgag gtcacgggct ttgtcatgct
     1621 catatccggc agcaacaagg gtgtcgagaa gtcgtttgtc tgttttgttc agtttatggc
     1681 ggggtgattt gctcacatcg ttctccgttt tttcatgatc caacagtttt atatctcatc
     1741 ctgatttgct ccggtgttct gtttcgacgt tctcaatgcc tgcctcccca tagtgtgatc
     1801 cttattgcaa aaatgaaaat ccatgctctt gacatggaca atgctgagta gtaggtttta
     1861 ctgtatataa aaccagtggt tatatataca gtatattgtt tttaatttat tgttttaatt
     1921 atcaaagagg aatttatggc taaagaacta agtggatatg gaccaactgc tggtgagtct
     1981 atggggggaa caggggcgaa tctgaatcag caaggtggaa ataataatag taatagtggg
     2041 gttcactggg gtggtggctc tgggcatggt aataatggtg gtcagggaaa ttcaaattca
     2101 tctggttcta catcaactgt gatgaaaacc ggtgagtcat atctgactcc gtggggtgat
     2161 gttgtcatta ataatgatgg ccttccggtg atgaatggta ttgttatgac agaagagaat
     2221 tcaactctgg ttgataatcc atttggaggg gtctccagag ttcttaattc tttaattagt
     2281 gatatgccat cactttttgc agaaagctca ggaaataaca acaataacac tgcttcagtt
     2341 aatactgcac caacgaatgc tcaggtcagt gatatggata agagttcgaa ggtggtgggt
     2401 aatgtaatta atgaaaaaca aaagcagaag aataaaattg ctacacagat tagtgaaaaa
     2461 cagaagaaaa tcgaggagat gaaaaaagta ttcaaacatc attcttatca tggtatcact
     2521 gatcttgaga gggatgttga tgagttgcaa aaaaaatcaa atcagcttga tgcagatatt
     2581 agcaaactta attcttataa aaatacattg caaagtaaaa ttggtgatgt gaataaacag
     2641 aaggaagctg aagaaaaggc tcgtgaaaat gcagaggtcg ctgaacatga aactcttaat
     2701 gaagagaaac aggcggtggc ggaagcagag aagcgtttag ctgaggcgaa agctgaactg
     2761 gcgaaggctg aaagtgatgt acagagtaag caagctactg tttccagagt tgcaggagag
     2821 cttgaaaacg ctcagaagag tgttgacgtg aaggtcaccg gatttccggg atggcgtgac
     2881 gttcagaaaa aactacagcg tcagcttgaa gctaagcagg ctgagtactc cgcagttgaa
     2941 aatgaactta agaatgctgt tagtttcagg gatggtaagg ccgctgaggt taaagaggca
     3001 gagcagaaat taaaagaagc tcaggatgct cttgaaaaga gccagataaa agatgctgtt
     3061 gatactatgg ttggatttta tcaatatata accgaacaat atggagaaaa atacgcaaaa
     3121 attgctcagg atttagcgga aaagtctaaa ggaaagaaaa ttcaaggtgt tgatgaagct
     3181 ctggcagcat ttgaaaagta taagaatgta ctggataaga aattcagtaa ggttgatcgg
     3241 gatgctattt ttaatgcgtt agagtctgtt aattatgatg agttatctaa gaatttaacg
     3301 aagatatcaa agtcgcttaa aatcaccagt cgagtatctt ttctatatga tgttggttct
     3361 gattttaaaa atgcaataga aactggtaac tggcgacctt tgtttgtcac gctggagaaa
     3421 agtgcggtcg atgtaggtgt ggcaaaaatt gtggcattaa tgtttagttt tattgttggt
     3481 gtgcctcttg gtttctgggg gattgcaatt gtcacaggta ttgtttcttc ttacataggg
     3541 gatgatgagt tgagcaaact taatgagttg ttagggattt aatttctctt tagagtgtaa
     3601 tatatgaaac ttggcaatcc tactgggatt gccattaata aataaaacaa tgcagataac
     3661 ttgcataagt accagggttc tttttttaac tttgtatttt ctttgatata tctagatatg
     3721 gttaatttag agtatggata tagcaataca tgtattgatg atataaatat aaataacaaa
     3781 ggagtgttat cattgtcgtt aaatattaaa ataagaatcc atggtataag tgattcaggt
     3841 aaattatgta ggtagtattt taaatgcata agtattttta cttttgttgg aaagaaatat
     3901 gaaaataata atttttctaa ttacaatatt attcttgtca ggatgtcaag caaactatat
     3961 tcgtgatgta caaggaggaa ccgttgcacc atcttcgtct tctgaactga cggggatcgc
     4021 ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4081 tgcctgtaaa cgaaaaaacc gcctggggag gcggtttagt cgaaggttaa gtcagttggg
     4141 gactgcttaa ccgggtaact ggctttagtg gagcacagat accaaatgct gtccttccag
     4201 tgtagccgct ataaggcttc aacttcaaga gttctgcgta tctaaatcca ctatccagtt
     4261 accaatggct gctgccagtg gcgttaagtc gtgtcttacc gggttggact caagacgata
     4321 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4381 ggagcgaacg acctacaccg aactgagata ccaacagcgt gagctatgag aaagcgccac
     4441 gcttcccaaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4501 gcgcacgagg gagccaccag ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc
     4561 cacctctgac ttgagcgtcg gtttttgtga tgctcgtcag gggggcggag cctatggaaa
     4621 aacgccagct gcgtggcctt ttccgggttc ttgttttttg ctcacatgtt tcttttggtg
     4681 ttatcccctg attctgtgga taaccgcatt accgtctttg agtgagctga taccgctcgc
     4741 cgcagccgaa cgaccgagcg gagcgagtca gtgagcgagg aagcggaaaa gcgcctggac
     4801 gagcattttc tccttacgca tctgtgcggc atttcacacc cggcatggtg cacttttcat
     4861 acaatcctca ttgatgccaa atgattaagc cagtatacac tccgctatcg ctacgtgact
     4921 gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga cgggcttgtc
     4981 tgctcccggc atccgctcac aggccagctg tgaccgtctc cgggagctgc atgtgtcaga
     5041 ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg tcagcgtggt
     5101 cgtgaagcgc ttcgcagatg tcagcctggt cgtcggcgtc cagttcattc agtttttcca
     5161 gcagggtcag cgtctggctt ctgatgaagc gggccatgtt aagggcggtt ttttcctgtt
     5221 tagtcacagt tctgtttctc catgtgtggg gagtctgtcg gaggttttta atgataccgg
     5281 tgaaacgcga aaggatgctc acgatacggg tcacggatga ggaacatgcg cggctgctgg
     5341 cacgttgtga gggaaaacaa cttgcgtcat ggatgcgtaa ggtctgcctg ggggcaccgc
     5401 catcgaaaac gtcaggtctt ccgacactgg caccgccgct gttgcgccag ttcgcttcgg
     5461 tcggcaataa cctgaatcag atagcccgga agataaacag cggacagtgg tcggggcatg
     5521 accgggttca tgtggtggct gcactgatgg ccatcggacg tgaactgagt gagctgaggg
     5581 atgaggtccg taaacaggga gaacgggatg atagttaagt ttcatgccag gggaaaaggc
     5641 ggtggcagtg gtccggttga ttacctgctg ggccgggagc gtaaccggga aggggcgaga
     5701 gttctgcggg gtgctcccga agaggtgcgg gaactcatcg atgccacgcc gtttgcgaaa
     5761 aaatacacct cgggggttct gtcttttgca gagcagaccc tgccaccggg agagcgtgaa
     5821 agggtgatgg agagctttga gcgggtactg atgcccggtc ttgaaaaaaa tcagtacagc
     5881 atcctgtggg tggagcacca ggacaagggg cgtcttgagc tgaattttgt catcccgaat
     5941 atggagctgg cgagcggaaa acggctccag ccctattacg accgagcaga ccgtccgcga
     6001 atcaatgcct ggcagacact ggtgaatcac cattacgggc tgcacgaccc gaacgcaccg
     6061 gagaacaggc ggacacttgt caccccgaat aatctgccga aagcgaaaca ggaggccgca
     6121 gaagcgatta cgaggggact ggaggctctt taccatgccg gagcgctaaa aacgcgtcag
     6181 gatgtcactg aggcgcttac agcggcaggt tttgaggttg tgaggacaac caggagcagt
     6241 atcagtatcg ccgacccgga gggtgggcgt aatctcaggc tgaaaggggc gctgtatgag
     6301 caatctttta caaatgggga cagacttcga gaagaaactg aaagagcgtg ccgtctctac
     6361 agagaacatg ctgaacagcg agttcaggaa gctcgaagcg tctgtagaca gggaactgaa
     6421 cttaaacggg cagaaaatca gagacgctat cagcgcacac acagcagcgg tgaaagaaca
     6481 actggagacg ctgaacagtg ccgtgaacgc gcagttcagc gccacagaga acgaactggc
     6541 ccggcggcag gaagagctgc tctggaagct ggtcaaaggg cggatactgt acccgtcgct
     6601 gacggcgctt tgcgtcacgg gcgggatttt tctggcgtca tgggggctga ttcagtggca
     6661 ggaaagccgg attgcggcga acatcctggc tatcagggac caggaagaga cgctggcgaa
     6721 actccgggaa aaaacatggg gtgtgacgta tcacgagggc aggaacggga aatttctggt
     6781 gttgccgtcc ggggtgaagg gggaaaacaa ctggacggtg gagaagaaga acgcagtaag
     6841 actggtcagg gagtaagtga gtatgacaga gctggaaatg cacttactga acgccttaga
     6901 gcaattgcag caggactata cgcagcggct gaaagactgg gagagcgcct tcacggattt
     6961 gcagcatatg ttcgagctga cacgacag
//
