LOCUS       CP055463                2507 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00489 plasmid pRHBSTW-00489_6,
            complete sequence.
ACCESSION   CP055463
VERSION     CP055463.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148633
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00489
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.108404575331x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 17:26:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,652
            Genes (coding)                    :: 4,435
            CDSs (with protein)               :: 4,435
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 90 of 217
            Pseudo Genes (incomplete)         :: 115 of 217
            Pseudo Genes (internal stop)      :: 31 of 217
            Pseudo Genes (multiple problems)  :: 17 of 217
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00489"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00489_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            470..673
                     /locus_tag="HV216_23805"
     CDS             470..673
                     /locus_tag="HV216_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLO50010.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGGDAGAMMLDAYRQILSET
                     EPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(964..1149)
                     /locus_tag="HV216_23810"
     CDS             complement(964..1149)
                     /locus_tag="HV216_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLO50011.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1515..1742)
                     /locus_tag="HV216_23815"
     CDS             complement(1515..1742)
                     /locus_tag="HV216_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QLO50012.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          830..917
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..191)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.021"
                     /coverage="99.0"
                     /evalue="2.5300000000000007e-56"
     Typing          complement(1978..2484)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.774"
                     /coverage="99.0"
                     /evalue="6.33e-132"
ORIGIN
        1 atgcttgtca ggggggcgga gcctatggaa aaacggcttt ggcgcggcct tatgctttct
       61 tcgttaagta tcttcctggc atcccccagg aaatttctga tccatccgta agcccgtccc
      121 gctcgccgca gccgaacgac cgagcggagc gagtcagtga gcgaggaagc ggaatatatt
      181 ctgtatcaca ttttctcctg acgcgttttc tttcactttc tgcgcctgtc ttatgtggca
      241 ttaatgctat gtgttactgc catgctacat cttaagccag tatacactcc gctagtgctc
      301 cgtgactggt ccggcgctgc gcccggaacc cgcctgtacc ggttcagcag ccgttccggc
      361 ctgactgcaa tttttttttt ttcatccctg cccgctaccc tgtaaacctt tcttctgcgt
      421 tgccgttaac agtaggtaag ccatgtttgg tttttcttcc ggagccctga tgactcttag
      481 tgatgatgag cgacacctgc ttgtgtccgt tgtttcggtc tggctacggc gtgccggagg
      541 ggatgccgga ggggatgccg gagccatgat gctggatgca taccggcaga ttttgtctga
      601 aaccgaaccg gccgtaagga ctgtcatgct cgaatttctt gaatccgttc gcattcatta
      661 catttcgtcc tgagcatttt ctgtatttct gtccatactt aaacgaggcg gccgtcacgg
      721 acggtggtcg tcgttccatg atgctgttat ggacgagtga gagtaatcta atctaacttg
      781 attgagttcc gggcgtgccg cccggttttt tttgctgttt cccggtgtcc gttccgggac
      841 aggatgtgca tttgtaatac cgcgcacgcg ccggcatcac aaataccctg gtcagggctg
      901 cgccccgaca ccccgccatc gcctcacggc cgcgcactga attgcgcacc cgttcaccga
      961 ttttcacgca ttttcctcgc ctatctgtgc gttcagcatg gcgtaaagcg cttcggcctg
     1021 atcgtgcagg tgctcacagc agccggcagc ctcatccaga tccatctgct cgagccgctc
     1081 aaggaggaga agtgtctgtg cgcggataaa tttggccata ttgagggcgg cggattcctg
     1141 tctggtcact ggtgattttc cccgtctggt gatgattttt gcgtgaaaag agcatggcgt
     1201 cattctggtg catagtcatg catgccgtta aaatttaaca ggcgcgtttc tgacgggttc
     1261 cggggtggtt tgttgtggtt tttgtcatgg tttcgtcagg aacgcgctga acgcgtctca
     1321 ggcggtgcgg gcaacggatg ttatggtaaa ttcttggggg cttgattata aaataccgca
     1381 cttcctctta tgtcttgtgc tttttagaca accctgaaag cttttagctt tggtgatgca
     1441 atttgctggg attttggcgg agaaccacag gtaaagaaaa aggccacatt agcggccttt
     1501 ttcggagaag atgctcagtg cggaatttcg gagacttctt taacgtcggt tttattgatc
     1561 tgctgtttga caccattcgc gtctgtatag ccaagcaggc ctgagtctga ttctttaggt
     1621 ttgccgtcgc tgacaatagt gcgaccgtca ttggtgtgta tggcatagct tgtgcgcgtg
     1681 cagccggtga gggctgaaag cgcaactgcc gctgcgaaca ctgaaacaaa aatctttttc
     1741 aaagccagct ccttttatcc atatcagttt tggtcactaa ccatctaact ataggtcatt
     1801 ttttatgtga aaagaggctt gatggtgggc tgtatatcga gttaggcgcg aggtgctatg
     1861 gtcaaagtgt ggtgtcaggc ggggaggagg cttatatgag atgtaatccg gccgatacat
     1921 ataccaagat gatttaagta ttcctctttg tgaggttgga tgtctaattt ataaaggatc
     1981 ttcttgagat cctttttttc cgcgcgtaat ctcttgccct gtaaacgaaa aaaccaccct
     2041 ggcaggtggt ttttcgaagg ttaggtaatc ctggcagatc ccctaaccgt ggtaacagtc
     2101 ttgtgcgaga catgtcacca aatttgtcct ttcagtgtag cctcactaag gccgccactt
     2161 caagaactct tgagacatct ctcgcacatc ctgtttgcca atggccgttg ccaatggcga
     2221 ttagtcgtgt ctttccgggt tggactcaag ttgatagtta ccggataagg cgcagcggtc
     2281 ggactgaacg gggggttcgt gcatacagtc catcctggag cgaactgcct tcccggaact
     2341 gagtgtcagg cgtggaatga gaaaccgcgg ccataacagc ggagtgacac cggtaaaccg
     2401 aaaggcagga atgcggggga gcacgaggga gccaccaggg ggaaacgcct ggtatcttta
     2461 agccgcatcg ggtttcgcca ccactgattt gagcgtcaga ttctgtg
//
