LOCUS       CP055462                2893 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00489 plasmid pRHBSTW-00489_5,
            complete sequence.
ACCESSION   CP055462
VERSION     CP055462.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148633
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00489
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.108404575331x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 17:26:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,652
            Genes (coding)                    :: 4,435
            CDSs (with protein)               :: 4,435
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 90 of 217
            Pseudo Genes (incomplete)         :: 115 of 217
            Pseudo Genes (internal stop)      :: 31 of 217
            Pseudo Genes (multiple problems)  :: 17 of 217
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00489"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00489_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2610..2893,1..451))
                     /locus_tag="HV216_23790"
     CDS             complement(join(2610..2893,1..451))
                     /locus_tag="HV216_23790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="QLO50009.1"
                     /translation="MGNSKRNIKKLNDNFRESILDYAISHNLKCANALIALYATGCRPD
                     EIETGIKVNFDSKNNKLSFKIIGSKLNYQQKRGIRIREVTVKITDKNLQYYKPIIDIFE
                     KNPQAYDLKIKTESAKAFSGYITKISKKLWPRKKYHASAYSFRHAKATELKNSEGFDAE
                     KIAKIMGHASVRSQQSYGRKNKRSAGGFDDITDVETSSKPRGGDRLLRFKIASKQQTAA
                     KIAAISTPPDVASPPPVRRLKR"
                     /name="site-specific integrase"
     gene            complement(444..1148)
                     /locus_tag="HV216_23795"
     CDS             complement(444..1148)
                     /locus_tag="HV216_23795"
                     /inference="COORDINATES: protein motif:HMM:NF013122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="QLO50007.1"
                     /translation="MATKIENDEVIPDEELNKIIEEMNNEEDKRLKVIVDEYQQLTKGG
                     FFVLDTETTDLVGEVVSFSLIDDKNNILINEYCKPTRNKISKTAQSIHNISNKMIKDSR
                     NIIPIYEDFLKIINTEENKNKTIFIYNEGFDLSTIYRSLNYAGYKYKENNDFDDVEFRS
                     RCVMKDFAYYYGEHNEYYDTENYKWKKLEFAANYFGIETKNLKLHNSAVDCYLTLKVIE
                     NIRDHFSGRNYG"
                     /name="3'-5' exonuclease"
     gene            complement(1530..2120)
                     /locus_tag="HV216_23800"
     CDS             complement(1530..2120)
                     /locus_tag="HV216_23800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLO50008.1"
                     /translation="MTKKNNVEILHKEKTNIENDSEIEHKKNPFVFNMSIDTKKKKLTV
                     DRGSEVETEEGVFTTTISQIQEVDREEFLKIFTGQIKFYFDLSQTGFKLFFIIMGLYQK
                     QIGGDEIYLNFETAKEEAEKYESAISKAVYYRGLKELLEKKMIAKSVKRYIFYINPAII
                     FNGDRARFVKEIKIKEEEKQQEENLKILRSKYA"
                     /name="hypothetical protein"
ORIGIN
        1 cttttgcatg tctaaaagaa tacgccgagg cgtgatattt tttcctgggc catagttttt
       61 tgcttatttt tgtaatgtat ccggaaaacg cttttgcact ttcagtttta atttttaaat
      121 catatgcttg agggtttttc tcaaaaatat ctattatagg tttgtaatat tgaagatttt
      181 tatctgtgat ttttaccgta acctctctaa ttctaatacc tcttttttgt tgataattta
      241 attttgagcc gattatttta aaacttaact tattgttttt actatcaaaa ttaactttaa
      301 ttcctgtttc gatctcatca ggtctacacc ccgtcgcata taatgcgatc aaggcgttag
      361 cgcattttag gttatgcgaa atcgcataat ctaaaatact ttctcgaaaa ttatcattta
      421 gtttttttat atttcgtttc gaattaccca taatttctac ctgaaaaatg atctctaatg
      481 ttttcaatta cctttaaagt taaatagcaa tcaacggctg aattatgcaa ttttaagttt
      541 ttcgtttcga taccaaaata atttgcagca aattcgagtt ttttccattt ataattttct
      601 gtatcatagt actcattatg ttctccgtag taatatgcaa aatctttcat tacgcaacga
      661 gatctaaatt ctacatcgtc gaaatcgtta ttttctttat atttataccc tgcataattt
      721 aaagatctat atattgttga aagatcaaag ccttcattat aaataaaaat agttttattt
      781 ttattttctt ctgtgtttat tattttcaaa aaatcctcat atataggaat tatatttcta
      841 ctatctttga tcattttatt agaaatatta tgtattgact gtgctgtttt tgaaatctta
      901 ttcctggttg gtttgcaata ttcattaatt aatatattgt ttttgtcgtc aatcaatgaa
      961 aaactaacaa cctcaccaac cagatctgtt gtttctgtat caagaacgaa aaagcctcct
     1021 ttcgtaagtt gttgatattc atcaacaatt actttcaatc ttttatcttc ttcattgttc
     1081 atttcttcta ttattttatt taattcctca tctggaatta cttcgtcatt ttcaattttt
     1141 gttgccataa tttcacctca tttattgttt tttatattgt taacatcaaa ttccggagat
     1201 ttcaaaaatt cgctttgctc aaatttgaaa taccgtaaaa cccaaaccat ccccccatta
     1261 tttaaatttt tcgcgttgct tcaaaattca aacaatgacc cccggttggg ggtggcataa
     1321 tttcgggtaa taaaaaactt acccttcata atgccttgtt gttccacccc aaaatcgggg
     1381 ttgaaacaac aaaagataat atagttatat ccgggtggaa attaatgtca atatttgaaa
     1441 tgtggagaaa atgagaataa aaaaatccct tagaaaatag aatttttttc tattttttaa
     1501 gggatttggc ccgtccggcg aggacggttt taagcatatt ttgagcgaag aatttttaaa
     1561 ttttcttctt gttgtttttc ttcttcttta attttaattt ctttaacaaa tcttgcacga
     1621 tcgccattga aaatgatcgc cgggttaata taaaaaatgt atctttttac agattttgcg
     1681 atcattttct tttctaataa ttcttttaaa cctctatagt agacagcttt tgaaattgct
     1741 gactcgtatt tttcagcttc ttcttttgct gtttcgaaat ttaaataaat ttcgtcaccg
     1801 cctatctgtt tttgatacaa acccataatt atgaaaaaca atttaaatcc ggtttgagac
     1861 aaatcaaaat agaatttgat ttgtcctgta aaaattttta aaaattcttc cctatcaact
     1921 tcttgaattt gtgatattgt tgttgtgaaa accccctctt ctgtttctac ttctgaaccc
     1981 cgatcaacag ttagtttttt ctttttagta tcaatagaca tattgaaaac aaacgggttc
     2041 tttttatgtt cgatctcact atcgttttca atattagttt tttctttgtg taaaatttct
     2101 acattgtttt tctttgtcat atacaaaccc tctctgttta aaaaataacc gaaaaaattc
     2161 tcactcggtg agactcttct tctcattttt taacagattt ttgagactag tcaagaaaac
     2221 acattggatt tattctcatt ttttacaaaa aaaatgagac aaaaagtcac aatatcgaga
     2281 catttagtca ctgtagagag aattttttct ctcctgagag aatttttttt ctctatagag
     2341 actagacatc cagtgtttat gcgggttacg agacatataa gaatataaga aatataagat
     2401 ctaagaggct ttgcttagct ctgagtaaaa tttttatcgg cattgtgata actgggcttc
     2461 gcaatagaaa accaaaaacc atctggaaaa ccatcccagt taccccgatt ccggggccct
     2521 gcttcgcaat atggaggatt ttgctataaa aaccgtcctg gccggacggg ccaacccttc
     2581 aaagccatca ggcgaagcct gacagctttt cagcgtttta atcgacggac gggaggtgga
     2641 cttgctacat ctggaggggt ggaaatagcc gcgatttttg cggctgtctg ctgtttgctt
     2701 gcaattttga accttaacaa tctatcgccc ccacggggct tagagcttgt ttcaacatct
     2761 gtaatgtcgt caaatccacc tgcgcttctt ttgttctttc taccgtagct ttgttgtgat
     2821 ctgacagaag catgacccat aattttagcg attttttcag catcaaaacc ctctgaattt
     2881 ttcaattctg tcg
//
