LOCUS       CP055461               11857 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00489 plasmid pRHBSTW-00489_4,
            complete sequence.
ACCESSION   CP055461
VERSION     CP055461.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148633
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00489
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.108404575331x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 17:26:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,652
            Genes (coding)                    :: 4,435
            CDSs (with protein)               :: 4,435
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 90 of 217
            Pseudo Genes (incomplete)         :: 115 of 217
            Pseudo Genes (internal stop)      :: 31 of 217
            Pseudo Genes (multiple problems)  :: 17 of 217
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00489"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00489_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(11695..11857,1..860))
                     /locus_tag="HV216_23730"
     CDS             complement(join(11695..11857,1..860))
                     /locus_tag="HV216_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="QLO50006.1"
                     /translation="MTCLFAPSLVVEDVTTQNVLATFIGSFLFSLVGIIALNMGAYGER
                     GRVILFIVTLVVIALIIITLLRWIQHLTSLGRVGETTAKVEQAAIETFIARAKSPCLGG
                     YPWLENNEQPKGTVAVYPEKIGYVEYIDMVKLGSLLSNDPRHVYLVAQPGSFIHPSMPV
                     LYLSSGQESSICTDLLETIIVSDVRSFAQDPRFCLSVMAEIACRALSPAVNDPGTAIDV
                     IGRGVRILSAYAQNKIDETEVIYPSVHVAPLQIKDLLEDFFLPVARDGASMREIQIRVL
                     KGLSMLSKGWPGMFAEAAHILASETLEHATRTGHIAHDEYLIKSIYYNLFSGENPNKKP
                     "
                     /name="DUF2254 domain-containing protein"
     gene            complement(984..1964)
                     /locus_tag="HV216_23735"
     CDS             complement(984..1964)
                     /locus_tag="HV216_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="QLO49996.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            2150..3181
                     /locus_tag="HV216_23740"
     CDS             2150..3181
                     /locus_tag="HV216_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001898375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="QLO49997.1"
                     /translation="MKITLTPQQKLQLEQMHDIERDSRVCDRIKAVLLASEGWSQTMIS
                     QALRIHESTVARHLSDYVLSEKLKPENGGSQSKLSATQTMHLIEHLTERTYPHTHQIVA
                     YVKETFGLDYTVSGMNKWLHHNGFSYKQPKGVPHKFDEAKQQAFIDAYEALKASCDEDE
                     SIVFIDAVHPTLSTKISHGWIRTGQDKVIETTGNRSRLNILGALNLSDIGATIIDDYES
                     INSETIVRFFCKLRESYPLAHKLHIILDGAGYHRSDLVRDAAFVLNIELHYLPPYSPNL
                     NPIERLWKVMNEKSRNNVYFKSKRDFKAAIDQFFTVTLPEIAGSLASRINDNFQVLKPA
                     SSS"
                     /name="IS630 family transposase"
     gene            3287..3895
                     /gene="mrkA"
                     /locus_tag="HV216_23745"
     CDS             3287..3895
                     /gene="mrkA"
                     /locus_tag="HV216_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 fimbria major subunit MrkA"
                     /protein_id="QLO49998.1"
                     /translation="MKKVLLSAAMATAFFGMTAAHAADTTVGGGQVNFFGKVTDVSCTV
                     SVNGQGSDANVYLSPVTLTEVKAAAADTYLKPKSFTIDVSKCQAANGTKQDDVSKLGVN
                     WTGGNLLAGATSKQQGYLANTEASGAQNIQLVLSTDNATALTNKIIPGGSTQPKAKGDA
                     SAVADGARFTYYVGYATSAPTTVTTGVVNSYATYEITYQ"
                     /name="mrkA"
     gene            3991..4692
                     /gene="mrkB"
                     /locus_tag="HV216_23750"
     CDS             3991..4692
                     /gene="mrkB"
                     /locus_tag="HV216_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 fimbria chaperone MrkB"
                     /protein_id="QLO49999.1"
                     /translation="MKRIALFFCFIFSFAAHANNIIVNGTRFIYPGNEKEITVQLSNNA
                     DRPALAQAWLDNGDADATPDTITTPFIITPPISRVDAKSGQTLRIKLGSSAGLAKDKET
                     LWWLNLLEIPPVVANQKNEDQNVLQLAIRSRFKFIYRPAGLGNRDAAAEKLTLTASGSS
                     LAINNPTPFYITVSRISRDGGKALNSKTVMLAPQSSQTVALSSAVNRGETLTVNNINDY
                     GADVAVKVAVK"
                     /name="mrkB"
     gene            4704..7190
                     /gene="mrkC"
                     /locus_tag="HV216_23755"
     CDS             4704..7190
                     /gene="mrkC"
                     /locus_tag="HV216_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 fimbria usher protein MrkC"
                     /protein_id="QLO50000.1"
                     /translation="MKQRSFCPGRLSTAIAIALCCFPPFSSGQENPGTVYQFNDGFIVG
                     SREKVDLSRFSTSAITEGTYSLDVYTNDEWKGRYDLRIARDKDGRLGVCYTKAMLAQYG
                     IAAEKLNPQLSEQEGYCGSLKSWRNEENVKDNLVQSSLRLNISVPQIYEDQRLKNYVSP
                     EFWDKGITALNLGWMANAWNSHTSSVGGSDNSSAYLGVNAGLSWDGWLLKHIGNLNWQQ
                     QQGKAHWNSNQTYLQRPIPQLNSIVSGGQIFTNGEFFDTIGLRGVNLSTDDNMFPDGMR
                     SYAPEIRGVAQSNALVTVRQGSNIIYQTTVPPGPFTLQDVYPSGYGSDLEVSVKEADGS
                     VEVFSVPYASVAQLLRPGMTRYALSAGKVDDSALRNKPMLYQATWQHGINNLLTGYTGV
                     TGFDDYQAFLVGTGMNTGIGALSFDVTHSRLKSDAHDDSGQSYRATFNRMFTDTQTSIV
                     LAAYRYSTKGYYNLNDALYAVDQEKNSRSNYTLWRQKNGMTFTVNQNLPDGWGGFYLSG
                     RISDYWNRSGTEKQYQVSYNNSFGRLSWSASAQRVYTPDSSGHRRDDRISLNFSYPLWF
                     GDNRTANLTSNTSFNNSRFASSQIGINGSLDSENNLNYGVSTTTATGGQHDVALNGSYR
                     TPWTTLNGSYSQGEGYRQSGIGASGTMIAHSGGVVLSPESGSTMALIEAKDAAGAMLPG
                     SPGTRVDSNGYAILPYLRPYRINAVEIDPKGSHDDVAFDRTVAQVVPWEGSVVKVAFGT
                     KVQNNLTLQARQANHEPLPFAASIFSPDGKEIGVVGQGSMMFISDANAKRAIVKWSGGQ
                     CSVDLGQQTTKDSVCR"
                     /name="mrkC"
     gene            7181..8176
                     /gene="mrkD"
                     /locus_tag="HV216_23760"
     CDS             7181..8176
                     /gene="mrkD"
                     /locus_tag="HV216_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 fimbria adhesin subunit MrkD"
                     /protein_id="QLO50001.1"
                     /translation="MSLRKLLTLFIVLMALGTTSSWASCTRLSSPTVMLDMVVGRVVVP
                     SDLPVGSVILTRDWTMSAPGGASYRCTSGTNRFAAKIVSPGATDLGNKIYSTNVPGIGM
                     RFSRGGATVNIVYPDVFSSQVYNTTYYSLEGSRFTLEIIKTAATTGSGTLAAGKYTSYD
                     WESGDNPILETYLSANAITVVSPSCSVLSGKNMNVDVGSIRRTDLKGVGTTAGGKDFNI
                     DLQCSGGLSETGYANISTSFSGTLATSTTATMGALLNEKAGSGMAKGIGIQVLKDGSPL
                     QFNNKYTVGRLNNQETRYITIPLHARFYQYGPTTSTGEVESHMIFNLTYD"
                     /name="mrkD"
     gene            8190..8825
                     /gene="mrkF"
                     /locus_tag="HV216_23765"
     CDS             8190..8825
                     /gene="mrkF"
                     /locus_tag="HV216_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 fimbria minor subunit MrkF"
                     /protein_id="QLO50002.1"
                     /translation="MKGLPKNTIAWLLFCGSLAAPSAWGFETNYDRGRVDFAGRVTDIS
                     CSIALNGGQHAGSGNVWLAPVSLAEVHDRGAGAFMKPQPFTLALSNCQLRHDGGAASQD
                     EVRRVSVRWVDGFLLTAVGNENAGYLANTLPDGAQNIYLALSTNDNNTLDKSNKIVPAD
                     PQQNQVRLQESAVSGGLFTYYVGYVSPTPKSATSGPITSWATWELVYN"
                     /name="mrkF"
     gene            complement(8934..10472)
                     /locus_tag="HV216_23770"
     CDS             complement(8934..10472)
                     /locus_tag="HV216_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISKpn24 family transposase"
                     /protein_id="QLO50003.1"
                     /translation="MNSLLPDDIDELKRLLAEQEALNRALLEKLNEREREIDHLQAQLD
                     KLRRMNFGSRSEKVSRRIAKMEADLKQLQKESDTLTGRVDDPAVQRPLRQTRTRKPFPE
                     SLPRDEKRLLPAASCCPECGGALSYLGEDAAEQLELMRSAFRVIRTVREKHACTQCDAI
                     VQAPAPSRPIERGIAGPGLLARVLSSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDA
                     CCRLLSPLEGALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSELA
                     PAVWFAYSPDRKGIHPQSHLAGFSGVLQADAYAGFNELYRNGQITEAACWAHARRKIHD
                     VHVRTPSALTEEALKRIGELYAIEAEIRGMPAKRRLAERQQKAKPRLKSLESWLREKVK
                     TLSRHSELAKAFTYVLNQWPALAYYTDDGWAEADNNIAENALRMVSLGRKNYLFFGSDH
                     GGERGALLYSLIGTCKLNGVEPESYLRHVLDVIADWPINRVSELLPWRVALPNE"
                     /name="IS66-like element ISKpn24 family transposase"
     gene            complement(10521..10868)
                     /gene="tnpB"
                     /locus_tag="HV216_23775"
     CDS             complement(10521..10868)
                     /gene="tnpB"
                     /locus_tag="HV216_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="QLO50004.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVFI
                     FRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDWRQ
                     PKRLLTSLTML"
                     /name="tnpB"
     gene            complement(10865..11269)
                     /locus_tag="HV216_23780"
     CDS             complement(10865..11269)
                     /locus_tag="HV216_23780"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="QLO50005.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGINDN
                     LLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPATSPRSQPVAAAAPESLNIS
                     CEVTFRHGSLRLNGAISENILNLLIRELKR"
                     /name="IS66 family insertion sequence hypothetical protein"
     gene            complement(11350..11487)
                     /locus_tag="HV216_23785"
                     /pseudo=""
     CDS             complement(11350..11487)
                     /locus_tag="HV216_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /name="IS5/IS1182 family transposase"
ORIGIN
        1 agcatagaca gtcctttaag gactcttatc tgaatctccc tcatactcgc accatcacgt
       61 gcaacaggta agaaaaagtc ttccagtagg tctttgatct gaagtggcgc aacatgtact
      121 gaaggatata tcacttcagt ttcatctatt ttattctgcg cgtaagcgga gagtattcga
      181 acacctctgc caatgacatc aatcgcagta ccgggatcgt ttactgcggg cgaaagggcc
      241 ctgcaggcta tttcggccat gacgctgaga cagaatcggg gatcctgagc aaatgaacgc
      301 acatccgaga caatgatcgt ttcaagtaaa tcggtacaga ttgatgactc ctgaccagaa
      361 ctcaggtaca aaactggcat cgatggatgt atgaaactgc ctggctgcgc cacaagatat
      421 acatgacgag gatcattgct cagcagactg ccgagcttga ccatgtcaat atattcaaca
      481 tagccaattt tctccggata tacggcaaca gttccttttg gttgttcatt gttctcaagc
      541 catggatatc cgccaagaca tggacttttt gctctcgcaa taaatgtctc gatcgccgcc
      601 tgttctactt ttgctgttgt ctcacctacc ctgcccagag aggtcaaatg ctgtatccag
      661 cgaagcaatg tgatgattat taaggcaatg acaaccagtg ttacaatgaa taaaatgact
      721 ctaccccttt ctccgtaggc tcccatattg agggcaataa tccctaccag gctgaagaga
      781 aaagaaccga taaaagtggc cagtacattt tgtgtggtta cgtcttctac aactaaggaa
      841 ggtgcgaata agcaggtcat gtcttcccaa gctgactcgt tgattaaaat ttcgcggatc
      901 tgggccgatt tttttcccgc aaacacatcg aatcagccta tttaggctat tttttccacc
      961 atttctggcg ttatttccgg tttttactga gatctctccc actgacgtat catttggtcc
     1021 acccgaaaca ggttggccag ggtgaataac atcgccagtt ggttatcgtt tttcagcagc
     1081 cccttgtatc tggctttcac gaagccgaac tgccgcttga tgatgcgaaa cgggtgctcc
     1141 accctggcac ggatgctggc tttcatgtat tcgatgttga tggccgtttt gttcttgcgc
     1201 ggatgctgct tcaaggtttt taccttgccg ggacgctcgg cgatcagcca gtccacatcc
     1261 acctcggcca gctcctcgcg ctgtggcgct ccttggtagc cggcatcggc tgagacaaat
     1321 tgctcctctc catgaagcag attacccagc tgattgaggt catgctcgtt ggccgcggtg
     1381 gtgactaggc tgtgggtcag gccactcttg gcatcgacac caatgtgggc cttcatgcca
     1441 aagtgccact gattgccttt cttggtctga tgcatctccg gatcgcgttg ctgctctttg
     1501 ttcttggtag agctgggtgc ctcaatgatg gtggcatcca ccaaagtgcc ttgggtcatc
     1561 atgacgcctg cttcggccag ccagcgattg atggtcttga acaattgacg ggccagttga
     1621 tgctgctcga gcaggtggcg gaaattcatg atggtggtgc gatccggcag ggcgctatcc
     1681 agggataatc gggcaaacag gcgcatggag gcgatttcgt acagggcatc ttccatggca
     1741 ccgtcgctca ggttgtacca atgctgcatg cagtgaatac gcagcatggt ctccagcgga
     1801 tagggccgtc ggccattgcc cgccttggga taaaacggct cgatgacagc ggtcatattc
     1861 tgccatggca gaatctgctc catgcgggag aggaaaatct cttttcgggt ctgacggcgc
     1921 ttagtgctga attcactatc ggcgaaggtg agttgatggc tcatgatgtc cctctgggat
     1981 gcgctccgga tgaatatgat gatctcatat caggaacttg ttcgcacctt ccttaggttc
     2041 gttacctgac gccttaaata ttgcggctcc cggtcagtcg aagattctat tatacccgtg
     2101 gcgattcaga tgcttgattt tttttagaat caggttcacg ctaggaacca tgaaaattac
     2161 actgactcct caacagaaac tgcaactcga acaaatgcac gatattgaac gtgatagtcg
     2221 agtttgcgac cgtattaagg ctgttttgct ggcttctgaa ggctggagtc agaccatgat
     2281 ttcacaagct cttcggattc atgaatccac cgttgcacgt caccttagcg actacgttct
     2341 ttctgaaaaa cttaagcctg aaaatggcgg aagtcaaagc aagctttctg caactcaaac
     2401 catgcaccta atcgagcatt tgactgagag aacctatcct catactcatc aaattgtcgc
     2461 ctacgttaaa gagacttttg gacttgatta cactgtctct ggtatgaaca aatggcttca
     2521 ccataacggt tttagctaca agcaaccgaa aggcgttcca cataagtttg atgaagcaaa
     2581 acaacaagct ttcatagatg cttacgaagc gctaaaggca agttgtgacg aggatgaatc
     2641 gatagtcttt atcgatgctg ttcacccaac cctatcaaca aaaatatctc atggctggat
     2701 acgaactggt caagataaag tgattgaaac aacgggaaat cgcagtcgat tgaacattct
     2761 tggcgcactg aacctgtcag atatcggagc tactattatt gatgattatg agagcattaa
     2821 cagtgaaacg atagttcgct ttttctgtaa gttaagagag agttatccgt tagctcataa
     2881 gcttcatatc attttagatg gtgcgggata tcaccgtagt gacttggtca gagatgcggc
     2941 atttgtcctt aatatcgagc tgcattatct tccaccttac agcccaaacc tcaatccaat
     3001 agagcggctt tggaaagtaa tgaatgagaa gtcgaggaac aacgtttact tcaaaagtaa
     3061 acgggacttc aaggcggcaa tagaccaatt ttttactgtg actcttccag agatcgcagg
     3121 ctctttggca tctcgaatta atgataattt tcaggttctc aagcctgcat cttcaagttg
     3181 attcggtata gcagtggcat aaatcagcat cgcctgatgt ttctggttct atgcgaattc
     3241 acagtgtgct cattgattcg taattcactc tgacaaggaa atggctatga aaaaggttct
     3301 tctctctgca gcaatggcaa ccgcgttttt tggcatgact gctgcccatg cagctgatac
     3361 cactgttggc ggcggccagg ttaatttctt cggtaaagtt accgatgtat cctgtactgt
     3421 ttccgttaac ggccagggca gcgatgcgaa cgtttacctg tctccggtaa ccctgactga
     3481 agttaaagcg gcagcagcgg atacttacct gaaaccgaaa tctttcacca ttgacgtctc
     3541 taagtgccag gctgctaatg gcactaaaca ggatgacgta agcaaactgg gcgtgaactg
     3601 gaccggcggt aacctgctgg ctggcgcaac cagcaaacaa cagggctacc tggcgaacac
     3661 cgaagcttcc ggtgctcaga acatccagct ggtgctgtct actgacaacg ctaccgcgct
     3721 gaccaacaaa atcatcccgg gtggcagcac tcagccgaaa gcgaaaggcg acgcttccgc
     3781 tgtagctgat ggcgcgcgtt tcacctacta cgtaggctac gccaccagcg cgccgaccac
     3841 ggtaaccacc ggtgtggtta acagctacgc gacttacgaa attacttacc agtaattccg
     3901 tcagaaaata aaactgcccc gcgatggggc agttaataac aataataaag cggcaatgtc
     3961 cgccgcttgt atttttattc tgaggtcagc atgaagcgta ttgccctttt tttctgtttc
     4021 atttttagtt ttgcggccca tgccaacaat attattgtta acggtacccg ctttatttat
     4081 ccaggaaatg aaaaagaaat aacggtacag ctttcaaata acgccgaccg tccggcgctg
     4141 gcgcaggcct ggctggataa cggcgatgcc gatgccacac cggataccat taccaccccg
     4201 tttattatca ccccgccgat ttcgcgcgtt gacgccaaga gcggccagac gctgcgtatt
     4261 aagctcggta gcagcgccgg tctggcgaaa gataaagaga cgctgtggtg gctgaatctg
     4321 ctggaaattc cgccggtggt ggccaatcag aaaaatgaag accagaacgt gctgcagctg
     4381 gcgatccgtt cgcgctttaa atttatttat cgtcctgccg gactgggcaa ccgcgatgcg
     4441 gcggccgaga agctgaccct gaccgccagc ggcagtagtc tcgccatcaa caacccgacg
     4501 ccgttctaca ttaccgtcag ccgtatttcc cgcgacggcg gtaaagccct gaacagtaaa
     4561 accgtgatgc tggcgccgca gtccagccag acggtggcgc tctcctccgc cgtcaaccgc
     4621 ggcgaaaccc tgaccgttaa caacattaat gactatggcg ccgatgtcgc agtgaaagtg
     4681 gccgttaaat aagggaaaag ctaatgaagc agaggtcatt ctgcccggga agattaagca
     4741 ccgcgatcgc catcgcgctg tgctgctttc cgccattttc cagcggacag gaaaacccgg
     4801 gaacggtata tcagtttaac gacggcttta ttgtcggcag tcgcgaaaag gtcgatctgt
     4861 cgcgcttttc gaccagcgcg attaccgaag gcacttactc ccttgacgtc tacaccaacg
     4921 acgagtggaa agggcgctac gatttacgca tcgcgcgcga taaagatggt cggctgggcg
     4981 tctgctacac caaagcgatg ctggcccaat atggcatcgc ggcggaaaag ctcaatccgc
     5041 agctgagcga gcaggagggc tactgcggta gcctcaaatc ctggcgcaac gaagaaaacg
     5101 tcaaagacaa cctggtccag tcctccctgc ggttgaacat ttcggtaccg cagatctatg
     5161 aagatcaacg cctgaaaaac tatgtcagcc cggagttctg ggacaaaggt attaccgcgc
     5221 tcaatcttgg ctggatggcg aacgcctgga acagccatac ctcttcggta ggcgggtcgg
     5281 ataacagcag cgcctatctg ggcgtcaacg ccggtctgtc gtgggacggc tggctgctga
     5341 agcacatcgg caacctgaac tggcagcagc agcagggtaa agcgcactgg aacagcaacc
     5401 agacctatct ccagcgccct atcccacagc tcaactccat cgtcagcggc gggcagattt
     5461 tcaccaatgg tgaatttttc gacactatcg gtctgcgcgg ggttaatctt tccaccgatg
     5521 acaatatgtt cccggacggc atgcgctcgt atgctccgga aattcgcggc gtggcgcaga
     5581 gcaacgccct cgtcaccgtg cgccagggca gcaatattat ctatcagacc accgttccgc
     5641 ccggaccgtt taccctgcag gatgtctatc cttccggcta tggcagcgat cttgaggtct
     5701 cggtgaaaga agcggatggc tcggtagaag tgtttagcgt gccttacgct tccgtcgccc
     5761 agctgctgcg tccgggaatg acccgctacg cgctctccgc cggtaaagta gacgacagcg
     5821 cgctgcgcaa caagccgatg ctctatcagg cgacctggca gcacgggatc aacaatctgt
     5881 tgaccggcta caccggggtc accggttttg acgactatca ggccttcctg gtggggacag
     5941 ggatgaacac cggcatcggc gcgctctctt ttgacgtcac ccattcgcgg ttgaagagcg
     6001 atgcccatga tgactcgggt cagagctatc gcgcgacctt taaccgcatg tttaccgata
     6061 cccagaccag catcgtgttg gcggcgtatc gctactcgac gaaaggttat tacaacctca
     6121 acgacgcgct gtacgccgtc gatcaggaaa aaaatagtcg cagcaactac accctgtggc
     6181 ggcaaaaaaa cggcatgacc ttcacggtga accagaacct gccggacggc tggggcgggt
     6241 tttatctcag cggccgtatc tccgattact ggaaccgctc cgggaccgag aagcagtatc
     6301 aggtcagcta caacaattca tttggtcgtc tctcctggtc ggcgagcgcg cagcgcgtct
     6361 atacgccaga cagctccggt catcgtcgcg acgatcgtat ttcactcaac ttcagctacc
     6421 cgctgtggtt tggcgataac cgcaccgcca acctgacgtc gaacacctcg ttcaataact
     6481 cgcgctttgc cagctcgcag attggtatca acggttcgct ggacagtgaa aacaacctta
     6541 actacggcgt gtcgaccacc accgccaccg gcggtcagca tgatgtggcg ctcaacggca
     6601 gctaccgtac gccatggacc accctcaacg gcagctacag ccagggcgaa ggctatcgtc
     6661 agagcggcat tggcgccagc gggacgatga tcgcccacag cggcggcgtg gtgctctcac
     6721 cggaaagcgg ctcgacgatg gccctgattg aagccaaaga cgcggccggg gcgatgcttc
     6781 cgggatcgcc gggcacccgt gtggacagca atggttacgc catcctgccg tatctgcgcc
     6841 cgtaccggat taacgccgtg gaaatcgacc cgaaaggcag ccatgacgac gtggccttcg
     6901 atcgcaccgt ggcgcaggtt gtgccgtggg aaggcagcgt ggttaaggtc gcgttcggca
     6961 ctaaggtgca gaacaacctc accctgcagg cgcgccaggc gaaccacgag ccgttgccgt
     7021 ttgcggcaag tattttctcc cccgacggca aggagatcgg cgttgtcggc cagggcagca
     7081 tgatgtttat cagcgatgcg aacgccaaac gcgcgattgt gaagtggagc ggcgggcagt
     7141 gttcggtgga tctgggacag caaacaacaa aggatagcgt atgtcgctga ggaaattact
     7201 aacgcttttt atcgtcttaa tggcgctggg aaccacctcg tcctgggcct cctgtacgcg
     7261 tctctcatcg ccaacggtga tgctggacat ggtggtgggc agggtggtgg tgccctcgga
     7321 tctgccggtg ggctcggtga tccttacccg cgactggacg atgagcgctc ccggcggggc
     7381 aagctatcgc tgtacttccg gcaccaaccg ctttgcggcg aagatcgtgt cgccaggggc
     7441 cacggacctt ggaaataaaa tctactccac caacgtgccg ggaatcggca tgcgtttcag
     7501 ccgcggcggc gcaacggtaa atatcgtcta tcccgacgtc ttttcgtccc aggtatataa
     7561 caccacctac tattccctcg aagggtcgcg ctttacgctg gagattatca aaaccgcggc
     7621 aaccaccggc agcggcaccc tggcggcggg taagtacacc tcctacgact gggagagcgg
     7681 cgataacccg atcctcgaaa cctatctgag cgccaacgcc atcaccgtgg tctcgccctc
     7741 ctgttcagtg ctgagcggga aaaatatgaa tgtcgatgtg ggttccatcc ggcgcaccga
     7801 cctgaaaggg gtcggcacca ccgccggcgg gaaggatttt aatatcgacc tgcagtgcag
     7861 cggcggcctg agtgaaacgg gatatgccaa cattagcacc tcgttctccg gtaccctggc
     7921 caccagcact accgctacca tgggcgcctt gctgaatgaa aaagccggca gcgggatggc
     7981 gaaaggcatt ggcatccagg tgctgaagga tggctccccg ctgcagttta ataataaata
     8041 caccgtcggc cgcttgaata atcaggagac ccgctacatc accataccgc tgcacgcgcg
     8101 tttttatcag tatggcccga cgaccagcac cggcgaggtg gagtcgcata tgatctttaa
     8161 cctgacgtac gattaatacg ggagggggaa tgaagggatt gccgaaaaac actatcgcgt
     8221 ggctgctatt ttgcggcagc ctcgccgccc cctcggcgtg gggttttgag accaattacg
     8281 atcgcggacg ggtcgatttt gccgggcggg tgacggatat ttcctgtagc atcgcgctaa
     8341 acggcggcca gcatgccggc agcggtaatg tctggctggc gccggtcagc ctggcggaag
     8401 tccacgatcg cggtgccggg gcgtttatga aaccgcagcc gtttaccctg gcactgtcaa
     8461 actgccagct gcgccatgat ggtggggcag cctcgcagga tgaggtgcgt cgggtcagcg
     8521 tgcgctgggt cgatggtttt ttactgacgg cggtcggtaa cgaaaacgcc gggtatctgg
     8581 ccaatacctt gccggatgga gcgcagaata tttatctggc gctatcgact aacgataata
     8641 atacgctgga taagagtaat aaaattgtcc cggcggaccc gcagcaaaat caggtgcgtt
     8701 tgcaggaaag cgcggtcagc ggcggcctgt tcacctatta cgttggctat gtttcgccaa
     8761 cgccgaaaag cgccaccagc gggccgatta ccagctgggc aacgtgggaa ttagtttata
     8821 attaagtgac gttaaaaagc cgggaattcc cggctttttt tacatggcaa tatcatcggc
     8881 gaccagttgt tcaatagtaa gcgtgcagcg agaaccgtat tgacggggat gtgttattca
     8941 tttggcaatg ctacgcgcca ggggagcagt tcgctgaccc ggtttatcgg ccagtcagct
     9001 ataacatcaa gcacatggcg aaggtagctt tctggctcca ctccgttcag tttgcatgtc
     9061 ccgatcaggc tgtacagcag cgctccccgc tctcctccat gatccgaacc gaagaacagg
     9121 tagtttttgc ggcccaggct gaccatccgt agcgcattct cagctatgtt gttatctgcc
     9181 tcggcccagc cgtcgtcagt atagtaagcc agcgccggcc actggttcag tacgtacgtg
     9241 aacgctttcg ccagttctga gtgtcgcgac agcgttttca ccttttcacg cagccagctt
     9301 tccagggatt tcagccgcgg tttagctttt tgctgacgtt ctgcaaggcg tcgcttcgcc
     9361 ggcatccccc ttatttccgc ctcgatggca tataactcac cgatccgttt cagggcttcc
     9421 tccgtcagcg ctgacggggt gcgaacgtgc acatcgtgga tcttgcggcg ggcatgagcc
     9481 cagcaggcag cttccgttat ctgtccattg cggtacagtt cgttgaaccc ggcgtacgca
     9541 tccgcctgca gaacaccgct gaagccagcg agatggctct gcgggtggat accttttctg
     9601 tccgggctgt aagcgaacca cactgccggc gccagctctg acccggcgtt acggtcgtca
     9661 cgaacgtacg tccacaaccg cccggtcttc gttttcttat tacctggcaa cagcaccggg
     9721 acaggcgtgt catcagcatg gagcttaccg tcagtcagca catagtcctg aagcgcccct
     9781 tccagcggtg acagtagccg gcaacacgca tccacccagc ccgacagcag tgagcggctc
     9841 agctccacac cctggcggcc gtatatttca gactggcggt acagcggggt gtgctctgca
     9901 tactttgaac tcagcacgcg ggccagcagc cccggtcctg cgataccccg ctcgatgggc
     9961 cgtgaaggcg cgggggcctg cacgatggca tcgcactgag tacaggcatg cttttcacgt
    10021 actgtccgga taacccggaa ggcgctgcgc atcagctcca gctgttcggc ggcatcttca
    10081 cccaggtaac tcagcgcacc accacattcc gggcagcatg acgctgccgg cagcagccgt
    10141 ttttcgtcac ggggaagtga ttcagggaac ggtttgcggg tacgggtctg ccgcagcggg
    10201 cgctgcacgg ccgggtcatc cacccggccg gtaagggtat cgctttcttt ctgcaactgc
    10261 ttaaggtcgg cttccatctt cgcgatacgg cgggagactt tttcggagcg gctgccgaag
    10321 ttcatccggc gcagcttatc cagttgcgcc tgcaggtgat ctatttcgcg ttcacgctcg
    10381 ttcagctttt ccagaagggc acggttcagc gcctcctgtt cggcaaggag acgtttcagt
    10441 tcatcgatat cgtcaggaag taagctgttc ataccgggta tattaccagg ctcattcagc
    10501 gtcgaccagg ataaagaggc ctacaacata gtcagtgacg taagcagtct tttaggctgc
    10561 cgccagtcga taccttcaag gagcatcgcc agctgtgccg gtgtgaggaa cactttgcca
    10621 tcccgggctg acggccaggc gaagcggccg cgctccagcc gtttggtcag cagacacagt
    10681 ccatcgccgg tagaccagag gagctttacc tgactgccat tacgcccacg gaagataaaa
    10741 acgtgacctg acatcggatc gtctttcagc gtcgtctgca cctttgccgc caggccgttg
    10801 aagccgtttc tcatatcggt gatgccagcg accagccaga tctttgtccc tgatggtaat
    10861 gggatcaacg tttgagctcc cgtatcagca ggttcaggat attttcgctg atggcaccat
    10921 tcagacggag tgatccgtgc cggaacgtca cttcacagct gatattgagt gattcaggtg
    10981 ccgcagcagc tacaggttgt gaccgggggg aggttgcagg gacaacatct tcggcatcaa
    11041 gtgttatcgg gagcagctca ggcacgttgt tttctgttgt tgaaggagga cgtagttttc
    11101 cttcgcgcca gtactggcgc cacttaaaca gcaaattatc gttgatccca tgctcacgag
    11161 cgagttgcgc tacggagatc tctggtcgat gcgagagttc aaccattttg attttgaact
    11221 caacgggata attagggctt tttttacgca ctgcggttaa tgatttcatg gatagcgtcc
    11281 accatatttg gtgtccatta tcctctcagg aatttcagga tctgccagac ggtgctgaga
    11341 cgacgcttac ggataaaacg gctcgatgac agcggtcata ttctgccatg gcagaatctg
    11401 ctccatgcgg gagaggaaaa tctcttttcg ggtctgacgg cgcttagtgc tgaattcact
    11461 atcggcgaag gtgagttgat ggctcatgat gtccctctgg gatgcgctcc ggatgaatat
    11521 gatgatctca tatcaggaac ttgttcgcac cttccttagc tacgtccgct tcacgctgtg
    11581 agttcaataa ttgtgttgcg tccatcagtt gaaatcacag ctcaaagtag acgctcattc
    11641 tcactggaat gctttgcttt caccttgcca gaggacaaat cctttgtccc gcaactatgg
    11701 ttttttatta ggattttcgc cactgaataa attataataa attgatttta taagatattc
    11761 gtcatgggct atatgaccag tacgagttgc atgctctaat gtttcagatg ccagtatatg
    11821 tgcggcttca gcaaacatcc caggccaacc cttactc
//
