LOCUS       CP054461                6988 bp    DNA     circular BCT 14-JUN-2020
DEFINITION  Escherichia coli strain SCU-103 plasmid pSCU-103-4, complete
            sequence.
ACCESSION   CP054461
VERSION     CP054461.1
DBLINK      BioProject: PRJNA624897
            BioSample: SAMN14943534
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Hartman,A., Gonzalez,A.,
            Gill,M., Pandori,M. and Hess,D.
  TITLE     Title: F plasmids are the major carriers of antibiotic resistance
            genes in human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Hartman,A., Gonzalez,A.,
            Gill,M., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye, Unicycler v. 2.6, 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/04/2020 20:11:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,482
            CDSs (total)                      :: 5,353
            Genes (coding)                    :: 3,705
            CDSs (with protein)               :: 3,705
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 1,648
            CDSs (without protein)            :: 1,648
            Pseudo Genes (ambiguous residues) :: 0 of 1,648
            Pseudo Genes (frameshifted)       :: 1,488 of 1,648
            Pseudo Genes (incomplete)         :: 174 of 1,648
            Pseudo Genes (internal stop)      :: 59 of 1,648
            Pseudo Genes (multiple problems)  :: 68 of 1,648
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            NCBI analysis of this genome has determined that >30% of the
            predicted CDS features contain frameshifts, which is well above the
            usually observed value of <10%.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-103"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-103-4"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            complement(222..455)
                     /gene="mbeD"
                     /locus_tag="HPE39_27325"
     CDS             complement(222..455)
                     /gene="mbeD"
                     /locus_tag="HPE39_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QKQ01907.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="mbeD"
     gene            complement(462..1022)
                     /locus_tag="HPE39_27330"
     CDS             complement(462..1022)
                     /locus_tag="HPE39_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QKQ01908.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="mobilization protein"
     gene            complement(994..1737)
                     /locus_tag="HPE39_27335"
                     /pseudo=""
     CDS             complement(994..1737)
                     /locus_tag="HPE39_27335"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1700..2023)
                     /locus_tag="HPE39_27340"
     CDS             complement(1700..2023)
                     /locus_tag="HPE39_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QKQ01909.1"
                     /translation="MLTIRVTDEEHARLLARCEGTQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2091..2282
                     /locus_tag="HPE39_27345"
     CDS             2091..2282
                     /locus_tag="HPE39_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QKQ01910.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3288..3419)
                     /locus_tag="HPE39_27350"
     CDS             complement(3288..3419)
                     /locus_tag="HPE39_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QKQ01911.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            3449..3739
                     /gene="cki"
                     /locus_tag="HPE39_27355"
     CDS             3449..3739
                     /gene="cki"
                     /locus_tag="HPE39_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="QKQ01912.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(3736..5382)
                     /gene="cka"
                     /locus_tag="HPE39_27360"
     CDS             complement(3736..5382)
                     /gene="cka"
                     /locus_tag="HPE39_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="QKQ01913.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEDNSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFITLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            5623..5913
                     /locus_tag="HPE39_27365"
     CDS             5623..5913
                     /locus_tag="HPE39_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKQ01914.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(5905..6183)
                     /locus_tag="HPE39_27370"
     CDS             complement(5905..6183)
                     /locus_tag="HPE39_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKQ01915.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            6305..6727
                     /gene="exc2"
                     /locus_tag="HPE39_27375"
     CDS             6305..6727
                     /gene="exc2"
                     /locus_tag="HPE39_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QKQ01916.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     Typing          complement(2368..2455)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2466..3022
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(116..1710)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2083..2285)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="100.0"
                     /evalue="3.73e-11"
ORIGIN
        1 atctggcaaa gaggcaaaac acagcaacaa aaacgacacg ccagaatcgc gcccggatgc
       61 gtttttaacg cgttccggta ccatctggca acctcccgga acaactcacc gtcacatccc
      121 tattgacggg ccacgccata cccgtgcttc ccgttcctgc tcttcatgcc aggaccgcgc
      181 acgctcccgt tccaggcgtg cctgcctttc ctgttcatcc cttatctgct gttcgtgata
      241 aataaccgac tcaagtggtc cacctgcccg gctaatctct gcacctgctg actcaagtcg
      301 tcgcactgtt ccctcagttg cccgttctcc tgtcgtgtca gctcgaacat atgctgcaaa
      361 tccgtgaagg cgctctccca gtctttcagc cgctgcgtat agtcctgctg caattgctct
      421 aaggcgttca gtaagtgcat ttccagctct gtcatactca cttactccct gaccagtctt
      481 actgcgttct tcttctccac cgtccagttg ttttccccct tcaccccgga cggcaacacc
      541 agaaatttcc cgttcctgcc ctcgtgatac gtcacacccc atgttttttc ccggagtttc
      601 gccagcgtct cttcctggtc cctgatagcc aggatgttcg ccgcaatccg gctttcctgc
      661 cactgaatca gcccccatga cgccagaaaa atcccgcccg tgacgcaaag cgccgtcagc
      721 gacgggtaca gtatccgccc tttgaccagc ttccagagca gctcttcctg ccgccgggcc
      781 agttcgttct ctgtggcgct gaactgcgcg ttcacggcac tgttcagcgt ctccagttgt
      841 tctttcaccg ctgctgtgtg tgcgctgata gcgtctctga ttttctgccc gtttaagttc
      901 agttccctgt ctacagacgc ttcgagcttc ctgaactcgc tgttcagcat gttctctgta
      961 gagacggcac gctctttcag tttcttctcg aagtctgtcc ccatttgtaa aagattgctc
     1021 atacagcgcc cctttcagcc tgagattacg cccaccctcc gggtcggcga tactgatact
     1081 gctcctggtt gtcctcacaa cctcaaaacc tgccgctgta agcgcctcag tgacatcctg
     1141 acgcgttttt agcgctccgg catggtaaag agcctccagt cccctcgtaa tcgcttctgc
     1201 ggcctcctgt ttcgctttcg gcagattatt cggggtgaca agtgtccgcc tgttctccgg
     1261 tgcgttcggg tcgtgcagcc cgtaatggtg attcaccagt gtctgccagg cattgattcg
     1321 cggacggtcc gctcggtcgt aatagggctg gagccgtttt ccgctcgcca gctccatatt
     1381 cgggatgaca aaattcagct caagacgccc cttgtcctgg tgctccaccc acaggatgct
     1441 gtactgattt ttttcaagac cgggcatcag tacccgctca aagctctcca tcaccctttc
     1501 acgctctccc ggtggcaggg tctgctctgc aaaagacaga acccccgagg tgtatttttt
     1561 cgcaaacggc gtggcatcga tgagttcccg cacctcttcg ggagcacccc gcagaactct
     1621 cgccccttcc cggttacgct cccggcccag caggtaatca accggaccac tgccaccgcc
     1681 ttttcccctg gcatgaaact taactatcat cccgttctcc ctgtttacgg acctcatccc
     1741 tcagctcact cagttcacgt ccgatggcca tcagtgcagc caccacatga acccggtcat
     1801 gccccgacca ctgtccgctg tttatcttcc gggctatctg attcaggtta ttgccgaccg
     1861 aagcgaactg gcgcaacagc ggcggtgcca gtgtcggaag acctgacgtt ttcgatggcg
     1921 gtgcccccag gcagacctta cgcatccatg acgcaagttg tgttccctca caacgtgcca
     1981 gcagccgcgc atgttcctca tccgtgaccc gtatcgtgag catcctttcg cgtttcaccg
     2041 gtatcattaa aaacctccga cagactcccc acacatggag aaacagaact gtgactaaac
     2101 aggaaaaaac cgcccttaac atggcccgct tcatcagaag ccagacgctg accctgctgg
     2161 aaaaactgaa tgaactggac gccgacgacc aggctgacat ctgcgaagcg cttcacgacc
     2221 acgctgacga gctttaccgc agctgcctcg cacgcttcgg agatgacggt gaaaacctct
     2281 gacacatgca gctcccggag acggtcacag ctggcctgtg agcggatgcc gggagcagac
     2341 aagcccgtca gggcgcgtca gcgggtttta gcgggtgtcg gggcgcagcc atgacccagt
     2401 cacgtagcga tagcggagtg tatactggct taatcatttg gcatcagtga ggattgtatg
     2461 aaaagtgcac catgccgggt gtgaaatgcc gcacagatgc gtaaggagaa aatgctcgtc
     2521 caggcgcttt tccgcttcct cgctcactga ctcgctccgc tcggtcgttc ggctgcggcg
     2581 agcggtatca gctcactcaa agacggtaat gcggttatcc acagaatcag gggataacac
     2641 caaaagaaac atgtgagcaa aaaacaagaa cccggaaaag gccacgcagc tggcgttttt
     2701 ccataggctc cgcccccctg acgagcatca caaaaaccga cgctcaagtc agaggtggcg
     2761 aaacccgaca ggacttaaag ataccaggcg tttccccctg gtggctccct cgtgcgctct
     2821 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttt gggaagcgtg
     2881 gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2941 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3001 cgtcttgagt ccaacccggt aagacacgac ttaacgccac tggcagcagc cattggtaac
     3061 tggatagtgg atttagatac gcagaactct tgaagttgaa gccttatagc ggctacactg
     3121 gaaggacagc atttggtatc tgtgctccac taaagccagt tacccggtta agcagtcccc
     3181 aactgactta accttcgact aaaccgcctc cccaggcggt tttttcgttt acaggcagca
     3241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgcg
     3301 atccccgtca gttcagaaga cgaagatggt gcaacggttc ctccttgtac atcacgaata
     3361 tagtttgctt gacatcctga caagaataat attgtaatta gaaaaattat tattttcata
     3421 tttctttcca acaaaagtaa aaatacttat gcatttaaaa tactacctac ataatttacc
     3481 tgaatcactt ataccatgga ttcttatttt aatatttaac gacaatgata acactccttt
     3541 gttatttata tttatatcat caatacatgt attgctatat ccatactcta aattaaccat
     3601 atctagatat atcaaagaaa atacaaagtt aaaaaaagaa ccctggtact tatgcaagtt
     3661 atctgcattg ttttatttat taatggcaat cccagtagga ttgccaagtt tcatatatta
     3721 cactctaaag agaaattaaa tccctaacaa ctcattaagt ttgctcaact catcatcccc
     3781 tatgtaagaa gaaacaatac ctgtgacaat tgcaatcccc cagaaaccaa gaggcacacc
     3841 aacaataaaa ctaaacatta atgccacaat ttttgccaca cctacatcga ccgcactttt
     3901 ctccagcgtg ataaacaaag gtcgccagtt accagtttct attgcatttt taaaatcaga
     3961 accaacatca tatagaaaag atactcgact ggtgatttta agcgactttg atatcttcgt
     4021 taaattctta gataactcat cataattaac agactctaac gcattaaaaa tagcatcccg
     4081 atcaacctta ctgaatttct tatccagtac attcttatac ttttcaaatg ctgccagagc
     4141 ttcatcaaca ccttgaattt tctttccttt agacttttcc gctaaatcct gagcaatttt
     4201 tgcgtatttt tctccatatt gttcggttat atattgataa aatccaacca tagtatcaac
     4261 agcatctttt atctggctct tttcaagagc atcctgagct tcttttaatt tctgctctgc
     4321 ctctttaacc tcagcggcct taccatccct gaaactaaca gcattcttaa gttcattttc
     4381 aactgcggag tactcagcct gcttagcttc aagctgacgc tgtagttttt tctgaacgtc
     4441 acgccatccc ggaaatccgg tgaccttcac gtcaacactc ttctgagcgt tttcaagctc
     4501 tcctgcaact ctggaaacag tagcttgctt actctgtaca tcactttcag ccttcgccag
     4561 ttcagctttc gcctcagcta aacgcttctc tgcttccgcc accgcctgtt tctcttcatt
     4621 aagagtttca tgttcagcga cctctgcatt ttcacgagcc ttttcttcag cttccttctg
     4681 tttattcaca tcaccaattt tactttgcaa tgtattttta taagaattaa gtttgctaat
     4741 atctgcatca agctgatttg attttttttg caactcatca acatccctct caagatcagt
     4801 gataccatga taagaatgat gcttgaatac ttttttcatc tcctcgattt tcttctgttt
     4861 ttcactaatc tgtgtagcaa ttttattctt ctgcttttgt ttttcattaa ttacattacc
     4921 caccaccttc gaactcttat ccatatcact gacctgagca ttcgttggtg cagtattaac
     4981 tgaagcagtg ttattgttgt tatttcctga gctttctgca aaaagtgatg gcatatcact
     5041 aattaaagaa ttaagaactc tggagacccc tccaaatgga ttatcaacca gagttgaatt
     5101 atcttctgtc ataacaatac cattcatcac cggaaggcca tcattattaa tgacaacatc
     5161 accccacgga gtcagatatg actcaccggt tttcatcaca gttgatgtag aaccagatga
     5221 atttgaattt ccctgaccac cattattacc atgcccagag ccaccacccc agtgaacccc
     5281 actattacta ttattatttc caccttgctg attcagattc gcccctgttc cccccataga
     5341 ctcaccagca gttggtccat atccacttag ttctttagcc ataaattcct ctttgataat
     5401 taaaacaata aattaaaaac aatatactgt acatataacc actggtttta tgtacagtaa
     5461 aaacctacta ctcagcattg tccatgtcaa gagcatggat tttcattttt gcaataagga
     5521 tcacactatg gggaggcagg cattgagaac gtcgaaacag aacaccggag caaatcagga
     5581 tgagatataa aactgttgga tcatgaaaaa acggagaacg atgtgagcaa atcaccccgc
     5641 cataaactga acaaaacaga caaacgactt ctcgacaccc ttgttgctgc cggatatgag
     5701 catgacaaag cccgtgacct catccagaaa caggtttaca cgctgacact ggctgatcag
     5761 cgtcatgtgg tcagtgaaat cagtaatggt gtgaatccca cccaggctta ctcggcggta
     5821 taccaggcaa gacgcattcg cctcgcccgt aaatatctga acggtaaaaa ggttatggaa
     5881 gaaaccgggg aaaatacgcc cccatcagcg taaggatttc ttttgccgct ccagagactc
     5941 cagtttttta cgcaaatcct ctcttttttg ggcatctctg gtgccagcca gctctgctct
     6001 caactcatcg atctgaagtt gtatcttcag tctgttactg aacattttct gtctggcatt
     6061 aacatccgca acaatgccgt tttttgtctt ctcggccttt tgttgaaaaa cattgctgtc
     6121 cgcatgactg gcaaccgaag cagaaagaac actaaaaagc aggactggca cacatttttt
     6181 cacgggatta ttcctgactc attgaccatc aaatcacatt gggagtaaac cgacgtatga
     6241 taagagatac tcttcggaga tataactccc tgagtatcaa gattaaaaac gcaaggagat
     6301 gtttatgaga tctgccgctg ccaggctgct tctgatacct ctgataacag caacaatagc
     6361 tcttacagga tgcacaccaa agaccagcct ggaacgacat acccggcatt atgtttatgc
     6421 ttcagatgat ggatttgatc ctaacttcta cacccagaaa gcagacacca tacgtatgat
     6481 gctcccgttc tttcagcagt tccgggatat ggggatgaaa gacaaagcag ccggagtatc
     6541 agcagaaacg gcacagcaac gtgtaaaaga attccactca gaaaaatttt ttcactcact
     6601 ccggagcaca acagcctttg ctggcagaaa atacacaaac agcgatatgc cttcgccgaa
     6661 aaaaatgaaa ctaatggcag acaccatttc tgcggtttat ctcgatggat acgagggcag
     6721 acagtaaggg atttaccata atcccttatt gtacgcaccg ctgaaatgcg ttcagcgcga
     6781 tcacggctgc tgacaggtaa aaatggcaac aaaccacccg aaaagctgcc gcgatcgcac
     6841 ctgataaatt ttaaccgtat gcatagctat tcagccatgt gaataacgct ggttttgcct
     6901 gcgtaaacct catgacactg ttttttttcc atcttttcag ttgatgacat acgcagacat
     6961 cgcgggatga ggctgaggaa tgagcgcg
//
