LOCUS       CP054451                6969 bp    DNA     circular BCT 14-JUN-2020
DEFINITION  Escherichia coli strain SCU-488 plasmid pSCU-488-2, complete
            sequence.
ACCESSION   CP054451
VERSION     CP054451.1
DBLINK      BioProject: PRJNA624897
            BioSample: SAMN14943542
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Hartman,A., Gonzalez,A.,
            Gill,M., Pandori,M. and Hess,D.
  TITLE     Title: F plasmids are the major carriers of antibiotic resistance
            genes in human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Hartman,A., Gonzalez,A.,
            Gill,M., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 44x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/04/2020 19:12:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 4,995
            Genes (coding)                    :: 3,821
            CDSs (with protein)               :: 3,821
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 1,174
            CDSs (without protein)            :: 1,174
            Pseudo Genes (ambiguous residues) :: 0 of 1,174
            Pseudo Genes (frameshifted)       :: 1,057 of 1,174
            Pseudo Genes (incomplete)         :: 111 of 1,174
            Pseudo Genes (internal stop)      :: 69 of 1,174
            Pseudo Genes (multiple problems)  :: 60 of 1,174
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-488"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-488-2"
                     /geo_loc_name="USA: California"
                     /collection_date="2018"
     gene            complement(1..471)
                     /locus_tag="HPE47_25475"
                     /pseudo=""
     CDS             complement(1..471)
                     /locus_tag="HPE47_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     gene            complement(449..991)
                     /locus_tag="HPE47_25480"
                     /pseudo=""
     CDS             complement(449..991)
                     /locus_tag="HPE47_25480"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1148..1470)
                     /locus_tag="HPE47_25485"
                     /pseudo=""
     CDS             complement(1148..1470)
                     /locus_tag="HPE47_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1535..1726
                     /locus_tag="HPE47_25490"
     CDS             1535..1726
                     /locus_tag="HPE47_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QKP94432.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2751..2888)
                     /locus_tag="HPE47_25495"
     CDS             complement(2751..2888)
                     /locus_tag="HPE47_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QKP94433.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            2936..3277
                     /locus_tag="HPE47_25500"
     CDS             2936..3277
                     /locus_tag="HPE47_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="QKP94434.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin transporter"
     gene            complement(3274..4839)
                     /locus_tag="HPE47_25505"
     CDS             complement(3274..4839)
                     /locus_tag="HPE47_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="QKP94435.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDTTVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-10"
     gene            5099..5536
                     /locus_tag="HPE47_25510"
     CDS             5099..5536
                     /locus_tag="HPE47_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QKP94436.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     gene            5617..6036
                     /gene="exc2"
                     /locus_tag="HPE47_25515"
     CDS             5617..6036
                     /gene="exc2"
                     /locus_tag="HPE47_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QKP94437.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(6598..6831)
                     /gene="mbeD"
                     /locus_tag="HPE47_25520"
     CDS             complement(6598..6831)
                     /gene="mbeD"
                     /locus_tag="HPE47_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QKP94438.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     Typing          complement(1812..1899)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          1910..2468
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.783"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1072)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.277"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1152..1375)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="2.01e-53"
     Typing          complement(1373..1503)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-53"
     Typing          complement(6495..6967)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.636"
                     /coverage="100.0"
                     /evalue="8.31e-53"
ORIGIN
        1 tgctgatttc ccgtacggaa ggaccagaaa tttcctgttc ccgtcctgta cgaactccac
       61 gccccatgtc ttcgcgttca gtttctgcaa tgtctcttcc tgcttcctga tatcttccag
      121 gttcgcctgt atcatccctc caagatacca gagcgtcccg caactcacgg taaacaggaa
      181 aatgaccatc cccagtaaca tcatgcccgt attccctgcc agctttaaca cgtccttcct
      241 gtgctgcatc atcgcctctt tcaccccttc ccggtgcttt ttcagtgatt cctctgtcga
      301 agctgtgaac agggctatag cgtctttgat tttcgtctcg tttgatgtca cagccttgct
      361 tacagatttt tcgagcttgc tgaactcgtc gttcagcatt gtctctgtag attcggctct
      421 ctctttcagc tttttctcga actccgcgcc cgtctgtaaa agattgctca tagaatgctc
      481 ctttcagtct gatattcttc ccgccgttcg ggtctgcaat gctgatactg cttcgcgtca
      541 ccctgaccac ttcaatccct gcttccgtga gcgcctgaat cacatcctga cggcctttta
      601 tctctcctgc atggtaaaga gcatctatcc cgcgtgtgac gccctcagca aacgcctgtt
      661 tcgtttccgg caggttatca gggagagtca gcgtccggcg gttctccggg gcgttcgggt
      721 catgcagccc gtaatggtga ttcaccagcg tctgccaggc atcaattctc ggcctgtctg
      781 cccggtcgta gtacggctgt aggcgttttc cggtctccag ctccatgttc ggaatgacaa
      841 aattcagctc aagccgtccc ttgtcctggt gctccaccca caggatgctg tactgatttt
      901 tttcgagacc gggcatcagt acacgctcaa agctcgccat cactttttca cgtcctcccg
      961 gcggcagctc cttctccgcg aacgacagaa caccggacgt gtatttcttc gcaaatggcg
     1021 tggcatcgat gagttcccgg acttcttccg gattaccctg aagcaccgtt gcccttcccg
     1081 gttacgctcc cttcccagca ggtaatcaac cggaccactg ccaccacctt ttcccctggc
     1141 atgaaactta actatcatcc cgcgccccct gttccctgac ggccagacgc aaccggcgca
     1201 gctcatcccc gatggccatc agtgcggcca ccacctgaac ccggtcgccg gaagaccact
     1261 gtccgctgtt caccttacgg gctgtctggt tcaggttatt cccgatggct gccagctgac
     1321 gcagtaacgg cggtgccagt gtcggcagcc taccggaacg ggcaaccggc tacccaggca
     1381 gacccgccgc atccataccg ccagttgttt accctcacag cgttccagta accgggcatg
     1441 ttcatcatca gtaacccgta tcgtgagcat cctctcgcgt ttcatcggta tcattacccc
     1501 catgaacaga aatccccctt acacggaggc atcagtgact aaacaggaaa aaaccgccct
     1561 taacatggcc cgctttatca gaagccagac attaacgctg ctggagaagc tcaacgaact
     1621 ggacgcggat gaacaggcag atatctgtga atcgcttcac gaccacgccg atgagcttta
     1681 ccgcagctgc ctcgcgcgct tcggggatga cagtgaaaac ctctgacata tgcagctccc
     1741 ggagacggtc acagcttgcc tgtgagcgga tgccgggagc agacaagccc gccagggcgc
     1801 gtcagggtgt tttagcgggt ttcggggcgc agccctgaac cagtcacgta gcgatagcgg
     1861 agtgtatact ggcttaacta tgcagcatca gtgcggattg tatgaaaagt gcaccatgac
     1921 cagtgtgaaa taccgcacag atgcgtaagg agaaaatgca cgttccggcg cttttccgct
     1981 tcctcgctca ctgactcgct acgctcggtc gttcggctgc ggcgagcggt actgactcac
     2041 tcaaaaacga taatacagtt atccacagaa tccggggata agaccggaaa gaacatgtgc
     2101 gcaaaaaggc aaagcccaac aaaaggccac gccgatggcg tttttccata ggctccgccc
     2161 ccctgacgag catcacaaaa accgacgctc aagtcagagg tggcgaaacc cgacaggact
     2221 ataaagatac caggcgtttc cccccggaag ctccctcgtg cgctctcctg ttccgaccct
     2281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2341 ctcacgctgt tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2461 cccggtaaga cacgacttag cgccactggc agcagccgtt ggtaacttgg ttagcagagc
     2521 gatgatggcg caaatggtgc aacagagttc ttgaagtagt ggcccgacta cggctacact
     2581 ggaaggacag tatttggtat ctgcgctctg ctgaagccag ttacctcggt taagcagttc
     2641 cccaactgac ttaaccttcg atcaaaccgc ctccccaggc ggttttttcg tttacaggca
     2701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaaca
     2761 gagatccccg tcaatttaga agaagatgat ggagccacag tccctccctg aacatcacgt
     2821 atatagttag cctgacatcc aacaaggagg tttatagcga atattcccac aaaaaatctt
     2881 ttcctcataa ctcgatcctt ataaaatgaa aagaatatat aacgaggttt aatttatgag
     2941 tttaagatac tacataaaaa atattttgtt tggcctatac tgcgcactta tatatatata
     3001 ccttataaca aaaaacaacg aagggtatta tttcctagcg tcagataaga tgctatacgc
     3061 aatagtgata agcactattc tatgcccata ttcaaaatat gctattgaac acatattttt
     3121 taagttcata aagaaagatt ttttcagaaa aagaaaaaac ctaaataatg cccccgtggc
     3181 aaaattaaac ctatttatgc tatacaatct actttgtttg gtcctagcaa tcccatttgg
     3241 attgctagga ctgtttatat caataaagaa taattaaata cccaacacct catttatagt
     3301 attaagttta ttcttatcaa taaaggcaca tagaatgcct gtaacaatag caatccccca
     3361 gatacctaat gtagttccag caagcacact aaaaagtaaa acaacaacat aactaactcc
     3421 agcatctaca gctttcttct ctaatgtaag aaatagcggc ttccagtcac ctgtatcctt
     3481 aatttttagg atatcagata ccacatcata tccaaaagaa acatgtcccg taatcttcaa
     3541 gtacttggca aactgatcta aatgcttagc ccagtcttca tacttaaccg cctccagtgc
     3601 attgaaaatt gcatcacggt ctgctttgct gaatttctta tttaatacat ccttgtattt
     3661 ctcaaaagca gctagagctt cattcacatt gctgattttc ttacctttag atttatcagc
     3721 aagttcctgt gccatttttg aatatttttc accatatttt tcagatagcg tttgataaaa
     3781 gctaactgtt gtatccacag catccttaat ctgcgagttc aggagattag tctgtgctgt
     3841 tttcaaatta tcttcagcat catgaatacg tgaaactgct gtacttcgct tattattagc
     3901 ctgagaaata gcctcttgga ttccatttat ctcagagcta atctggttaa gacgcgtttc
     3961 tgatgctgtt acctgctttt gtttttcctc catctcatca ccagctctcg cccgtagtct
     4021 ggtggcctca aaaaaaggac gactctgaag cggatcattc gccctaggag ataaaagttt
     4081 aaccctttca tccagttctt tatatttagc agatgcctga tccaactcat ttcgttttcc
     4141 ggacagcgta ttcgtttctg catcacgggc atgaatactg gagcttaaac gggtattgag
     4201 cgtattaatc tcttcatcca ctttcgccag ctcagattgt gcagcagcaa gatttttttg
     4261 tgccacctcc acagcccggg cctcttcact caatgctgcc aggcgtttct cttcatcctc
     4321 agccagtttc aattggcgtt ctgtttcagc ctgctccttc tctatctctt tacgtcgttg
     4381 ttctgcttcc tgaaaagcct tttccgcagc ttccgcttct ttacgggctt tttcttctgc
     4441 ttttgcaagg cgcaaccgct ctgcttctgc ctgcatcgct gcattattgg catgagccag
     4501 gtcaataacc tccggatgag tagaattatg gcgaagcgcc tcattcacaa tatccttcag
     4561 atgttgagtc agcgcatccc ggtttgcttt tgctttagcc tgtgcttctg cggcagcttt
     4621 tgctcgggca gcctgttctg cctgcgtttt cttcaattga gcagtagacc atttagctgt
     4681 ggcatgaatg gctgcagaac tttcactttt gctacctcca gttccaccac caccgccaga
     4741 gccactcccg tctggattac cattcaaaag agtaatgatt acctgcccct tatcatcata
     4801 aggaacacca tctttatagt acgctacagc tgtttccatt ataaaatcct ctttgacatt
     4861 aaaaacaaat agttaaaagc aatatactgt atatataatc actggtttta tatacagttt
     4921 aaaattacgc cgctgcattt tccctgtcaa ccctgtggat tttcattttt gtgaaaacga
     4981 tcaaaaaaac agccacccac accacgtgct atttccaccc gatgccacaa aaaccagcac
     5041 aaacattacc gttctcagac ctcattatgt tttactgaaa ccatgagatg agacacctat
     5101 gggacactgt aactttatgg catggcacac actccgggac gcactgaaaa tgactggaag
     5161 atcgcgttca cagttttacc gtgatatgcg ctcaggtctt gtcagttacc gtaccagcca
     5221 ggacggacga cgggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagcg
     5281 gaatgagaca ccagaaaggc acactcaagg acactcagaa aaacctcatg atcagcagac
     5341 agaacgcatt ctccgggaac tgaatgagct gaaacaatgc ctgacgctga tgcttgagga
     5401 taaacaagcc caggatacgg agcgccggcg ccaggaagca gaacggaaac agctacagaa
     5461 tgagatagcc cagctcaggc aggctctgga gcaggaaaag aaacggggat tctggtccag
     5521 gctgttcggt cactgaacgc gctcagaaac tgatgataag ataatcttcg gataataact
     5581 caccgagaat aaatacttta aggtagggag acactcatga gacgtaccgg aaacaaactt
     5641 tgtcttatcg ccatgataac agcaacagta gctctcacag cctgtacccc aaagggcagc
     5701 gtggaacaac atacccggca ttacgtatat gcttctgatg acggttttga tcccaacttt
     5761 tccacccaaa aagccgacac aacacgaatg atggtgcctt tttttcggca gttctgggat
     5821 atgggagcta aagataaagc gacaggaaaa tcacggagtg atgtgcaaca acgcattcaa
     5881 cagtttcaca gccaggaatt tttaaactca ctccggggca caactcaatt tgcgggtact
     5941 gattaccgca gcaaagacct taccccgaaa aaatccaggc tgctggctga cacgatttct
     6001 gcggtttatc tcgatggcta cgagggcaga cagtaagtgg atttaccata atcccttatt
     6061 gtacgcaccg ccgaaacgcg ttcggtgcga tcacggcagc agacaggtaa aaatggcaac
     6121 aaaccacccg gaaaactgcc gcgatcgcac ccggtaaatt ttaaccacat gcatacctat
     6181 gcagccaggt gaatcacgca ggaattgcca gtcagagacc gctgaaacgg atttttttca
     6241 tactttcgga agcgaagaag acggggattt gagcaggaaa acagaagttc aggggaatga
     6301 gcgcgatctg gcaatagagg caaaaacacg actatgaaag acacaccaga atcgcgcccg
     6361 tatgcgtttt aacgcgttcc tgtgttttca gcaaggttta tgactgaagc gcgttcccgg
     6421 tgccccgaac caaccattat cccaaaaaat gcaccggaaa cgcgctgaca acattttagc
     6481 ctgtctctga ttaccatccc agcgaagggc catccagtgt ccgttcctgt atttccagct
     6541 ggcgctcacg ctccagggat aactcatgtt cgcgttcctg tatcagccgt tcgtccctta
     6601 tctcctgttc tcgctgtata actggctcaa gcgttctgtc cgctcgctca agtgctgcac
     6661 ctgctgactc aacttcatga cccgctcgtt cagcatcgcg ttgtcccgtt gcgtaagcga
     6721 aaacattttc tgcaattcca cgaaggcgct ctcccattcg ttcagccgct gcatatagtc
     6781 ctgttgcagt tgctctaatg cgttcagcaa atgtctttcc agctccgtca ctctgtgtca
     6841 ctccatcagt tgtacccagt ccttcccctg atggggaatc acctctgctg atttcccgta
     6901 cggaaggacc agaaatttcc tgttcccgtc ctgtacgaac tccacgcccc atgtcttcgc
     6961 gttcagttt
//
