LOCUS       CP054330                9138 bp    DNA     circular BCT 10-JUN-2020
DEFINITION  Escherichia coli strain SCU-121 plasmid pSCU-121-2, complete
            sequence.
ACCESSION   CP054330
VERSION     CP054330.1
DBLINK      BioProject: PRJNA624897
            BioSample: SAMN14609781
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the major carriers of antibiotic resistance genes in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/26/2020 18:38:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,901
            CDSs (total)                      :: 4,791
            Genes (coding)                    :: 3,570
            CDSs (with protein)               :: 3,570
            Genes (RNA)                       :: 110
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 1,221
            CDSs (without protein)            :: 1,221
            Pseudo Genes (ambiguous residues) :: 0 of 1,221
            Pseudo Genes (frameshifted)       :: 1,095 of 1,221
            Pseudo Genes (incomplete)         :: 102 of 1,221
            Pseudo Genes (internal stop)      :: 95 of 1,221
            Pseudo Genes (multiple problems)  :: 67 of 1,221
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-121"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-121-2"
                     /geo_loc_name="USA: California"
                     /collection_date="2016"
     gene            1013..1897
                     /locus_tag="HHJ34_24370"
     CDS             1013..1897
                     /locus_tag="HHJ34_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43398.1"
                     /translation="MNVLSQLINDALNHQGGHVRTDGNGDITQFIPMEPAAVRYLSMND
                     VLPTKGGKQTTFNAVLAERSLVAQAGAEIIRIPGPMTTQPTGQTGATTGREVADRFVVI
                     RPGAFAKVADGEEISMSGKPYMVAAFDHNTAPAYGVGYTLTRQQLKHEFADDTVLEAVN
                     TAIERGIADLADFVLLNHLETAAETLASPSFTAVAQKIAAKNLRFDEVKAIVGGDCTGL
                     ELQDGVLRAYGVRAEISGQTSSTIIGAFGNAAVALDDEIRVTARRVLNGAVEVVVWVNA
                     SALVPDSTMFWKA"
                     /name="hypothetical protein"
     gene            1900..2214
                     /locus_tag="HHJ34_24375"
     CDS             1900..2214
                     /locus_tag="HHJ34_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43399.1"
                     /translation="MRWGSKKAAAERAALPGEILAAIEGVAMYVRHGDENTPRIVVQPV
                     GWSGFIYSDDAAEKWIRKAYPELKQYQIERAVNYLASLVRSHYREQRPKQKRESWMNRY
                     "
                     /name="hypothetical protein"
     gene            2218..2382
                     /locus_tag="HHJ34_24380"
     CDS             2218..2382
                     /locus_tag="HHJ34_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43400.1"
                     /translation="MKRQETRQHVTVRLAPDLVRQVEREQAKLERRTGIRPSRSQVIEK
                     FLSSGLNGK"
                     /name="hypothetical protein"
     gene            2597..2914
                     /locus_tag="HHJ34_24385"
     CDS             2597..2914
                     /locus_tag="HHJ34_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43408.1"
                     /translation="MKNHDLYESMKEEARLILSETKRFQTISEKNPISYEAADLLASLR
                     NRLNAFEHMTDEAGRAMRNEAESHASTLERKKWQPSSRISSGERAKRQAVKRGAILVRS
                     A"
                     /name="hypothetical protein"
     gene            2917..3126
                     /locus_tag="HHJ34_24390"
     CDS             2917..3126
                     /locus_tag="HHJ34_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43401.1"
                     /translation="MATNNNIYEAVLNVIGVADKMTKNNASQLTDAQKLAIKNLSGAMK
                     SWKGITFGMRDITANDKPTESKTA"
                     /name="hypothetical protein"
     gene            3136..3342
                     /locus_tag="HHJ34_24395"
     CDS             3136..3342
                     /locus_tag="HHJ34_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43402.1"
                     /translation="MLDRIHTKPEGTNPWPELVDMVLPKVMVCETAEGMQAQRKHRTEC
                     ALQLAAAALRAAGEHTLAENLLN"
                     /name="hypothetical protein"
     gene            3364..5655
                     /locus_tag="HHJ34_24400"
     CDS             3364..5655
                     /locus_tag="HHJ34_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="QKN43403.1"
                     /translation="MANKKLNATITIGGAVSGSLRGAFGTVEKSTIKIGAAIKHMSREQ
                     RQLNDAMKRYGQNSTMVDRLRGRYENIVGQVERLRAAQERLNRVQRASTENLAKQAQLR
                     GQIIDTVMAGAAVAAPIKIAADREQHAIGIAKQLDGARDAAGNLTAKFWEMRKAVADLG
                     HEIPLATNDLFDMATAGLRMGVAGDQILGFTRNVAKLASALELNPEEVADNMGKIQNVY
                     RLTQAELVRLGDAINYLDDQNTVKGGELIDFLQRVGGSASLAKLTANDMAAIGTTLISM
                     GESADTAATSVKALVSKMTLGSKSTKSAREAFKELGYSAQEVAKSMQLDSVKTIENFMK
                     TVNKLPDYKKSGILVSIFGQEHVGSISKLAANMDKFGEALHQANGEMSKGSVQKEFQNT
                     VNTTNAQMVILRNRTADVADNIGTVLLPTVNDAAKGIGRITTVVANFAAEHPGLTKAVV
                     GTAMALTSLRVATLAAQFGFTFLKGGVLQMSSLFARMNASAVLASTRGLPAVATGIRAV
                     GAAFISTGVGAAVAGLALAGYQIYRHWAGVKAFFGGVGEGIKSGLEPLSDALHRLYERM
                     GPLKPVIDGVGAAVKTVFNWFTSLTEPVKYSGEELDKAGKMGMTFGKTLAAGIELVTAP
                     ITFLIDKILWVSDNIGNLTNKALEFKNAVSDVAGGAWQKTKDFFSNPFGDDESSQPAAT
                     VAPGASLPSPALANRTGNTTVNTNDQYTINVKAEPGMNEDALANKVAQKLKQQQGIRNR
                     SMMNDGLAAP"
                     /name="phage tail tape measure protein"
     gene            6058..6333
                     /locus_tag="HHJ34_24405"
     CDS             6058..6333
                     /locus_tag="HHJ34_24405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43404.1"
                     /translation="MKLHDLADAILKTRTVETRCDAAFKFADAMLRNGSSITDTAEATY
                     RIVMRGNKAYRLNKDDAWRITAHAYAEQLAEREVATVLAQIAGGAK"
                     /name="hypothetical protein"
     gene            6330..6563
                     /locus_tag="HHJ34_24410"
     CDS             6330..6563
                     /locus_tag="HHJ34_24410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43405.1"
                     /translation="MSIITLHRLMREPATSQTHAQCVAIMQELIKQAIQSGRPLQYSIY
                     LGHLAEHTRFPFNRVDAWQMALDMQRKAGGAA"
                     /name="hypothetical protein"
     gene            6560..8848
                     /locus_tag="HHJ34_24415"
     CDS             6560..8848
                     /locus_tag="HHJ34_24415"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017116.1,HMM:NF020290.1,HMM:NF024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="QKN43406.1"
                     /translation="MSLAHEKYAWTEQDAAEALTFIDPDCDHETWTRLAFAVKAEFGDA
                     GFDVWNDWSKQGQKYDSKEARQKWKSAKRHGGVTIGTLIDLSMQNGWKPGTRKELSIDE
                     IQARAAKREAEAKAEAERKRHDQAAAAARALEIWEASEPLEGNEHPYLQRKGVLSFGLN
                     VGRWKGYENALLVPIRNADGALTSLQAIFPNTNTDLGRDRDYLKDGQKHGCFHLIGGKP
                     VGEKPVIVVCEGYATGASIHQATGHYVAVAFDRSNLKAVAQAMRKMVGRATIIVAADND
                     QWEKGNPGKTDAGKAAQAVGGLVAVPEFDDLTTKPTDFNDLHKLQGIDAVRSQVQAVIP
                     APEEKRFLPLDSNVVPFDFPYTSDRMQPLNNWENLDWLLSQYGITARYNEISKEVEINV
                     PGKSYGGDGDDDSALIEVTSLCARNRMPKADVADYIKLIGKENRYNPVKDFILSRPWDG
                     CSRFADLLATIETPADYSRDLMALLMKRWLVSAVAATFEERGFWSKGILVFQGEQSLGK
                     TSWIKALLPEEEQEKLLLVGAAMDPSNKDSVSQAIGRWIVELGELDTTFRKSDISDLKN
                     FISRPHDVLRKPYDRRESKYKRRTVFFASVNPKHFLHDETGNVRFWTIPVTGLNYNHGI
                     DVQQLWAEVHHWYTKGERWWLEREEEQRLEAVNADHAQVDPLEEMILARFRWDETRMEV
                     YTEMSAAEVLIAIGYDKPTKSQATSCGNILRKLTGKEPRRTAKGRFYPIPPKELPKPQS
                     YYDETQGPF"
                     /name="toprim domain-containing protein"
     gene            8859..9086
                     /locus_tag="HHJ34_24420"
     CDS             8859..9086
                     /locus_tag="HHJ34_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QKN43407.1"
                     /translation="MSYRAIISTFDPENASTVNTELFQTFATAPEAEAHGRKSLAEIQQ
                     EFFQMGYAFIVSYFIVEVPAAEIIQFITRS"
                     /name="hypothetical protein"
ORIGIN
        1 gcttgtcagt gacacctagg tgtcattctg atttgaggtg tcatagttgt aagtatctgt
       61 ttttgcttat taatgacacc tatgacacct atgacaccta ttttgaaaga tttatcagac
      121 cgtaaaaacc gccgcgaaat aacggttacc ctttttttga aaataggtgt caaggtgtca
      181 ctgctaaccc atgcaatcag cacaaatcac atatgaatca tgtatttagg cgtgtgacac
      241 ctatagtgac acctcaaccc aaggtgtcat tctttcaggg catagctgcg caccgttatg
      301 gcgttatgaa tccactcatt cgtaaccacg ctttttcacg tctaaccaaa gcagcagccg
      361 gaataggtac aacatacaga agtatgatgt aactacagga aacgagcctt tcagccttgt
      421 tttgcgttca aacagtagct gtttagtaaa cagccagaac gttgcacata tcgcaacaat
      481 aagcctgtgt gaatacgggc tgaaagcgcg cggaatatgt cagattatgc gccgttcgcc
      541 tgccagcacc ttcctggtgc ttctttctgc ctgcaaactc cgcgatttaa cataatggac
      601 cttgcgcgca ccgcctggcc gcctgccagc ctcaaaaatc gaaggtactt ccgagggggg
      661 tacagcctgc gggggcattg gtcccgcgta attcgcgttg tgcgtgcccc tccaaaactc
      721 actttatgat tacgtcaagt aaatcaatca tttatgtgaa ccttatcaaa gcactgtcac
      781 catacgtgcg cgattgcaaa ataaagcgaa cacgttttac acacgctgaa ttgtaaagat
      841 gtgtaaatat cgattacgcc ggattatgcc gggttatttg caagtattca gggttataag
      901 ttactgattt taaattaaaa agctcacatt gtggctattg tgttcggtat ggttactgta
      961 tatctttaag tcattccaga cgatttattg cacaacaacc ggagcatttt taatgaacgt
     1021 tttatctcaa cttatcaacg acgcgttgaa ccaccaaggc ggccacgttc gcactgatgg
     1081 caacggtgat attactcaat ttatccctat ggaaccggca gcagttcgct acctgtccat
     1141 gaatgacgtg ctgcccacga aaggcggtaa gcagaccacc ttcaacgctg ttctggctga
     1201 acgttcgctg gtggcacagg caggcgctga aatcatccgc attcctggcc cgatgaccac
     1261 gcagccgact ggccagactg gtgcaacgac tggccgcgaa gtggctgacc gcttcgttgt
     1321 catccgccct ggtgcgttcg ccaaagtggc tgacggcgaa gaaatcagca tgagcggcaa
     1381 gccgtacatg gttgcagcat tcgaccacaa caccgcaccg gcttacggcg ttggctacac
     1441 cctgacccgc cagcaactga aacacgaatt cgctgacgat acagtgctgg aggcggtcaa
     1501 caccgccatt gaacgcggaa ttgctgacct ggctgatttc gtactgctga accacctgga
     1561 aaccgcagcg gaaacgctgg ccagcccgtc attcaccgca gtggcgcaga agattgcagc
     1621 caaaaacctg cgctttgacg aagtgaaagc catagttggc ggtgattgca ccggcctgga
     1681 actgcaagac ggcgttctgc gcgcctacgg tgtacgcgct gaaatcagcg gccagacttc
     1741 cagcacaatc attggcgctt tcggcaatgc agccgttgcc ctggatgatg aaattcgcgt
     1801 caccgccaga cgcgtgctga atggtgcggt tgaagtggtg gtttgggtga acgcatccgc
     1861 actggtgcct gactcaacta tgttctggaa ggcttgatca tgcggtgggg cagcaaaaaa
     1921 gcggcagcgg agcgcgctgc cctgccgggt gaaatcctgg cagctatcga aggtgtggcc
     1981 atgtatgtgc gccacggtga tgaaaacaca ccgcgcatcg tagtgcagcc ggttggctgg
     2041 tcaggattca tctacagcga tgacgctgct gaaaaatgga ttcgcaaggc ttacccggag
     2101 cttaagcagt accagattga acgcgccgtg aattacctcg cttccctggt gcgcagtcac
     2161 taccgcgagc agcgcccgaa acagaagcgc gaaagctgga tgaacaggta ttaagccatg
     2221 aaacgacaag aaacacgcca gcacgtaacg gtacgcctcg cccctgacct ggtgcgccag
     2281 gtcgaaagag agcaagccaa actggaacgc agaacaggca tccgcccttc acgcagccag
     2341 gtgattgaaa agtttctgag tagcgggctg aacggcaaat aatggccaag aatacaggtt
     2401 tcaccggtta cctgtgccca aaataaccgg ccaaatttaa ccttcatcgt tcctcagacg
     2461 atggatgctg gaaccaggtt ggtggtcatc ctggacggtc aaaaccacca ctaattccac
     2521 accggttgcg ccgcaccggt cacctaaaag cgcgcactca cttaatcatc aacaactatt
     2581 gttagcggcc aatactatga aaaaccatga cctgtacgaa tcaatgaaag aagaagcgcg
     2641 tttgatcctt agcgaaacaa agcgtttcca aaccatttca gagaaaaacc caatcagcta
     2701 cgaagcagcc gacctgctgg ccagccttcg caaccgttta aacgcattcg aacacatgac
     2761 cgatgaagcc ggtcgcgcca tgcgcaacga agcggaaagc cacgcatcga ccctggaacg
     2821 caagaagtgg caaccatcca gccgcatcag ttccggtgaa cgggccaagc gccaggcagt
     2881 gaagcgcggc gctatcctgg tgcggagcgc ataagaatgg caaccaacaa caacatttat
     2941 gaagccgttc tgaacgtcat cggcgtagcc gacaagatga cgaagaacaa cgcaagccaa
     3001 ctgaccgacg cacaaaagtt ggcgatcaag aaccttagcg gcgcgatgaa gtcctggaaa
     3061 ggtatcacgt ttgggatgcg cgacatcacc gccaacgaca aaccaacaga aagcaaaacc
     3121 gcataagggg gcaccatgct ggaccgtatt cacacaaaac cggaaggaac aaacccctgg
     3181 cctgaactgg tggatatggt cctgcccaaa gttatggttt gcgaaacagc ggagggaatg
     3241 caggcacagc gtaaacatcg caccgaatgc gccctgcaac tggctgctgc cgccctgcgg
     3301 gctgctggtg aacacaccct ggcagaaaat ctacttaatt aattctttca gagacaaatt
     3361 attatggcca ataagaagtt gaacgccaca atcaccatcg ggggtgccgt ttccggcagc
     3421 ctccgtggtg cttttggtac ggtggagaaa tccaccatca agattggcgc agcgattaag
     3481 cacatgtcgc gcgaacagcg ccagcttaac gacgctatga agcgttacgg gcaaaactcc
     3541 acgatggttg accgcctgcg cggtcgttac gaaaacatcg taggccaggt ggaacgcctg
     3601 cgggctgcgc aagagcgttt aaacagagtg caacgcgcca gcacggaaaa cctggccaag
     3661 caggcacagc tacgcgggca gattatcgat accgttatgg ctggtgctgc cgttgccgca
     3721 cctatcaaga ttgccgccga tcgtgaacag cacgccattg gcattgccaa acaactggac
     3781 ggcgcacgcg atgcagcggg caacctgacg gcgaagtttt gggaaatgcg caaggccgtt
     3841 gctgaccttg ggcacgaaat tccactggcc acaaacgacc tgtttgacat ggccacagca
     3901 gggctgcgca tgggcgtagc tggcgaccaa atcctggggt tcacccggaa cgtggccaaa
     3961 ctggccagcg ccctggaact gaacccggaa gaagtcgcag acaatatggg caaaatccag
     4021 aatgtttatc gacttactca agctgagctt gtgcgccttg gtgatgctat taattacctg
     4081 gacgaccaaa acaccgttaa aggcggtgag cttatcgact tcctacagcg tgttggtggt
     4141 tccgcctccc tcgccaaact cactgccaac gatatggcag caatcggcac cacgctgatt
     4201 tctatgggtg aaagcgcaga cactgctgca acgtcagtga aggcgcttgt ttccaagatg
     4261 acgctgggca gcaaatccac gaaatctgcg cgcgaagcgt tcaaggaatt gggttattcc
     4321 gcgcaggaag tggcgaaatc catgcaactg gatagcgtca agaccatcga aaacttcatg
     4381 aagacggtca acaagctgcc agattacaag aaatccggca tcctggtgtc catcttcggg
     4441 caggaacacg ttggttctat ctccaaactg gcagccaaca tggacaagtt tggcgaggcg
     4501 ttgcaccagg ctaatggcga aatgtccaaa ggcagtgtgc agaaggaatt ccagaacacc
     4561 gtcaacacca ccaacgcgca gatggttatc cttcgtaacc gcacagctga cgtggctgac
     4621 aatatcggca ccgtcctgct accgaccgtc aatgacgcag ccaaaggcat tggccgcatc
     4681 acaacagttg tggcgaactt cgcggcggaa caccctggcc tgaccaaagc cgtggtgggc
     4741 acagcgatgg cgctaacttc cctgcgagtg gccaccctgg cggcgcagtt cggcttcacg
     4801 ttcctgaaag gtggcgtgtt gcagatgtcc agcctgtttg cccgcatgaa cgcatctgcc
     4861 gttctggcca gcacccgtgg cctgcctgcg gtggctaccg gcattcgtgc cgttggtgcg
     4921 gcgttcatat ccactggcgt gggtgctgcc gtagctggcc tggcgctggc aggttatcag
     4981 atttaccgcc attgggccgg tgtgaaggct ttttttggtg gcgttggtga aggcatcaaa
     5041 tccgggctgg aaccgcttag cgatgccctg catcgcctgt atgagcgcat ggggccattg
     5101 aagcccgtca ttgatggcgt gggcgctgcg gttaagacgg ttttcaactg gttcacgtca
     5161 ttgaccgagc cggtcaagta cagtggcgag gaactggata aggccggtaa aatggggatg
     5221 acgttcggta agaccctggc ggctggcatc gagcttgtga ccgcaccgat cacgttcctg
     5281 attgataaga ttctgtgggt gtctgacaac atcggcaacc tgaccaacaa ggcactggag
     5341 tttaaaaacg ccgtgtctga tgttgctggt ggtgcgtggc agaaaacgaa agatttcttt
     5401 agcaatccgt tcggtgacga cgaaagcagc cagccagcag cgaccgtcgc ccctggtgcg
     5461 tccttgcctt caccggcact ggctaaccgc accggcaaca caacggtgaa cacgaacgac
     5521 cagtacacca tcaacgtgaa ggccgagcct ggcatgaatg aagatgcgct ggcaaataag
     5581 gtggcgcaga agctgaaaca gcagcaagga atccgaaatc gtagcatgat gaatgacgga
     5641 ttagcagcac cgtaatgaaa tgaaattgat gactgcaacc aaagccgggg tgaaaactct
     5701 ggcttttttt tatggttagc atttattaac cgattatgcc ggattatgtg ggattatgag
     5761 tgacatttac ctttttaaat gtctgaaatt atttatgaat tttcggccta tattttggca
     5821 aaatagcttg cagaaaccgg attgcagggt atcattcgaa ttgtggcgct tgcacaccgc
     5881 cacaaaaagg aaaacccccg gactgcgcga acaatccagg gggcttaatg aaagtctagg
     5941 ttagcggcct gactaggaaa aatactagca aagaccttta cctttagcaa gttaaacccc
     6001 ccttcattga gccaccgatt ttagcgcagc cctcaacgaa atattgagga atgcgaaatg
     6061 aaattgcacg acctggctga cgccatcctg aaaaccagaa ccgttgaaac tcgctgtgat
     6121 gctgcgttca aattcgctga cgctatgctg cgcaacggtt caagcatcac cgacacagcg
     6181 gaagcaacat accgcattgt aatgcgcggg aacaaagcct accgcctgaa caaagatgac
     6241 gcctggcgca tcactgcgca cgcctacgct gaacaactgg cggaacgcga agtggccacc
     6301 gtcctggcgc agattgccgg gggtgcgaaa tgagcatcat caccctgcat cgccttatgc
     6361 gcgaacctgc taccagccag acgcacgcac agtgcgtggc catcatgcag gaactgatta
     6421 agcaggccat ccagtctggc cgcccgctgc aatattccat ctacctgggg cacctggcag
     6481 agcacacccg cttcccgttc aaccgcgtgg acgcctggca aatggcgctg gatatgcagc
     6541 gcaaggcagg gggtgcggca tgagtctggc acacgaaaag tacgcctgga ctgaacagga
     6601 cgcagccgag gcgttgacat ttattgatcc tgattgcgac cacgaaacct ggacccgcct
     6661 ggcgtttgcg gtgaaggctg aatttggtga cgctggattt gatgtctgga acgattggag
     6721 caaacagggc cagaagtacg acagcaaaga agcacgccag aagtggaaaa gcgccaagcg
     6781 gcacggcgga gtaaccatcg gcacgctgat tgacctttcc atgcagaacg gctggaagcc
     6841 gggcaccagg aaagagcttt caatagacga gattcaggct cgcgccgcga agcgtgaggc
     6901 cgaagctaaa gccgaggcag aacgcaagcg ccacgaccag gcagcagcgg cagcccgcgc
     6961 cctggaaata tgggaagcat cggaaccgct ggaaggtaac gaacacccgt atttgcagcg
     7021 caaaggtgtg ctgtctttcg gcctgaacgt tggccgctgg aagggatacg aaaatgcact
     7081 gctggtgcca atccgcaacg ctgatggtgc cctaaccagc ttgcaggcaa tcttcccgaa
     7141 caccaacact gaccttggcc gtgaccgtga ctacctgaaa gacggccaga agcacggttg
     7201 tttccacctg attggcggta agcctgttgg cgaaaagccg gtgattgtgg tttgcgaggg
     7261 ttacgccact ggtgccagca tccaccaggc aacaggccat tacgtggccg tggctttcga
     7321 caggagcaac cttaaagctg tggcgcaggc catgcgcaag atggttggcc gcgccacaat
     7381 aattgtggcc gctgataacg accagtggga gaagggcaac cccggaaaaa ccgatgcagg
     7441 taaggcagcg caggcggttg gtggcctggt ggctgtgcct gaattcgatg acctgaccac
     7501 gaagccgacc gacttcaacg acctgcataa attgcaggga attgatgccg tgcgcagcca
     7561 ggtgcaggct gttattccag caccggagga aaagcggttc ctgccactgg attctaatgt
     7621 ggtgccgttt gacttcccct acacgtcaga ccggatgcag ccgcttaaca actgggaaaa
     7681 ccttgattgg ctgttaagtc agtacggcat taccgctcgt tacaacgaga tcagcaagga
     7741 agttgaaatc aacgtgcctg gcaaaagcta tggcggtgac ggtgatgacg atagcgcgct
     7801 gattgaagtg accagcctgt gtgcccgtaa ccggatgcca aaagctgacg tggctgacta
     7861 catcaaactg attggcaaag agaaccgtta caacccggtc aaagacttca ttctgtccag
     7921 accgtgggat ggttgcagcc ggtttgctga cctgttagcc accattgaaa cgccagcaga
     7981 ttacagccgt gacctgatgg cgctactgat gaaacgctgg ctggtgtcgg cagtggcggc
     8041 gacatttgag gaacgcgggt tctggtccaa aggcatcctg gtgttccagg gggagcaatc
     8101 ccttgggaaa acgtcatgga ttaaggcgct gctgccggag gaagaacagg aaaagctatt
     8161 gctggttggt gctgcgatgg accctagcaa caaagacagc gtatcgcagg caattggccg
     8221 ctggattgtg gagcttggcg aactggacac cacattccgc aagtctgaca tatcggacct
     8281 gaaaaacttc atcagccgcc cacatgacgt gctgcgcaag ccgtatgatc gccgtgaatc
     8341 gaagtataag cgcaggactg tgttcttcgc atcagtgaac ccgaaacact tcctgcatga
     8401 cgaaaccggc aacgtgcgtt tctggaccat tccggtgacc gggctgaact ataatcacgg
     8461 catcgacgtg cagcagttat gggccgaagt gcatcactgg tacacgaaag gcgaacgctg
     8521 gtggctggaa cgcgaggaag aacagcgcct ggaagcggtg aacgctgacc acgcgcaggt
     8581 tgacccgctc gaggaaatga ttttggcccg cttccgctgg gatgaaaccc gcatggaggt
     8641 ttacaccgaa atgtcagccg cagaagtgct gatagcgatt ggctacgaca agccaaccaa
     8701 atcgcaggcc acatcatgcg gcaacatcct gcgcaagctg actggcaagg agccgcgcag
     8761 aacagccaaa gggcggttct acccgatacc gccaaaagaa ttaccaaagc cacaaagcta
     8821 ttacgacgaa acccaaggcc cattttgagg aataaatcat gagttatcgc gcgatcatca
     8881 gtacatttga cccggaaaac gcatcaactg ttaacactga attattccag accttcgcaa
     8941 ccgcaccaga agcggaagca catggccgga aatcacttgc tgagattcag caggagtttt
     9001 tccagatggg ttatgcgttc atcgtcagct acttcatcgt tgaagtacca gcggcggaaa
     9061 tcatccaatt catcacccgt tcgtaacatc acaacaattt ttgttcaccg ccctggcaca
     9121 tgctggggct tttttatt
//
