LOCUS       CP053090                8719 bp    DNA     circular BCT 09-FEB-2021
DEFINITION  Staphylococcus epidermidis strain HD31-1 plasmid pHD31-1_2, complete
            sequence.
ACCESSION   CP053090
VERSION     CP053090.1
DBLINK      BioProject: PRJNA546129
            BioSample: SAMN14732896
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buttner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Distinct clonal lineages and within-host diversification shape
            invasive Staphylococcus epidermidis populations
  JOURNAL   PLoS Pathog 17 (2), e1009304 (2021)
   PUBMED   33544760
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buettner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 112x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2020 15:51:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,504
            Genes (coding)                    :: 2,428
            CDSs (with protein)               :: 2,428
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 43 of 76
            Pseudo Genes (incomplete)         :: 35 of 76
            Pseudo Genes (internal stop)      :: 25 of 76
            Pseudo Genes (multiple problems)  :: 21 of 76
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="HD31-1"
                     /isolation_source="nose; prosthetic joint infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pHD31-1_2"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2018"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(join(8429..8719,1..384))
                     /locus_tag="HJI00_12865"
     CDS             complement(join(8429..8719,1..384))
                     /locus_tag="HJI00_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="QRJ24126.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKGFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(805..1662)
                     /locus_tag="HJI00_12870"
     CDS             complement(805..1662)
                     /locus_tag="HJI00_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="QRJ24120.1"
                     /translation="MSGETVVYKNDMNLVPLRKFNNTEVNLFFTLCNKLKDKGNLTVNV
                     PFEELKELSNYYSHDKKLFIQDLEKFYDKVFSLTYREETENVIRKFILFTKVEIYKDKE
                     YVAIGVNPDLEHIINSLTSNFTKFELREMTHLKSTYSKHMFRILKQYKHTGYVKIKIDD
                     FRERLDIPNSYRMTNINQKVLAPIIKELGFIFNNLNINKIKAKKGRKIEWLEFTFDAEK
                     RIHSKRQPQMVNIGKSRQYISREKTPKWLEERTYEKQTQNEYDPQLEKEREAFLKQLQT
                     DWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(2331..2825)
                     /locus_tag="HJI00_12875"
     CDS             complement(2331..2825)
                     /locus_tag="HJI00_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QRJ24127.1"
                     /translation="MTQKRFISVAKHYIERINNDNLKQDFQTAIQEELKDVKTDTHKAI
                     EQLQTNQAELQQANNDYKATMDERIKHNETAVKQYDQAFHRLTKGITAMFFIIALVMVT
                     FLVLSPLGDWLGVQHFYEWLNYVLKTGHSAWRYFILIFYLVPYALFGGLIYAILSVYKR
                     I"
                     /name="mobilization protein"
     gene            complement(2839..3849)
                     /locus_tag="HJI00_12880"
     CDS             complement(2839..3849)
                     /locus_tag="HJI00_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="QRJ24121.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRE
                     LYGKTDGNQGHVIIQSFKPGEVTPEQCNQLGLALAEKLAPNHQVAVYTHADTDHVHNHI
                     VINSIDLETGKKFNNNKQALRNVRNFNDEVCIEHGLSVPEKDTARLRYTQTEKAIADPN
                     TKSTAQYSWKDEIREAIDQSQATNMNEFKDHLNQHGIEIERVTPKSITYRHLAEDKKVR
                     GRKLGEDYNKGGIEDGFERQIQRRRQQERDSDTECQPKRPTSSRDESTERDTGVTQSDW
                     DQFAQDTNELERSRQAAESARLADEKARRDREERERAKQASRRIINNDRENGLEL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3836..4045)
                     /locus_tag="HJI00_12885"
     CDS             complement(3836..4045)
                     /locus_tag="HJI00_12885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ24122.1"
                     /translation="MEQTKLRRLLFSIWILTVISLMILTLFFNFSFLKQWWFITICFLF
                     CAFGGLLIDHFIPKQKKRDTNGNN"
                     /name="hypothetical protein"
     gene            complement(4027..4410)
                     /gene="mobC"
                     /locus_tag="HJI00_12890"
     CDS             complement(4027..4410)
                     /gene="mobC"
                     /locus_tag="HJI00_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="QRJ24123.1"
                     /translation="MSEQNTFVASDETVGRNHKPNRKAPKQISFRVSESEYLKLQQSAE
                     TLNMSVPSFVKKKAQGARLVAPKLDQATRQSVAKDLSMLGANANQIAKYCNQHQHEAPN
                     YKALERNISELRERLDEVWNKLN"
                     /name="mobC"
     gene            4773..4973
                     /locus_tag="HJI00_12895"
                     /pseudo=""
     CDS             4773..4973
                     /locus_tag="HJI00_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494373.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5036..5710)
                     /locus_tag="HJI00_12900"
     CDS             complement(5036..5710)
                     /locus_tag="HJI00_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="QRJ24124.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKGFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            complement(5766..6665)
                     /locus_tag="HJI00_12905"
                     /pseudo=""
     CDS             complement(5766..6665)
                     /locus_tag="HJI00_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /name="ABC transporter ATP-binding protein"
     gene            6806..8098
                     /locus_tag="HJI00_12910"
     CDS             6806..8098
                     /locus_tag="HJI00_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="QRJ24125.1"
                     /translation="MKRSLIITFALLLTIVIIAPNSYASEYEPTPTEIARQYGYPNVTD
                     AYQPEGSINVSQTGQILYDYQSNQKWYPASMTKLMTMYLTLEAVNKGELSLNDKVHITD
                     KHYRMSTLPELSNTKLYAGETYTISELLQITVSNSSNAAALILADEVSGNVNDFTDLMN
                     KKAKALGMSNTHFVNPTGAENKQLKEFAPSKYKNQDNTTSTAKDFAILDHHVIKETPKI
                     LHFTKQLAPTQHGVTYYTFNHSLEGAKMSLLGTDGLKTGSSDVADYNHTITTKRNNFRI
                     NQVIMGAGDYVNFGGEKQRNMMGNAMMNRSFDQYSYKKVLSKGTHKINGKKYYVKDNLY
                     DVVPKGMNKKDYKFIVKDGSIHLDYNRQFLTKDDGPPKVEVTKPLLHKANTIAQTTWKE
                     HPVVTFVALALLAIAFILMVRSIIHLLFKRK"
                     /name="D-alanyl-D-alanine carboxypeptidase"
     Typing          4382..4596
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.3e-109"
     Typing          complement(806..1662)
                     /name="rep_cluster_1256"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2843..3810)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.427"
                     /coverage="96.0"
                     /evalue="1.58e-177"
ORIGIN
        1 ttgaggttta ccaaattgtt taatgagacg tttgataaac gcatatgctg aatgattatc
       61 tcgttgctta cgcaaccaaa tatctaatgt atgtccctct gcatcaatgg cacgatataa
      121 ataactccat tttcctttta ttttgatgta cgtctcatca atacgccatt tgtaataagc
      181 ttttttatgc tttttcttcc aaatttgata taaaattggg gcatattctt gaacccaacg
      241 gtagaccgtt gaatgatgaa cgtttacacc acgttccctt aatatttcag atatatcacg
      301 ataactcaat gcatatctta gatagtagcc aaccgctaca gtgataacat ccttgttaaa
      361 ttgtttatat ctgaaatagt tcatacagaa gactcctttt tgttaaaatt atactataaa
      421 ttcaactttg caacagaacc acataatgat taaaactgag aaatcaaact ctctactact
      481 aatttcatct aaatcaaaga aatttaattt aatccgtttc atcatatcat cctttctatt
      541 aataatttat aatagaaagg cacttccgtg cacaaaggag gtgtgcatat ggcgttaacg
      601 atactctcac ttgttggaac atttatctta tctgttattg ctggtgtagt tgcgaactac
      661 attacagata acctaaaaag ataaataaaa aaccccactt tgctttggtc ggcagtgggg
      721 ttttgacata tggcgaacca tacttcactt gttaccttca gtatacatca gcagatacta
      781 tttgtaaaaa ttcatctact tttattattc ttcccaatcg gtttggagct gttttaaaaa
      841 ggcttctcgt tctttctcta attgtggatc atactcattt tgtgtttgtt tttcatatgt
      901 gcgttcttct aaccattttg gtgttttctc acgactgata tattgtcgtg acttacctat
      961 attcaccatt tgtggctgtc gtttactgtg aatacgtttc tcagcatcaa aagtaaactc
     1021 taaccattca attttacgtc ctttttttgc ctttatttta ttgatattaa gattattaaa
     1081 aatgaagcct aattctttaa tgataggggc taatactttt tgattaatat ttgtcatacg
     1141 gtaactatta ggaatatcta gccgttctct aaaatcatca attttaattt ttacatagcc
     1201 agtatgttta tactgcttaa gtattctgaa catatgctta gaatatgtag atttaaggtg
     1261 tgtcatttct ctaagttcaa atttagtgaa gttactagtt aatgaattaa taatatgttc
     1321 taaatctgga ttaacaccaa tagccacata ctctttatct ttataaattt ctactttagt
     1381 aaaaagaata aattttctaa taacattctc agtttcttct ctatatgtta aagagaaaac
     1441 tttatcatag aatttttcta aatcttgaat aaaaagcttt ttatcatgac tatagtaatt
     1501 acttaattct tttaattctt caaatggcac attaacagtc aaattgcctt tatcttttaa
     1561 tttattacac aaagtaaaaa ataaattaac ttcagtatta ttaaattttc tcaatggaac
     1621 taaattcata tcatttttat atactactgt ttctccactc aactaactcc actccaatct
     1681 aaaagaaacc tttatagaat aaagattcct attaacttta attgaaaata cttataataa
     1741 ctattttata aacaataata acataaagaa acctaatata aaaaaagttt ctttagttta
     1801 atgattggtt gcctttagtt taatgattgg ttgcctttag tttaatgatt ggttgccttt
     1861 agtttaatga ttggttgcct ttaacatctc aaatccgttg tcactctgag gatcaaatcc
     1921 ctcctaaaaa ggttttaaaa agatttataa aaagatatta aaaagttcgg ggcaatttgc
     1981 ccctcacaaa ttcaaaagac aaaaaggaaa ccaaccacac ttgtggttgg tttttattat
     2041 ttaaaaaagc aataagtaat ataactttaa tgcattattc caattgcttt attgacattg
     2101 agcctctgaa cccttaataa acccaaaaac tgtcgactcg tcggtttcat agctcacgct
     2161 ataccgacat tcgtcgaaac agtttaatta agggttcttc tcaatgcaca ataaattttc
     2221 tcggcataat ggcgtgtgtt actttttatt tctcttttca tttctaagct attttaagct
     2281 tgatacagct tttttattac ctcaaatgtg gtttttattc aacatttata ttaaatgcgc
     2341 ttatacacgc ttaaaatggc atatataagt cctccaaata aagcataagg aacaagatag
     2401 aaaataagta tgaaataacg ccatgctgaa tgacctgttt tcaggacata gttcagccat
     2461 tcataaaaat gttgcacacc taaccagtcg cctaagggac ttaagactaa aaatgtgacc
     2521 atgactaaag caataataaa aaacatcgct gtaatgcctt tggttaaacg atgaaacgct
     2581 tgatcatatt gtttaacagc tgtttcgttg tgcttgatac gttcatccat ggttgcttta
     2641 taatcgttat tcgcttgttg cagttctgct tgattggttt gtaactgctc aatcgcctta
     2701 tgtgtgtctg tttttacatc tttcaattct tcttggatgg ctgtttgaaa atcttgtttt
     2761 aaattatcgt tattaatgcg ttcaatatag tgtttcgcta cactgataaa acgcttttgt
     2821 gtgatttcag tcgcttgatt aaagttcgag gccgttttct cgatcgttgt taataattcg
     2881 tcgtgacgct tgtttcgctc gttctctctc ttctcgatct cttcgagcct tttcatcagc
     2941 aagtcttgct gattctgcag cttgtctgct acgctcaagc tcatttgtgt cttgggcgaa
     3001 ttggtcccaa tctgattgag tgacgcctgt atctcgttca gttgattcat cacgacttga
     3061 agttggtcgt tttggctggc attcggtgtc tgagtctcgt tcttgttgtc tccgtcgttg
     3121 aatttgtctt tcaaagccat cttcgatacc tcctttattg taatcttcgc cgagtttacg
     3181 cccacgcacc tttttatctt cggctaagtg tcgataggta atgcttttgg gtgtgacacg
     3241 ttcaatttca ataccatgtt ggtttaaatg gtctttgaat tcattcatat tggtggcttg
     3301 tgattggtct atcgcctctc tgatttcatc tttccatgaa tattgggctg ttgatttcgt
     3361 attagggtca gcaatcgctt tttctgtctg cgtataacgt aagcgtgctg tgtccttttc
     3421 tggcacgctt aaaccatgtt ctatgcaaac ctcatcattg aaatttctga cattacgtaa
     3481 cgcttgttta ttgttattga atttcttacc tgtctcgagg tcaattgaat taatgacaat
     3541 atgattgtgt acatggtctg tatcggcatg cgtatagacc gccacttgat gattcggtgc
     3601 gagcttttca gcgagtgcta agcctaattg attacattgt tcgggcgtga cttcgccagg
     3661 tttaaacgat tggattatga catgtccttg attgccatcg gttttaccat ataattcacg
     3721 tgaagcttta aaactcgatt tcgcatagtc gacatcacaa tttaagccgc ttttttcaac
     3781 ggctcgtttc tcagcataat taatggcccg tgatgttgat ttggtggcac ttaatttagt
     3841 tgttgccatt ggtatcacgc tttttttgtt taggaataaa atgatcaatt aaaaggccac
     3901 cgaaagcaca aaataagaaa cagatggtga taaaccacca ttgttttagg aatgaaaaat
     3961 taaaaaataa agttaaaatc attaaagata taacagttaa aatccaaatg ctaaatagca
     4021 atcttcttaa tttagtttgt tccatacctc atctaacctt tcacgtaatt cgctgatatt
     4081 acgttctaat gctttatagt tcggtgcttc atgttggtgt tggttgcaat atttcgcaat
     4141 ctgattcgca tttgcgccca acatactcaa atctttcgct accgattgtc gcgttgcttg
     4201 atccaatttg ggtgcgacca atcgggcgcc ttgtgccttt ttcttaacga aagaaggcac
     4261 actcatattt aaagtttcag ctgattgttg taactttaaa tattcggatt cgctcacacg
     4321 aaaactgatt tgtttcggcg ccttacggtt gggtttatgg tttcgcccaa cagtttcgtc
     4381 gctagccaca aatgtgtttt gttcgctcat tattcccacc acgctttctt cgaaagataa
     4441 cgcctttctt acattttact ttataggata aagtaaaatt aatcaagtcg ttaagtggtt
     4501 tggcaagcac tggctttgct agccactcac gtgtcagacg cgccagcttg ttggggatac
     4561 tccccaagcc ccttaagatt ttttctgacg aaaaaaagct aacaaatgac atacgcctca
     4621 tcaaacaaat tcgtttggaa atctgtatgt caaatgttag ctgaatacac ctcgaaaaat
     4681 taatgtacat tagcattcat atgtatttta tattaatata aagaaaagca tacatatgtg
     4741 tgcttatcat atattaatca tgaggtggtc aaatgacaaa aaaacaaagt tcaaaagaaa
     4801 aaattttaac agttcgtgta agtcaagagc aatacaaaaa gatacaagat ttagcataca
     4861 tacgtagtat gaatacaaca gaatacatac gccaaaccgc tgtgggtaat cgtattaaac
     4921 ctacggttat tgagtatcct caagaacaaa caacgattga tgattataat tctaatggtt
     4981 ctgttgcaaa gtaaaaaaat atagctaacc actaatttat catgtcagtg ttcgcttaac
     5041 ttgctagcat gatgctaatt tcgtggcatg gcgaaaatcc gtagatctga agagacctgc
     5101 ggttcttttt atatagagcg taaatacatt caataccttt taaagtattc tttgctgtat
     5161 tgatactttg ataccttgtc tttcttactt taatatgacg gtgatcttgc tcaatgaggt
     5221 tattcagata tttcgatgta caatgacagt caggtttaag tttaaaacct ttaattactt
     5281 tagccattgc taccttcgtt gaaggtgcct gatctgtaat taccttttga ggtttaccaa
     5341 attgtttaat gagacgtttg ataaacgcat atgctgaatg attatctcgt tgcttacgca
     5401 accaaatatc taatgtatgt ccctctgcat caatggcacg atataaataa ctccattttc
     5461 cttttatttt gatgtacgtc tcatcaatac gccatttgta ataagctttt ttatgctttt
     5521 tcttccaaat ttgatataaa attggggcat attcttgaac ccaacggtag accgttgaat
     5581 gatgaacgtt tacaccacgt tcccttaata tttcagatat atcacgataa ctcaatgcat
     5641 atcttagata gtagccaacc gctacagtga taacatcctt gttaaattgt ttatatctga
     5701 aatagttcat acagaagact cctttttgtt aaaattatac tataaattca actttgcaac
     5761 agaaccttat aatccgtgac taatgttgaa agattcatta aaggcatcga taattgcatc
     5821 acataaaaaa tcatcgcaat taacgcacca gctgttatcg cacctgtagc tattctaata
     5881 gcaccaaaac ctaatataac cgcaatcatt aataacataa tcacacttga aatgggttga
     5941 atgacagctg taatttttgc ttgtcgcaga cctaaattat aaatttcacg taaattacta
     6001 tgagcgttgt ttaattcttg tctttctgta ttcgatactt ttactaatcg catctcagtt
     6061 aacacacgac ctagcaatcc actaaagtta gcgatttcag tttgcgtatt cattgatatt
     6121 ttttgcattt tgcgacctaa tggaatcatt attgccataa atatcggaat tgcaataaat
     6181 gttaataatg tcatttgcca gtccataatg aacaacataa ctatggatcc aatgagtgta
     6241 attactgcgg gaagtaattg tggcaatttc tgtgaaataa attcatttat aactttagta
     6301 tcgtctgtca aacgactcat taactgccca ctttcattct tatcgaaaaa aggcattttt
     6361 aatttaatga tatgttccca aagtattgat cgaatatcgt aaatcatttt ttcaccaatt
     6421 ttacttagta gataaatacc aataccactt agtagtgcat tagctataaa tattcctgca
     6481 aataaaagaa cgatattcca gttgatagta cttgttgaga atttatcaac taatcgtccg
     6541 gtaaatagcg gaattaacag accactaata ctccctagcg aagaaataat gattgccgca
     6601 ataatcagtc catatggcca cgacaatcgt ttaaataaga aaaataacgg attttgtcct
     6661 ttcataaatt agccctcttt ttatattcat tttaaaattt taattatctt attttccaag
     6721 tataaagctt taacaaataa attcatatta tcaaatttat atgataaaat attttagttg
     6781 aaattttgaa aaaggaaaga taagaatgaa acgaagctta ataattacct ttgctctact
     6841 tttaaccatt gttattatag cacctaactc ctacgcttcc gaatatgaac caacacctac
     6901 tgaaattgct agacaatatg gttacccgaa tgtaacagat gcctatcaac ctgaaggtag
     6961 cataaatgta agtcaaaccg ggcaaatttt gtacgactat caatctaacc aaaaatggta
     7021 ccccgcttca atgacgaagc taatgacaat gtatcttaca cttgaagccg tgaataaagg
     7081 tgagctttct ttaaatgaca aagtacatat tacggacaag cattatcgca tgtcaacttt
     7141 accagaatta agtaatacga aattatatgc tggtgaaaca tatacaatta gcgagctact
     7201 ccaaattaca gtttctaact caagtaacgc tgcagcttta attttagctg atgaggtttc
     7261 gggaaatgtg aatgatttta ctgatttaat gaataagaaa gctaaagcat taggcatgtc
     7321 aaacacccat ttcgtaaacc caacaggtgc tgaaaacaag caattaaaag aatttgcgcc
     7381 atcaaaatat aaaaaccaag acaatactac ttccactgcg aaagactttg caattctaga
     7441 tcaccatgtc atcaaagaga ctcctaagat attacatttc actaaacaac ttgctcctac
     7501 acaacacggt gtcacatatt atacattcaa tcattcatta gaaggagcca aaatgagttt
     7561 gctaggtaca gatggtttaa agactggctc aagtgatgtt gcagattata atcacacgat
     7621 tacaactaaa cgtaacaact tccgcattaa ccaagttatt atgggagcag gagattacgt
     7681 gaattttggt ggagaaaagc aacgtaatat gatgggaaat gccatgatga atcgttcttt
     7741 cgatcaatac agttataaaa aggtactttc aaaaggcaca cataaaatca atggcaaaaa
     7801 atactatgtc aaagataact tatatgatgt agtacctaaa ggtatgaata aaaaagatta
     7861 taaattcatc gttaaagatg gttcaataca tcttgactac aatcgccaat ttcttacaaa
     7921 agatgatgga cctccaaaag ttgaagtaac gaaacctcta ttgcacaaag caaataccat
     7981 tgctcaaacg acgtggaaag agcatccagt ggttaccttt gtcgcattag ccttattagc
     8041 aattgcattt atacttatgg tacgttcaat tattcattta ttatttaaac gtaaataaag
     8101 tgattgataa ctctttagct atatagtaac aatatctttt actttcagta ttaatataag
     8161 gaacctaagt gctatgtcac ttaggtaaga aattctaaaa tgtaaaacaa ttaagaattt
     8221 ctttaaagaa ctgctataga gacgtcacct caggaacgcg aaacaactta agttaaacaa
     8281 agaagccagc aaatgaattc ataaaattca tttgctggct cttttgatag gattaagacc
     8341 ttatattttt aattatttag cgtccttgcg gttctgttgc aaagtaaaaa aatatagcta
     8401 accactaatt tatcatgtca gtgttcgctt aacttgctag catgatgcta atttcgtggc
     8461 atggcgaaaa tccgtagatc tgaagagacc tgcggttctt tttatataga gcgtaaatac
     8521 attcaatacc ttttaaagta ttctttgctg tattgatact ttgatacctt gtctttctta
     8581 ctttaatatg acggtgatct tgctcaatga ggttattcag atatttcgat gtacaatgac
     8641 agtcaggttt aagtttaaaa cctttaatta ctttagccat tgctaccttc gttgaaggtg
     8701 cctgatctgt aattacctt
//
