LOCUS       CP053010                8470 bp    DNA     circular BCT 10-FEB-2021
DEFINITION  Staphylococcus epidermidis strain HD104-2 plasmid pHD104-2_3,
            complete sequence.
ACCESSION   CP053010
VERSION     CP053010.1
DBLINK      BioProject: PRJNA546129
            BioSample: SAMN14734458
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buttner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Distinct clonal lineages and within-host diversification shape
            invasive Staphylococcus epidermidis populations
  JOURNAL   PLoS Pathog 17 (2), e1009304 (2021)
   PUBMED   33544760
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buettner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2020 15:54:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,340
            CDSs (total)                      :: 2,269
            Genes (coding)                    :: 2,207
            CDSs (with protein)               :: 2,207
            Genes (RNA)                       :: 71
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 41 of 62
            Pseudo Genes (incomplete)         :: 23 of 62
            Pseudo Genes (internal stop)      :: 24 of 62
            Pseudo Genes (multiple problems)  :: 20 of 62
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="HD104-2"
                     /isolation_source="nose; prosthetic joint infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pHD104-2_3"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2018"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            234..653
                     /locus_tag="HKH64_11655"
     CDS             234..653
                     /locus_tag="HKH64_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002511307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRL18149.1"
                     /translation="MPKKQNSKEKILTIRVSQSQYEKICELADIREMNVTEYIRHTAIG
                     NRIKPTIIEQIKESSEVSELFKKDNEALKSNNQALQHHIHQLEDEIDQLRQENDVFHHL
                     LQHFDSTAFLNFNTYRDDRPLKNAIKRLKEQYKSK"
                     /name="hypothetical protein"
     gene            complement(688..2067)
                     /locus_tag="HKH64_11660"
     CDS             complement(688..2067)
                     /locus_tag="HKH64_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="QRL18150.1"
                     /translation="MNSSKYFDHLVVFTAGIGMFLSTLDSGIINVALPTLSKSFNVDAS
                     FISWSITLYTLLLTGTIIIFGRLSDKYNRLNIYSIGLTVFLISSILCGFSNNVVELITF
                     RGLQGIGAAMLQGTATAIITTTIPEHRQGPALGTLSILLGIGPVLGPSIGGLLISIGNW
                     RWIFWINIPFIFIGLIGCLLLKKYIKEPKSVSIHLDIRGNSLLFVSIFCLLVSLTSWSH
                     HSFFNISVYSNFLIFIITFCLFIVWELKTNHPIINLRLFKNISFSSSIFAIFVFGGTTS
                     LGFIIPPYILEKINHLSSWQIGLVNLTSPLGLVLISKITGKLISRVGNTILMTVGLVIM
                     IASYTSLGLLQYILNPITISLLLLIYGIGGGFFLPSNTSAIMGTVSKDMQGTVGATQRM
                     VQNIGIAVYTAITSLFISNQSQTNQLITGASHAWLFASATLFLSLLPFLLKLLHHKSEA
                     T"
                     /name="MFS transporter"
     gene            2179..2877
                     /locus_tag="HKH64_11665"
     CDS             2179..2877
                     /locus_tag="HKH64_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="QRL18151.1"
                     /translation="MYEKFADNILLIGHKTRLTMLIELSSGVSIPAGELAKLANVTPQT
                     ASEHLSKLVKANLISVNKKGRHCYYKIDNSEIVDAINALSIISPPMSSKSLKETTKKEK
                     LSYMRTCYGHIAGKIGVKFTEALLENDYLRESKDCYKLTQKGKFWLKKIGIATEKTMYS
                     KPIPKHIDWTERKNHIAGPIALQITEKLIELSWVKKSKINRCLNLTLKGKKAFKTYLNM
                     NITELENEIF"
                     /name="winged helix-turn-helix transcriptional regulator"
     gene            2996..3886
                     /locus_tag="HKH64_11670"
     CDS             2996..3886
                     /locus_tag="HKH64_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein MoeB"
                     /protein_id="QRL18152.1"
                     /translation="MNSKVIKYTADFNEKRYWERIKRNLGWLGDTDEEAKKRQKKISEV
                     VIGIAGCGGIGGAVAERLVRLGVRHIKVADPDHFELSNINRQFGASLDNLGRNKAEVVG
                     ESIFNISKDVNVDVYPEGINNSTADDFVEGCDYVLDKIELFELEARYALHKAFKKSERC
                     KFMLTVPVFGHRAFFFKWTKDSMSAEDYFGIKPGSELDEQNTRKIVYSLFPEYPQFPSK
                     ERLDDWLIYNKECPIFAGCPPLAQGLLVERLSFEIADLKDIPEYKELPSTPSYSMFDSA
                     SWETKIVEREENIID"
                     /name="molybdopterin biosynthesis protein MoeB"
     gene            3879..4802
                     /locus_tag="HKH64_11675"
     CDS             3879..4802
                     /locus_tag="HKH64_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020365845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="QRL18153.1"
                     /translation="MINNLLSMENLNLNDVNSLFEKADYYEKALKDKKVIKDLEGKILA
                     TLFYQPSTRTRMSFTSAMYRLGGQVIDMGGPKVARTDDYFDESMSDTIKYIEQYSDIMV
                     MRHFQNYTVEKASKITHIPMINAGDGTNEHPTQALLDLYTMTKELNGLEDKVIGIGGDI
                     NCRVIRSIVIGLEKFDIKKIIFLLPNGEELNSDILNLLKNTDYSIVHNVERVLEQADIL
                     DIIPFELPDFNSAYSEKVDEKPSLENNLIVSKEKFNDKNRIPILSPGPREAELSSDTDD
                     MDNVIFTKQAYNGLLIRMSLLYYFLH"
                     /name="aspartate carbamoyltransferase"
     gene            complement(5290..5433)
                     /locus_tag="HKH64_11680"
     CDS             complement(5290..5433)
                     /locus_tag="HKH64_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRL18154.1"
                     /translation="MSNKYNIHPALYIGGFAVFFSFIFGFPVGTLMGLAIGIGISKKNK
                     NL"
                     /name="hypothetical protein"
     gene            complement(5539..6405)
                     /locus_tag="HKH64_11685"
     CDS             complement(5539..6405)
                     /locus_tag="HKH64_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002452565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="QRL18155.1"
                     /translation="MSNETVIYHNEMNLVPLRKFTSVEIDLFFAMCNKLKEQDTNKLYL
                     SFEELKYLSNYNYETRNLNRFIGDLQNIYKKMLEITYRREDEDVIEFFVLFNHFKIHKT
                     EKYLEISTNPDLKFVLNDITRNFTKFELKELTNLKSSYSKTMFRLLKQYKHTGYLKLSM
                     EDFRTRLDVSKSYQMCDITKRVLKPISKELTPIFNNLNINKVKAKKGRKIEYLEFIFDA
                     EKRIHNKRQPQITNIGKSRQYTNREKTPKWLDDKIYKQSQESHGEDAKLKQDREAFQRQ
                     LEEKWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(7041..8249)
                     /locus_tag="HKH64_11690"
     CDS             complement(7041..8249)
                     /locus_tag="HKH64_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="QRL18156.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLKRTNLNFDLV
                     EDERHLYHRVKDRIEYYKKQGSRVQKNSVVMYSNIITLSKEEADRMGETRTKHYFKTCK
                     DYFSERFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     HLTRDIQNLKRKNHFLQEDSQQAQEDIDLYKQLRTISRDDFKKEEEKYQKEIKAYEASL
                     SGLRMNYRDLDKRNQHLTTQNNQLEHHLDVLNARHQAMNDMVRNDTRILFAGLRCALKD
                     MNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGGVSGIDHHIRKASVPIKKEAENDL
                     ELE"
                     /name="recombinase"
     ARG             complement(689..2067)
                     /name="mdeA"
                     /gene="mdeA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; aminoglycoside
                     antibiotic; penam; tetracycline antibiotic; disinfecting
                     agents and antiseptics"
                     /identity="40.29"
                     /coverage="101.32"
     Typing          complement(7045..8321)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.948"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgctctgg cgaggccatt acggcattca aaaatatatt tttgaatgcc gttctatagc
       61 taacaaatga catacacctt atcaaacaac tatgtttgaa aatatgtatg ccaaatgtta
      121 gctcatacca cctcgaatca tacatattta ttagcataca tatgtatgtt atattaatat
      181 aaaaataagc acacatatgt atgcttatca tatattaatc atgaggtggt tcaatgccaa
      241 aaaaacaaaa ttcaaaagaa aaaattttaa caattcgtgt aagtcaatcg caatacgaaa
      301 agatttgtga acttgcggat attcgagaga tgaatgtgac cgaatacata cgtcatacag
      361 ccattggtaa tcgtattaaa ccgacaatca tcgaacaaat taaagaatcc agtgaagtca
      421 gtgaattgtt taaaaaagac aacgaggcac tcaaatcgaa caatcaggca ctccagcatc
      481 atattcatca gttggaagat gaaatcgacc aactgagaca agaaaatgac gtctttcatc
      541 acttattaca acattttgat agtacagcat ttttaaactt caatacatat cgtgatgatc
      601 gtccactaaa aaatgcgatt aaacgattaa aagaacagta taaaagcaag tgatttataa
      661 ttttataaat cacttgcttt tattatatta agtagcttct gacttatggt gtaataattt
      721 taataaaaaa ggtaatagtg ataagaaaag tgttgctgat gcgaataacc atgcgtggct
      781 ggcaccagtt atcagttgat ttgtctgtga ttgattactt ataaatagtg aagtaattgc
      841 tgtataaact gcaataccta tattttgaac catccgttga gttgctccaa cagtaccttg
      901 catatcttta gatacagttc ccatgattgc tgaagtatta gatggtaaga aaaatcctcc
      961 acctatacca tatatcaata acaatagact tattgttatt ggatttaata tgtactgtaa
     1021 taaccctaag ctagtatatg aagcaatcat aataactagt ccaacagtca ttaaaatagt
     1081 gttaccaaca cgagatatta atttccctgt gatttttgat ataagtacta atcctaaagg
     1141 agatgttaaa ttgactagtc ctatttgcca agatgaaaga tgattgattt tttctaaaat
     1201 atatggagga attataaagc ctaaagacgt tgtacctcca aaaacaaata ttgcaaatat
     1261 agaagatgaa aatgatatgt ttttgaataa acgtaaattg atgataggat gattagtttt
     1321 tagttcccac acaataaata agcaaaaagt tataataaat attaagaaat tgctatatac
     1381 tgatatatta aaaaatgaat ggtgagacca agatgttaaa ctcactaata agcaaaatat
     1441 agatacaaat agtagcgaat ttccacgtat atctaaatga atagatacac tttttggctc
     1501 tttaatatat ttttttaaaa gtaaacagcc tattaagcct ataaatatga atggaatatt
     1561 aatccaaaat atccatctcc aatttcctat agatattaaa aggcctccaa tactaggacc
     1621 taacacggga cctattccta ataaaatgct caaagttcct aatgctggtc cctgtctatg
     1681 ctcaggaatg gttgtagtaa taatagctgt agcagtgcct tgtaacatag ctgcacctat
     1741 gccttgtaaa ccacgaaaag taattaattc taccacgtta tttgaaaatc cacataaaat
     1801 agaagatatc aaaaacactg ttagtccaat actatagata tttagacgat tgtacttatc
     1861 actcagtctt ccaaatataa taatggtccc tgtaagtaac aaagtatata acgtaatact
     1921 ccaagaaata aaagaagcat caacattaaa agatttagat aaagtgggta acgccacatt
     1981 gataatacca ctatctagag ttgataagaa catacctatt cctgctgtaa aaacaactaa
     2041 atgatcgaaa tacttagacg aattcatcta tcatacctcc tcattcaact aatttaatca
     2101 tacccatata atatttcgtt taacaccgaa atattatatg ggtatgatta aattgtacta
     2161 atagaaagga ggcattatat gtatgaaaaa tttgctgata atattttact tattggacat
     2221 aagacacgtt taactatgtt aatcgaatta tcaagtgggg tatctatccc agctggagaa
     2281 ttagctaaat tagctaatgt aactcctcaa acagctagtg aacatctttc aaaattagta
     2341 aaggccaatt taatttcagt aaataagaag ggacgacatt gctactataa aattgataat
     2401 agtgaaattg ttgatgcaat taatgcttta tctattatat ctccacctat gagtagcaaa
     2461 tcgttaaaag aaacaacgaa aaaagaaaaa ttatcataca tgcgtacttg ttatggtcat
     2521 atcgctggga aaataggtgt taaattcact gaagctttgc tagagaatga ttatcttaga
     2581 gaaagtaaag attgttataa gttgactcaa aaaggaaagt tttggttaaa aaaaataggt
     2641 attgcaacag aaaaaactat gtattcaaaa cctattccca aacatataga ttggacagaa
     2701 aggaaaaatc atatagcagg tcctattgca ttgcaaataa ctgaaaagtt gattgaatta
     2761 tcttgggtta aaaaaagtaa aatcaataga tgtcttaatt tgactttaaa aggaaaaaaa
     2821 gcttttaaga catacttaaa tatgaacatt acagaattag aaaacgaaat attttgacaa
     2881 ttaaaagagt tttatgatta tgtagttatt gtaagtgtat taaaccactt atattttaat
     2941 ttttctgtat cgagttatgt atttattata aaacactatt ttttggaggt ttataatgaa
     3001 ttctaaagtt attaaatata ctgctgattt taacgaaaag cgatattggg aaagaattaa
     3061 acgaaattta ggttggttag gagacactga tgaagaagcg aaaaagcgtc aaaagaaaat
     3121 tagtgaagtt gtaataggta ttgccggatg tggaggtata gggggtgctg tagctgaaag
     3181 actagtacga ttgggtgtcc gtcatataaa agtagcagat ccagatcatt ttgaactctc
     3241 taatattaat agacagtttg gcgcctcttt agataatcta ggaagaaata aagcggaagt
     3301 agttggagaa tctatattta atatttcaaa agatgtaaat gttgatgttt atcctgaagg
     3361 aattaacaat agtactgcgg atgattttgt agagggttgt gattatgtat tagataaaat
     3421 tgaattgttt gaattagaag ctcgttatgc actacataaa gcttttaaaa agagtgaaag
     3481 atgtaaattt atgttaactg taccagtatt tggtcataga gctttctttt ttaaatggac
     3541 taaagactca atgtcagccg aagactattt tggtataaag ccaggttcag aactggacga
     3601 acagaataca agaaaaatag tttactctct tttcccagaa taccctcagt ttccatccaa
     3661 agaaagatta gatgattggt taatttataa taaagaatgt ccaatttttg ctggatgtcc
     3721 ccctttagct caaggtcttt tagtagaaag attaagcttt gaaattgctg atttgaaaga
     3781 tattccagaa tataaagaat taccttcgac tccaagttat tctatgtttg attcagcctc
     3841 ttgggaaaca aaaattgtag aaagagagga aaatataatt gattaataat ttattgtcga
     3901 tggaaaattt gaatctcaat gatgttaata gcctatttga aaaagcagat tattatgaaa
     3961 aagctttaaa agataaaaaa gtaattaagg atttagaagg taaaattctt gctacgcttt
     4021 tttatcaacc aagtaccaga actagaatga gttttacaag tgccatgtat cgattaggtg
     4081 gacaagtcat agatatgggt ggacctaaag tagcacgcac tgatgattat tttgacgaga
     4141 gtatgtcaga tactattaaa tatattgaac agtattcaga catcatggtt atgcgtcatt
     4201 ttcaaaatta tactgttgaa aaagcatcga agatcaccca tattcctatg attaatgccg
     4261 gagatggaac aaatgagcac cctacgcaag ctttattaga tttatatacg atgacaaaag
     4321 aattaaatgg tttagaagat aaagttatag gaataggtgg agatattaat tgtagagtga
     4381 ttcgttctat tgttataggt ctagaaaaat ttgatattaa gaaaattata tttctattgc
     4441 caaatggaga agaattaaat agcgatattt taaatttatt aaaaaacact gattattcta
     4501 tagtccataa tgtagaaaga gtattagagc aagcagatat attagatatc ataccttttg
     4561 aacttccaga ttttaattct gcttattcag aaaaagttga tgaaaaacca tcattagaaa
     4621 ataatttgat agtatctaaa gaaaagttta atgataagaa taggatccct attctaagtc
     4681 ctggaccaag agaagctgaa ctttcttcag atacagacga catggataat gtgatattta
     4741 ctaaacaagc atataatggt ttattaataa gaatgtctct tttatattat tttttacact
     4801 gattaatagc tatttataaa atgaatacgc tatacaaaag cactacccat aatcgttaat
     4861 tatgtcaact aacaataaag gcttaaacgt tgatttaaca gcgtttaagc ctttgtcttg
     4921 tgattatcgt ttattcatct tttatacatc gttgttctca cgtgttttca tactttttca
     4981 tttatagata ttttgtatct ttattcacaa tttcacgtga taacacaccg ttttaaattg
     5041 tagttttttg ttatactgga aaagcacttc cgtgcacaaa ggaggtgtca catacaatga
     5101 taatcttgct atttgtatta gataagctca tttttccaat tattgttggt tatgttcttt
     5161 atctattaca acaagaacat aagtaaaaag aaacccccag ccggcacgct gggggttttc
     5221 acatacaatg atatatctgc tatttgttaa ttacagtata tcgtgtaact tcttcatctg
     5281 taaactcttt tacaaatttt tatttttttt actaattcct attcctatag ctagtcccat
     5341 taatgttcct acaggaaatc caaatataaa actaaaaaat accgcaaatc cgcctatata
     5401 tagtgctgga tgtatattat atttattact cataccttct tatatctcct tagtcatgtt
     5461 ttttcttttt tattgatacg ataaatccca ttattaatgg aaataagatt aaaatataca
     5521 tgtagctcat ttaaattatt attcctccca tttttcttct aattgacgtt gaaatgcctc
     5581 tcgatcttgt tttaattttg catcttcgcc atgtgactct tgagattgtt tatatatctt
     5641 gtcgtctaac catttaggtg tcttttcacg attagtatat tggcgcgact tacctatatt
     5701 agttatttgt ggctgtcgtt tgttgtgaat acgtttctca gcgtcaaaga taaattctaa
     5761 gtattcaatt tttcgacctt ttttagcttt aactttatta atattaagat tattaaaaat
     5821 aggtgtaagc tctttagata ttggctttaa tacacgttta gtaatgtcac acatttgata
     5881 agactttgat acgtctaatc gagttctaaa atcttccatt gataatttta aatatccagt
     5941 atgtttatat tgtttaagta agcgaaacat ggttttagag tacgaagatt ttaaattagt
     6001 cagttctttt aattcgaatt ttgtaaaatt acgagtaata tcatttaaaa caaactttaa
     6061 gtcaggatta gtggaaattt caagatactt ctccgtttta tgaattttaa agtgattgaa
     6121 aagtacaaaa aactctatta catcttcatc ttctcttcga taagttatct cgagcatctt
     6181 tttataaata ttttgtaaat ctccaataaa acgattaaga tttctagttt catagttata
     6241 attacttaaa tatttaagtt cttcaaaact taaatataat ttattagtat cttgttcttt
     6301 tagcttatta cacatagcaa aaaataagtc tatttcaaca gaagtaaatt ttctcaaagg
     6361 aaccaaattc atttcattat gataaataac agtttcgttc gacatatata tcactccaaa
     6421 aaaggtcgtt atttttataa gtccttgtat atcatataca tattatcact ataatagttt
     6481 tattaaaagt atttgccaca taaaaaaggt cattatttta atttaacctt ttttatgtgg
     6541 caaaatgacc ttttttatgt ggcaaaatga ccttttttat gtggcaaaat gacctttttt
     6601 atgtggcaaa atgacctttt taagttgtgg aatctactgt cgctctaagg attaaagcac
     6661 acctaaaaag gtttataaaa agatttataa aaatatatta aaaaggtcgg agtgaaatac
     6721 tccaaattca ttcaaatata cttctaaaga aacgaacaca aaaaaaataa actttaaaaa
     6781 atatcttcat caattaatct agaaatcata ctcacatcta tcatttccaa tacaatatct
     6841 ttgctacctt agaattctta taataatata gaaatgcaaa ggtattgttc ctccttacag
     6901 aactatttaa taagcgctgt aagtaaggta atgattgttc ttaatcggtt aatcatactt
     6961 attaaatttt tatctcctct tttcatgaga taagcactca ggcgagtatc atacttgttt
     7021 gagtgcttat ttttattgat ttattctaat tctaagtcat tctctgcctc ttttttaata
     7081 ggcactgatg cctttcttat atgatggtct atccctgaca caccaccttg atatgcatgt
     7141 gttaaagccc cctgatccac cgtatcggga tcaataccga cttctggcat acgttgatat
     7201 ttaatttctg cgcttaattc aatgttcata tctttcaatg cacatctaag tcccgcaaac
     7261 aaaatacgtg tatcatttct taccatgtcg ttcatcgctt gatgacgtgc atttaacacg
     7321 tctaaatggt gttcgagttg attattttgt gttgttaaat gttgattgcg cttgtccaaa
     7381 tctcggtaat tcattctaag gccactcaga gacgcctcat acgctttgat ttctttttgg
     7441 tacttttcct cctctttttt aaaatcgtcc ctagaaatcg ttctcagttg cttgtatagg
     7501 tcgatatctt cttgagcctg ttgagaatcc tcttgtaaaa aatgattttt ccgttttaag
     7561 ttttgaatat cccgagttaa atgttctttt tcatcgtgta aatcgttgat atccctcaac
     7621 aattgttttt tcgtttcaat ttgttggtct aattcactta aaactttcgc tttcttttta
     7681 tattcatgaa tatcttgaat atacgtttta ttatcatcag tactcccacg ttcaacatca
     7741 aaaccttgct ggcacagatg tgttgttaat tcatcatgga tgtgattaat ggcttgtcga
     7801 ttcatagctg tacgtgctga taaccgccct tgatgattaa caggtataaa gtgaaggtgc
     7861 atatgaggcg ctgattcatc catatgcact ttcgctgaaa cgacattggc ttcaccaaaa
     7921 cgttcactga agtaatcttt acatgtctta aagtagtgtt ttgtacgcgt ctctcccata
     7981 cggtcggcct cttctttcga taacgtaatg atattggaat acatcaccac actatttttc
     8041 tgtacacgtg agccttgttt tttatagtac tcaattctat ctttcacacg atggtacaaa
     8101 tgacgttcat cttcaactaa atcaaagttt aagtttgttc gtttcaaatc aatctcatcg
     8161 tttttgattc tccctttacg ttcttgttcc atctctttgc ctttaacatc gctcatttta
     8221 tttttttgcg tattccaagc taatttcaaa ttgagcgccc tccccgaagc tttttgattt
     8281 gtctgtggtt ctctactttc gagaaccttc gcaaatatca ttttacaagc gctgtgattg
     8341 tctaccttcg acaatgcttc gcttatgtaa aatgatggat actctcaccg ctacgcctca
     8401 aacatgtgag gctgattccg caatgagagt atagctcgat atactcttac attcaaatgt
     8461 aagagttatc
//
