LOCUS       CP053009                8812 bp    DNA     circular BCT 10-FEB-2021
DEFINITION  Staphylococcus epidermidis strain HD104-2 plasmid pHD104-2_2,
            complete sequence.
ACCESSION   CP053009
VERSION     CP053009.1
DBLINK      BioProject: PRJNA546129
            BioSample: SAMN14734458
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buttner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Distinct clonal lineages and within-host diversification shape
            invasive Staphylococcus epidermidis populations
  JOURNAL   PLoS Pathog 17 (2), e1009304 (2021)
   PUBMED   33544760
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buettner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2020 15:54:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,340
            CDSs (total)                      :: 2,269
            Genes (coding)                    :: 2,207
            CDSs (with protein)               :: 2,207
            Genes (RNA)                       :: 71
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 41 of 62
            Pseudo Genes (incomplete)         :: 23 of 62
            Pseudo Genes (internal stop)      :: 24 of 62
            Pseudo Genes (multiple problems)  :: 20 of 62
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="HD104-2"
                     /isolation_source="nose; prosthetic joint infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pHD104-2_2"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2018"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            join(8056..8812,1..2510)
                     /locus_tag="HKH64_11610"
     CDS             join(8056..8812,1..2510)
                     /locus_tag="HKH64_11610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="QRL18147.1"
                     /translation="MITEENYRSNGMNIFIRRTNLKLTYKGRQVSFKINKKGIVTGVSH
                     GGHYQTHGGLPGMTPFLTPPNYFLNPALFFRVSGLIDYYVKNDITPLWVGENGSCSKVV
                     EGTWKYFIKKYKESKKVIGNNGLMNRVPSGLVDVRDFVLRYCNKVYLDFGFDTEWYHDD
                     RYILCYQFSTTLDDIQYDFVFHVVDKCRLSLGEMIYHIVSNTFAGRGFVILNEKLLHHK
                     TPFYESCNIRLIAHFAGVDFSMCNDYKEILKYLKHSRSQEQQLASIKLQLKDGKDKKGR
                     SLTDDDKDKLKKRYNKINENPVHATNMISSDKHCLYTVNPRVYNFWVIPRRQRCFFKLD
                     MRDTMKLAPPGSPLASLGESLGIEKMDTNVLDKQDNKPLNFYKENMKELRKNHFDFFVD
                     YAIRDARISYEWYQNVKNIIGDGVTVSAIAAKHLTDNVKKEMLKYYNEYSWDKHARGFK
                     LEDGKPNYNLFYKSRGMFGDLPDYAYIGGRNESFSHGLITGRTYDYDLRSAYPAQMQVM
                     RMIDFDKDPIVFNEGHVLTHDDWLHIGQAGFGYVHFEFPKSVKYPTIPLKSDKPNLKGS
                     PVFLRKGYGLVSAPDVYAAIQVGATVTVGKQGFMFFDLKRASEVDALEDFNELDVDNDF
                     IMHPVGFGVRSMIQIRADFAKKYGKKSVQAQLMKILVNSTYGKTGQGIHGNTARNILTD
                     VSEVIPYSKITDGVIASTTTSLVRCVLGMIMNYIADHGYTVHSVTTDGFISDFPWEKML
                     EIDDYLASISTVYQKVIKACWGEDLEYKTGKGSILEVKHIQDDYFFNIKTRGNISLDKD
                     KSYINPFTNETVTFDGVFAKAGYKGDKSFKYLSDFEKRKFLLNEVLNRDGKLIDMQSDM
                     LSFRDVKNNSLKSITKHYDHIDDIKKGLSMDYDKKRMLQEREYSSIKTKEFKSDYGFFP
                     TRPFEDMAEYEYNADVHSAFIKMGHNIKSDIGFLGYKKALEHGFPKFKKVDKLHHLTSN
                     ELKDYKDNFKKYEVRQFLIYMLKHGGLQRKSLFSLDELKRKDYLKLIDEVYGINALDMW
                     KNIAKRDKHNFDYLTAIDVFNRLHKHYQFVYVEELEFEEP"
                     /name="DNA-directed DNA polymerase"
     gene            complement(2606..2848)
                     /locus_tag="HKH64_11615"
     CDS             complement(2606..2848)
                     /locus_tag="HKH64_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRL18140.1"
                     /translation="MLPSTLLWIITIATLIYIIYNIVFSKPFINVFVAIIIQSVLLFAI
                     RYFWQDKTFGDAFIHSFDIVTIVIVIIFGIFKLSK"
                     /name="hypothetical protein"
     gene            complement(2867..3385)
                     /locus_tag="HKH64_11620"
     CDS             complement(2867..3385)
                     /locus_tag="HKH64_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRL18141.1"
                     /translation="MKKIITASLVGTLLVTGTSTVVNAQTQTQSQQGNVQKQGTITNDV
                     KNDKRYQALSPQAKDSFKTLVEANNYNNTEQMELLNNTAQTKGMDKNGNTERGKIGITK
                     KVVKQAWNSLPTKVKKKIGTQSKFYAALKAVDHFTGDVEDEIYKQMKGLGLSDNYAWIA
                     TKAITLIIL"
                     /name="hypothetical protein"
     gene            3620..4237
                     /locus_tag="HKH64_11625"
     CDS             3620..4237
                     /locus_tag="HKH64_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QRL18142.1"
                     /translation="MSKIGYARVSTKEQNLSSQIEYLKQQGVSESLIFIDKGVSGTIRP
                     NERKGFKKLLQKSRGGDVLVVYKLDRISRKYDDVQTVIRQLKDDGLSFEIGGLPNINTG
                     NELMDKAMTDMLLSLLGYVAENERAYIRERQSVGIEIAKKEGRFKGKPKKYSADSKDRH
                     GRQVYYMIVNDLKEGHSMNSIRLKYNVGYGTIQRIAKELEDN"
                     /name="recombinase family protein"
     gene            4255..4668
                     /locus_tag="HKH64_11630"
     CDS             4255..4668
                     /locus_tag="HKH64_11630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRL18143.1"
                     /translation="MNQKVIELKQQYVNDTERIRHMISNKKKHIEEEKRKLVKHQNEFD
                     KAQLDYSKNASLKLLDMVAEGSIDASELNAIKEYLFGDSNDLDVTAEVTSNYVNTSKEV
                     SHTSSNHQAKTHHDNVTVDDEDDDDFPNPLTDY"
                     /name="hypothetical protein"
     gene            5041..5589
                     /locus_tag="HKH64_11635"
     CDS             5041..5589
                     /locus_tag="HKH64_11635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRL18144.1"
                     /translation="MTNINNLTDDELMRLYDEENNKVKALREAMKQRKKIQHKIDESER
                     KVKSINFRVTQNAYDELSQMCEVLPSFKSPNEVARSVLMEWLDKDKTFLPPRNTFDPET
                     FELLQNLYRVASELRNDMRGMANNYNQHVKHLNTLVNKRELTRSDIEEIKEFNTFRKTL
                     TKQVNNFNKEVGKLCLLYK"
                     /name="hypothetical protein"
     gene            5742..6833
                     /locus_tag="HKH64_11640"
     CDS             5742..6833
                     /locus_tag="HKH64_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QRL18148.1"
                     /translation="MREVQKEYGKDKGYVQGYSIIQGFGADEIDPKGGVNSRNKAHDLG
                     LQFAQQVYGENNQAIVVTQCDGKSGLWHNHIISNAVGLDGKSQRGNMTKWTQLAKINDE
                     IALENGLTPLDKDNWQKNKDRKRDAKAKGQYQTPIEKRMRKNGVVLAKDEVIEQIRIAR
                     DEPSVQNIDDFKQLLHEEYNIDVLEARAKDGENLRYSYEYTNKKGLTNTKVSRASTLAG
                     EEFQLTPLTKEIEKKVALQQAEKEKDPGLVDYGLDIEEELNTPQAQMMIQNLLQKDEEE
                     EEEELELEFSEEDLQVLNDIESEVKDYAREKERDTTVVRNNNRNQKQHSDYQHNKSRHE
                     TNTTRTKQTRRATKSKDDGFTLE"
                     /name="mobilization protein"
     gene            6826..7347
                     /locus_tag="HKH64_11645"
     CDS             6826..7347
                     /locus_tag="HKH64_11645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRL18145.1"
                     /translation="MSKETQQNIDILKKKLENLANETHEVKVNLERNDIYEIADAVSKS
                     LEIHLKTQLEDKIKEDENTLKDATRYLKRQADDIKKETEDLTKYKKWFITAMIGFVAFA
                     VVLLIFTTLVDMPFLEHAYQFIATKVEHSHKWYTTALWYIAYLITPLFWIGVVTGVAYL
                     LHKWYTSKWR"
                     /name="hypothetical protein"
     gene            7352..7807
                     /locus_tag="HKH64_11650"
     CDS             7352..7807
                     /locus_tag="HKH64_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRL18146.1"
                     /translation="MLTIKQLADKLGVSKPTITRNKPDDLEFEVIDGVNYINENMERQI
                     TEKVLQNKERYSGDTRNETYDTPLIQQLMQENAFLRAQIEEKDALIKEQTKLLDQQQQL
                     NLITGKRLNELETKEQTETEEQHSNETVTKHTVSEGKKGFFSRIFGN"
                     /name="hypothetical protein"
ORIGIN
        1 agtatcttaa gcatagtcgt agtcaagaac aacaattagc ttctataaaa ctacagttaa
       61 aagatggtaa agataaaaaa ggtcgttctt taactgatga tgataaagat aagttaaaga
      121 aacgttataa taaaataaat gaaaatcctg ttcatgctac aaatatgata tctagtgaca
      181 aacattgtct ttatactgtt aatcctcgtg tttataattt ttgggttatt cctagacgtc
      241 aaagatgttt ttttaagtta gatatgcgtg acactatgaa attagcgcct cctggttctc
      301 ctttagctag tttgggtgaa tctttaggca tagagaaaat ggatactaat gttttagata
      361 aacaagataa taaaccactt aatttctata aagagaatat gaaagaatta agaaaaaatc
      421 attttgattt ctttgttgat tatgctatcc gtgatgctcg tatcagctat gagtggtatc
      481 aaaatgttaa aaatattatt ggtgatggtg taactgttag tgctattgct gcaaagcatt
      541 taactgataa tgttaaaaag gaaatgctta aatattataa tgaatattcg tgggataaac
      601 atgctcgtgg ttttaaactt gaagatggta aacctaatta taatttgttt tataaatctc
      661 gtggtatgtt tggtgatttg cctgattacg cttatattgg tggtcgtaat gaaagttttt
      721 cacatggtct tatcactggt cgtacttatg actatgactt gcgttctgca tatcctgcgc
      781 aaatgcaagt gatgagaatg attgattttg ataaagatcc tattgttttt aatgaggggc
      841 atgttttaac acatgacgat tggttacata taggtcaagc tggttttgga tatgtacatt
      901 ttgaatttcc taaatctgtt aaatatccca caatcccttt aaaatctgac aaaccaaatt
      961 taaagggttc tcctgtattt ttacgtaaag gttatggact tgtatctgcg cccgatgttt
     1021 atgctgctat tcaagtaggt gctacagtta ccgtaggaaa acagggcttt atgttttttg
     1081 atttaaaacg tgcctctgaa gtagatgcat tggaagattt taacgagtta gatgttgata
     1141 atgatttcat tatgcaccct gtcggttttg gtgttcgtag tatgattcaa attcgtgctg
     1201 attttgctaa aaaatatggt aaaaaatctg tacaagctca attaatgaaa atattagtta
     1261 actcaaccta tggtaaaaca gggcaaggca tacatggtaa tacagcaaga aatattctta
     1321 cagatgtgtc agaggtgatt ccttatagta aaattactga tggtgtgatt gcctctacaa
     1381 caacatctct tgttagatgt gttttaggta tgataatgaa ctatatagca gatcacggtt
     1441 acactgttca cagtgtgact actgacggat ttatttccga ttttccttgg gaaaaaatgc
     1501 ttgaaattga tgattattta gcaagtattt caactgtgta tcaaaaagtc attaaagctt
     1561 gttggggtga agatttagaa tataaaacgg gtaaaggttc tattttagag gttaaacata
     1621 ttcaagatga ctatttcttt aatataaaaa cacgtggtaa tatctcactt gataaagaca
     1681 aatcttatat taatcctttt actaatgaaa ctgtgacttt tgatggtgta tttgctaaag
     1741 ctggatataa aggcgataag tcttttaaat atcttagtga ttttgagaaa cgtaagtttt
     1801 tacttaacga agttttaaat cgtgacggta aacttattga tatgcaatct gatatgcttt
     1861 catttcgtga tgttaaaaat aattctctca agtctattac aaagcattat gatcatattg
     1921 atgatatcaa aaaaggttta tctatggatt atgataaaaa acgtatgtta caggaacgtg
     1981 agtattctag tattaaaact aaagaattta aatctgacta tggtttcttt ccaactagac
     2041 cttttgaaga tatggcagaa tatgaatata atgctgatgt tcacagtgcc tttattaaaa
     2101 tgggtcataa tattaaatct gatattggtt tcttaggtta taaaaaagcg cttgaacacg
     2161 gattccctaa atttaagaaa gttgataaat tacatcattt aacctctaat gagttgaaag
     2221 actataaaga taattttaaa aaatatgaag ttagacaatt cctaatttac atgttgaaac
     2281 atggtggttt acaacgcaaa agtttatttt ctttagatga acttaaacgt aaagattatt
     2341 taaaattaat tgatgaagtt tacggtatta atgctttgga tatgtggaaa aacattgcta
     2401 aacgtgataa acataatttt gactatttaa ctgctataga tgtgttcaat agacttcata
     2461 aacattatca atttgtatat gtagaagaat tggaatttga agaaccttaa aaacctaaaa
     2521 atcagtctag gcgttagtcc tagacttacg tttttaggtt aaaagaaagg agctaaccct
     2581 aaaggttagc tcctttctgt gttctttatt ttgataattt aaaaatacca aatatgatga
     2641 caataactat agtgacgata tcgaatgaat gtataaacgc atcgccaaat gttttatctt
     2701 gccaaaagta acgaatagca aataacaata cagattgtat tattattgca acgaaaacat
     2761 taataaacgg tttagaaaac acaatgttat atattatata tataagtgtt gctatcgtta
     2821 taatccatag taacgtgctt ggaagcaaaa tatcatctcc agctgtctat aaaataatta
     2881 atgtaattgc ttttgtagca atccatgcgt agttatcgct taaacctaag cctttcattt
     2941 gtttataaat ttcatcttca acatcacctg tgaagtgatc gactgcttta agagctgcat
     3001 agaatttact ttgtgtaccg attttctttt taacttttgt tggtaaagaa ttccacgctt
     3061 gtttaacaac ctttttagta atacctattt taccacgttc agtattacca tttttatcca
     3121 taccttttgt ttgcgctgta ttattcagta gttccatttg ttctgtatta ttataattat
     3181 tcgcttcaac taatgtttta aaactgtctt ttgcttgtgg tgatagtgct tgatatcttt
     3241 tgtcattttt aacgtcattt gtgatagttc cttgtttttg aacattacct tgttgtgatt
     3301 gtgtttgtgt ttgtgcatta actactgtag aagttcctgt tactaataaa gtaccaacta
     3361 agcttgctgt aataattttc ttcatttttt acaagtcccc tttgttaata attttaaaaa
     3421 atacactctc aatatttatt atagtaatac tataatatga ttttgagact tttccctaag
     3481 tgtttatttt tatttcataa gtgtaaattg ttgtaattaa ttcacaaata aagtatactt
     3541 aaactgtgaa ttaaacatcg ttgttatgac agcatttaga ttcacatttt gagttcacag
     3601 ataagaaagg gttaaaacaa tgtctaaaat tggatatgca cgtgtctcaa ctaaagagca
     3661 aaacttatca tcacaaattg aatatttgaa acaacaaggc gtatcagaaa gcctcatatt
     3721 catcgacaaa ggtgtttctg gtacaatcag acctaatgaa cgaaaaggct ttaaaaagct
     3781 actgcaaaaa tcccgtggcg gtgatgtact cgttgtttat aaattagatc gtatttcacg
     3841 taaatatgat gatgttcaaa cagtgatacg acaattaaaa gatgatggct tatcttttga
     3901 aatcggagga ctacctaata tcaacacagg taatgaactt atggataaag cgatgactga
     3961 tatgttgtta agtttgcttg gttatgttgc agaaaatgag cgtgcataca tcagagaacg
     4021 tcaaagcgtc ggtatagaaa ttgcaaagaa agagggtcgt ttcaaaggta aaccgaagaa
     4081 gtacagtgct gactcaaaag acagacacgg aagacaagtg tattacatga tcgttaatga
     4141 tttaaaagag ggtcatagca tgaattctat acgtcttaaa tacaatgttg gttacggaac
     4201 gatacagaga attgcaaaag agttagaaga taattaagaa tcggagtgat ttaaatgaat
     4261 caaaaagtca ttgaattaaa acaacaatat gtgaatgata ctgaacgtat tcgtcatatg
     4321 attagtaata aaaagaaaca catcgaggaa gaaaaacgca aacttgttaa acatcaaaat
     4381 gaatttgata aagcgcaact tgattactca aaaaatgcat cattaaaatt acttgatatg
     4441 gttgcagaag gttctattga tgcaagtgaa cttaatgcta tcaaagaata cctatttggt
     4501 gatagcaacg acttagatgt gaccgctgaa gttacatcta attatgtgaa tacttcaaaa
     4561 gaagtttcac atacatcatc aaatcatcaa gctaaaacac atcatgataa tgtgactgta
     4621 gatgatgaag atgatgacga ttttcctaac ccattgactg attattaatt cagtcgtcaa
     4681 tggttaggtt gtatcacacc ttttgtgtga tacgtggtat aatgtaacta tgagttacta
     4741 ttttaccacc agggttttac caaggggatt aaaggggacg ctccgctccc ctttacaagc
     4801 acccaatgtt tacattggga ctgcttgcct atgattcaac aaggggtgct aaggtgctaa
     4861 gctttgccta tgattcaaca aggggtgcta agctttgctt atgtgccctg cagttgaatg
     4921 catttaacat aacacaattg tcgcaccttt tttgcgacgt gttatgtatt tcaacactta
     4981 aacagcccac atttttgggc gtgtgttgaa atgagatttt gaactttgag gaggtacatc
     5041 atgacaaata ttaacaacct aaccgacgat gaattaatgc gtttatatga tgaggaaaac
     5101 aacaaagtta aagcactacg tgaggcgatg aaacaacgta agaagataca acataagata
     5161 gatgaatcag agcgtaaggt taagagtatt aatttccgag tgacacaaaa cgcttatgat
     5221 gaattatcac agatgtgtga agtgttaccg agttttaaat cacctaatga ggtggcaagg
     5281 tctgtgttaa tggagtggtt agataaagat aaaacatttc taccgcctcg caacacattt
     5341 gatcccgaga catttgagtt attacagaat ttatatcgtg tagcaagtga gttgcgtaat
     5401 gatatgagag gaatggctaa taattataat caacatgtta aacacctcaa taccttagta
     5461 aataaacgag agcttacacg tagtgatata gaagaaatta aagaattcaa tacgtttaga
     5521 aagacgttaa cgaaacaagt caataatttt aataaggagg tgggaaaatt atgcctactt
     5581 tacaagtaag tcgtacatac aacatacaaa agtctattga ttatgtaacg tctgaaaaag
     5641 cacattacaa ccatgagcaa acagagggta aacgtgttca atgggcgtcg ggtgttaatg
     5701 tacctataac aaataaaaca gcattcatgt ctaaaatgca catgcgtgaa gtacaaaaag
     5761 aatatggaaa agataaaggt tatgtacaag gctattctat cattcaaggg tttggtgctg
     5821 atgagataga tcctaaaggt ggtgtgaaca gtagaaataa agcgcatgat ttaggactac
     5881 agtttgcaca acaagtatat ggtgagaaca atcaagctat cgtagttaca cagtgtgatg
     5941 gtaaaagtgg attgtggcac aaccatatca tcagcaatgc ggttgggtta gatggaaaat
     6001 cacaaagagg aaatatgacg aagtggacgc aactagcaaa gattaatgat gaaatagcat
     6061 tagagaatgg acttacacca ttagacaaag ataactggca aaagaataaa gatagaaaac
     6121 gtgacgcaaa agctaaagga caatatcaaa caccaatcga aaagcgtatg cgtaaaaatg
     6181 gtgttgtctt agctaaagat gaagtaattg aacaaatcag aattgctaga gatgagccct
     6241 ctgttcaaaa cattgatgat ttcaaacaat tgctacatga agaatataac atcgatgtgc
     6301 ttgaggctag agctaaagac ggtgaaaact tacgctacag ttatgaatac actaataaaa
     6361 aaggtctaac caatacgaaa gtatcaagag catcaacatt agctggagaa gaattccaat
     6421 taacaccatt aaccaaagaa attgaaaaga aagttgcatt acaacaggcg gaaaaagaga
     6481 aagatcctgg tttggttgat tatggtttag atatagaaga agaattgaat acaccacaag
     6541 cacaaatgat gatacagaat ttacttcaaa aagacgaaga agaggaagaa gaagagcttg
     6601 aattggaatt cagtgaagaa gatttgcaag tattaaatga catagaaagc gaggttaaag
     6661 attatgccag agaaaaagaa cgtgacacaa cagttgttag aaacaataac cgaaatcaaa
     6721 aacagcattc agactatcaa cacaacaagt caagacacga aacaaataca acaagaacaa
     6781 aacaaactcg cagagctact aaaagcaaag acgacggatt tacacttgag taaagagaca
     6841 caacagaata tagatatatt aaaaaagaaa ttagagaatt tagctaatga gacacacgaa
     6901 gtcaaagtaa acttagagcg taatgatata tatgaaatag cagatgctgt ttcaaaaagc
     6961 ttagaaatac atttaaagac ccaattagag gacaaaatca aagaagatga gaatacactc
     7021 aaagacgcta caaggtacct taaaagacaa gctgatgaca ttaaaaagga aacggaggac
     7081 ttaaccaaat ataaaaaatg gtttattaca gcaatgattg gttttgtagc atttgcagtt
     7141 gtattactta tattcacaac attagtagat atgccgtttt tagaacatgc atatcaattt
     7201 atcgcaacaa aggtagaaca tagccacaaa tggtatacaa cagcgttgtg gtatattgca
     7261 tatcttataa caccactgtt ttggataggt gttgtgacag gtgtagcgta tctgttacac
     7321 aaatggtaca cttcaaaatg gaggtaatca tatgttaaca atcaaacaac tagctgataa
     7381 attaggtgta tcaaaaccga cgatcacaag aaacaaaccc gatgacttag aatttgaagt
     7441 tatcgacggt gtgaactaca tcaatgaaaa catggagcgc caaattaccg aaaaagtatt
     7501 gcaaaacaaa gagcgttaca gtggcgatac acgcaatgaa acgtacgata caccacttat
     7561 acaacagtta atgcaagaga acgcgtttct cagagctcaa attgaagaaa aagacgcgtt
     7621 gattaaggaa caaaccaaat tattagatca acaacagcaa ttaaacctca ttacaggtaa
     7681 aagattaaat gagttagaaa cgaaagaaca gaccgaaaca gaagaacaac atagtaatga
     7741 aacagttaca aaacacactg tttcagaggg gaaaaagggc ttttttagta gaatatttgg
     7801 aaattaaaaa tagcactgat gcgtttgata cgcaaaagtg ctataataca taaataaagg
     7861 tgacttgcta ccaacaagac accaaatgtt aaagatgaaa aaacagacgt caagaatctg
     7921 ttgtttatta aattgatata tttataatac catttacgta cgtaaaacac aattataaat
     7981 cacgcttaaa aaaaataaac tatcgtcttg ttggtagcac acctctaaat aatttagaaa
     8041 ggtgtgctta tttttatgat tacagaagaa aattatagaa gcaacggcat gaatattttc
     8101 ataagaagaa cgaatttaaa actcacatac aaaggtagac aagtgagttt taaaatcaac
     8161 aaaaaaggca tagtgacagg cgtttcgcat gggggtcatt accaaaccca tgggggattg
     8221 ccagggatga cccccttttt gactccgccc aattattttt taaatcctgc tttatttttt
     8281 agagttagtg gtttaataga ttattatgta aaaaatgata ttactccttt atgggttggt
     8341 gaaaatggat cttgtagtaa agttgttgaa ggtacttgga aatattttat taaaaaatat
     8401 aaagaatcta aaaaagtgat tggtaataat gggttaatga atcgtgttcc tagcggtttg
     8461 gttgatgtac gtgattttgt tcttagatat tgcaataaag tttatcttga ttttggtttt
     8521 gatactgaat ggtatcatga tgatcgttat attttatgtt atcaattttc tactacctta
     8581 gatgatatac aatatgattt tgtgtttcat gttgttgata aatgtcgtct ttctctaggt
     8641 gaaatgattt atcatattgt atctaataca tttgcgggtc gtggttttgt tattttaaat
     8701 gaaaaacttt tacatcacaa aactcctttt tatgaaagtt gtaatattag acttatagct
     8761 cattttgctg gtgttgattt ttctatgtgt aatgattata aagaaatttt aa
//
