LOCUS       CP052982               13569 bp    DNA     circular BCT 09-FEB-2021
DEFINITION  Staphylococcus epidermidis strain HD25-1 plasmid pHD25-1_1, complete
            sequence.
ACCESSION   CP052982
VERSION     CP052982.1
DBLINK      BioProject: PRJNA546129
            BioSample: SAMN14732892
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buttner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Distinct clonal lineages and within-host diversification shape
            invasive Staphylococcus epidermidis populations
  JOURNAL   PLoS Pathog 17 (2), e1009304 (2021)
   PUBMED   33544760
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13569)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buettner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 149x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2020 13:53:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,626
            CDSs (total)                      :: 2,545
            Genes (coding)                    :: 2,466
            CDSs (with protein)               :: 2,466
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 35 of 79
            Pseudo Genes (incomplete)         :: 38 of 79
            Pseudo Genes (internal stop)      :: 30 of 79
            Pseudo Genes (multiple problems)  :: 18 of 79
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="HD25-1"
                     /isolation_source="nose; prosthetic joint infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pHD25-1_1"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2018"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            1..936
                     /locus_tag="HJH96_13040"
     CDS             1..936
                     /locus_tag="HJH96_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QRJ31038.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(1161..1400)
                     /locus_tag="HJH96_13045"
     CDS             complement(1161..1400)
                     /locus_tag="HJH96_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="QRJ31039.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1417..1686)
                     /locus_tag="HJH96_13050"
     CDS             complement(1417..1686)
                     /locus_tag="HJH96_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ31040.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            1895..3817
                     /locus_tag="HJH96_13055"
     CDS             1895..3817
                     /locus_tag="HJH96_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QRJ31041.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            4091..4504
                     /locus_tag="HJH96_13060"
     CDS             4091..4504
                     /locus_tag="HJH96_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="QRJ31042.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            4581..4814
                     /locus_tag="HJH96_13065"
     CDS             4581..4814
                     /locus_tag="HJH96_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ31043.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(4863..5567)
                     /locus_tag="HJH96_13070"
     CDS             complement(4863..5567)
                     /locus_tag="HJH96_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ31044.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5718..6416)
                     /locus_tag="HJH96_13075"
     CDS             complement(5718..6416)
                     /locus_tag="HJH96_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="QRJ31045.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            6580..6678
                     /locus_tag="HJH96_13080"
     CDS             6580..6678
                     /locus_tag="HJH96_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QRJ31046.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6855..7767)
                     /locus_tag="HJH96_13085"
                     /pseudo=""
     CDS             complement(6855..7767)
                     /locus_tag="HJH96_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(8186..8746)
                     /locus_tag="HJH96_13090"
     CDS             complement(8186..8746)
                     /locus_tag="HJH96_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QRJ31052.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(8997..9563)
                     /gene="qacR"
                     /locus_tag="HJH96_13095"
     ARG             complement(9001..9563)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9741..11285
                     /locus_tag="HJH96_13100"
     CDS             9741..11285
                     /locus_tag="HJH96_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /protein_id="QRJ31048.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="quaternary ammonium compound efflux MFS transporter
                     QacA"
     gene            11490..12050
                     /locus_tag="HJH96_13105"
     CDS             11490..12050
                     /locus_tag="HJH96_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ31049.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            12126..12248
                     /locus_tag="HJH96_13110"
     CDS             12126..12248
                     /locus_tag="HJH96_13110"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="QRJ31050.1"
                     /translation="MAPYTNKGYAVKELCSYLQINMDDVYTIGDRKLIYLCLIP"
                     /name="HAD hydrolase family protein"
     gene            complement(12369..13130)
                     /locus_tag="HJH96_13115"
     CDS             complement(12369..13130)
                     /locus_tag="HJH96_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QRJ31051.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             9742..11282
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..936
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1896..3748
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atgcccaatt ttgaaaaata taatttatca caagtaaaaa ctgaaagatt ttatcaactg
       61 cctaaatatt tatttgaaga tgcatatttt aagaaaatgt ctgcagaagc caaaattatg
      121 tatgcgttat taaaagatcg ttttgaatta tccctccaaa atgaatgggt agataaaaat
      181 aataatattt actttatttt cagtaataaa catttgtgtg aatacttagg ttatgcagaa
      241 caaaaaatta taaaattaaa aaaagagtta ataaaattta atttactaac tcaagaacgt
      301 gttggcctta ataaaccaaa tagattatac ctattaaaac ctaattatga cattgaagcc
      361 agtcatatca aggaacttcc aaattcacag ttccagaaca atgaatttgg aagttctaga
      421 actgtgaatt taagtggtca agaacttcca aattcacagt ctaatgatac tgattataat
      481 gacactgatt atattaagac taattataat gatatgtatg atttgaatga taggaaatta
      541 acatattcta gtaatcatac aaatcattca aatcactact attcaaaatt caatgatgaa
      601 gctttaaaat ttcaattact tgaagaacta ccacaaagta ttcaaagtta tttaagtaac
      661 ttttctgtat ctgaaattaa acttattaaa tctgtgttat taaaagccaa aacatccttc
      721 aacaattcta ttgatgcata ttatttatta gaagatatgg aatttgaaat tgttaatgtt
      781 cttaagcgtt ttaaagctac attaattcaa aaaaatgaaa ccgttgaagc aatgcaaggg
      841 tacttaatga aatctcttaa atctgaattt gcagaagtac atacgcttaa taaacgacgt
      901 gatcatttac caatcacttc tttatttaat caataataca acttaataag ccaacaatga
      961 tccaattaga gaatatcttt aattagatgt tctcttaact gtaagcgaac tttattttca
     1021 atgaaatgta attaattaag taagtgggac taaaactttt cttttttagt cccattttta
     1081 taaacaatta ttattcattg ttactactat cagcaatttt ttctttttag tgaaacccaa
     1141 gtatttgtaa gcatgataaa ttacttcttt ttatatttat tgggtttatt tgtattaatg
     1201 tagttggaat acatctttaa caattcttct gtttcatcag gacttaaatg ctcacattca
     1261 aaatactttt caagcattgc accttttcga atcagtctat gtgctctttc ttttctatct
     1321 aaatttttta atctatgctt tcttaattct gctttttcaa tttcagcatt tatttttttg
     1381 agatgtttaa aatcattcat cgtatcactt actattctag ttaattttgt cttctgttaa
     1441 attttccttg ttctcgttgc tgctattttg ttcgatatct ttattaaatt ctccaatgac
     1501 tttatccata attttataca attcatcact agagttgatc atatttaaat caagtttatt
     1561 taaaaaataa ccaccaattt tattagatag ttgttcttct tgttttttct tttgctcttt
     1621 cagttgtgca atttgttttt ctaattgact taattttgta atatctgttt gtttaatttt
     1681 agacattaaa aaactccctt agtttatcta tataaagcat accataattc attgggtaag
     1741 tcgcacatcc atggtataat ataaccagtt cttaagacac tgcactgccc atttacatgc
     1801 aaattgaaaa ttggcataaa aatgggcaac catactggtt gatcattata gttcccacag
     1861 gggtaaaaaa acagaaaaga gaggaggtaa aatcatgaat aaaggttact ttatgttgag
     1921 agtgactaac attaatcgag caacaaaaaa tatcgttgcg tcagcgtctt atcgttctga
     1981 tgaagcatta tatagcgaac gtacagatga aaaaattaag tttagaaatc atactattaa
     2041 gcctgaaagc atgattttaa ctcctcaaca tgcacccgaa tggactaaag atagacagcg
     2101 tttatggaat gaagttgata aagttgaaaa gaataatgcg aaaacaaaaa atcctagatt
     2161 agcaaaagaa gtcttattaa gtttgcctaa tgattttgat agagaggtac aaactgaatt
     2221 aactaaagat tttataaaaa gtgagtttgt agacaaagga atggttgctg atatttcaat
     2281 tcatagagat gatataaaca acccacacgc ccatgttcta cttactcaaa gaccgtttaa
     2341 tgcagacggt acttggggga aaaagacaaa aacgagaacg caatatgatg aaaatggcaa
     2401 tgcgatttta aataaaaatg gtaataaagt tagaaaacaa gaacgatttg ccgactttga
     2461 tttcaaagaa gttcgtaagc attgggaatt aaaattaaat caatactcag aacgtgaaca
     2521 aagtttaaga cgatatgata gtcgttcttt tgaagcgcaa ggtttagaaa aaattgccga
     2581 aattccactt acaagagaag aatatagatt agaacaaaat gaaagaaaac gctgtgaaaa
     2641 agagggtatc gagtaccaac ccgttactta ttacggacag aaaaatgaac aaattagaag
     2701 atataatcgt ggcgaagtat cacaattatt tagtgataaa gagaaagaac aagcacaaaa
     2761 tgaaattaga ttatttattc aaaatgctaa cgaacaagta agtaaaaatg aacaaagtta
     2821 ttctatttta tttgatagat atcaacgtga tgttggctat tcagaggcga aagaaacaat
     2881 taaaaatacg tttgacagtg cgtctacctt tggtcgtaaa attcaaaatg atttacttaa
     2941 aaatgaaatt aagagacgtc aattaaaatt ctatgctaaa aattatgatg aaaattatca
     3001 gtttagaaaa gaaattgaaa gtaaaggata tagtcaacaa gagtttgtta atactgttga
     3061 aactaactat aaagcgttac tcaatgagaa gaataaatta gaaagtagag aggaaaaacg
     3121 taaagagatt tatcaatcag ctaaagatgt ttatctaact gaaagtgaaa aaaatcatct
     3181 cattgttcag caaatgtacc ctaaatctca tcatcatttt agccatgatg aaaaagcatt
     3241 tattgttgat gaggcacaaa aaggtaatat tataaaagca actcatgttc aatacctgtt
     3301 caaacaaaat aacaatattc ctaatgatgt attgctagaa gatacttata aaaaagcgag
     3361 taaagatatt ttctttagtc aaaaacatct taaaaattta gaaaaagata gctttgaata
     3421 taacgttgag ttgcaattta ttaaggaaaa agaaaaagaa attgatagtt ataaagaaca
     3481 tattgatgat gatttaagaa tagatatagg cgataaaaac tttgaaaaag tcaaagacca
     3541 aacacctaat gaaaaagttc aattactcat taaacttgag aaatataatc atgtcgatca
     3601 agaagaagtt atcgaaaaac atattaatca agtagataga aatgatcatg acaatcgtat
     3661 tgaacatgaa aatgaacaaa ctaatcacat taatttacca acgataaatg ctagtgactt
     3721 tcttatgtac tatgcgaacg aaactgaaaa gcaaaacaat attaatcatc agaaaaagaa
     3781 gaagaaacaa gagagaaata accaaatgag aagatagtaa gcagttgaaa tcatacaaag
     3841 ttcattcttt aataataaga aacttaaccc aatgtggtaa attagctttg gtttaaagaa
     3901 aacatttata tacttagata aggtacattc tatgtattat cacaatgtat tttaaaacgt
     3961 gtataattat agaaaaacct tgatattatg cgattgttca catatttcag cataaaaaat
     4021 actaatgttc ataaatatcg atagaaaaaa ttgagtttaa atatatctta aagttaagga
     4081 gaaaaataat ttggaaacaa aaattggaca tcaaatacaa aagttaagaa aaagtaaaaa
     4141 catgtctcaa gaaaaattag ctgaaaagtt aggggtatcg agacattcta tttctaattg
     4201 ggaaagagaa gtaagtaatc ccgacttaaa aacaatttta gaaatcacta aattatttaa
     4261 tgtttctcta aatcaattaa ttaaaggagt ggaaatcatg caagttaata aatatgttta
     4321 ctctgcatta gcattttttc taggaggttt cggggctcat aagttttatg ttaagaaaaa
     4381 taaaaatgct cttctttatt tactattttg ttggacggga ataccgggtg tgataggtat
     4441 ggtagaaggt gttttaacac ttatgcgtcc gagcgatagt gaaaacaata tagaaataaa
     4501 ttaaaccaaa aataaaaagc cgatgtatcg gatttttttt agtgtacatg tgaatattaa
     4561 aaatgatgga gtgatttcac atgaacacat tcgttttcgg cgtgattaca aagcgattag
     4621 aagaaatagg ttttaatgta cacaaggtgt ttaaaatgcc cgaaaaacag gcactatttg
     4681 aagatttgta ccattatgtg agatacgtta aaccatgcaa ggctgaacta acaatggtgg
     4741 cattagatag tgatgaattg attcattact ttatatgctg ttacatggaa cgttatgctt
     4801 atcttttgaa ctaatgataa aaaagacgcc taattttagg cgccttttta ataatctact
     4861 aactacttat tatatctaat tttacttgct aacttagcta ctgttttatt gtcgtagggt
     4921 ttaatataga ctgtttcaat attctcctct attgcttttt ttgttatctt attcatttga
     4981 ctataatcac tattttgtga attttcccat ttataaggga tattattata tacattaatt
     5041 gttccatcac cattactgct atatgttaca gacccaccag ctctcatagg agcttctaat
     5101 ttcacaactt tttctttata aattgcacta tttttggctt gtggatttac ttttgagcct
     5161 tttggtattt ctgtaacata aacattttta atacctttta aatcatttct tgtagatatt
     5221 aactgatacc aaactcgtgc atattcaatt tctttcgagt taaaattatt taagtaactt
     5281 ttatttttat cagtagattg ttgcttctta ttactatcat tatttttttc ttgtttttta
     5341 ttatcatttt tttctttatt ttgtttatta ctactttctg aattatccgc actatcagat
     5401 gtgttagttt tttcgtcaat ttcatttgat gcttcattat ctttgttttc tgaggtgtta
     5461 gtatcactag aattattatc attgtgatgt tctgaatcgt tattattaca actcactaaa
     5521 attaccgata aactaacaat taatataggt aaaatgattt ttttcatatt acaactcctt
     5581 ttcagtattt caaattagat tatgttaata ttttttcagc aagaaaacgt aaattacatg
     5641 agttgaaaaa tatgtattaa atatataata aaataaccac atgttttaca taaacacggt
     5701 ggttatttta tcaattttta tattgccttt tttctgaata ttaaaagact tatacctaat
     5761 attagtagtc cagaagcgcc aattattgac aatatatcac tttggttgct ttctcctgtt
     5821 tctggtaata cttgagagtt tgaatcttga tttttcattc cttcagaact cttgttttta
     5881 ttttcagtta caggtgtcaa cacaccttga ggtgtattaa tagttttatt ttcttcatta
     5941 gctatctttt gactttcatt ttttacaatt tgattatatt tttctgtgga tacactatta
     6001 gggttaggaa catgactttc taattcttgg tattcttctt gagtcataca attaggtatc
     6061 acccctccga aaggtctttt accttgttga gttctctcat tattttgtat atcagtactt
     6121 tgtgattgat attcattaaa tacacttgaa actttctcat tatactcgat ttgagttaat
     6181 tcccctgaat ctttctgttt agaaagttca cttatttgat tttgtgcaga ttgtgggagg
     6241 gatgaagtat tgaagtctac attagcccaa ccatctccat ttgtatctgc gctatttggt
     6301 aagttctctc cgtatacggt tttggcacca tcctcagcat tggctgtcgc tgaaaaacct
     6361 gtgaatgtta atgagctagc caataataaa gttggaagta atgatcgttt tttcataaaa
     6421 ttatctcctt taatagaatt tgtattttca ttatcacata gatacattaa acataaaatg
     6481 acttatcgga agtgcatatg cttaacaatt cacaaatgat ataatataaa agtcgtcgtt
     6541 ctacttctgt gctggtttag catggaagga ggtgacacca tgttcagcat catatttgta
     6601 tctatagtag ctcctattat agtaggggtt ataatcacgt tgttttcaca ttggttgaat
     6661 aatcgtgata aacaataaag cgacgacaca gtaataacac caaaaagagc ccgataacta
     6721 ctggaatagt tatcgggctc ggtgtttacc atgttcaaca catatttgtt atctaaatta
     6781 taacatcata gtgtggagga atgcaaaatt gaataattag tatgttaagt ctatactata
     6841 ctgattagta agtactaatt atcatctttg ttattgttac ccctttcatt ggcttttgaa
     6901 tctaattctt tttgaaattt tttgtgtgat tttgaagaag tgtttaaaaa gaaataggtc
     6961 atattatcca tacgatgtcc ttttataaat tcattgataa attgtttttt ttgtcatttt
     7021 tttggttgta ttacgttgtt tttgcaatct aatgatttct tcttcaatgt atactcttat
     7081 catcaaatct aaactagatg tctgattata agtagtgaat aattttggaa ctaataaagt
     7141 tttattacca tcaaaaagaa atggagtgaa atcataagtt tcccatatac cgttagtaga
     7201 gaaataataa tatgtcacag tttctttaga aagaaacgat ttcatttctg gaaatgactg
     7261 aatcatggat ttattaaaat caattataac attcatgatt atattacttg ttaaatctga
     7321 taatttatca tcaccaaaat ttctgattaa tggtgctttt tgaggtattg ttaatccgtc
     7381 atcatttaag ataatggaaa gaatatcttc attcataaat agattaatca attcagatgc
     7441 tgtagtacct tttcctgtat tacctttctg agaaaaacct aagcgtaaat ggtgtggttc
     7501 tgaaaaatga gagaataatt ttctaaattc attacgcttt tgggtatcag aaaaatcact
     7561 aagatgtata tcgataattt tttcaaaaaa aacatcaatt tgctcttgtg ctctttgagc
     7621 caaagtcgca acttttccaa cttctctatt agatattact ttaataaggt ttgggtctat
     7681 atattgaaga gtgtcttctt caatagaaat atttaaataa tcagttgatt tagcatcatg
     7741 attgaatttg gtacatacta gtgtcactct atatcatcct tctgtaagtt ttttgtttac
     7801 ttccaatata gaatgatgta ttattgtaat tgtcgattta caataagttg ttattctagg
     7861 tggaagttta ttgcttttga tgtatagata gacttaacct tttcattata gcatactata
     7921 gaagaaaatg caagcaaaag catataaaca acaaatgttg tttatatgct tttgcttttt
     7981 tgttaattgg agaggacaag gtgaatatta acagtcaaaa tccagttatg tcggttaatg
     8041 aatcgatgaa aattttcaaa gctcaagtaa tgcttagaaa tttaaagcta ttaagcgagt
     8101 ggattgttaa aggggactga agttaaagac actttttaag aagatttttc aattaaaggg
     8161 tatattaaaa ctttaattta gtactttata tttttcttga ctctttaata atcctatata
     8221 cagtagaacg actcactcct gtttgttcag caatttcttc accagacagc ttttgttcat
     8281 catataagaa tttgatctct cttttcttat gatctggtag tgaaggtctt ccaccttttc
     8341 tccctcttgc tcttgcagct tccaaacctt ttttagttcg ttcacttaat aaattagctt
     8401 caagttcagc gaatgcactc atcatggtaa agaacatctt gcccatcgca tctttcgttg
     8461 acacattcat atctataata tgtaaatcaa tgctattttc atccaaccat tgagatagct
     8521 caattaattg ttttgttgta cgtccaagac gatctaattt atagacaact agaatatctc
     8581 cttcacgtaa ataatctaaa cacttatcaa gttccgttct tttagtttta cgaccactcg
     8641 ctttctcgct aaagatacgt tcacaaccat attcctcaag tgtatcaatc tgtccatcaa
     8701 gactttgatc ttgtgttgac acacgtgcat aaccaatttt agccatttga acaccacctt
     8761 ttttaatcgt tttatgggta cttactaatg aaatatagtt ctcgatgtaa ataaacacgt
     8821 ctgtcagcat ctaaaaactc atgattttcg atatttatga tacattgatt ataacacaag
     8881 tttatagact taaatatcca agaaaaacct cttaatatta agttaagagg ttttgtgtca
     8941 aaaacgaacg taatttagaa ataatattta ttggtatttc aagaatatat atataattat
     9001 ttactaagtc catttaaaaa aatttgagag aacttgttca taagtttaat tctttcatta
     9061 atattttgtt catgtgtaaa ggtaacaatt ccattaacag cgtttgctgc tattttacta
     9121 acagcattaa catcattaat acaccattcg ccatttaaat taccctcctt aaaaattaca
     9181 tgataagcat ctatatattt gttttctaat ttattcattt tttcattaat gctattagtt
     9241 ttataatatt cagtgtaaaa ttcaattatt gcgttttgaa gtggataata gtattcggtt
     9301 gttaaagata gttcattata taaataaaat ttttctctat tagttttaca tttgatttgt
     9361 tcctttttcc actgttcttg ccatttagat tcttctatat ttaaaatttc taaaaataga
     9421 ttttcttttg ttttaaagtg ataataaaga ttccctttac tactttctga taatttaaca
     9481 atttctccag tagtagtggc attatatcca ttttttataa ataattcctt tgcgacacct
     9541 agtattttat ctttcaagtt catcacgcct ttaatgagta ttaagtacta tcaatgatag
     9601 cacaaaaaat tcaaagaata attgtataac acaatacagc catttaaatt tcgcaagatt
     9661 ttttgtggta atatgtaaaa aaatagatta taatccttat agaccgatcg cacggtctat
     9721 aaggattgga gggaacttaa atgatttcat tttttacaaa aactactgat atgatgacat
     9781 caaaaaaaag atggactgca ctagtagtat tagctgttag tttgtttgtt gttacaatgg
     9841 atatgacaat attaattatg gctttaccgg aattagtaag agagttagag ccttctggta
     9901 cccaacagtt atggatagtt gatatatact ctcttgtttt agctggcttt ataattccat
     9961 tgagtgcctt tgctgataaa tggggaagaa aaaaagcatt attaactgga tttgctttat
    10021 ttggcctcgt ttcattagct atatttttcg cagaaagtgc agagttcgta atagctattc
    10081 gatttttact tggtattgca ggtgctttaa taatgccaac tactctttca atgataagag
    10141 taatttttga aaaccctaaa gaaagggcca ctgcattagc tgtatggtca atcgcttcat
    10201 cgataggtgc tgtttttgga ccaattatcg gaggagcttt acttgagcaa ttttcatggc
    10261 actcggcatt tttaattaat gtaccgtttg cgataatagc agttgtagca ggtttatttt
    10321 tattaccaga gtctaagtta tcaaaagaaa agtctcactc gtgggatatt ccttctacaa
    10381 ttttatcaat tgcaggcatg attggactgg tatggagtat caaagaattt tcaaaagaag
    10441 gactagcaga tattattcca tgggttgtaa tagtattagc aattaccatg atagtgatat
    10501 ttgttaaacg taatttatca agttctgatc caatgttaga cgtaagactt tttaaaaaga
    10561 gatcattttc agctggtaca attgctgcat ttatgacaat gtttgcaatg gcatctgttt
    10621 tgttattagc ttcacaatgg ttacaggttg tggaagaact ttctcctttt aaagctggct
    10681 tatacctatt acctatggca ataggagata tggtgtttgc accaattgca cccggattag
    10741 cggcgcgatt tggaccgaaa atagtgttac cttccggaat tggaattgca gccattggca
    10801 tgtttattat gtatttcttt ggtcatccat tatcatattc tacaatggct ttagcattaa
    10861 ttttagttgg agctggtatg gcttcactag cagttgcatc tgctctaata atgttagaaa
    10921 cacctacatc aaaagcaggt aatgcagctg ctgttgaaga gtctatgtat gaccttggaa
    10981 atgtttttgg tgtagcagta cttggtagcc tatcttctat gctttatcgt gtatttttag
    11041 atatttcatc tttttcatca aaaggtatag ttggagattt agctcatgta gctgaagaat
    11101 ctgtagtggg cgctgtcgaa gtagctaaag ctacggggat aaaacagctt gcaaacgagg
    11161 ctgtaacatc atttaatgat gcttttgtag caactgcttt agtaggtggg attatcatga
    11221 ttatcatttc aatagttgtc tatttgttaa ttcccaaatc acttgatata actaaacaaa
    11281 aatagtacta aacatatatt tttagatatt gtagattagt ttatttttaa aattagtaat
    11341 agttaaaaag tagaaccatt aattttaaat ggttctactt ttttattatt aagtaaaatt
    11401 ataaaagaca atttcttaaa ttaataatat gatataccta tatctaataa tttattagta
    11461 ttaataaacc ttgaaaggat gattaactga tgaatagagc agatattttt aaaatatttg
    11521 atgtagaact taatataact atatttttag gagcactagt agcaactttc atctatttta
    11581 taagttacat gaacaatgta aatgaattaa ttacattatg cttaacatta gcttttcctg
    11641 catatttatt aaaaattagc agatcgttcg aggttatttt attagaggaa aaaataaaaa
    11701 atagtaaaat caaaatctgt gacaaagata gaattgaacg agagatactg gtgaaaaaga
    11761 acctaataga tgaactattg tttcctaata gagttattaa aaaatataca ctgacaattt
    11821 taatttacag tataggtatt attattcttt tactactttg taatatgtta agttatggaa
    11881 ttggtgaaaa tgtcaatttt atctggagat tactagcttg ttatgtaacg agtgtcacag
    11941 tgattatagt tgccttgatt ataatgataa tgatatttga tcttataatt ggattactat
    12001 taaattttca aatattttta aaaaataaaa agtatgatga ctttttataa ttattcgatt
    12061 tatataatga aaaattaaat gattaaacat agaaacctca aaacaaacca tatttaatag
    12121 agtttatggc gccttataca aataaaggct atgctgttaa agaattatgt agctatcttc
    12181 agattaatat ggatgatgtt tatactattg gcgatagaaa attgatttac ctatgtttaa
    12241 taccctagat aattcttctg ataatttagt ttttgttaga gtttaacata actgatgatt
    12301 atcagaagtt tgattcacat taaaaaggca caacaagttc atgaacttgt gtgccttttt
    12361 aattgcaatt agttaccaaa taaacgactc caaaatctct ttttaggtgg ttctacctcc
    12421 ctattatcac tggaatcgtt cttcgttttt tcaacaaact tatcctcttt tgttaattca
    12481 tctgtactgt catgcttatt ctcttcagtt atatttttat gatttacttc ggttggttga
    12541 cattcttttt gttgttggtt gatattttga gattcttcag taaaagttgc ttcttgtgga
    12601 ttaatattct gtctattatt catcgttgta tcaaaagaat atttaagctg tctttcttct
    12661 tctaattggc tttctagttt ttgaattttt ttattgtttt ctaaagataa tatttgttga
    12721 ttctccaaca gtttatttaa tgtattaaca ttgctatttt gttgatctaa ttgtttagtt
    12781 agattctcaa tatatttttc atcattttta gctctagttt caaagatttc tatttgcttt
    12841 tctaattcgt ttacttttgt ttttaatgtc tcagaactat ttgaattatt atgtatatac
    12901 ttttcaactt ttgcagtatt ttcattaaat aattcatttt ttactgattc tttattatga
    12961 gttgttttct ctttcttagt gattttttct ataattttta aataatcatt atctttaata
    13021 taattaattc cattttcttt ttcaaaagat atatttaatc ttttagcatt attaacaata
    13081 gtttgtttag ttacacctaa ttcatcagca atcattttaa tagttttcat aacatccccc
    13141 ttaggtacct aatttagtac ctaaatttta ccacctaaca aaatttattt catcatttag
    13201 tactaaattt actatctatt aactacctaa atgcctaatt atctatcctt aaaatatttt
    13261 cgccacttaa ttaggtgcta aaatagcgaa ttatacgttt ggtagtttta ggtgtacttt
    13321 taattacatt taaaactctt tatatacgcc attaaaagtg ttaatattac tttataaata
    13381 ttaaaagagt cgatgctatt ggcgtagcat cgactctcgg taataaaacg attcgcactc
    13441 gtttgtttat atattttttt gatacttgta ttatatatat ctaatcatct aagtgcaagc
    13501 acaaaacata taacttacgt aaaaattgtt ttattacctc aatcccaaaa tggaaatgag
    13561 gtttttatt
//
