LOCUS       CP052980               10645 bp    DNA     circular BCT 09-FEB-2021
DEFINITION  Staphylococcus epidermidis strain HD26-2 plasmid pHD26-2_2, complete
            sequence.
ACCESSION   CP052980
VERSION     CP052980.1
DBLINK      BioProject: PRJNA546129
            BioSample: SAMN14732893
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buttner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Distinct clonal lineages and within-host diversification shape
            invasive Staphylococcus epidermidis populations
  JOURNAL   PLoS Pathog 17 (2), e1009304 (2021)
   PUBMED   33544760
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buettner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 92x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2020 13:50:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,361
            CDSs (total)                      :: 2,280
            Genes (coding)                    :: 2,214
            CDSs (with protein)               :: 2,214
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 33 of 66
            Pseudo Genes (internal stop)      :: 20 of 66
            Pseudo Genes (multiple problems)  :: 16 of 66
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="HD26-2"
                     /isolation_source="nose; prosthetic joint infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pHD26-2_2"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2018"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(join(10485..10645,1..184))
                     /locus_tag="HJH97_11740"
     CDS             complement(join(10485..10645,1..184))
                     /locus_tag="HJH97_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28577.1"
                     /translation="MQKENIKKAQNEIPEEHLAVLQEIKNAPNKYITKEKLLIKLEMPK
                     SYWRTLNSIISDLIIKYGYIIGSTRHNGYYLCESDEDIDKAIYTLESLNGGIFRRIEVL
                     RNAKENKERA"
                     /name="hypothetical protein"
     gene            complement(640..783)
                     /locus_tag="HJH97_11745"
     CDS             complement(640..783)
                     /locus_tag="HJH97_11745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28578.1"
                     /translation="MRRQENDELTKTSFLIISVLVFKLLISWNDFLTSVFNFLSELTKM
                     MQ"
                     /name="hypothetical protein"
     gene            complement(934..1350)
                     /locus_tag="HJH97_11750"
     CDS             complement(934..1350)
                     /locus_tag="HJH97_11750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28579.1"
                     /translation="MNNIDDVINNMFDDKKEVTNNNQSKLRQSVKKVIDNESKNIKLDF
                     NNTFQNNNSVQSNKDNKRIQEAQRTHVEARQIRKDMQETKRITAEQRTAILKALNSGNT
                     PLHEVFEMCIDCIALTVNDKAFKRQAIKSYKENY"
                     /name="hypothetical protein"
     gene            complement(1365..3410)
                     /locus_tag="HJH97_11755"
     CDS             complement(1365..3410)
                     /locus_tag="HJH97_11755"
                     /inference="COORDINATES: protein motif:HMM:NF015736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28580.1"
                     /translation="MALYKDDEIKRINFEAIPHEMKIIPQWFLWKAKDTGRTDGKLAKY
                     PTDINGNTISWNNTDNLYMFDEVKKVYESSNEFAGISFNVVGSGLTVIDIDMNVNTETG
                     EITEIKPTEKPFLNAGYVEHSVSGRGYHVVLQGDLPSELAQEKNVYDKDGDKLEVFHSS
                     GYIAMTGDVYNNHSQIDNKEISKQFIKHLSQNYRPKGHTFESHSEAHTEPKQLSDSIKP
                     PKVNTIDVLRLWFNTGGKKLKTKNVSKRDLWERSYEAMQAYNNDRSEASYAIISELAYF
                     MWDNPQGLYDIVINNTYWNDPDNYMKDNAKRLQHDIKKAISERKASGHLYQAPASNKDT
                     VTETTEAEQRQNELLEHYNKSNAGAGLVDFINGIKENANTPPTSTGFPILDHVLNGGIR
                     EGLYVIGAISSLGKTTLVQHIADNIAQNDTDVLFISLEMARTELMSKSISRETYLDTIE
                     YGGNIRNAKVSLDITDGSKHENFNNDEKKLIQTAIKRYSEYADNIYIVEAFGQIGHEDV
                     RQLVELHTQHTGRSPVVVIDYLQIMKPADPRQTDKTNTDVAVNSLKRLSRDYKTPVLAI
                     SSLNRESYNRPMSLSAFKESGAIEYSSDVLIGLDFTKMLDDPTNMDFEEEKDKTPRNIT
                     LTILKNRNGRTGKRINYLYDPRFNHFDEDEYYTKQTKKEVKKAKRG"
                     /name="hypothetical protein"
     gene            complement(4038..4427)
                     /locus_tag="HJH97_11760"
     CDS             complement(4038..4427)
                     /locus_tag="HJH97_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="QRJ28581.1"
                     /translation="MTKNDEEIKDVGTYQHLGIGRRLKHVKENDLAHGEFGKWLEKVKL
                     TNQQAHRFMKVSQEYENSNLTTSLNLGLNVLYQIATLPEPERTKEHTTSKGAKLVKTCH
                     FNTHIERIRTEIGIYSVRFRNVSRT"
                     /name="DUF3102 domain-containing protein"
     gene            complement(5126..6148)
                     /locus_tag="HJH97_11765"
     CDS             complement(5126..6148)
                     /locus_tag="HJH97_11765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28582.1"
                     /translation="MTNKNNKFNYRPNATSIKNATPEDLKSIELMEYQDQALATFHVVD
                     NSPLSHALKRTTATHEQLSFFSPDPPNTRKLDSGEFIITDPNIDEIRIDPQDEIVLRGV
                     SNLYEKEIEDKNIVRCRISSLIREAYGYPAKQKIRNKEYKDFMQRLDKLSAIRVKIQFA
                     EEGSGIIDAETNHIMNSIRRPIIEFIELTSEGEPSSKDWIVFKSKPIMYDYSKHKKYIE
                     HVPKTVLQAPKGVVNNNNYRILVTELYRRINQYKRALNSKGKIKYIDNMQKINYNKLFE
                     ECDMKNITPTQKTRHKKTINLIMDKFVNDDHIIKDYFEYDSKGTTQGRPVGIKFSLNEE
                     "
                     /name="hypothetical protein"
     gene            complement(6129..6989)
                     /locus_tag="HJH97_11770"
     CDS             complement(6129..6989)
                     /locus_tag="HJH97_11770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28583.1"
                     /translation="MREYDLQNDLLIPYENGTLTTVSNSVGAIIKGIEDKKNADFLSYI
                     GAFIDTHFIYEFSSFFETPLYQELNEKSIVNCIGELMTSNVEAGEQSEVKEEVYNIREL
                     YKMNEKIFDAITKRASDWLIRQKELKVYIYDCMIEVYDYLLNEKTFRQSYVKSFFIDKL
                     QSAEAKYLESELDLITLKATAKMYPMYEMQAFIDKNKIDIKDIFSKWNASNDMMKTYYN
                     VNIGYFETKDLNIANKLYSRKLKQLKTRQKFAKNIDGYSGEFLSSKVLDKAYFVVGEAN
                     DKQKQ"
                     /name="hypothetical protein"
     gene            complement(7106..7345)
                     /locus_tag="HJH97_11775"
     CDS             complement(7106..7345)
                     /locus_tag="HJH97_11775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28584.1"
                     /translation="MNINVEQAVKHAIHNSQNIPVGTYKFTEFLNHTGHSLYLNNDEIK
                     MAERDFYNAVQVGLVFNVEFVSKIDDISSLYKKY"
                     /name="hypothetical protein"
     gene            complement(7644..7961)
                     /locus_tag="HJH97_11780"
     CDS             complement(7644..7961)
                     /locus_tag="HJH97_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="QRJ28585.1"
                     /translation="MNWEIKNIMSDMEIVKEKIDDVMTSFTWFEEEYFTHEPNHVLNKN
                     EILAHGYRYHEHRIKNTQTIDLMCMYLREFDNLLDKFKKIDKENDRAHDEIGNGQHNDE
                     Q"
                     /name="DUF1474 family protein"
     gene            complement(7978..8262)
                     /locus_tag="HJH97_11785"
     CDS             complement(7978..8262)
                     /locus_tag="HJH97_11785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28586.1"
                     /translation="MDNKETWKLMQEHWELVGLIQANANTLNYIDEVREEFDGVMRESA
                     SLTRLETRLINILKDDIKKSQKTSKQLDEIAGVKTSNVLNFEKSKMLKK"
                     /name="hypothetical protein"
     gene            complement(8276..8440)
                     /locus_tag="HJH97_11790"
     CDS             complement(8276..8440)
                     /locus_tag="HJH97_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QRJ28587.1"
                     /translation="MAIKTYTVDEVATQLDISPRTVREYIRKGKLKAVKVGNKYIISED
                     NYRDFVNGK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8493..9410)
                     /locus_tag="HJH97_11795"
     CDS             complement(8493..9410)
                     /locus_tag="HJH97_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010689057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="QRJ28589.1"
                     /translation="MHNQNEIQHTQSVNTELLHRFLNYIDASEKTVQTYTRALKQFFKY
                     LYEHNITQPTRSDVLAFRDYLRDKGRKPTTIKSYIVSVRLFFQWTNQEGFYPNIADKIK
                     GAKLDQAHKKDYLTTEQIKDVLNNIDISTVTGARDYAIISLMVTGGLRTIEVSRADMED
                     IRNVGDSPALYIQGKGREEKTEYVKLPLQVFKAIGRYREMLDKQHEVLFTSTSNNNKGQ
                     RLTTRSISGIVKKRLKEAGYTSDRLTAHSLRHTAGTLNLMNGGSLEETQQLLRHSNINT
                     TMIYLHHLERSKNQSEKRIADALF"
                     /name="site-specific integrase"
     gene            9705..10307
                     /locus_tag="HJH97_11800"
     CDS             9705..10307
                     /locus_tag="HJH97_11800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ28588.1"
                     /translation="MTNSVGFITTKNASKLLGVSASSLRVYAQHMEAVGYSFKKIENAR
                     QFSKHDLQIISEALERYKLTGGAIKDALHYVIVKEEKGEEEAEALQSLPKEQRSQDVDR
                     LDVEQFKQELSKYISNNINSTLEKHFQNVTEAITDVSNTQDDTKPMQEEIERLRALNEQ
                     LQDERDRYKSENLNMSNEIEQLKSMSIWEFRKWKKDK"
                     /name="hypothetical protein"
ORIGIN
        1 atttgataat gaggtcgcta ataatactat tgagcgtacg ccaataactt tttggcattt
       61 ctaattttat taatagtttt tctttggtaa tatatttatt aggcgcattt ttgatttcct
      121 gtaacaccgc taaatgttct tcgggaattt cattttgtgc cttttttata ttctcttttt
      181 gcataatttt caccccactt tctataccct tattataaca tattacaata cataaaaaca
      241 ggcgataaaa ggcgacataa cgcgtatttc gggggtcttt tttcgtgaca tttattaaga
      301 attttactta tatagtaatg tgtattttat atattttttt gttgtttata tcagtatata
      361 tgttattata tatttactga tatgaacaaa cgtttcattg gtgcaatcca acggatctag
      421 tcaatccgaa atatgatcta gctattatga attagtcact cgtaatagca aaaagctcgc
      481 ctagttctct tctcgttcta ggtggctttt tttatgcaca aaaatacccc tcacacttat
      541 atatcttgat attgcagtct tgcactatac gttgcaatca gtacaaatcg tcgtacccga
      601 tatttaaagc gtttggggcc aatacttatt taattatgat tattgcatca ttttagtaag
      661 ctctgataaa aagttaaata cactagttag aaagtcattc caacttataa gtaacttaaa
      721 taccagtact gatatgatga gaaatgacgt tttagtcagt tcgtcattct cttgtcgtct
      781 catcggtgca accctcctta acaagacaac gcataggctt gctacacaag acatatatat
      841 taatatagca taactattaa ttagcaaaat ataggtatta cataatataa ataaaaaggg
      901 gttgaaattc agccccttaa tagcatattt actttaataa ttttctttat aactctttat
      961 tgcttgtcgt ttaaatgctt tatcattcac agttagcgca atgcaatcaa tgcacatctc
     1021 aaagacttca tgaagcggag tattaccact attcaacgct ttcaatatag ctgtacgttg
     1081 ttcggctgta atgcgtttgg tctcttgcat atctttgcgt atttgtctcg cttctacatg
     1141 tgttcgttga gcttcttgta ttcgtttatt atctttgttt gattgaacag aattattatt
     1201 ctgaaatgta ttattgaaat ctaatttaat attcttactt tcgttatcaa taactttttt
     1261 aacagattgt cgtaactttg attgattgtt attagtgact tctttcttat catcaaacat
     1321 attattaatt acatcatcaa tattattcat acaacgccac acctttaacc tcttttagct
     1381 tttttaactt cttttttagt ttgcttcgta taatattcat cttcatcaaa atgattaaat
     1441 ctcggatcat ataaataatt aattcgttta ccagttctac cgtttctatt ttttaatatc
     1501 gtcaatgtta tgtttcttgg tgtcttgtct ttttcttctt caaagtccat gtttgttgga
     1561 tcatctaaca tcttagtaaa gtctaatcct attagtacat cgcttgagta ttctattgct
     1621 ccgctttctt taaacgcact caatgacata ggtcggttgt aactctcacg attgagggag
     1681 gatatggcta acactggtgt tttatagtca cgacttaacc gcttcaatga attcacggct
     1741 acatcggtat ttgttttgtc ggtctgtcta gggtctgcgg gtttcataat ttgtaagtag
     1801 tcgataacta caacgggtga gcgtcctgtg tgttgtgtat gcagttctac aagttgcctt
     1861 acatcttcat gaccgatttg cccaaaggct tcaacaatat agatattgtc cgcgtattct
     1921 gaatagcgtt taattgctgt ttgtataagt ttcttctcat cattgttaaa gttttcgtgt
     1981 ttgcttccgt cagttatatc taatgatact ttagcatttc taatattccc gccatattcg
     2041 atagtatcaa gataagtttc acgcgatatt gatttactca ttaattcagt gcgtgccatt
     2101 tctaaactaa taaataatac gtctgtatcg ttttgagcaa tgttatcagc tatatgttgt
     2161 acgagcgttg ttttacctaa tgaagatata gcacctatca catataagcc ctcacgtatg
     2221 ccaccattta agacatgatc taatataggg aagcctgtag atgttggcgg ggtgttagca
     2281 ttttctttaa taccgttaat gaaatcaacc agcccagcgc ctgcattaga tttattatag
     2341 tgttctagta attcgttttg tcgttgttcc gcttctgttg tttcggttac tgtatcttta
     2401 tttgaagcgg gtgcttggta taaatgccca ctagctttac gttcactaat agctttctta
     2461 atatcatgtt gcaatcgttt agcgttgtct ttcatatagt tgtcgggatc attccaataa
     2521 gtgttattta taacaatatc gtacaagcct tgaggattgt cccacataaa ataagctagt
     2581 tccgatatga ttgcataact agcttctgaa cgatcgttgt tatatgcctg cattgcttca
     2641 taactacgtt cccacaaatc tcttttgcta acatttttag tttttaactt tttaccgcct
     2701 gtgttaaacc ataaacgcaa tacgtcgatt gtattcactt taggtggctt aatactatct
     2761 gaaagttgtt tcggctctgt atgggcttct gaatggctct caaaggtatg tcctttaggg
     2821 cggtaatttt gtgaaagatg tttaataaat tgctttgata tttctttgtt atcaatttgt
     2881 gaatgattat tataaacatc acccgtcata gcaatataac cactactatg aaagacttct
     2941 aacttatccc cgtctttgtc atatacattc ttttcttggg ctaattcgct aggtaaatca
     3001 ccctgtaaca caacgtgata ccctcgacca cttacagaat gttcaacata gcccgcatta
     3061 agaaaaggct tctctgttgg tttgatttca gttatttcac ccgtttcagt attcacgttc
     3121 atatctatgt ctataactgt taatccacta cctaccacat tgaatgagat ccctgcaaac
     3181 tcgttgctac tttcgtatac cttttttact tcatcaaaca tatataagtt atctgtattg
     3241 ttccatgaaa ttgtattacc gtttatatcc gtcgggtact tagctagttt gccgtctgtg
     3301 cgtcctgtat cttttgcttt ccataaaaac cattgcggaa tgattttcat ttcatgaggt
     3361 attgcctcaa aatttattct tttaatttcg tcgtctttat ataaagccat tgtgttgacc
     3421 tccctgttat tcaccaatca caaagttgtg tctctctcct gtgattgtat ggagttcccg
     3481 cccttgtgta aacaaagggg cggaacgact attatattta ttatatatat agaaaacgaa
     3541 atattggggt tgatacttac ggtaagtaag cgttagttaa gtttcgaaat tacacgtttg
     3601 tacgacccta attacacgtt tgtacgaccc taattacacg tttgtacgac ccccctcgat
     3661 aattttttga agcaaatttc cttttttatg gtgtccgaaa actccatttt ttataactca
     3721 ttaaaaaaat cgtctttttt atggcagtcg aaaaagctgt ttttcttttt tctgggtgcc
     3781 gaaaatgccg aatatcacaa aggggtctac aaaataggga taccctttaa tcactaacct
     3841 gtgtggtcga tatagctatg ataggttttg ctagaggggt aacgtttcgt tacgtcatag
     3901 ctcataattg caataatata agtacgcgct catatttgag cctgttaaat atatagctat
     3961 ttagttatat tactgaaacc atatttgggc tgagtataaa ccaatatccc ctattttata
     4021 ctaggttttg ctaggggtta tgtgcgtgaa acattacgaa atcttacgga gtagatacct
     4081 atctccgttc ttatacgctc aatgtgagtg ttgaaatgac aagttttgac aagtttagcg
     4141 ccttttgatg ttgtgtgttc tttggtgcgt tctggttcag gaagggttgc gatttgataa
     4201 agtacattta gccctaaatt taacgacgtc gttaaatttg agttttcata ttcttgacta
     4261 accttcataa atctatgagc ttgttgatta gttagtttta ctttttcaag ccatttacca
     4321 aactctccat gcgctaagtc attctctttt acgtgtttca atctgcgacc tattcctaaa
     4381 tgttggtatg tgccaacatc ttttatttct tcatcgtttt ttgtcataag ccccctactt
     4441 atccaacgaa ccttaacaat acttaacatt caatgtacgc ttaactacct aagaaaactt
     4501 aatatgaaat gcagtaataa gtagtatttt aatagtttta aatctcttga aaattaaagt
     4561 taattaaatg actatgttct tttttaaccc gtcgttaatg tcgtttcttt gcttaattat
     4621 atgtatttat tcaatgtaaa ttctgaaaga ttaaaaattg ctaaactcgt tgatataact
     4681 catattaaag aggatttaaa cctttcaaga attaacataa attagcataa aatagcggga
     4741 tcaaatctaa tgcaaaatct gtatattgct taatgaccca atcacaacgg agcgccccct
     4801 tatgtgaaag ttaaaactct ttgatatgtt gcattttgtt gcacctaata attgaaatct
     4861 ttcccacatt ttccaacaat ttcatattgg attacactca ttaattgata ccccacccct
     4921 caccgttttt tattactaaa aaatactaat aaatgactga agtggtgggg attgtatgag
     4981 cttataaact ttcattgtgt agtaaactaa acctcttatc tattaaggtt tacataatat
     5041 ttatattagc aactatgtat agtgaagcgg tcatttcgat ttttaaagtt tgagcataaa
     5101 aaagaagcag acattttaat tatgcctact cctcattgag tgaaaattta atccctactg
     5161 gtctaccttg tgtagtacct ttactatcgt attcaaagta atcttttata atatgatcat
     5221 catttacaaa tttatccata atcaaattga ttgttttttt gtgtcttgtt ttttgagttg
     5281 gtgttatgtt tttcatatcg cattcttcaa ataatttgtt gtaattaatt ttttgcatat
     5341 tatcaatata tttaatttta cctttgctat ttaatgcgcg tttgtattga ttaatacgtc
     5401 tgtataattc ggtgactagt attctatagt tattgttatt gactacgcct ttaggtgctt
     5461 gcaatactgt tttaggtacg tgttcaatat actttttgtg ttttgaatag tcatacataa
     5521 taggtttact tttgaataca atccagtctt tcgatgacgg ctcaccctca ctagttaatt
     5581 caataaattc tataataggt ctgcgaatac tattcataat gtgattggtc tctgcgtcta
     5641 taattccgct tccctcttct gcaaattgaa tttttactct aattgctgat agtttatcaa
     5701 gtctttgcat aaagtcttta tattctttgt ttcttatttt ttgttttgct ggataaccat
     5761 aagcctcacg aatgagtgaa gatattctac aacgaacaat atttttatct tcaatttctt
     5821 tttcatataa gttacttaca ccacgtaaaa ctatctcatc ttgtggatca attcgtattt
     5881 catcaatgtt tggatcggtt attataaact cgccactgtc tagcttcctt gtgttaggtg
     5941 ggtctggtga aaaaaaggat aactgttcat gtgttgcagt agttcttttt aacgcgtgcg
     6001 ataatggcga attatctact acatgaaagg tagcaagcgc ttgatcttgg tattccatta
     6061 attcaatact tttaaggtct tctggtgtag cattctttat ggaggtagca ttaggacgat
     6121 aattaaattt attgtttttg tttgtcattc gcttcaccta ccacaaaata agctttatct
     6181 aataccttgc tacttaaaaa ttcaccgctg tatccgtcaa tattcttagc aaatttttgt
     6241 ctagtcttta gttgttttag ttttctacta tacaatttat tagctatgtt taaatctttt
     6301 gtttcgaaat atccaatatt aacgttgtaa tatgttttca tcatatcgtt agaagcattc
     6361 catttactaa atatatcttt tatatcaatt ttattcttat caataaaggc ttgcatttca
     6421 tacatcgggt acattttagc ggtagctttc aaagtaatta aatcaagctc actttctaaa
     6481 tatttagctt ctgcactttg taatttatca ataaagaaac tctttacata tgattgtcta
     6541 aaggtttttt catttaataa ataatcataa acttcaatca tacaatcata tatatatacc
     6601 tttaattctt tttgtctaat gagccaatca ctcgcacgtt ttgttatagc gtcaaatatt
     6661 ttttcattca ttttataaag ttctcgtata ttataaactt cctctttaac ttcggattgt
     6721 tcgcccgctt ctacattact cgtcattaac tcacctatac aattaacaat acttttttcg
     6781 tttaattctt gatataaagg tgtttcaaag aaactagaaa actcataaat aaaatgagta
     6841 tctataaaag ccccgatata agataaaaaa tcagcgttct ttttatcttc aatacctttt
     6901 ataatcgcac ctactgaatt acttacagta gttagcgttc cgttttcata aggtattaat
     6961 aaatcatttt gtaaatcgta ctcacgcatt attacgcctc cctgtgaccg tttaaaacat
     7021 agataacata tcagtaacta tcgtataaca cataggtagc atgtaactac cctttttaac
     7081 ttctgctaac taatatttac ggaagttaat attttttata cagtgatgaa atatcatcta
     7141 ttttacttac aaactcaacg ttaaaaacta ggccaacttg tactgcatta taaaaatcac
     7201 gttcagccat ttttatttca tcgttattca aatataaaga atggccagtg tggttaagaa
     7261 attctgtaaa tttataagtt cctactggta tgttttggct gttatgaatt gcgtgtttta
     7321 ctgcttgttc tacgtttatg ttcatataat ccctctctct ttagtatttc aatgctcaga
     7381 aatgcgtctc ttagcgtcct gttgcagatt tgcgatagtt tattttattt aatagtttct
     7441 tagtttgttt ataagcgata taactactta ctgacgtaac aataacgcct gcaataactg
     7501 gaataacaaa gtctaacaat ttcataatat aagctcctta tttaatagca tgatagcgac
     7561 ccgtcacatt ggataggtcg ctttattata tttcaattaa aatatattta tgatactata
     7621 ctaaaataga gtgataattt attttattgc tcgtcattgt gttggccgtt gcctatttcg
     7681 tcgtgggcgc ggtcgttttc tttatctatc tttttaaatt tatctagtaa gttatcaaat
     7741 tctcttaaat acatacacat taaatcaatc gtttgtgtat tttttattct gtgttcatga
     7801 tatctataac cgtgtgctaa aatctcattt ttgtttaaca catgattagg ctcatgagta
     7861 aaatattctt cctcaaacca tgtaaaagat gtcattacat catcaatttt ttccttaact
     7921 atttccatgt cactcattat atttttaatt tcccaattca ttcgaaatta cctccattta
     7981 ttttttcaac attttcgatt tttcaaaatt taatacatta gaagttttaa ctccagctat
     8041 ctcatctaat tgttttgaag ttttttgaga tttttttata tcgtctttca atatgtttat
     8101 taaacgtgtt tctaatcttg taagtgatgc actttctcgc attactccgt caaattcttc
     8161 acgtacttca tcaatatagt taagcgtatt tgcgtttgcc tgaataagtc cgactaactc
     8221 ccaatgctct tgcattagtt tccatgtttc tttattatcc attgactacc tcctactatt
     8281 taccatttac aaaatcgcgg tagttatctt cagaaataat atatttattc ccgactttaa
     8341 cggctttcag tttccctttg cggatatatt ccctcactgt acgaggtgaa atatctaatt
     8401 gtgtagccac ctcatctact gtgtacgttt taattgccat aatataaaac tccttttatt
     8461 tttaattccc acatatttgg gaatattagt ttttaaaata atgcgtctgc aatacgtttc
     8521 tcactttggt ttttagatcg ttctaaatga tgtaagtaaa tcattgttgt attgatatta
     8581 gaatgacgta atagctgttg tgtttcttct aaactcccac cgttcataag gtttaatgtt
     8641 cctgccgtat gacgtaatga gtgagctgtt aaacggtcac tcgtataacc cgcttctttt
     8701 aatctttttt taactattcc gctaatgcta cgggttgtaa gtcgttgacc tttattatta
     8761 ttgcttgtag atgtaaataa cacttcgtgt tgtttgtcta acatttcacg atagcgtcca
     8821 atagctttga atacttgaag cggtaattta acatactccg ttttttcttc tctgccttta
     8881 ccttgtatat atagcgctgg actatcccca acatttctta tatcttccat atcggcacgt
     8941 gatacctcta ttgttcttaa tccacccgta accataagtg atattatggc gtaatctctt
     9001 gcacccgtca cagtagatat atcaatgtta tttaatacat ctttgatttg ttcagtcgtt
     9061 aagtaatctt tcttatgtgc ttggtctaat ttagccccct taatcttatc ggcgatatta
     9121 gggtaaaagc cttcttgatt agtccactgg aagaacaaac gaacagaaac aatataactt
     9181 ttaatggttg taggtttacg tcctttatca cgcaaataat ctctaaacgc taacacatca
     9241 ctacgtgtag gctgtgtaat attatgttca taaagatact taaagaattg ttttaaagct
     9301 cttgtatatg tttgtactgt cttttctgac gcgtcaatat aattcaagaa gcggtgcaat
     9361 aattctgtat ttacgctttg cgtatgttgg atctcatttt gattatgcat tgtttgtatt
     9421 tcattcatag ctttcaccct ttccgtaatt ctattataac atagtaacgt aaatagtcaa
     9481 cacatagaac gtatatagat aacatatagt tagttattat ttgcaaatag ataacatata
     9541 gtagtatgtt aaaagggcaa tagttggccg attaattggg cgatactgtt gcccgcctat
     9601 cgcaatagta gggcgttagg tgccaacata tcggcgacag tatataggct gttgggtctg
     9661 ttgcctatac attaaatcat tgatatgaaa ggagtgaaag gcgattgact aactctgttg
     9721 ggttcattac aactaaaaat gctagtaagc ttttaggtgt atccgcttca tcattaaggg
     9781 tttatgctca acatatggaa gctgtggggt actcatttaa gaaaatagaa aatgctaggc
     9841 aatttagtaa gcatgattta caaatcatta gcgaggcgtt agaacgttat aaactaactg
     9901 gcggtgctat aaaagacgca ttacattatg tgatagttaa agaagaaaaa ggggaagaag
     9961 aagcggaggc actgcaatct ctaccgaaag aacaacggtc acaagatgta gatcgtttag
    10021 acgttgagca atttaaacaa gaattatcaa aatatattag taataacatt aatagtacat
    10081 tagaaaaaca ctttcagaac gtcacagaag ctattacaga cgtttctaat acacaagacg
    10141 atactaaacc aatgcaagaa gaaattgagc gtctacgtgc tttaaatgag caattacaag
    10201 atgaacggga tcgatacaaa tcagaaaact taaatatgag taatgagata gaacaattaa
    10261 aatctatgag tatatgggaa tttaggaagt ggaagaaaga caaataacac tttttttata
    10321 atagttgtct gtgtcactta gtgttataat atagatacac actgatacgc cttgtatacc
    10381 gtttttaagc cttttgtcta tgctgtccta ccgtttggga tagcattttt ttatgcacaa
    10441 aaaaggagct tcgtatacca caccgatacg tcgctccttg tactttaagc tctttcttta
    10501 ttctctttag cattgcgtaa aacttcaatt cttctaaaaa taccaccgtt aagactttca
    10561 agcgtataaa tagctttatc tatatcctca tcactttcac ataagtaata accattgtgt
    10621 cgggtgcttc ctataatgta cccgt
//
