LOCUS       CP052966                8470 bp    DNA     circular BCT 09-FEB-2021
DEFINITION  Staphylococcus epidermidis strain HD40-5 plasmid pHD40-5_3, complete
            sequence.
ACCESSION   CP052966
VERSION     CP052966.1
DBLINK      BioProject: PRJNA546129
            BioSample: SAMN14732900
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buttner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Distinct clonal lineages and within-host diversification shape
            invasive Staphylococcus epidermidis populations
  JOURNAL   PLoS Pathog 17 (2), e1009304 (2021)
   PUBMED   33544760
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buettner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 402x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2020 13:46:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,434
            CDSs (total)                      :: 2,350
            Genes (coding)                    :: 2,289
            CDSs (with protein)               :: 2,289
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 38 of 61
            Pseudo Genes (incomplete)         :: 24 of 61
            Pseudo Genes (internal stop)      :: 24 of 61
            Pseudo Genes (multiple problems)  :: 21 of 61
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="HD40-5"
                     /isolation_source="nose; prosthetic joint infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pHD40-5_3"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2018"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(351..1274)
                     /locus_tag="HJI04_12135"
     CDS             complement(351..1274)
                     /locus_tag="HJI04_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020365845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="QRJ17136.1"
                     /translation="MINNLLSMENLNLNDVNSLFEKADYYEKALKDKKVIKDLEGKILA
                     TLFYQPSTRTRMSFTSAMYRLGGQVIDMGGPKVARTDDYFDESMSDTIKYIEQYSDIMV
                     MRHFQNYTVEKASKITHIPMINAGDGTNEHPTQALLDLYTMTKELNGLEDKVIGIGGDI
                     NCRVIRSIVIGLEKFDIKKIIFLLPNGEELNSDILNLLKNTDYSIVHNVERVLEQADIL
                     DIIPFELPDFNSAYSEKVDEKPSLENNLIVSKEKFNDKNRIPILSPGPREAELSSDTDD
                     MDNVIFTKQAYNGLLIRMSLLYYFLH"
                     /name="aspartate carbamoyltransferase"
     gene            complement(1267..2157)
                     /locus_tag="HJI04_12140"
     CDS             complement(1267..2157)
                     /locus_tag="HJI04_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein MoeB"
                     /protein_id="QRJ17137.1"
                     /translation="MNSKVIKYTADFNEKRYWERIKRNLGWLGDTDEEAKKRQKKISEV
                     VIGIAGCGGIGGAVAERLVRLGVRHIKVADPDHFELSNINRQFGASLDNLGRNKAEVVG
                     ESIFNISKDVNVDVYPEGINNSTADDFVEGCDYVLDKIELFELEARYALHKAFKKSERC
                     KFMLTVPVFGHRAFFFKWTKDSMSAEDYFGIKPGSELDEQNTRKIVYSLFPEYPQFPSK
                     ERLDDWLIYNKECPIFAGCPPLAQGLLVERLSFEIADLKDIPEYKELPSTPSYSMFDSA
                     SWETKIVEREENIID"
                     /name="molybdopterin biosynthesis protein MoeB"
     gene            complement(2276..2974)
                     /locus_tag="HJI04_12145"
     CDS             complement(2276..2974)
                     /locus_tag="HJI04_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="QRJ17138.1"
                     /translation="MYEKFADNILLIGHKTRLTMLIELSSGVSIPAGELAKLANVTPQT
                     ASEHLSKLVKANLISVNKKGRHCYYKIDNSEIVDAINALSIISPPMSSKSLKETTKKEK
                     LSYMRTCYGHIAGKIGVKFTEALLENDYLRESKDCYKLTQKGKFWLKKIGIATEKTMYS
                     KPIPKHIDWTERKNHIAGPIALQITEKLIELSWVKKSKINRCLNLTLKGKKAFKTYLNM
                     NITELENEIF"
                     /name="winged helix-turn-helix transcriptional regulator"
     gene            3086..4465
                     /locus_tag="HJI04_12150"
     CDS             3086..4465
                     /locus_tag="HJI04_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="QRJ17139.1"
                     /translation="MNSSKYFDHLVVFTAGIGMFLSTLDSGIINVALPTLSKSFNVDAS
                     FISWSITLYTLLLTGTIIIFGRLSDKYNRLNIYSIGLTVFLISSILCGFSNNVVELITF
                     RGLQGIGAAMLQGTATAIITTTIPEHRQGPALGTLSILLGIGPVLGPSIGGLLISIGNW
                     RWIFWINIPFIFIGLIGCLLLKKYIKEPKSVSIHLDIRGNSLLFVSIFCLLVSLTSWSH
                     HSFFNISVYSNFLIFIITFCLFIVWELKTNHPIINLRLFKNISFSSSIFAIFVFGGTTS
                     LGFIIPPYILEKINHLSSWQIGLVNLTSPLGLVLISKITGKLISRVGNTILMTVGLVIM
                     IASYTSLGLLQYILNPITISLLLLIYGIGGGFFLPSNTSAIMGTVSKDMQGTVGATQRM
                     VQNIGIAVYTAITSLFISNQSQTNQLITGASHAWLFASATLFLSLLPFLLKLLHHKSEA
                     T"
                     /name="MFS transporter"
     gene            complement(4500..4919)
                     /locus_tag="HJI04_12155"
     CDS             complement(4500..4919)
                     /locus_tag="HJI04_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002511307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ17140.1"
                     /translation="MPKKQNSKEKILTIRVSQSQYEKICELADIREMNVTEYIRHTAIG
                     NRIKPTIIEQIKESSEVSELFKKDNEALKSNNQALQHHIHQLEDEIDQLRQENDVFHHL
                     LQHFDSTAFLNFNTYRDDRPLKNAIKRLKEQYKSK"
                     /name="hypothetical protein"
     gene            5374..6582
                     /locus_tag="HJI04_12160"
     CDS             5374..6582
                     /locus_tag="HJI04_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="QRJ17141.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLKRTNLNFDLV
                     EDERHLYHRVKDRIEYYKKQGSRVQKNSVVMYSNIITLSKEEADRMGETRTKHYFKTCK
                     DYFSERFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     HLTRDIQNLKRKNHFLQEDSQQAQEDIDLYKQLRTISRDDFKKEEEKYQKEIKAYEASL
                     SGLRMNYRDLDKRNQHLTTQNNQLEHHLDVLNARHQAMNDMVRNDTRILFAGLRCALKD
                     MNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGGVSGIDHHIRKASVPIKKEAENDL
                     ELE"
                     /name="recombinase"
     gene            7218..8084
                     /locus_tag="HJI04_12165"
     CDS             7218..8084
                     /locus_tag="HJI04_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002452565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="QRJ17142.1"
                     /translation="MSNETVIYHNEMNLVPLRKFTSVEIDLFFAMCNKLKEQDTNKLYL
                     SFEELKYLSNYNYETRNLNRFIGDLQNIYKKMLEITYRREDEDVIEFFVLFNHFKIHKT
                     EKYLEISTNPDLKFVLNDITRNFTKFELKELTNLKSSYSKTMFRLLKQYKHTGYLKLSM
                     EDFRTRLDVSKSYQMCDITKRVLKPISKELTPIFNNLNINKVKAKKGRKIEYLEFIFDA
                     EKRIHNKRQPQITNIGKSRQYTNREKTPKWLDDKIYKQSQESHGEDAKLKQDREAFQRQ
                     LEEKWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            8190..8333
                     /locus_tag="HJI04_12170"
     CDS             8190..8333
                     /locus_tag="HJI04_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ17143.1"
                     /translation="MSNKYNIHPALYIGGFAVFFSFIFGFPVGTLMGLAIGIGISKKNK
                     NL"
                     /name="hypothetical protein"
     ARG             3087..4465
                     /name="mdeA"
                     /gene="mdeA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; aminoglycoside
                     antibiotic; penam; tetracycline antibiotic; disinfecting
                     agents and antiseptics"
                     /identity="40.29"
                     /coverage="101.32"
     Typing          5303..6579
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.948"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaccaacaa taattggaaa aatgagctta tctaatacaa atagcaagat tatcattgta
       61 tgtgacacct cctttgtgca cggaagtgct tttccagtat aacaaaaaac tacaatttaa
      121 aacggtgtgt tatcacgtga aattgtgaat aaagatacaa aatatctata aatgaaaaag
      181 tatgaaaaca cgtgagaaca acgatgtata aaagatgaat aaacgataat cacaagacaa
      241 aggcttaaac gctgttaaat caacgtttaa gcctttattg ttagttgaca taattactga
      301 ttatgggtag tgcttttgta tagcgtattc attttataaa tagctattaa tcagtgtaaa
      361 aaataatata aaagagacat tcttattaat aaaccattat atgcttgttt agtaaatatc
      421 acattatcca tgtcgtctgt atctgaagaa agttcagctt ctcttggtcc aggacttaga
      481 atagggatcc tattcttatc attaaacttt tctttagata ctatcaaatt attttctaat
      541 gatggttttt catcaacttt ttctgaataa gcagaattaa aatctggaag ttcaaaaggt
      601 atgatatcta atatatctgc ttgctctaat actctttcta cattatggac tatagaataa
      661 tcagtgtttt ttaataaatt taaaatatcg ctatttaatt cttctccatt tggcaataga
      721 aatataattt tcttaatatc aaatttttct agacctataa caatagaacg aatcactcta
      781 caattaatat ctccacctat tcctataact ttatcttcta aaccatttaa ttcttttgtc
      841 atcgtatata aatctaataa agcttgcgta gggtgctcat ttgttccatc tccggcatta
      901 atcataggaa tatgggtgat cttcgatgct ttttcaacag tataattttg aaaatgacgc
      961 ataaccatga tgtctgaata ctgttcaata tatttaatag tatctgacat actctcgtca
     1021 aaataatcat cagtgcgtgc tactttaggt ccacccatat ctatgacttg tccacctaat
     1081 cgatacatgg cacttgtaaa actcattcta gttctggtac ttggttgata aaaaagcgta
     1141 gcaagaattt taccttctaa atccttaatt acttttttat cttttaaagc tttttcataa
     1201 taatctgctt tttcaaatag gctattaaca tcattgagat tcaaattttc catcgacaat
     1261 aaattattaa tcaattatat tttcctctct ttctacaatt tttgtttccc aagaggctga
     1321 atcaaacata gaataacttg gagtcgaagg taattcttta tattctggaa tatctttcaa
     1381 atcagcaatt tcaaagctta atctttctac taaaagacct tgagctaaag ggggacatcc
     1441 agcaaaaatt ggacattctt tattataaat taaccaatca tctaatcttt ctttggatgg
     1501 aaactgaggg tattctggga aaagagagta aactattttt cttgtattct gttcgtccag
     1561 ttctgaacct ggctttatac caaaatagtc ttcggctgac attgagtctt tagtccattt
     1621 aaaaaagaaa gctctatgac caaatactgg tacagttaac ataaatttac atctttcact
     1681 ctttttaaaa gctttatgta gtgcataacg agcttctaat tcaaacaatt caattttatc
     1741 taatacataa tcacaaccct ctacaaaatc atccgcagta ctattgttaa ttccttcagg
     1801 ataaacatca acatttacat cttttgaaat attaaatata gattctccaa ctacttccgc
     1861 tttatttctt cctagattat ctaaagaggc gccaaactgt ctattaatat tagagagttc
     1921 aaaatgatct ggatctgcta cttttatatg acggacaccc aatcgtacta gtctttcagc
     1981 tacagcaccc cctatacctc cacatccggc aatacctatt acaacttcac taattttctt
     2041 ttgacgcttt ttcgcttctt catcagtgtc tcctaaccaa cctaaatttc gtttaattct
     2101 ttcccaatat cgcttttcgt taaaatcagc agtatattta ataactttag aattcattat
     2161 aaacctccaa aaaatagtgt tttataataa atacataact cgatacagaa aaattaaaat
     2221 ataagtggtt taatacactt acaataacta cataatcata aaactctttt aattgtcaaa
     2281 atatttcgtt ttctaattct gtaatgttca tatttaagta tgtcttaaaa gctttttttc
     2341 cttttaaagt caaattaaga catctattga ttttactttt tttaacccaa gataattcaa
     2401 tcaacttttc agttatttgc aatgcaatag gacctgctat atgatttttc ctttctgtcc
     2461 aatctatatg tttgggaata ggttttgaat acatagtttt ttctgttgca atacctattt
     2521 tttttaacca aaactttcct ttttgagtca acttataaca atctttactt tctctaagat
     2581 aatcattctc tagcaaagct tcagtgaatt taacacctat tttcccagcg atatgaccat
     2641 aacaagtacg catgtatgat aatttttctt ttttcgttgt ttcttttaac gatttgctac
     2701 tcataggtgg agatataata gataaagcat taattgcatc aacaatttca ctattatcaa
     2761 ttttatagta gcaatgtcgt cccttcttat ttactgaaat taaattggcc tttactaatt
     2821 ttgaaagatg ttcactagct gtttgaggag ttacattagc taatttagct aattctccag
     2881 ctgggataga taccccactt gataattcga ttaacatagt taaacgtgtc ttatgtccaa
     2941 taagtaaaat attatcagca aatttttcat acatataatg cctcctttct attagtacaa
     3001 tttaatcata cccatataat atttcggtgt taaacgaaat attatatggg tatgattaaa
     3061 ttagttgaat gaggaggtat gatagatgaa ttcgtctaag tatttcgatc atttagttgt
     3121 ttttacagca ggaataggta tgttcttatc aactctagat agtggtatta tcaatgtggc
     3181 gttacccact ttatctaaat cttttaatgt tgatgcttct tttatttctt ggagtattac
     3241 gttatatact ttgttactta cagggaccat tattatattt ggaagactga gtgataagta
     3301 caatcgtcta aatatctata gtattggact aacagtgttt ttgatatctt ctattttatg
     3361 tggattttca aataacgtgg tagaattaat tacttttcgt ggtttacaag gcataggtgc
     3421 agctatgtta caaggcactg ctacagctat tattactaca accattcctg agcatagaca
     3481 gggaccagca ttaggaactt tgagcatttt attaggaata ggtcccgtgt taggtcctag
     3541 tattggaggc cttttaatat ctataggaaa ttggagatgg atattttgga ttaatattcc
     3601 attcatattt ataggcttaa taggctgttt acttttaaaa aaatatatta aagagccaaa
     3661 aagtgtatct attcatttag atatacgtgg aaattcgcta ctatttgtat ctatattttg
     3721 cttattagtg agtttaacat cttggtctca ccattcattt tttaatatat cagtatatag
     3781 caatttctta atatttatta taactttttg cttatttatt gtgtgggaac taaaaactaa
     3841 tcatcctatc atcaatttac gtttattcaa aaacatatca ttttcatctt ctatatttgc
     3901 aatatttgtt tttggaggta caacgtcttt aggctttata attcctccat atattttaga
     3961 aaaaatcaat catctttcat cttggcaaat aggactagtc aatttaacat ctcctttagg
     4021 attagtactt atatcaaaaa tcacagggaa attaatatct cgtgttggta acactatttt
     4081 aatgactgtt ggactagtta ttatgattgc ttcatatact agcttagggt tattacagta
     4141 catattaaat ccaataacaa taagtctatt gttattgata tatggtatag gtggaggatt
     4201 tttcttacca tctaatactt cagcaatcat gggaactgta tctaaagata tgcaaggtac
     4261 tgttggagca actcaacgga tggttcaaaa tataggtatt gcagtttata cagcaattac
     4321 ttcactattt ataagtaatc aatcacagac aaatcaactg ataactggtg ccagccacgc
     4381 atggttattc gcatcagcaa cacttttctt atcactatta ccttttttat taaaattatt
     4441 acaccataag tcagaagcta cttaatataa taaaagcaag tgatttataa aattataaat
     4501 cacttgcttt tatactgttc ttttaatcgt ttaatcgcat tttttagtgg acgatcatca
     4561 cgatatgtat tgaagtttaa aaatgctgta ctatcaaaat gttgtaataa gtgatgaaag
     4621 acgtcatttt cttgtctcag ttggtcgatt tcatcttcca actgatgaat atgatgctgg
     4681 agtgcctgat tgttcgattt gagtgcctcg ttgtcttttt taaacaattc actgacttca
     4741 ctggattctt taatttgttc gatgattgtc ggtttaatac gattaccaat ggctgtatga
     4801 cgtatgtatt cggtcacatt catctctcga atatccgcaa gttcacaaat cttttcgtat
     4861 tgcgattgac ttacacgaat tgttaaaatt ttttcttttg aattttgttt ttttggcatt
     4921 gaaccacctc atgattaata tatgataagc atacatatgt gtgcttattt ttatattaat
     4981 ataacataca tatgtatgct aataaatatg tatgattcga ggtggtatga gctaacattt
     5041 ggcatacata ttttcaaaca tagttgtttg ataaggtgta tgtcatttgt tagctataga
     5101 acggcattca aaaatatatt tttgaatgcc gtaatggcct cgccagagcg aagataactc
     5161 ttacatttga atgtaagagt atatcgagct atactctcat tgcggaatca gcctcacatg
     5221 tttgaggcgt agcggtgaga gtatccatca ttttacataa gcgaagcatt gtcgaaggta
     5281 gacaatcaca gcgcttgtaa aatgatattt gcgaaggttc tcgaaagtag agaaccacag
     5341 acaaatcaaa aagcttcggg gagggcgctc aatttgaaat tagcttggaa tacgcaaaaa
     5401 aataaaatga gcgatgttaa aggcaaagag atggaacaag aacgtaaagg gagaatcaaa
     5461 aacgatgaga ttgatttgaa acgaacaaac ttaaactttg atttagttga agatgaacgt
     5521 catttgtacc atcgtgtgaa agatagaatt gagtactata aaaaacaagg ctcacgtgta
     5581 cagaaaaata gtgtggtgat gtattccaat atcattacgt tatcgaaaga agaggccgac
     5641 cgtatgggag agacgcgtac aaaacactac tttaagacat gtaaagatta cttcagtgaa
     5701 cgttttggtg aagccaatgt cgtttcagcg aaagtgcata tggatgaatc agcgcctcat
     5761 atgcaccttc actttatacc tgttaatcat caagggcggt tatcagcacg tacagctatg
     5821 aatcgacaag ccattaatca catccatgat gaattaacaa cacatctgtg ccagcaaggt
     5881 tttgatgttg aacgtgggag tactgatgat aataaaacgt atattcaaga tattcatgaa
     5941 tataaaaaga aagcgaaagt tttaagtgaa ttagaccaac aaattgaaac gaaaaaacaa
     6001 ttgttgaggg atatcaacga tttacacgat gaaaaagaac atttaactcg ggatattcaa
     6061 aacttaaaac ggaaaaatca ttttttacaa gaggattctc aacaggctca agaagatatc
     6121 gacctataca agcaactgag aacgatttct agggacgatt ttaaaaaaga ggaggaaaag
     6181 taccaaaaag aaatcaaagc gtatgaggcg tctctgagtg gccttagaat gaattaccga
     6241 gatttggaca agcgcaatca acatttaaca acacaaaata atcaactcga acaccattta
     6301 gacgtgttaa atgcacgtca tcaagcgatg aacgacatgg taagaaatga tacacgtatt
     6361 ttgtttgcgg gacttagatg tgcattgaaa gatatgaaca ttgaattaag cgcagaaatt
     6421 aaatatcaac gtatgccaga agtcggtatt gatcccgata cggtggatca gggggcttta
     6481 acacatgcat atcaaggtgg tgtgtcaggg atagaccatc atataagaaa ggcatcagtg
     6541 cctattaaaa aagaggcaga gaatgactta gaattagaat aaatcaataa aaataagcac
     6601 tcaaacaagt atgatactcg cctgagtgct tatctcatga aaagaggaga taaaaattta
     6661 ataagtatga ttaaccgatt aagaacaatc attaccttac ttacagcgct tattaaatag
     6721 ttctgtaagg aggaacaata cctttgcatt tctatattat tataagaatt ctaaggtagc
     6781 aaagatattg tattggaaat gatagatgtg agtatgattt ctagattaat tgatgaagat
     6841 attttttaaa gtttattttt tttgtgttcg tttctttaga agtatatttg aatgaatttg
     6901 gagtatttca ctccgacctt tttaatatat ttttataaat ctttttataa acctttttag
     6961 gtgtgcttta atccttagag cgacagtaga ttccacaact taaaaaggtc attttgccac
     7021 ataaaaaagg tcattttgcc acataaaaaa ggtcattttg ccacataaaa aaggtcattt
     7081 tgccacataa aaaaggttaa attaaaataa tgaccttttt tatgtggcaa atacttttaa
     7141 taaaactatt atagtgataa tatgtatatg atatacaagg acttataaaa ataacgacct
     7201 tttttggagt gatatatatg tcgaacgaaa ctgttattta tcataatgaa atgaatttgg
     7261 ttcctttgag aaaatttact tctgttgaaa tagacttatt ttttgctatg tgtaataagc
     7321 taaaagaaca agatactaat aaattatatt taagttttga agaacttaaa tatttaagta
     7381 attataacta tgaaactaga aatcttaatc gttttattgg agatttacaa aatatttata
     7441 aaaagatgct cgagataact tatcgaagag aagatgaaga tgtaatagag ttttttgtac
     7501 ttttcaatca ctttaaaatt cataaaacgg agaagtatct tgaaatttcc actaatcctg
     7561 acttaaagtt tgttttaaat gatattactc gtaattttac aaaattcgaa ttaaaagaac
     7621 tgactaattt aaaatcttcg tactctaaaa ccatgtttcg cttacttaaa caatataaac
     7681 atactggata tttaaaatta tcaatggaag attttagaac tcgattagac gtatcaaagt
     7741 cttatcaaat gtgtgacatt actaaacgtg tattaaagcc aatatctaaa gagcttacac
     7801 ctatttttaa taatcttaat attaataaag ttaaagctaa aaaaggtcga aaaattgaat
     7861 acttagaatt tatctttgac gctgagaaac gtattcacaa caaacgacag ccacaaataa
     7921 ctaatatagg taagtcgcgc caatatacta atcgtgaaaa gacacctaaa tggttagacg
     7981 acaagatata taaacaatct caagagtcac atggcgaaga tgcaaaatta aaacaagatc
     8041 gagaggcatt tcaacgtcaa ttagaagaaa aatgggagga ataataattt aaatgagcta
     8101 catgtatatt ttaatcttat ttccattaat aatgggattt atcgtatcaa taaaaaagaa
     8161 aaaacatgac taaggagata taagaaggta tgagtaataa atataatata catccagcac
     8221 tatatatagg cggatttgcg gtatttttta gttttatatt tggatttcct gtaggaacat
     8281 taatgggact agctatagga ataggaatta gtaaaaaaaa taaaaatttg taaaagagtt
     8341 tacagatgaa gaagttacac gatatactgt aattaacaaa tagcagatat atcattgtat
     8401 gtgaaaaccc ccagcgtgcc ggctgggggt ttctttttac ttatgttctt gttgtaatag
     8461 ataaagaaca
//
