LOCUS       CP052960               13570 bp    DNA     circular BCT 09-FEB-2021
DEFINITION  Staphylococcus epidermidis strain HD46-1 plasmid pHD46-1_1, complete
            sequence.
ACCESSION   CP052960
VERSION     CP052960.1
DBLINK      BioProject: PRJNA546129
            BioSample: SAMN14732902
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buttner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Distinct clonal lineages and within-host diversification shape
            invasive Staphylococcus epidermidis populations
  JOURNAL   PLoS Pathog 17 (2), e1009304 (2021)
   PUBMED   33544760
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Both,A., Huang,J., Qi,M., Lausmann,C., Weisselberg,S., Buettner,H.,
            Lezius,S., Failla,A.V., Christner,M., Stegger,M., Gehrke,T.,
            Baig,S., Citak,M., Alawi,M., Aepfelbacher,M. and Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2020 13:42:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,672
            CDSs (total)                      :: 2,591
            Genes (coding)                    :: 2,507
            CDSs (with protein)               :: 2,507
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 43 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 22 of 84
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="HD46-1"
                     /isolation_source="nose; prosthetic joint infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pHD46-1_1"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2018"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            1..936
                     /locus_tag="HJI06_13275"
     CDS             1..936
                     /locus_tag="HJI06_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="QRJ12650.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(1161..1400)
                     /locus_tag="HJI06_13280"
     CDS             complement(1161..1400)
                     /locus_tag="HJI06_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="QRJ12651.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1417..1686)
                     /locus_tag="HJI06_13285"
     CDS             complement(1417..1686)
                     /locus_tag="HJI06_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ12652.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            1895..3817
                     /locus_tag="HJI06_13290"
     CDS             1895..3817
                     /locus_tag="HJI06_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QRJ12653.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            4091..4504
                     /locus_tag="HJI06_13295"
     CDS             4091..4504
                     /locus_tag="HJI06_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="QRJ12654.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            4581..4814
                     /locus_tag="HJI06_13300"
     CDS             4581..4814
                     /locus_tag="HJI06_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ12655.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(4863..5567)
                     /locus_tag="HJI06_13305"
     CDS             complement(4863..5567)
                     /locus_tag="HJI06_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ12656.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5718..6416)
                     /locus_tag="HJI06_13310"
     CDS             complement(5718..6416)
                     /locus_tag="HJI06_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="QRJ12657.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            6580..6678
                     /locus_tag="HJI06_13315"
     CDS             6580..6678
                     /locus_tag="HJI06_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QRJ12658.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6855..7767)
                     /locus_tag="HJI06_13320"
                     /pseudo=""
     CDS             complement(6855..7767)
                     /locus_tag="HJI06_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(8186..8746)
                     /locus_tag="HJI06_13325"
     CDS             complement(8186..8746)
                     /locus_tag="HJI06_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QRJ12664.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(8997..9563)
                     /gene="qacR"
                     /locus_tag="HJI06_13330"
     ARG             complement(9001..9563)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9742..11286
                     /gene="qac"
                     /locus_tag="HJI06_13335"
     CDS             9742..11286
                     /gene="qac"
                     /locus_tag="HJI06_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="QRJ12660.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVSGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            11491..12051
                     /locus_tag="HJI06_13340"
     CDS             11491..12051
                     /locus_tag="HJI06_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QRJ12661.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            12127..12249
                     /locus_tag="HJI06_13345"
     CDS             12127..12249
                     /locus_tag="HJI06_13345"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="QRJ12662.1"
                     /translation="MAPYTNKGYAVKELCSYLQINMDDVYTIGDRKLIYLCLIP"
                     /name="HAD hydrolase family protein"
     gene            complement(12370..13131)
                     /locus_tag="HJI06_13350"
     CDS             complement(12370..13131)
                     /locus_tag="HJI06_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QRJ12663.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             9743..11283
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          2..936
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1896..3748
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atgcccaatt ttgaaaaata taatttatca caagtaaaaa ctgaaagatt ttatcaactg
       61 cctaaatatt tatttgaaga tgcatatttt aagaaaatgt ctgcagaagc caaaattatg
      121 tatgcgttat taaaagatcg ttttgaatta tccctccaaa atgaatgggt agataaaaat
      181 aataatattt actttatttt cagtaataaa catttgtgtg aatacttagg ttatgcagaa
      241 caaaaaatta taaaattaaa aaaagagtta ataaaattta atttactaac tcaagaacgt
      301 gttggcctta ataaaccaaa tagattatac ctattaaaac ctaattatga cattgaagcc
      361 agtcatatca aggaacttcc aaattcacag ttccagaaca atgaatttgg aagttctaga
      421 actgtgaatt taagtggtca agaacttcca aattcacagt ctaatgatac tgattataat
      481 gacactgatt atattaagac taattataat gatatgtatg atttgaatga taggaaatta
      541 acatattcta gtaatcatac aaatcattca aatcactact attcaaaatt caatgatgaa
      601 gctttaaaat ttcaattact tgaagaacta ccacaaagta ttcaaagtta tttaagtaac
      661 ttttctgtat ctgaaattaa acttattaaa tctgtgttat taaaagccaa aacatccttc
      721 aacaattcta ttgatgcata ttatttatta gaagatatgg aatttgaaat tgttaatgtt
      781 cttaagcgtt ttaaagctac attaattcaa aaaaatgaaa ccgttgaagc aatgcaaggg
      841 tacttaatga aatctcttaa atctgaattt gcagaagtac atacgcttaa taaacgacgt
      901 gatcatttac caatcacttc tttatttaat caataataca acttaataag ccaacaatga
      961 tccaattaga gaatatcttt aattagatgt tctcttaact gtaagcgaac tttattttca
     1021 atgaaatgta attaattaag taagtgggac taaaactttt cttttttagt cccattttta
     1081 taaacaatta ttattcattg ttactactat cagcaatttt ttctttttag tgaaacccaa
     1141 gtatttgtaa gcatgataaa ttacttcttt ttatatttat tgggtttatt tgtattaatg
     1201 tagttggaat acatctttaa caattcttct gtttcatcag gacttaaatg ctcacattca
     1261 aaatactttt caagcattgc accttttcga atcagtctat gtgctctttc ttttctatct
     1321 aaatttttta atctatgctt tcttaattct gctttttcaa tttcagcatt tatttttttg
     1381 agatgtttaa aatcattcat cgtatcactt actattctag ttaattttgt cttctgttaa
     1441 attttccttg ttctcgttgc tgctattttg ttcgatatct ttattaaatt ctccaatgac
     1501 tttatccata attttataca attcatcact agagttgatc atatttaaat caagtttatt
     1561 taaaaaataa ccaccaattt tattagatag ttgttcttct tgttttttct tttgctcttt
     1621 cagttgtgca atttgttttt ctaattgact taattttgta atatctgttt gtttaatttt
     1681 agacattaaa aaactccctt agtttatcta tataaagcat accataattc attgggtaag
     1741 tcgcacatcc atggtataat ataaccagtt cttaagacac tgcactgccc atttacatgc
     1801 aaattgaaaa ttggcataaa aatgggcaac catactggtt gatcattata gttcccacag
     1861 gggtaaaaaa acagaaaaga gaggaggtaa aatcatgaat aaaggttact ttatgttgag
     1921 agtgactaac attaatcgag caacaaaaaa tatcgttgcg tcagcgtctt atcgttctga
     1981 tgaagcatta tatagcgaac gtacagatga aaaaattaag tttagaaatc atactattaa
     2041 gcctgaaagc atgattttaa ctcctcaaca tgcacccgaa tggactaaag atagacagcg
     2101 tttatggaat gaagttgata aagttgaaaa gaataatgcg aaaacaaaaa atcctagatt
     2161 agcaaaagaa gtcttattaa gtttgcctaa tgattttgat agagaggtac aaactgaatt
     2221 aactaaagat tttataaaaa gtgagtttgt agacaaagga atggttgctg atatttcaat
     2281 tcatagagat gatataaaca acccacacgc ccatgttcta cttactcaaa gaccgtttaa
     2341 tgcagacggt acttggggga aaaagacaaa aacgagaacg caatatgatg aaaatggcaa
     2401 tgcgatttta aataaaaatg gtaataaagt tagaaaacaa gaacgatttg ccgactttga
     2461 tttcaaagaa gttcgtaagc attgggaatt aaaattaaat caatactcag aacgtgaaca
     2521 aagtttaaga cgatatgata gtcgttcttt tgaagcgcaa ggtttagaaa aaattgccga
     2581 aattccactt acaagagaag aatatagatt agaacaaaat gaaagaaaac gctgtgaaaa
     2641 agagggtatc gagtaccaac ccgttactta ttacggacag aaaaatgaac aaattagaag
     2701 atataatcgt ggcgaagtat cacaattatt tagtgataaa gagaaagaac aagcacaaaa
     2761 tgaaattaga ttatttattc aaaatgctaa cgaacaagta agtaaaaatg aacaaagtta
     2821 ttctatttta tttgatagat atcaacgtga tgttggctat tcagaggcga aagaaacaat
     2881 taaaaatacg tttgacagtg cgtctacctt tggtcgtaaa attcaaaatg atttacttaa
     2941 aaatgaaatt aagagacgtc aattaaaatt ctatgctaaa aattatgatg aaaattatca
     3001 gtttagaaaa gaaattgaaa gtaaaggata tagtcaacaa gagtttgtta atactgttga
     3061 aactaactat aaagcgttac tcaatgagaa gaataaatta gaaagtagag aggaaaaacg
     3121 taaagagatt tatcaatcag ctaaagatgt ttatctaact gaaagtgaaa aaaatcatct
     3181 cattgttcag caaatgtacc ctaaatctca tcatcatttt agccatgatg aaaaagcatt
     3241 tattgttgat gaggcacaaa aaggtaatat tataaaagca actcatgttc aatacctgtt
     3301 caaacaaaat aacaatattc ctaatgatgt attgctagaa gatacttata aaaaagcgag
     3361 taaagatatt ttctttagtc aaaaacatct taaaaattta gaaaaagata gctttgaata
     3421 taacgttgag ttgcaattta ttaaggaaaa agaaaaagaa attgatagtt ataaagaaca
     3481 tattgatgat gatttaagaa tagatatagg cgataaaaac tttgaaaaag tcaaagacca
     3541 aacacctaat gaaaaagttc aattactcat taaacttgag aaatataatc atgtcgatca
     3601 agaagaagtt atcgaaaaac atattaatca agtagataga aatgatcatg acaatcgtat
     3661 tgaacatgaa aatgaacaaa ctaatcacat taatttacca acgataaatg ctagtgactt
     3721 tcttatgtac tatgcgaacg aaactgaaaa gcaaaacaat attaatcatc agaaaaagaa
     3781 gaagaaacaa gagagaaata accaaatgag aagatagtaa gcagttgaaa tcatacaaag
     3841 ttcattcttt aataataaga aacttaaccc aatgtggtaa attagctttg gtttaaagaa
     3901 aacatttata tacttagata aggtacattc tatgtattat cacaatgtat tttaaaacgt
     3961 gtataattat agaaaaacct tgatattatg cgattgttca catatttcag cataaaaaat
     4021 actaatgttc ataaatatcg atagaaaaaa ttgagtttaa atatatctta aagttaagga
     4081 gaaaaataat ttggaaacaa aaattggaca tcaaatacaa aagttaagaa aaagtaaaaa
     4141 catgtctcaa gaaaaattag ctgaaaagtt aggggtatcg agacattcta tttctaattg
     4201 ggaaagagaa gtaagtaatc ccgacttaaa aacaatttta gaaatcacta aattatttaa
     4261 tgtttctcta aatcaattaa ttaaaggagt ggaaatcatg caagttaata aatatgttta
     4321 ctctgcatta gcattttttc taggaggttt cggggctcat aagttttatg ttaagaaaaa
     4381 taaaaatgct cttctttatt tactattttg ttggacggga ataccgggtg tgataggtat
     4441 ggtagaaggt gttttaacac ttatgcgtcc gagcgatagt gaaaacaata tagaaataaa
     4501 ttaaaccaaa aataaaaagc cgatgtatcg gatttttttt agtgtacatg tgaatattaa
     4561 aaatgatgga gtgatttcac atgaacacat tcgttttcgg cgtgattaca aagcgattag
     4621 aagaaatagg ttttaatgta cacaaggtgt ttaaaatgcc cgaaaaacag gcactatttg
     4681 aagatttgta ccattatgtg agatacgtta aaccatgcaa ggctgaacta acaatggtgg
     4741 cattagatag tgatgaattg attcattact ttatatgctg ttacatggaa cgttatgctt
     4801 atcttttgaa ctaatgataa aaaagacgcc taattttagg cgccttttta ataatctact
     4861 aactacttat tatatctaat tttacttgct aacttagcta ctgttttatt gtcgtagggt
     4921 ttaatataga ctgtttcaat attctcctct attgcttttt ttgttatctt attcatttga
     4981 ctataatcac tattttgtga attttcccat ttataaggga tattattata tacattaatt
     5041 gttccatcac cattactgct atatgttaca gacccaccag ctctcatagg agcttctaat
     5101 ttcacaactt tttctttata aattgcacta tttttggctt gtggatttac ttttgagcct
     5161 tttggtattt ctgtaacata aacattttta atacctttta aatcatttct tgtagatatt
     5221 aactgatacc aaactcgtgc atattcaatt tctttcgagt taaaattatt taagtaactt
     5281 ttatttttat cagtagattg ttgcttctta ttactatcat tatttttttc ttgtttttta
     5341 ttatcatttt tttctttatt ttgtttatta ctactttctg aattatccgc actatcagat
     5401 gtgttagttt tttcgtcaat ttcatttgat gcttcattat ctttgttttc tgaggtgtta
     5461 gtatcactag aattattatc attgtgatgt tctgaatcgt tattattaca actcactaaa
     5521 attaccgata aactaacaat taatataggt aaaatgattt ttttcatatt acaactcctt
     5581 ttcagtattt caaattagat tatgttaata ttttttcagc aagaaaacgt aaattacatg
     5641 agttgaaaaa tatgtattaa atatataata aaataaccac atgttttaca taaacacggt
     5701 ggttatttta tcaattttta tattgccttt tttctgaata ttaaaagact tatacctaat
     5761 attagtagtc cagaagcgcc aattattgac aatatatcac tttggttgct ttctcctgtt
     5821 tctggtaata cttgagagtt tgaatcttga tttttcattc cttcagaact cttgttttta
     5881 ttttcagtta caggtgtcaa cacaccttga ggtgtattaa tagttttatt ttcttcatta
     5941 gctatctttt gactttcatt ttttacaatt tgattatatt tttctgtgga tacactatta
     6001 gggttaggaa catgactttc taattcttgg tattcttctt gagtcataca attaggtatc
     6061 acccctccga aaggtctttt accttgttga gttctctcat tattttgtat atcagtactt
     6121 tgtgattgat attcattaaa tacacttgaa actttctcat tatactcgat ttgagttaat
     6181 tcccctgaat ctttctgttt agaaagttca cttatttgat tttgtgcaga ttgtgggagg
     6241 gatgaagtat tgaagtctac attagcccaa ccatctccat ttgtatctgc gctatttggt
     6301 aagttctctc cgtatacggt tttggcacca tcctcagcat tggctgtcgc tgaaaaacct
     6361 gtgaatgtta atgagctagc caataataaa gttggaagta atgatcgttt tttcataaaa
     6421 ttatctcctt taatagaatt tgtattttca ttatcacata gatacattaa acataaaatg
     6481 acttatcgga agtgcatatg cttaacaatt cacaaatgat ataatataaa agtcgtcgtt
     6541 ctacttctgt gctggtttag catggaagga ggtgacacca tgttcagcat catatttgta
     6601 tctatagtag ctcctattat agtaggggtt ataatcacgt tgttttcaca ttggttgaat
     6661 aatcgtgata aacaataaag cgacgacaca gtaataacac caaaaagagc ccgataacta
     6721 ctggaatagt tatcgggctc ggtgtttacc atgttcaaca catatttgtt atctaaatta
     6781 taacatcata gtgtggagga atgcaaaatt gaataattag tatgttaagt ctatactata
     6841 ctgattagta agtactaatt atcatctttg ttattgttac ccctttcatt ggcttttgaa
     6901 tctaattctt tttgaaattt tttgtgtgat tttgaagaag tgtttaaaaa gaaataggtc
     6961 atattatcca tacgatgtcc ttttataaat tcattgataa attgtttttt ttgtcatttt
     7021 tttggttgta ttacgttgtt tttgcaatct aatgatttct tcttcaatgt atactcttat
     7081 catcaaatct aaactagatg tctgattata agtagtgaat aattttggaa ctaataaagt
     7141 tttattacca tcaaaaagaa atggagtgaa atcataagtt tcccatatac cgttagtaga
     7201 gaaataataa tatgtcacag tttctttaga aagaaacgat ttcatttctg gaaatgactg
     7261 aatcatggat ttattaaaat caattataac attcatgatt atattacttg ttaaatctga
     7321 taatttatca tcaccaaaat ttctgattaa tggtgctttt tgaggtattg ttaatccgtc
     7381 atcatttaag ataatggaaa gaatatcttc attcataaat agattaatca attcagatgc
     7441 tgtagtacct tttcctgtat tacctttctg agaaaaacct aagcgtaaat ggtgtggttc
     7501 tgaaaaatga gagaataatt ttctaaattc attacgcttt tgggtatcag aaaaatcact
     7561 aagatgtata tcgataattt tttcaaaaaa aacatcaatt tgctcttgtg ctctttgagc
     7621 caaagtcgca acttttccaa cttctctatt agatattact ttaataaggt ttgggtctat
     7681 atattgaaga gtgtcttctt caatagaaat atttaaataa tcagttgatt tagcatcatg
     7741 attgaatttg gtacatacta gtgtcactct atatcatcct tctgtaagtt ttttgtttac
     7801 ttccaatata gaatgatgta ttattgtaat tgtcgattta caataagttg ttattctagg
     7861 tggaagttta ttgcttttga tgtatagata gacttaacct tttcattata gcatactata
     7921 gaagaaaatg caagcaaaag catataaaca acaaatgttg tttatatgct tttgcttttt
     7981 tgttaattgg agaggacaag gtgaatatta acagtcaaaa tccagttatg tcggttaatg
     8041 aatcgatgaa aattttcaaa gctcaagtaa tgcttagaaa tttaaagcta ttaagcgagt
     8101 ggattgttaa aggggactga agttaaagac actttttaag aagatttttc aattaaaggg
     8161 tatattaaaa ctttaattta gtactttata tttttcttga ctctttaata atcctatata
     8221 cagtagaacg actcactcct gtttgttcag caatttcttc accagacagc ttttgttcat
     8281 catataagaa tttgatctct cttttcttat gatctggtag tgaaggtctt ccaccttttc
     8341 tccctcttgc tcttgcagct tccaaacctt ttttagttcg ttcacttaat aaattagctt
     8401 caagttcagc gaatgcactc atcatggtaa agaacatctt gcccatcgca tctttcgttg
     8461 acacattcat atctataata tgtaaatcaa tgctattttc atccaaccat tgagatagct
     8521 caattaattg ttttgttgta cgtccaagac gatctaattt atagacaact agaatatctc
     8581 cttcacgtaa ataatctaaa cacttatcaa gttccgttct tttagtttta cgaccactcg
     8641 ctttctcgct aaagatacgt tcacaaccat attcctcaag tgtatcaatc tgtccatcaa
     8701 gactttgatc ttgtgttgac acacgtgcat aaccaatttt agccatttga acaccacctt
     8761 ttttaatcgt tttatgggta cttactaatg aaatatagtt ctcgatgtaa ataaacacgt
     8821 ctgtcagcat ctaaaaactc atgattttcg atatttatga tacattgatt ataacacaag
     8881 tttatagact taaatatcca agaaaaacct cttaatatta agttaagagg ttttgtgtca
     8941 aaaacgaacg taatttagaa ataatattta ttggtatttc aagaatatat atataattat
     9001 ttactaagtc catttaaaaa aatttgagag aacttgttca taagtttaat tctttcatta
     9061 atattttgtt catgtgtaaa ggtaacaatt ccattaacag cgtttgctgc tattttacta
     9121 acagcattaa catcattaat acaccattcg ccatttaaat taccctcctt aaaaattaca
     9181 tgataagcat ctatatattt gttttctaat ttattcattt tttcattaat gctattagtt
     9241 ttataatatt cagtgtaaaa ttcaattatt gcgttttgaa gtggataata gtattcggtt
     9301 gttaaagata gttcattata taaataaaat ttttctctat tagttttaca tttgatttgt
     9361 tcctttttcc actgttcttg ccatttagat tcttctatat ttaaaatttc taaaaataga
     9421 ttttcttttg ttttaaagtg ataataaaga ttccctttac tactttctga taatttaaca
     9481 atttctccag tagtagtggc attatatcca ttttttataa ataattcctt tgcgacacct
     9541 agtattttat ctttcaagtt catcacgcct ttaatgagta ttaagtacta tcaatgatag
     9601 cacaaaaaat tcaaagaata attgtataac acaatacagc catttaaatt tcgcaagatt
     9661 ttttgttgta atatgtaaaa aaaatagatt ataatcctta tagaccgatc gcacggtcta
     9721 taaggattgg agggaactta aatgatttca ttttttacaa aaactactga tatgatgaca
     9781 tcaaaaaaaa gatggactgc actagtagta ttagctgtta gtttgtttgt tgttacaatg
     9841 gatatgacaa tattaattat ggctttaccg gaattagtaa gagagttaga gccttctggt
     9901 acccaacagt tatggatagt tgatatatac tctcttgttt tagctggctt tataattcca
     9961 ttgagtgcct ttgctgataa atggggaaga aaaaaagcat tattaactgg atttgcttta
    10021 tttggcctcg tttcattagc tatatttttc gcagaaagtg cagagttcgt aatagctatt
    10081 cgatttttac ttggtattgc aggtgcttta ataatgccaa ctactctttc aatgataaga
    10141 gtaatttttg aaaaccctaa agaaagggcc actgcattag ctgtatggtc aatcgcttca
    10201 tcgataggtg ctgtttttgg accaattatc ggaggagctt tacttgagca attttcatgg
    10261 cactcggcat ttttaattaa tgtaccgttt gcgataatag cagttgtagc aggtttattt
    10321 ttattaccag agtctaagtt atcaaaagaa aagtctcact cgtgggatat tccttctaca
    10381 attttatcaa ttgcaggcat gattggactg gtatggagta tcaaagaatt ttcaaaagaa
    10441 ggactagcag atattattcc atgggttgta atagtattag caattaccat gatagtgata
    10501 tttgttaaac gtaatttatc aagttctgat ccaatgttag acgtaagact ttttaaaaag
    10561 agatcatttt cagctggtac aattgctgca tttatgacaa tgtttgcaat ggcatctgtt
    10621 ttgttattag cttcacaatg gttacaggtt gtggaagaac tttctccttt taaagctggc
    10681 ttatacctat tacctatggc aataggagat atggtgtttg caccaattgc acccggatta
    10741 gcggcgcgat ttggaccgaa aatagtgtta ccttccggaa ttggaattgc agccattggc
    10801 atgtttatta tgtatttctt tggtcatcca ttatcatatt ctacaatggc tttagcatta
    10861 attttagttg gagctggtat ggcttcacta gcagttgcat ctgctctaat aatgttagaa
    10921 acacctacat caaaagcagg taatgcagct gctgttgaag agtctatgta tgaccttgga
    10981 aatgtttttg gtgtagcagt acttggtagc ctatcttcta tgctttatcg tgtattttta
    11041 gatatttcat ctttttcatc aaaaggtata gttggagatt tagctcatgt agctgaagaa
    11101 tctgtagtgg gcgctgtcga agtagctaaa gctacgggga taaaacagct tgcaaacgag
    11161 gctgtaacat catttaatga tgcttttgta gcaactgctt tagtaagtgg gattatcatg
    11221 attatcattt caatagttgt ctatttgtta attcccaaat cacttgatat aactaaacaa
    11281 aaatagtact aaacatatat ttttagatat tgtagattag tttattttta aaattagtaa
    11341 tagttaaaaa gtagaaccat taattttaaa tggttctact tttttattat taagtaaaat
    11401 tataaaagac aatttcttaa attaataata tgatatacct atatctaata atttattagt
    11461 attaataaac cttgaaagga tgattaactg atgaatagag cagatatttt taaaatattt
    11521 gatgtagaac ttaatataac tatattttta ggagcactag tagcaacttt catctatttt
    11581 ataagttaca tgaacaatgt aaatgaatta attacattat gcttaacatt agcttttcct
    11641 gcatatttat taaaaattag cagatcgttc gaggttattt tattagagga aaaaataaaa
    11701 aatagtaaaa tcaaaatctg tgacaaagat agaattgaac gagagatact ggtgaaaaag
    11761 aacctaatag atgaactatt gtttcctaat agagttatta aaaaatatac actgacaatt
    11821 ttaatttaca gtataggtat tattattctt ttactacttt gtaatatgtt aagttatgga
    11881 attggtgaaa atgtcaattt tatctggaga ttactagctt gttatgtaac gagtgtcaca
    11941 gtgattatag ttgccttgat tataatgata atgatatttg atcttataat tggattacta
    12001 ttaaattttc aaatattttt aaaaaataaa aagtatgatg actttttata attattcgat
    12061 ttatataatg aaaaattaaa tgattaaaca tagaaacctc aaaacaaacc atatttaata
    12121 gagtttatgg cgccttatac aaataaaggc tatgctgtta aagaattatg tagctatctt
    12181 cagattaata tggatgatgt ttatactatt ggcgatagaa aattgattta cctatgttta
    12241 ataccctaga taattcttct gataatttag tttttgttag agtttaacat aactgatgat
    12301 tatcagaagt ttgattcaca ttaaaaaggc acaacaagtt catgaacttg tgtgcctttt
    12361 taattgcaat tagttaccaa ataaacgact ccaaaatctc tttttaggtg gttctacctc
    12421 cctattatca ctggaatcgt tcttcgtttt ttcaacaaac ttatcctctt ttgttaattc
    12481 atctgtactg tcatgcttat tctcttcagt tatattttta tgatttactt cggttggttg
    12541 acattctttt tgttgttggt tgatattttg agattcttca gtaaaagttg cttcttgtgg
    12601 attaatattc tgtctattat tcatcgttgt atcaaaagaa tatttaagct gtctttcttc
    12661 ttctaattgg ctttctagtt tttgaatttt tttattgttt tctaaagata atatttgttg
    12721 attctccaac agtttattta atgtattaac attgctattt tgttgatcta attgtttagt
    12781 tagattctca atatattttt catcattttt agctctagtt tcaaagattt ctatttgctt
    12841 ttctaattcg tttacttttg tttttaatgt ctcagaacta tttgaattat tatgtatata
    12901 cttttcaact tttgcagtat tttcattaaa taattcattt tttactgatt ctttattatg
    12961 agttgttttc tctttcttag tgattttttc tataattttt aaataatcat tatctttaat
    13021 ataattaatt ccattttctt tttcaaaaga tatatttaat cttttagcat tattaacaat
    13081 agtttgttta gttacaccta attcatcagc aatcatttta atagttttca taacatcccc
    13141 cttaggtacc taatttagta cctaaatttt accacctaac aaaatttatt tcatcattta
    13201 gtactaaatt tactatatat taactaccta aatgcctaat tatctatcct taaaatattt
    13261 tcgccactta attaggtgct aaaatagcga attatacgtt tggtagtttt aggtgtactt
    13321 ttaattacat ttaaaactct ttatatacgc cattaaaagt gttaatatta ctttataaat
    13381 attaaaagag tcgatgctat tggcgtagca tcgactctcg gtaataaaac gattcgcact
    13441 cgtttgttta tatatttttt tgatacttgt attatatata tctaatcatc taagtgcaag
    13501 cacaaaacat ataacttacg taaaaattgt tttattacct caatcccaaa atggaaatga
    13561 ggtttttatt
//
