LOCUS       CP052875                4995 bp    DNA     circular BCT 11-MAY-2021
DEFINITION  Enterobacter cloacae strain 3849 plasmid p3849II, complete sequence.
ACCESSION   CP052875
VERSION     CP052875.1
DBLINK      BioProject: PRJNA628587
            BioSample: SAMN14734641
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Marchetti,V.M., Bitar,I., Sarti,M., Fogato,E., Scaltriti,E.,
            Bracchi,C., Hrabak,J., Pongolini,S. and Migliavacca,R.
  TITLE     Genomic Characterization of VIM and MCR Co-Producers: The First Two
            Clinical Cases, in Italy
  JOURNAL   Diagnostics (Basel) 11 (1), 79 (2021)
   PUBMED   33418979
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly v. SMRT Link v.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 439.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2020 19:20:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,986
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,759
            CDSs (with protein)               :: 4,759
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 49 of 112
            Pseudo Genes (incomplete)         :: 68 of 112
            Pseudo Genes (internal stop)      :: 22 of 112
            Pseudo Genes (multiple problems)  :: 22 of 112
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="3849"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="p3849II"
                     /geo_loc_name="Italy: Milan"
                     /lat_lon="45.46 N 9.19 E"
                     /collection_date="07-Jul-2017"
                     /collected_by="LTCF Golgi Redaelli"
     gene            complement(join(4990..4995,1..210))
                     /gene="mbeD"
                     /locus_tag="HJI43_24895"
     CDS             complement(join(4990..4995,1..210))
                     /gene="mbeD"
                     /locus_tag="HJI43_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJP79032.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="mbeD"
     gene            complement(217..708)
                     /locus_tag="HJI43_24900"
     CDS             complement(217..708)
                     /locus_tag="HJI43_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJP79026.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="mobilization protein"
     gene            complement(683..1228)
                     /locus_tag="HJI43_24905"
                     /pseudo=""
     CDS             complement(683..1228)
                     /locus_tag="HJI43_24905"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1386..1730)
                     /locus_tag="HJI43_24910"
     CDS             complement(1386..1730)
                     /locus_tag="HJI43_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJP79027.1"
                     /translation="MSEKRSKMLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPAR
                     SGKLPSISPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2839..3147
                     /locus_tag="HJI43_24915"
     CDS             2839..3147
                     /locus_tag="HJI43_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="QJP79028.1"
                     /translation="MKNSFELISTDPVKFNTWSIKSKLIIIIRKLMIDNKWSQSEAAEK
                     LGISQPRVSNMLNGKLDKFSVDTLLEMVFKMGYKLDMDFTPLNTESPLTMVVKKAMV"
                     /name="XRE family transcriptional regulator"
     gene            complement(3163..3495)
                     /locus_tag="HJI43_24920"
     CDS             complement(3163..3495)
                     /locus_tag="HJI43_24920"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJP79029.1"
                     /translation="MSNDENEYTFKPVNAQAIKALKSLPEWVQAAFVHALDSVFYGVDP
                     GMSIAHLTVIGPGVVELRINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMATTA
                     SRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3937..4140
                     /locus_tag="HJI43_24925"
     CDS             3937..4140
                     /locus_tag="HJI43_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017191052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJP79030.1"
                     /translation="MAIYVGPHSKVGRNGGSFQECDINTTNHTVTRKYGRYCTIRDNEY
                     APPTSVPGRHWLQIFRTPDNTE"
                     /name="hypothetical protein"
     gene            4276..4503
                     /locus_tag="HJI43_24930"
     CDS             4276..4503
                     /locus_tag="HJI43_24930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJP79031.1"
                     /translation="MGYHYKELTVIELTAEEFELHKEGYSLELLTNMRNEEHTKYWEVC
                     PSCEGKMDPEENQGHEGYCATCWLDNPDWW"
                     /name="hypothetical protein"
     Typing          complement(1823..1996)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          1963..2489
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1396)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.146"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cccggacaag cggcgcagct gctcgcttaa actcagcacc tgcgtgctca agtccgacac
       61 gcgctcgttc agcatcgcgt tctcccgttg cataagccca cacatgctcc gccattccgc
      121 gaaggcgctc tcccattcgt ccagcctttg cgagtagtcc tgctgtagct gctctaatgc
      181 gctcagcaac tgcttttcca gctctgtcat tttccgctac tcctgtttca ggcggatcca
      241 tgtcgtcccg tcgtagggga tggcttcggt ttgcatcccc ggcggcagga taagaaacct
      301 ccggccgtcg ctgctctcct gatagcgcac tccccacgtc cgggcggtca ttttcgccag
      361 ggtatcttcc tgctgacgaa gatgcgtgta attgtcggtt atctgctgcc cctgccacca
      421 cagaatgctc ccgctcgtgc cgatcagcag cgctgacacc atcaggatgg tgagccatgt
      481 ccgcccggca gtccgcagta cgctccgccg gtttttctcc atcgcctcgc tcatactctg
      541 ctcgtgggcg aggatggcgt cgctgattct cctcgcgctt gcgctcagtt ccgctctgac
      601 agactgctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct gctgctgcgc
      661 tttcgattgc tgctctaagt ccttcgccag cgttaaaaga ctgctcatag atggctccct
      721 tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatactgctt ttcgtggtgc
      781 gcaccacctc aaaacctgca ccttcaagag tgtcagtgac atcctgacgg cttttcagct
      841 ccccggacga agcaagggtc agcagtcccc gggtaatggc ttctgcggcc tcctgcttcg
      901 ttttcggcag cccggacggc gtgaccagcg cccggcggtt ttccggcgcg ttcgggtcgt
      961 gcagccccag tctgccgttc acgatagtct gccaggcatc gatgcgcggc cggtcggcgc
     1021 ggtcgtagta aggctggagc cgcctgccgg tcagcagctc ggtattgggg atcagaaagt
     1081 tcagctccag acgccccttg tccgcgtgct ccacccacag aatgctgtac tgatctttat
     1141 cgagtccggg catcagaacc cgctcgaagc tggctatcag tttttcccgc tggccgggag
     1201 gcagatcggc ctccgcaaac gacagaacgc cggaggtgta tttcttaacg taaggcgacg
     1261 cgtcaatcag ctcccggact tcttccggct tcccctgcag gacggtggcg ccttcacgct
     1321 gccgatcttt acccagcaga tagtccaccg gcccggcgcc accgccgcgg ccgcgcggat
     1381 gaaatttaac gatcatcgtc cgttcctttc tcaagaacgg cgtgccgcag ccgctcaagg
     1441 ccggcatcga tggccatcag cgccgccaca atctgtaccc tgtcatggcc ggtgccgccg
     1501 ccggcgttaa cacggcgggc tatctggttc aggttattgc ccatgccggc aagctggcgc
     1561 agcagcgccg gcgagatcga ggggagcttc ccggaacgtg ccggcttttc gtccaggcag
     1621 atctgccgca tccacgccgc cagctgcctg ccgtcacagc gctcgagcag ccgccggtgc
     1681 tcgtcctccg tcacccacat ggtgagcatc ttgctccgtt tctctgacaa accggcctcc
     1741 tgataacgcc gggacgggcg ggaaagcctt tcccggtcgg cgcggggttg gacaagccgc
     1801 aggcgcgtca ggcttttggc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta
     1861 gcggagtgta tactggctta ctatgttggc acgaatgcgg atgtcagtga agtgcttcat
     1921 gtggaaggag aaaaaatacg gcaccggcgc gtcagcagaa tatgcgatac aggatatatt
     1981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggaaatgg
     2041 cttactggcg gggcggaaat gtcctggaag atgccaggaa gaaacttagc agggaagcaa
     2101 taaggccgcg gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcacaaaat
     2161 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2221 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcactcc
     2281 gctgttatgg ccgcgtttat ctcattccac gcctgacact cggttccggg aaggcagttc
     2341 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gcctgttccg
     2401 gtaactatcg acttgagtcc aacccggaaa agcacgactt aacgccactg gcggcagcca
     2461 ctggtaaagg gtgtacaaga gataactgat gcagagttct tgaagtatca gtccaactac
     2521 ggctaaactg gaaggacgaa ttaggtggca gcggtcttgt acaccctgtt accacggttc
     2581 ataaacttgc cggtttaatg aaccttcgaa aaaccacctt gccgggtggt tttttcgttt
     2641 atgggcaaag actactcttt gctggattga tacgtcaaca caataagctt tacttaaaag
     2701 atcaaaatgt ctttacactt tttcctcttg aaagcaatat cattacttgt ataatgcccc
     2761 gcccgtatat atatataagg agttccttgc cggaactttt atcattgcga tacaggtaat
     2821 ccgaagtgga gctgttctat gaaaaattca tttgagctga tcagtacaga cccagtaaag
     2881 ttcaatacat ggtcgatcaa gtcaaaactc attatcataa ttagaaaact aatgattgat
     2941 aacaaatggt cgcaatcaga agcggcagaa aagttaggta tcagtcaacc aagagtaagc
     3001 aatatgctga acggaaaact ggacaaattc tccgtcgata cgctgttgga aatggttttc
     3061 aagatggggt acaagctgga tatggacttc actccattaa acacagagtc gcccctgact
     3121 atggttgtaa aaaaggcgat ggtgtaacca tcgccaattt gattagagca acttgtagcg
     3181 cgaagctgtg gttgccatgt ttttcttatc tggtccttgg cacgttttca caaacgcatg
     3241 cagtaagtcc acctggccgg gtgccttact gcggtataca cagcgataag ctgggcgacc
     3301 attgattctc aactcaacaa cgccggggcc gatgaccgtc aagtgtgcga tggacatccc
     3361 cggatccacc ccgtagaaca ccgaatccaa tgcgtgcacg aatgcagcct gaacccattc
     3421 tgggagtgat tttaatgcct tgatagcctg ggcattaaca ggtttgaacg tgtattcatt
     3481 ttcgtcgttt gacataaaca tcctcatcta ggttattgat ttttagcaac ttaactattg
     3541 ttaaaacgtt tcgcatataa actccgattg attgattgat ttatgagcat aaaaaaacca
     3601 caccgcccta tatttaggaa agtgcgcgca tggttattgc tcgttgaacc aacaattcat
     3661 tggcaatctc gccaacgatg ccatagtata taaattctta tatgtcatca attcttatta
     3721 gtgcttcaaa cgtctgtttg aataaaaaaa aatcaatttg acttaggcgc agaagaccta
     3781 acagttacat gtgttggttt tccttacatc aagatatgag gattttatcg acgcaatgtg
     3841 gaaagccaac aaagcacact atgatattga taaatataga aattaataaa atctacttag
     3901 atactggtaa tcaactatct gacgaaggag cttagaatgg ctatttatgt tggtcctcac
     3961 agcaaagtag gtcgtaatgg tggttcattc caggaatgtg atatcaacac aacgaatcat
     4021 acggtaaccc gaaaatatgg ccgctactgc acaatccgcg ataatgagta cgcccctcca
     4081 accagcgttc caggccgcca ttggctgcaa attttccgca cgccagataa cacagagtaa
     4141 ttcatcttct gcttagtaaa gcggtaatat cccttgtata aaccggatag gattatcatt
     4201 gtaagatact gaaaacactg acatttaatg cttttcagta tcttagtatt gagctgcata
     4261 aagcgaggca agcgaatggg ttaccattac aaggaattaa ccgtcatcga attaactgcg
     4321 gaagagtttg agcttcataa agagggatac tctttagagc ttttaactaa catgagaaat
     4381 gaagaacaca ccaagtattg ggaagtttgc ccttcctgcg aaggaaaaat ggatccagaa
     4441 gaaaatcaag ggcatgaagg ttactgtgcc acatgctggc tcgataaccc tgactggtgg
     4501 tagttaataa tttaccataa catccgttgc ccgcaccgct gaaacggggt tggctgcgtt
     4561 tccggtgcca gacgcgagaa agtggcgaca gacataacga aaaaacacca gaaacgcagc
     4621 cgctgaatat tacactggtg catagtcatg cagccagatg catggatgtt aaagtcacac
     4681 taaaatccgc gccaatgcct taaaaaccga caaatgcttc tccagccagt cccacagctc
     4741 cgccgccaga tacaggcagt taagcaatga ttcaataaga tttaacagct tcatttcccg
     4801 ctcctgaata agtcgtgacc tttttgatca cgaacctatt tagcgggaga aataagagcc
     4861 cctcttcgtg cgggttgtca gctgcctgga tggtttaaag tgtcggcccg cggtaggttt
     4921 tctgcggctg gcgctccagc tccagtgttt tctgatactc attctccctc tgccggagct
     4981 cgatggcatt cagtt
//
