LOCUS       CP052874                4667 bp    DNA     circular BCT 11-MAY-2021
DEFINITION  Enterobacter cloacae strain 3849 plasmid p3849I, complete sequence.
ACCESSION   CP052874
VERSION     CP052874.1
DBLINK      BioProject: PRJNA628587
            BioSample: SAMN14734641
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Marchetti,V.M., Bitar,I., Sarti,M., Fogato,E., Scaltriti,E.,
            Bracchi,C., Hrabak,J., Pongolini,S. and Migliavacca,R.
  TITLE     Genomic Characterization of VIM and MCR Co-Producers: The First Two
            Clinical Cases, in Italy
  JOURNAL   Diagnostics (Basel) 11 (1), 79 (2021)
   PUBMED   33418979
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly v. SMRT Link v.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 439.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/29/2020 19:20:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,986
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,759
            CDSs (with protein)               :: 4,759
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 49 of 112
            Pseudo Genes (incomplete)         :: 68 of 112
            Pseudo Genes (internal stop)      :: 22 of 112
            Pseudo Genes (multiple problems)  :: 22 of 112
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="3849"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="p3849I"
                     /geo_loc_name="Italy: Milan"
                     /lat_lon="45.46 N 9.19 E"
                     /collection_date="07-Jul-2017"
                     /collected_by="LTCF Golgi Redaelli"
     gene            complement(229..573)
                     /locus_tag="HJI43_24870"
     CDS             complement(229..573)
                     /locus_tag="HJI43_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJP79022.1"
                     /translation="MSEKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPAR
                     SGKLPSISPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1612..3183)
                     /locus_tag="HJI43_24875"
     CDS             complement(1612..3183)
                     /locus_tag="HJI43_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="QJP79023.1"
                     /translation="MECIFMRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIE
                     KDLLDANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMF
                     NSVFVVSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLS
                     HPIYDKNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAV
                     GRPMVMSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILI
                     TYAKSVLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYA
                     EADRLKKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAI
                     DVDFFKKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAE
                     CVAERIRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKV
                     IVYSAI"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(3514..3729)
                     /gene="mbeD"
                     /locus_tag="HJI43_24880"
     CDS             complement(3514..3729)
                     /gene="mbeD"
                     /locus_tag="HJI43_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJP79024.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="mbeD"
     gene            complement(3733..4218)
                     /locus_tag="HJI43_24885"
     CDS             complement(3733..4218)
                     /locus_tag="HJI43_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJP79025.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="mobilization protein"
     gene            complement(4193..4663)
                     /locus_tag="HJI43_24890"
                     /pseudo=""
     CDS             complement(4193..4663)
                     /locus_tag="HJI43_24890"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(666..838)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="100.0"
                     /evalue="9.27e-76"
     Typing          763..1315
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.46"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(4..239)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.734"
                     /coverage="97.0"
                     /evalue="2.16e-42"
     Typing          complement(236..552)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          complement(3411..4666)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.169"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acccgctcaa agcttgccat cagcttttcc cgctggccgg agggtaaatc cgcttccgca
       61 aacgacagta ccccggaggt gtatttctta acgtaaggcg acgcgtcaat cagctcccgg
      121 acttcttccg gcttcccctg caggacggtg gcgccttcac gctgccggtc tttacccagc
      181 agatagtcca ccggcccggc gccaccgccg cgaccgcgtg gatgaaattt aacgatcatc
      241 gtccgttcct ttctcaagaa cggcgtgccg cagccgctca agtccggcat cgatggccat
      301 cagcgccgca acaatctgca ccctgtcatg gccggtaccg ccgccggcgt taacacggcg
      361 ggctatctgg ttcaggttat tgcccatgcc ggcaagctgg cgcagcagcg ccggcgagat
      421 tgagggaagc tttccggaac gtgccggctt ttcgtccagg caggtctgcc gcatccacgc
      481 tgccagctgc ctgccgtcgc agcgctcaag cagccgccgg tgctcgtcct ccgtcaccca
      541 catggtgagc atcttgttcc gtttctctga caaaccggcc tcctgataac gccgggacgg
      601 gcgggaaagc ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg tcaggctttt
      661 ggcgggtttc ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc
      721 ttactatgtt ggcacggatg cgagtgtcag tgaaatgctt catgcggcag gagaaaaatg
      781 cagcagcgga gcgtcagcag aatatgtgat acaagatata ttccgcttcc tcgctcactg
      841 actcgctgcg ctcggtcgtt cggctgcggc gagcggtacc ggcttacagg cggggcggaa
      901 atttcctggg agatgccagg aagaagctta acagggaagc gataaggcca cggcgaagcc
      961 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     1021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggtg gctccctcgt
     1081 gcgctctcct gttcctgcct ttcggtttac cggtgtcaat ccgctgttat ggccgcgttt
     1141 atctcattcc acgcctgaca ctcggttccg ggaaggcagt tcgctccaag ctggactgta
     1201 tgcacgaacc ccccgttcag cccgactacc acgttgtccc ggtaactatc atcttgagtc
     1261 caacccggaa cagacacgaa aaatcgccag tggcggtagc cattagtaag aggattcacg
     1321 gagcggagta gtagagtgtt ctacagagtt cttgaagtgg tggcctgact gcggctacac
     1381 tggaaggaca gttttggtac ctgcgctccg tgaaagccag ttaccttcgg aagaaagagt
     1441 tggtagctct tgattccggc aaacgaacca ccgttggtag cggtggtttt tttgtttgca
     1501 gggcatgaga ttacgacgac atcgaaggga taacaagagg atccgcttct tgaacccccc
     1561 tccccacaaa aaaaaacggc acactagcgc gtaaagcgtc atcaaaaaaa ctcaaatagc
     1621 gctatataca atcactttat tacgcccctc tctcttagct tgataaagag catcgtcagc
     1681 atttttcagc aaaaaatcga tcccaccaag aggatcatct tgtgttgcca caccagcaga
     1741 tacggtgaca cgcaagttat tagggaaaac agtacgttca ataacactcc taatacgctc
     1801 cgcaacacac tccgctgtta ccacagatgt attcggcaga aaaacaacaa actcttctcc
     1861 accaaaccga caaataatat cttcactacg gcaacaagac ttaataatct gagccaaaga
     1921 aacaagcacc tcatctccga ccccatgccc gaaactatca tttatttttt taaaaaaatc
     1981 aacatcaata gcaataactg aagctcctgc cccatacttg ttattctgta ttttagttga
     2041 aaaccccaat ctattgttca cgccggtaag gggatcttta agcgccacct catttaaaga
     2101 atcaaccctt tccatcatta ccttaacatg cgtaaaaagt gcctttttaa gtcgatcagc
     2161 ttcggcatac caagcattga tagctttaat ataaactaac gaagccccta tatctttagc
     2221 attggtagat atagcaagct tttctaaagg aagtgaaata cgggaggcaa tgaaatatga
     2281 aagaagtgaa aatgtaataa ttattacaat tagcagaaca agtacacttt tagcataagt
     2341 aataagaata gtagtcgcgc tgtcagcatg gctataaacc aacacattcc aagcagtatt
     2401 tttcatatga gcataaccca gaagatatct attccccgaa aaaaaaaatt ccccctcacc
     2461 tctatcagac tccgttaact ttgatttcaa atcatcagac ataaccatgg gccgaccaac
     2521 agcactgtta tcgttattaa agatcacagt tccatcatca ctaacaatgc taatttcagt
     2581 gtcgctcttg aaaaaatgcc tgctcaaaac atcgctaaat aaactatgtt tttttagata
     2641 tatagaccca ccgacatgcc ctatgtaatt gccattctta tcataaatag gatgtgacaa
     2701 caaaataatc aaattcccag taactgattt gaacggtgaa gatatgaatg gtttcttttc
     2761 ctcaatcgcc aatttgctgg cacttgaatt caagcgggta ccaactaaat caagagattc
     2821 tggcgaagtc gccagaacaa cagcatcatt actaacaaca aaaactgaat taaacatacc
     2881 agactgcaat cttaacctat ccgcctcatc tttaagtgag tcaatatcat tagttgattt
     2941 tatttttttt gctccatatg ctaattcacc ttgagccatt tcaatataac cactcattac
     3001 atctgacatt ttcatagcgt acgatgaatt agcatcaagc aaatcttttt ctatattttc
     3061 gcgctgaaaa accataataa cggcaatcag aaaaatagaa gttaacaaaa tcccaccaac
     3121 ggacaaggtt atgactatcg gacgaaggtg aggcttttta attctcataa aaatacactc
     3181 catataataa aaaatgccgc atttaccata acatccctta tcagcaccgc cccggacggg
     3241 ttcggaacaa atgcggcgtc agcccggtaa aaccggcagc aaactctccg ctaaaccgcg
     3301 cattctgcgc cagataaatt tcacccggct gcatggctat gcagtcgggt gaatggctcc
     3361 ggctttcagc gcagatgtcc accgaaagcc ccgttttatc aggttatcac agtgaagggc
     3421 tgttatacgt ccgctgtcgc tgagatcgct ccagctccag cgctttctga tgctgcacca
     3481 cgcgctcctg ctcccggtgc agctcgacgg cattcagtcg ctcgacaacc ggcgcagctg
     3541 ctcgctcaaa ctctgcacct gcctgctcaa gcccgtgacg cgctcgctca gcgctgcgtt
     3601 ctcccgttgc ataagaccag acatcgtccg ccattccgcg aaggcgctct cccactcgtc
     3661 cagccttttc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact gtttttccag
     3721 ctccgtcatg tgctatttcc ccgccagtat catccagctc gaatcctctc cgaaccgtcc
     3781 cgcttccggg ttcaccttca cgcagcggcg tctctgctcg ccgcaggtcg agagctgcac
     3841 gccgctgttc ctctctgaca gcatggcctg cgtgctcttc tgctcccgga tggtcgtgta
     3901 gttgtcgagt atctgctgcc cctgccacca cagaatgccc gcgctcgagg cgatcagcag
     3961 cgcggagacc aggacgatgg tcagccacgt ctggctgacc atgcgcacca tgcctttcgt
     4021 gcgctggctc atggctgagg acagtttccg gtcgtggtcg aggatggcgg cgctgattct
     4081 cttcgcgctt tcgttcagtt ccgcttttac agatttctcg tgctcgctga atgcggcttt
     4141 cagcatctcg ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag
     4201 cgttaaaaga ctgttcataa atggctccct tcagtcggat gtttcgcccc ccgtccgggt
     4261 cggcaatgct gatactgctt tttgtggtgc ggaccacctc aaaacctgcg ctttccagcg
     4321 cctcagtgac atcctgacgg gttttcagct ccccggatga ggcaagagca agcagaccgc
     4381 gtgtaatcgc ttctgcagcc tcctgcttcg ctttaggcag tgcagacggt gtgaccagtg
     4441 cccgccggtt ctccggtgcg ttcgggtcgt gcagccccag tctgccgttc acgatggtct
     4501 gccaggcatc gatgcgtgga cggtcggcgc gatcgtaata cggctggagc cgtctgccgg
     4561 tcagcagctc cgtgttcggg atcagaaaat tcagctcaag ccgccccttg tcacgatgct
     4621 ccacccacag gatgctgtac tggtctttat caagtccggg catcaga
//
