LOCUS       CP052719                3514 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0048 plasmid pB16KP0048-3,
            complete sequence.
ACCESSION   CP052719
VERSION     CP052719.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609865
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:42:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,510
            CDSs (total)                      :: 5,383
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 58 of 106
            Pseudo Genes (incomplete)         :: 42 of 106
            Pseudo Genes (internal stop)      :: 23 of 106
            Pseudo Genes (multiple problems)  :: 16 of 106
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0048"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0048-3"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            1002..1343
                     /locus_tag="HJW77_27470"
     CDS             1002..1343
                     /locus_tag="HJW77_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJN68688.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     QEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1282..2040
                     /locus_tag="HJW77_27475"
                     /pseudo=""
     CDS             1282..2040
                     /locus_tag="HJW77_27475"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2021..2518
                     /locus_tag="HJW77_27480"
     CDS             2021..2518
                     /locus_tag="HJW77_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJN68685.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="mobilization protein"
     gene            2525..2740
                     /gene="mbeD"
                     /locus_tag="HJW77_27485"
     CDS             2525..2740
                     /gene="mbeD"
                     /locus_tag="HJW77_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJN68686.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="mbeD"
     gene            complement(3154..3345)
                     /locus_tag="HJW77_27490"
     CDS             complement(3154..3345)
                     /locus_tag="HJW77_27490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN68687.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLTK"
                     /name="hypothetical protein"
     Typing          745..917
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          complement(274..820)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          1334..2856
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaactacgcc aaaactttga atgatcgggt ggcaggctaa atgaaatctt gagattcatt
       61 cagcctcgtc gtaatctctt gctctgtaaa cgaaaaaacc gccttgcagg gcggtttttc
      121 gaaggttaag tcagttgggg aactgcttaa ccaggtaact ggcttcagca gagcgcagat
      181 accaaatact gtccttccag tgtagccgta gttaggccac cacttcaaga actctgtagc
      241 atcacctaca ttaactcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgctt
      301 tggcgtgtct ttccgggttg gactcaagac gatagttacc ggataaggcg cagcagtcgg
      361 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaactga
      421 gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga
      481 aaggcaggaa caggagagcg cacgagggag ctaccagggg gaaacgcctg ggatctttat
      541 agtcctgtcg ggtttcgccg ccactgattt gagcgtcaga ttttgtgatg cttgtcaggg
      601 gggcggagcc tatggaaaaa cggcttcgcc gcggcctcat cgcttccctg ttaagtttct
      661 tcctggtatc ttcctggaaa tttccgcccc gcccgtaagc cggcaccgct cgccgcagcc
      721 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga atatcttctg tatcgcatat
      781 tctgcttacg ctctgctgca gcatttttct tctgccgcat aaggcgcttt cctgattatc
      841 gcactcatgc caacatacta agccagtata cactccgcta gcgctacgtg actggttcag
      901 ggctgcgccc cgaaacccca ctgacgcgcc tgcggcttgt ccaaccccgc gccgaccggg
      961 aaaggctttc ccgtccgtca cggcgttatc aggaggcgat tctggcagat aaacgcaaca
     1021 agatgctcac gatgtgggtg acggaagacg aacaccggcg gttgcttgag cggtgcgacg
     1081 gaaaacaact ggcggcgtgg atgcgccaga cctgccttga tgagaaaccg acacgcgccg
     1141 gcaggctccc ctcgatttcg ccggcgctgc tgcgtcagct tgccggcatg ggcaataacc
     1201 tgaaccagat cgcccggaaa gtgaacgccg gtggtgccgg tcatgaccgg gtgcagatcg
     1261 tggcagcgct gatggcgatc gatgccggtc tggaacggtt gcggcatgcc gtacaggaaa
     1321 aagggccgga cgatgattgt taaattccat ccccggggtc gcggcggggg cgccggtccg
     1381 gtggactatc ttctgggtaa agaccggcag cgtgaaggtg ccagcgtcct gcaggggaag
     1441 ccggaagaag tccgggagct gattgatgcg tcgccgtatg tgaaaaaata tacctccggg
     1501 gtgctgtcat tcgcggaggc ggacctgccg ccgggtcagc gtgaaaaact gatggccagc
     1561 tttgagcggg ttctgatgcc cggactcgat aaagatcagt acagcattct gtgggtggaa
     1621 cacgcagaca agggtcgtct tgagctgaat tttctgatcc ccaataccga attactgacg
     1681 gggaaacgcc tccagccgta ttacgatcgt gccgaccggc cgcgcatcga tgcgtggcag
     1741 acagtggtca acggcaggct ggggctgcat gatccgaacg acccggagaa ccggcgggcg
     1801 ctggtcacgc cgtcaggact gccagaaacg aagcaggaag ccgcggagac gattacacgg
     1861 ggtttgctga tgttagcctc atcaggagag ctgaaaacgc gtgatgacgt cacaggggcg
     1921 ctcacagcgg ccggttttga ggtggtcaga accacaaaaa acagtatcag cattgccgac
     1981 ccggacgggg ggcgaaatat ccgactgagg ggagccattt atgagcagtc ttttaacggc
     2041 ctcgagggac ttcgaggaga aatcgaaagc acagcagcag cttaccgaag agaggctgaa
     2101 agccgcattc agcaagcacg agaacgttgt caaatcggaa ctgaacgcaa gcgcgaagag
     2161 aatcaacgac gccatcagcg cccacgaaca ggggatgaac gcggcgatgc agtcgaaccg
     2221 gctgagcgtg ctgcgaatgg tcgggcggac gtggctgacc atcaccatgg tgtcagggct
     2281 gctgttcgcc agcctgagcg gggtgctgtg gtaccagggg aaccggatag cggcgaacct
     2341 ggcggaaatc aggcagcaga gagatacgct gtcgaaactg cacatgcaga cgtggggcgt
     2401 gacctatctg gagaaccgca acggacggtt tctggtgctg ccggagggga tgaaagccga
     2461 aacgggccgg acggtggaca acaagacgcg gaacgcggtg aagctggtac gggagtagag
     2521 cagaatgacg gaactggaaa aacaattgct gagcgcgtta gagcagctac agcaggacta
     2581 ctcgagaagg ctggacgagt gggagagcgc cttcgaggaa tggcggaaga tgtctggtct
     2641 tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgtcg ggcttgagca ggcaggtgca
     2701 gagtttgagc gagcagctgc gccggttatc gacaggctga atgagattga gcaacagcga
     2761 tacgggcaaa caacggaaaa ggaaccagag aagacagcag atctggtacc ggaacggcaa
     2821 cgcacattat ctcatggtga cagggagtat ggagactgat aaacgcccgt ctcaggtggc
     2881 ctgagcgtga cggggatcat ggccattttc tgcgatttca gcacacagag agtctttttt
     2941 aaccgaaatc accggctatg cagtgatgtg aataagtgat gcatatgcgt aaaataaatc
     3001 aggtgcgttt ctggtggttt tcggtgtact tgccaccacc ttttgcccgt cagccatcag
     3061 aaacgccctg agtgcctccg gcgcggtgcg ggcaagaaga cgttatggta aatcggtggc
     3121 tttatttcga tccttcatgc ttcaggggct gtatcacttt gttagtttta gttttgtggt
     3181 ttcggcttgg attccgagaa gatcgtggag gttattgttg atagcaaatt ttatatcttc
     3241 aaaacttgca tcaatcatga gtccctcatc cacacctcgt aaaaaaattt ttgtttttga
     3301 gttaccacat gattttatta agataatttc attttttttt aacatgacat tgtcattgtt
     3361 tgttgtcgtc agatgaatca ttatcgttgt ttttgtgtgt gtttgttaag gctgctaaga
     3421 ttattcctaa ccactgattt tgtacatatc atcaaaaaaa taaaaaaact aatgccctgt
     3481 ggtgggtatg taacaaatca cggggagcgg gtca
//
