LOCUS       CP052718                5126 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0048 plasmid pB16KP0048-2,
            complete sequence.
ACCESSION   CP052718
VERSION     CP052718.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609865
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:42:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,510
            CDSs (total)                      :: 5,383
            Genes (coding)                    :: 5,277
            CDSs (with protein)               :: 5,277
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 58 of 106
            Pseudo Genes (incomplete)         :: 42 of 106
            Pseudo Genes (internal stop)      :: 23 of 106
            Pseudo Genes (multiple problems)  :: 16 of 106
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0048"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0048-2"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(5069..5126,1..1634)
                     /locus_tag="HJW77_27440"
     CDS             join(5069..5126,1..1634)
                     /locus_tag="HJW77_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="QJN68681.1"
                     /translation="MSERYRAAVDAAAIFSETDKSGNITYVNEQFCDISGYSAQELIGQ
                     NHRILNSGYHPPEFFIDMWKKISRGQVWKGEICNRKKDGSLYWAESTIVPMYDDASQRV
                     QKYVSIRFDVTEKRKFLETLQWQAEHDELTGLPNRFLLSKALDQAIVKAKSQPSTVAVG
                     VLDLDGFKQINDGYGHEMGDRLLVAVADRLKHSMHIEDTVARFGGDEFVLILGVRDPKV
                     LESALQRLLAALSAVYIIDGIDINISASIGVTLYPNDNENADTLLRHADQAMYKAKQSG
                     RNCFHLFDVSKDKMDKSAFDTVIRVRQALHDGELCLHYQPKISLSSGAVIGFEALLRWQ
                     HPRDGLILPQYFIPLIEQSDLIVEIGEWVIDQALSQIEQWADLGHSWSVSVNIAALHLK
                     KENFVKSLKFLLDSHPNVLPQMLDIEITESVVIEHLPHVTQCLIACQDLGVTFSLDDFG
                     TGYSSLSYLKQLPTQSIKIDKSFIRDILIDKDSLRLTKAIIGLAKSFNREVIAEGVETV
                     EHEVLLMNLGCDVAQGYGIANPMPVEKVLSWVAKFVPAHLSRGGNR"
                     /name="EAL domain-containing protein"
     gene            complement(1611..2114)
                     /locus_tag="HJW77_27445"
     CDS             complement(1611..2114)
                     /locus_tag="HJW77_27445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN68680.1"
                     /translation="MPGFNYGGNSVGDGTNWSSERGSDPTTPGGGDNGHAGDHSSSGSS
                     SSTVEHTPWGDLTTDAAGYAYMNGIKLTADNSQLAYVSPTHLVRVLNSLLDRSYMKPEP
                     GEHSGVTGVPATSPSRVTLADNALKNAQSKAQDAAAALSKANAAISQAQSVRGRCGLSI
                     TPSR"
                     /name="hypothetical protein"
     gene            2400..2858
                     /locus_tag="HJW77_27450"
     CDS             2400..2858
                     /locus_tag="HJW77_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJN68682.1"
                     /translation="MAWHTVKDTLKLTGRSRSQLYRDMKKGLVSYRTGKDGRREFDTSE
                     LIRAYGELRTEETVKRHKKGQQEIDDTPAEHPQLKEIQQQLTALQQTVTLMLEDKSTRE
                     AELRQHDEERTQLLAEISRLNEDLEQEKKRSLWSRLFRRDIEEKNREK"
                     /name="entry exclusion protein 1"
     gene            complement(3204..3440)
                     /locus_tag="HJW77_27455"
     CDS             complement(3204..3440)
                     /locus_tag="HJW77_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN68683.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(4546..4656)
                     /locus_tag="HJW77_27460"
     CDS             complement(4546..4656)
                     /locus_tag="HJW77_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJN68684.1"
                     /translation="MILAACQVNNVRDTGGGTVSPTSTVTGVTLDSGTNR"
                     /name="colicin release lysis protein"
     gene            4676..5032
                     /locus_tag="HJW77_27465"
                     /pseudo=""
     CDS             4676..5032
                     /locus_tag="HJW77_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754514.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          complement(3676..3849)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.143"
                     /coverage="100.0"
                     /evalue="2.63e-66"
     Typing          3816..4336
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.702"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aatccggaaa tatcacctat gtcaatgaac agttctgtga tatttctggc tatagtgcac
       61 aggagttgat tgggcaaaac caccgcatac taaactcagg gtaccatccc cctgagttct
      121 ttattgatat gtggaaaaaa atttcacgtg ggcaggtatg gaaaggtgaa atttgcaacc
      181 gtaaaaagga tggcagcctg tattgggcag agagcaccat tgtacccatg tacgatgatg
      241 ccagccagcg ggtgcagaaa tacgtttcca ttcgtttcga tgtgaccgaa aagcggaagt
      301 ttctggaaac cttgcaatgg caggctgagc acgacgagct gacgggtctt ccaaatcgtt
      361 ttttgctgtc caaagcgctt gatcaggcta ttgtcaaagc aaagagccag cccagcacgg
      421 ttgccgtggg cgtgcttgat ctcgatgggt ttaagcagat taacgacggc tatggccacg
      481 agatgggtga tcgtctgtta gtggcggtgg ctgatcgcct gaaacatagt atgcatatcg
      541 aagataccgt agcccgtttt ggaggcgatg agttcgtgct gattttgggt gtgcgggatc
      601 ccaaggtgtt ggaaagcgcc ctgcagaggc ttttagctgc cctttccgcc gtctacatca
      661 tcgatggcat agacattaat atttccgcca gtatcggtgt gacgctctat ccaaacgata
      721 atgagaatgc cgatacactg ctacgtcatg ccgatcaggc tatgtataag gccaaacaaa
      781 gtggccgtaa ctgtttccat ttgtttgatg tatcaaaaga taagatggat aaatcagctt
      841 ttgataccgt aatacgggtt cgccaagcgt tacatgatgg tgagttatgc ttgcattatc
      901 aaccaaaaat aagtttgagc agtggggcag tgattggttt tgaagcacta ttgcgttggc
      961 agcatccgcg agatggactt atattaccgc agtattttat accgttgatt gagcagagcg
     1021 atctgatcgt ggagatcggc gagtgggtca tagatcaggc gctcagtcaa atagaacaat
     1081 gggcggactt gggtcattcg tggtctgtga gtgtaaacat tgcagcatta catttgaaaa
     1141 aagaaaactt tgtaaagtcc ctaaagtttt tgctggacag ccaccctaat gttttgccgc
     1201 agatgttgga tattgagatt accgaatcag tggtgataga acacttacca catgtcactc
     1261 agtgcctcat tgcttgccag gatctgggtg tgacattttc gttggacgac tttggaaccg
     1321 ggtattcatc gctgagctac cttaaacaac tgcccacgca gtcaatcaag atagacaaat
     1381 cttttatacg cgacatactc atcgataaag acagcttgag gctgactaag gcgatcatcg
     1441 gcctagcgaa atcgtttaac cgcgaggtta ttgctgaagg ggtggaaacg gttgagcacg
     1501 aagtgttgct gatgaaccta ggttgtgatg tagcgcaagg ctatggtatt gcgaacccca
     1561 tgccggtaga aaaagtgcta tcttgggtgg cgaagtttgt ccctgctcat ttatctagag
     1621 ggggtaatag ataaaccgca ccgtccacga actgattgcg cctgactaat agccgcattt
     1681 gctttcgaca gagcagctgc ggcatcttgg gcttttgatt gtgcattctt cagcgcgtta
     1741 tctgcgaggg ttacacggct tggggatgtc gcaggcaccc ccgtcacacc ggaatgttca
     1801 cctggctcgg gtttcatgta agaacgatcg agcagagaat taagtactcg cacaagatga
     1861 gttggagaaa cgtaagcaag ttgcgagtta tctgccgtca gtttaatacc attcatataa
     1921 gcatagccgg cagcgtcggt tgtcaggtct ccccaagggg tatgttcaac agttgatgac
     1981 gaactaccag atgaagaatg atctccagca tgaccattat ccccgccccc tggcgtagtg
     2041 gggtcagaac cccgctcaga actccaatta gtaccatccc ctacactgtt accaccataa
     2101 ttaaacccag gcataatttt tccctttttg ttttattttt attagaaatc aggtgtgcgg
     2161 tttgttgcct gccgtttccg cagtgtgctg ttataagtac tcttccatac cataacactg
     2221 tatatataac aatgtgttta tatacagtgc atctaatact acgcccgaaa aaagcacatc
     2281 gtcaacatga ttttttgtgg ttttttcact tcctgcggac aaccttagat atgccatgtt
     2341 aaaacagaaa tataatctac gagctcaaca tgagacatac tggagacacg gtaattttta
     2401 tggcttggca caccgtaaag gacacactaa aactgaccgg tcgttcgcga tcccagctgt
     2461 accgggacat gaaaaaaggc cttgtcagtt accgcacagg gaaagatgga cggcgagaat
     2521 tcgacaccag cgagctgatt cgggcttacg gtgaactgcg gacggaggag acagtcaaaa
     2581 ggcacaaaaa gggacagcaa gaaattgacg atactccagc tgaacacccg caactgaaag
     2641 agatacaaca gcagttaact gccctgcagc agacggtcac attgatgttg gaggataaaa
     2701 gcactcgcga agccgaactc cggcaacacg atgaggagcg gacacagctg ctggccgaaa
     2761 tcagccgact gaatgaagat ctggagcagg aaaaaaaacg cagcctctgg tccaggttgt
     2821 tccgacgtga tattgaggag aaaaatcgtg aaaaatgaca gtttggagaa cgtcattcat
     2881 gaagcggata cacgggagaa gcatctgttc agatactggc gatggtgtct gaagtggggg
     2941 atttggttta tgctggcgtt gtacttgctc tggtgctatg tcaccagctt ctgacaaacg
     3001 atttaccata acgtcccatt acgcgcaccg cgaaaacggc ctcagggcgt ttctgcgcct
     3061 tcagccacaa aagagcacgt cttaccccca gaaaaccgcc cggaatcgct ctggtgagtt
     3121 ttacgggggt gaatgactat gcaccgtagt gaataccacg ctgtttcgcc ctgaactccc
     3181 cacgaaaccg acgaaagtgg caatcagcga cgcggcgccc gcggacgcga ccaggtaaat
     3241 tccgcctgct gccgctcctc ctcacgcgca gatcgctgcc gttctgcctg gcgctgcgat
     3301 tcgatctgtg cacgagccct gttaaggaca atgtgcctgt tgggctgatt cagcttcttg
     3361 cctgacgctc tctcagcggc ggcaacagca tcgtcgagaa atcttttctc ggcgggcgtc
     3421 aggtttatca gtcgtttcat ggcgatctcc ggtcagaata actctgcaca gtctgccaga
     3481 cacggcgaac ctcatgaaga acctgcagcc gggaacagcc ctgagaaacg gtgaacggga
     3541 acggatttta atccggtgcc gtgaagcgtt ggcggggtat cggggcgcag ccctgagata
     3601 tcgttttggt gccggcatcg tgatggcggt actccaaaaa cacatcttgt cccgctttca
     3661 gtttctgaat ttgcgggttc agggcgcagc gccggaccag tcacgtagcg ctagcggagt
     3721 gtatactggc ttactatgta ggtacggatg agggtgtcag tgaagtgctt tatgtggcag
     3781 gagaaaaaag gcggccccgg tgcgtcagca gaatatgtga tacaggagat attccgcttc
     3841 ctcgctcact aactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     3901 acggggcgga gatttcctgg aagatgccaa gaagatactt aacagggaag cgatagggcc
     3961 gcggcgaagc cgtttttcca taggctccgc ccccctgaca agcatcccga aatctgacgc
     4021 tcaaatcagt ggtggcgaaa cccgacagga ctttaagata ccaggcgttt ccccctggtg
     4081 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcact ccgctgttat
     4141 ggccgcggtt tctcattcca cgcctgacac tcggttccgg gaaggcagtt cgctccaagc
     4201 tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgcctgttcc ggtaactatc
     4261 gacttgagtc caacccggaa agacacgaca aagcgccact ggcagcagcc actggtaagc
     4321 aggttgtgcg agagatgtat taagagtact tgaagtgatg gccagactgc ggctacactg
     4381 aaaggacata tttggtgaca tgtctcgcac aagactgtta cctggttcag aaacttgccg
     4441 gtttctgatc cttcgaaaaa ccaccttgcc gggtggtttt ttcgtttata gagcaagaga
     4501 ttacgactca tcgcaaaaga tctcaagcag atcatttatg gatcgttacc tattcgtacc
     4561 actgtcgagc gtaacgcccg ttactgtgga tgttggagaa actgtaccac ctccagtatc
     4621 tcggacgtta ttcacttggc atgccgcgag aatcatcgat actatgacga ttcagactca
     4681 acaaattcaa aacgtcattg aaacccttaa aaaaggcgca gatgaggctg tgagcattgc
     4741 caaactcggt attcgcgagg ccgaccacgg tgtacagcaa gttatagagg cgcagtgtgc
     4801 tatccaaggc attcgcgatg ctgttgaacg gatcagtggt atgagccagc aaatggcagc
     4861 cgcttcagaa gagcaggcta gtgtcgctga agatattgct cggcagatta ataacgtagc
     4921 gggaacggtt gagagaacag cgaagaatgc taactccgcc gtgattcgtg gccgcgaact
     4981 tgaaagtaca tcccgtggtt tcaactcgtt ggttgaacgg ttcaacaggt agtgacctta
     5041 tgactatttt ggtgaattag gtttatatat gagtgaaaga taccgtgcag cggtggatgc
     5101 ggcagctatt ttctcggaaa ccgata
//
