LOCUS       CP052708                5126 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0078 plasmid pB16KP0078-2,
            complete sequence.
ACCESSION   CP052708
VERSION     CP052708.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609866
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 20:00:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,564
            CDSs (total)                      :: 5,436
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 60 of 116
            Pseudo Genes (incomplete)         :: 50 of 116
            Pseudo Genes (internal stop)      :: 23 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0078"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0078-2"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            join(5068..5126,1..178)
                     /locus_tag="HJW78_27690"
     CDS             join(5068..5126,1..178)
                     /locus_tag="HJW78_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN36133.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(524..982)
                     /locus_tag="HJW78_27695"
     CDS             complement(524..982)
                     /locus_tag="HJW78_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJN36134.1"
                     /translation="MAWHTVKDTLKLTGRSRSQLYRDMKKGLVSYRTGKDGRREFDTSE
                     LIRAYGELRTEETVKRHKKGQQEIDDTPAEHPQLKEIQQQLTALQQTVTLMLEDKSTRE
                     AELRQHDEERTQLLAEISRLNEDLEQEKKRSLWSRLFRRDIEEKNREK"
                     /name="entry exclusion protein 1"
     gene            1268..1771
                     /locus_tag="HJW78_27700"
     CDS             1268..1771
                     /locus_tag="HJW78_27700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJN36132.1"
                     /translation="MPGFNYGGNSVGDGTNWSSERGSDPTTPGGGDNGHAGDHSSSGSS
                     SSTVEHTPWGDLTTDAAGYAYMNGIKLTADNSQLAYVSPTHLVRVLNSLLDRSYMKPEP
                     GEHSGVTGVPATSPSRVTLADNALKNAQSKAQDAAAALSKANAAISQAQSVRGRCGLSI
                     TPSR"
                     /name="hypothetical protein"
     gene            complement(1748..3439)
                     /locus_tag="HJW78_27705"
     CDS             complement(1748..3439)
                     /locus_tag="HJW78_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="QJN36135.1"
                     /translation="MSERYRAAVDAAAIFSETDKSGNITYVNEQFCDISGYSAQELIGQ
                     NHRILNSGYHPPEFFIDMWKKISRGQVWKGEICNRKKDGSLYWAESTIVPMYDDASQRV
                     QKYVSIRFDVTEKRKFLETLQWQAEHDELTGLPNRFLLSKALDQAIVKAKSQPSTVAVG
                     VLDLDGFKQINDGYGHEMGDRLLVAVADRLKHSMHIEDTVARFGGDEFVLILGVRDPKV
                     LESALQRLLAALSAVYIIDGIDINISASIGVTLYPNDNENADTLLRHADQAMYKAKQSG
                     RNCFHLFDVSKDKMDKSAFDTVIRVRQALHDGELCLHYQPKISLSSGAVIGFEALLRWQ
                     HPRDGLILPQYFIPLIEQSDLIVEIGEWVIDQALSQIEQWADLGHSWSVSVNIAALHLK
                     KENFVKSLKFLLDSHPNVLPQMLDIEITESVVIEHLPHVTQCLIACQDLGVTFSLDDFG
                     TGYSSLSYLKQLPTQSIKIDKSFIRDILIDKDSLRLTKAIIGLAKSFNREVIAEGVETV
                     EHEVLLMNLGCDVAQGYGIANPMPVEKVLSWVAKFVPAHLSRGGNR"
                     /name="EAL domain-containing protein"
     gene            complement(3476..3832)
                     /locus_tag="HJW78_27710"
                     /pseudo=""
     CDS             complement(3476..3832)
                     /locus_tag="HJW78_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754514.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            3852..3962
                     /locus_tag="HJW78_27715"
     CDS             3852..3962
                     /locus_tag="HJW78_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJN36136.1"
                     /translation="MILAACQVNNVRDTGGGTVSPTSTVTGVTLDSGTNR"
                     /name="colicin release lysis protein"
     Typing          4660..4830
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="98.0"
                     /evalue="2.63e-66"
     Typing          complement(4173..4693)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.702"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgccgctgag agagcgtcag gcaagaagct gaatcagccc aacaggcaca ttgtccttaa
       61 cagggctcgt gcacagatcg aatcgcagcg ccaggcagaa cggcagcgat ctgcgcgtga
      121 ggaggagcgg cagcaggcgg aatttacctg gtcgcgtccg cgggcgccgc gtcgctgatt
      181 gccactttcg tcggtttcgt ggggagttca gggcgaaaca gcgtggtatt cactacggtg
      241 catagtcatt cacccccgta aaactcacca gagcgattcc gggcggtttt ctgggggtaa
      301 gacgtgctct tttgtggctg aaggcgcaga aacgccctga ggccgttttc gcggtgcgcg
      361 taatgggacg ttatggtaaa tcgtttgtca gaagctggtg acatagcacc agagcaagta
      421 caacgccagc ataaaccaaa tcccccactt cagacaccat cgccagtatc tgaacagatg
      481 cttctcccgt gtatccgctt catgaatgac gttctccaaa ctgtcatttt tcacgatttt
      541 tctcctcaat atcacgtcgg aacaacctgg accagaggct gcgttttttt tcctgctcca
      601 gatcttcatt cagtcggctg atttcggcca gcagctgtgt ccgctcctca tcgtgttgcc
      661 ggagttcggc ttcgcgagtg cttttatcct ccaacatcaa tgtgaccgtc tgctgcaggg
      721 cagttaactg ctgttgtatc tctttcagtt gcgggtgttc agctggagta tcgtcaattt
      781 cttgctgtcc ctttttgtgc cttttgactg tctcctccgt ccgcagttca ccgtaagccc
      841 gaatcagctc gctggtgtcg aattctcgcc gtccatcttt ccctgtgcgg taactgacaa
      901 ggcctttttt catgtcccgg tacagctggg atcgcgaacg accggtcagt tttagtgtgt
      961 cctttacggt gtgccaagcc ataaaaatta ccgtgtctcc agtatgtctc atgttgagct
     1021 cgtagattat atttctgttt taacatggca tatctaaggt tgtccgcagg aagtgaaaaa
     1081 accacaaaaa atcatgttga cgatgtgctt ttttcgggcg tagtattaga tgcactgtat
     1141 ataaacacat tgttatatat acagtgttat ggtatggaag agtacttata acagcacact
     1201 gcggaaacgg caggcaacaa accgcacacc tgatttctaa taaaaataaa acaaaaaggg
     1261 aaaaattatg cctgggttta attatggtgg taacagtgta ggggatggta ctaattggag
     1321 ttctgagcgg ggttctgacc ccactacgcc agggggcggg gataatggtc atgctggaga
     1381 tcattcttca tctggtagtt cgtcatcaac tgttgaacat accccttggg gagacctgac
     1441 aaccgacgct gccggctatg cttatatgaa tggtattaaa ctgacggcag ataactcgca
     1501 acttgcttac gtttctccaa ctcatcttgt gcgagtactt aattctctgc tcgatcgttc
     1561 ttacatgaaa cccgagccag gtgaacattc cggtgtgacg ggggtgcctg cgacatcccc
     1621 aagccgtgta accctcgcag ataacgcgct gaagaatgca caatcaaaag cccaagatgc
     1681 cgcagctgct ctgtcgaaag caaatgcggc tattagtcag gcgcaatcag ttcgtggacg
     1741 gtgcggttta tctattaccc cctctagata aatgagcagg gacaaacttc gccacccaag
     1801 atagcacttt ttctaccggc atggggttcg caataccata gccttgcgct acatcacaac
     1861 ctaggttcat cagcaacact tcgtgctcaa ccgtttccac cccttcagca ataacctcgc
     1921 ggttaaacga tttcgctagg ccgatgatcg ccttagtcag cctcaagctg tctttatcga
     1981 tgagtatgtc gcgtataaaa gatttgtcta tcttgattga ctgcgtgggc agttgtttaa
     2041 ggtagctcag cgatgaatac ccggttccaa agtcgtccaa cgaaaatgtc acacccagat
     2101 cctggcaagc aatgaggcac tgagtgacat gtggtaagtg ttctatcacc actgattcgg
     2161 taatctcaat atccaacatc tgcggcaaaa cattagggtg gctgtccagc aaaaacttta
     2221 gggactttac aaagttttct tttttcaaat gtaatgctgc aatgtttaca ctcacagacc
     2281 acgaatgacc caagtccgcc cattgttcta tttgactgag cgcctgatct atgacccact
     2341 cgccgatctc cacgatcaga tcgctctgct caatcaacgg tataaaatac tgcggtaata
     2401 taagtccatc tcgcggatgc tgccaacgca atagtgcttc aaaaccaatc actgccccac
     2461 tgctcaaact tatttttggt tgataatgca agcataactc accatcatgt aacgcttggc
     2521 gaacccgtat tacggtatca aaagctgatt tatccatctt atcttttgat acatcaaaca
     2581 aatggaaaca gttacggcca ctttgtttgg ccttatacat agcctgatcg gcatgacgta
     2641 gcagtgtatc ggcattctca ttatcgtttg gatagagcgt cacaccgata ctggcggaaa
     2701 tattaatgtc tatgccatcg atgatgtaga cggcggaaag ggcagctaaa agcctctgca
     2761 gggcgctttc caacaccttg ggatcccgca cacccaaaat cagcacgaac tcatcgcctc
     2821 caaaacgggc tacggtatct tcgatatgca tactatgttt caggcgatca gccaccgcca
     2881 ctaacagacg atcacccatc tcgtggccat agccgtcgtt aatctgctta aacccatcga
     2941 gatcaagcac gcccacggca accgtgctgg gctggctctt tgctttgaca atagcctgat
     3001 caagcgcttt ggacagcaaa aaacgatttg gaagacccgt cagctcgtcg tgctcagcct
     3061 gccattgcaa ggtttccaga aacttccgct tttcggtcac atcgaaacga atggaaacgt
     3121 atttctgcac ccgctggctg gcatcatcgt acatgggtac aatggtgctc tctgcccaat
     3181 acaggctgcc atccttttta cggttgcaaa tttcaccttt ccatacctgc ccacgtgaaa
     3241 tttttttcca catatcaata aagaactcag ggggatggta ccctgagttt agtatgcggt
     3301 ggttttgccc aatcaactcc tgtgcactat agccagaaat atcacagaac tgttcattga
     3361 cataggtgat atttccggat ttatcggttt ccgagaaaat agctgccgca tccaccgctg
     3421 cacggtatct ttcactcata tataaaccta attcaccaaa atagtcataa ggtcactacc
     3481 tgttgaaccg ttcaaccaac gagttgaaac cacgggatgt actttcaagt tcgcggccac
     3541 gaatcacggc ggagttagca ttcttcgctg ttctctcaac cgttcccgct acgttattaa
     3601 tctgccgagc aatatcttca gcgacactag cctgctcttc tgaagcggct gccatttgct
     3661 ggctcatacc actgatccgt tcaacagcat cgcgaatgcc ttggatagca cactgcgcct
     3721 ctataacttg ctgtacaccg tggtcggcct cgcgaatacc gagtttggca atgctcacag
     3781 cctcatctgc gcctttttta agggtttcaa tgacgttttg aatttgttga gtctgaatcg
     3841 tcatagtatc gatgattctc gcggcatgcc aagtgaataa cgtccgagat actggaggtg
     3901 gtacagtttc tccaacatcc acagtaacgg gcgttacgct cgacagtggt acgaataggt
     3961 aacgatccat aaatgatctg cttgagatct tttgcgatga gtcgtaatct cttgctctat
     4021 aaacgaaaaa accacccggc aaggtggttt ttcgaaggat cagaaaccgg caagtttctg
     4081 aaccaggtaa cagtcttgtg cgagacatgt caccaaatat gtcctttcag tgtagccgca
     4141 gtctggccat cacttcaagt actcttaata catctctcgc acaacctgct taccagtggc
     4201 tgctgccagt ggcgctttgt cgtgtctttc cgggttggac tcaagtcgat agttaccgga
     4261 acaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     4321 tgccttcccg gaaccgagtg tcaggcgtgg aatgagaaac cgcggccata acagcggagt
     4381 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa
     4441 cgcctggtat cttaaagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
     4501 ggatgcttgt caggggggcg gagcctatgg aaaaacggct tcgccgcggc cctatcgctt
     4561 ccctgttaag tatcttcttg gcatcttcca ggaaatctcc gccccgttcg taagccattt
     4621 ccgctcgccg cagtcgaacg accgagcgta gcgagttagt gagcgaggaa gcggaatatc
     4681 tcctgtatca catattctgc tgacgcaccg gggccgcctt ttttctcctg ccacataaag
     4741 cacttcactg acaccctcat ccgtacctac atagtaagcc agtatacact ccgctagcgc
     4801 tacgtgactg gtccggcgct gcgccctgaa cccgcaaatt cagaaactga aagcgggaca
     4861 agatgtgttt ttggagtacc gccatcacga tgccggcacc aaaacgatat ctcagggctg
     4921 cgccccgata ccccgccaac gcttcacggc accggattaa aatccgttcc cgttcaccgt
     4981 ttctcagggc tgttcccggc tgcaggttct tcatgaggtt cgccgtgtct ggcagactgt
     5041 gcagagttat tctgaccgga gatcgccatg aaacgactga taaacctgac gcccgccgag
     5101 aaaagatttc tcgacgatgc tgttgc
//
