LOCUS       CP052568                3479 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain A16KP0127 plasmid pA16KP0127-3,
            complete sequence.
ACCESSION   CP052568
VERSION     CP052568.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609860
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:14:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,439
            CDSs (total)                      :: 5,315
            Genes (coding)                    :: 5,207
            CDSs (with protein)               :: 5,207
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 45 of 108
            Pseudo Genes (incomplete)         :: 58 of 108
            Pseudo Genes (internal stop)      :: 21 of 108
            Pseudo Genes (multiple problems)  :: 16 of 108
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="A16KP0127"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pA16KP0127-3"
                     /geo_loc_name="South Korea: Seoul"
                     /lat_lon="37.57 N 126.98 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(join(3426..3479,1..498))
                     /locus_tag="HJW72_27165"
     CDS             complement(join(3426..3479,1..498))
                     /locus_tag="HJW72_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QJM50680.1"
                     /translation="MVLELSNTEMLLLIHLISFIHFADTPAFPSISTLAGRINQHPRTV
                     QRTINKLISKGFLKRKIRAKSVNDKGLSNLYSFEPLIEKLLISISNNEDQYEELQKEQG
                     ERDLKTVDQLVRIAAAGGGLRLSASTKTVDQLVRIAAAAGNSITPNKAKLVLFDLESKT
                     VDELVRIAAAGKGCVLFELE"
                     /name="helix-turn-helix domain-containing protein"
     gene            816..1046
                     /locus_tag="HJW72_27170"
     CDS             816..1046
                     /locus_tag="HJW72_27170"
                     /inference="COORDINATES: protein motif:HMM:NF015947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="QJM50676.1"
                     /translation="MTTAIGKWGNGAALRIPQPFMKQLGLSIGDVVNFRVTERDLVITK
                     AGPTLEELLGQCTAENRHHEYLCDSQGKEML"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            1043..1381
                     /gene="mazF"
                     /locus_tag="HJW72_27175"
     CDS             1043..1381
                     /gene="mazF"
                     /locus_tag="HJW72_27175"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="QJM50677.1"
                     /translation="MTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFA
                     VVCPITNQIKGYPFEVPVDGTTKTTGVILADQVKSLDWKARAARTVDSVSGETVTTVVD
                     MVSKIIKA"
                     /name="mazF"
     gene            complement(1512..1645)
                     /locus_tag="HJW72_27180"
                     /pseudo=""
     CDS             complement(1512..1645)
                     /locus_tag="HJW72_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1670..2094)
                     /locus_tag="HJW72_27185"
                     /pseudo=""
     CDS             complement(1670..2094)
                     /locus_tag="HJW72_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /name="plasmid replication initiation protein"
     gene            2051..2242
                     /locus_tag="HJW72_27190"
     CDS             2051..2242
                     /locus_tag="HJW72_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJM50678.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2242..2643
                     /locus_tag="HJW72_27195"
     CDS             2242..2643
                     /locus_tag="HJW72_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="QJM50679.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHLSGQVSEQRDTAFADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="DNA-binding protein"
     Typing          complement(1545..2003)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.526"
                     /coverage="90.0"
                     /evalue="1.69e-147"
ORIGIN
        1 ctcatcaaca gtcttggact caagatcaaa cagaactaac tttgctttat tgggggttat
       61 cgagttacct gcggctgcgg caatccgcac gagctgatcc actgtttttg tggatgcact
      121 taaacgtaaa ccacctcctg ctgcagcaat ccgaactaat tgatctactg ttttcaagtc
      181 tctttctcct tgttctttct gaagttcttc atactggtct tcgttgttac taattgatat
      241 taatagcttt tctatcaaag gttcaaagct gtaaagattg ctcaagcctt tgtcattgac
      301 tgattttgcg cggatttttc tcttcaagaa ccctttagat attaatttgt ttatggttcg
      361 ctgcacggtt cttgggtgtt ggttgatcct acctgctagt gtagaaatgc ttgggaatgc
      421 tggggtgtct gcaaagtgaa tgaaagagat tagatgaatg agtaacagca tttcggtgtt
      481 actcaattct agaaccatct gtttttcaag caataaatta ggaacagttg tccaccctgc
      541 ttcctctagg atgctatatc cccacctgct tgctaagatc gctggtattt ggttgcggtt
      601 tatttttttt tctttgtaaa tcaatttgtt atcgccactt agttaaaaaa aaattttttt
      661 tgccattggt aactacttaa acaaataagt atttatttgt ttaagtagtt accccttttt
      721 agggtggcat tggtgccacc cttcaaactg aactcaaaga ctatacaata caaatattgt
      781 ttacacattg tttatccatc tgctttggag gtattatgac tacggctatt ggtaaatggg
      841 gtaatggcgc tgctttgcgt atcccgcagc cgttcatgaa gcagcttggg cttagtatcg
      901 gtgatgttgt taatttccgc gtcactgaaa gggatttagt aattactaaa gctggcccga
      961 cgcttgaaga actgctcgga cagtgcactg cagaaaatcg ccatcacgag tatctatgtg
     1021 actctcaagg taaggagatg ctgtgacgac atattgtcca gccagaggcg atgtcattct
     1081 tttagatttt aaccctcaat cagggcatga gcaggcaggg aaaaggcctg ctcttgtcgt
     1141 ttctgatgat ttgtttaacc aagtcactgg gtttgctgtt gtttgcccca taactaatca
     1201 gataaaaggg tacccttttg aagttcctgt tgatgggact actaaaacaa caggggttat
     1261 cttggctgat caagtgaagt ctttagactg gaaggccaga gcggcacgta ctgttgatag
     1321 cgtttccggc gagacagtaa ctacagttgt tgatatggtt tcaaaaatta ttaaagcata
     1381 aaaaaagacc ggcataagcc ggtcttttca ggccgaaaat gttaccagca ttttcggcct
     1441 tcggtgactg ctctaaaggg gaacagtaac caatattgtt tatcaagtgt aactgcttac
     1501 tgttacagca atcaagattt tctttccccc gtagagcgac cgatggctac tgccaatggc
     1561 gctttggcgt gcttgttcgg gttggactca agttgatagt taccgaatta gcgcagtagt
     1621 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc taaccggaac
     1681 tgagtgtcag gcgtggaatg agataaacgc ggccataaca gcggaatgac accggtaaac
     1741 cgaaaggcgg gaacaggaga gcgcacgagg gagccatcag ggggaaacgc ctggtatctt
     1801 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc aaattttgtg atgcttgtca
     1861 gggggggcgg agcctatgga aaaacggccg ttgtgcggcc ttgtccctct tcgtgctgtc
     1921 tgtcctatgc cgttatgtgt gcgttgcgac cgctcgccgc agccgaacgg caggagcgta
     1981 gcgactgagt gagcgaggaa gcggaaaaga gactggtttt acactgagca ctgacgctct
     2041 gaggcttctt atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagca
     2101 tcagtctctg ctcctgctgg agaagcttaa cgagcttgat cttgatgctg aggccgacct
     2161 ttgtgagaaa ctccatgatg atgcggagca tcttttccgt acactgtcct cgagactgga
     2221 tgcattacag gatggtaact gatggccaga gtgtctatta gtgaggcagc ccgattgacg
     2281 ggtaagagta gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc
     2341 ggtgaacgta atgcaaaaat ggtagatacg tcagagcttt tgcgtgtctt tggttccttt
     2401 gagcaaccta agggtgaaca tctttctgga caggtctctg aacagcgtga tacagccttt
     2461 gctgaccaga atgaacaggt tattcgtcat ctgaaacagg aaattgaaca cctgaacacc
     2521 ttacttttgt ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa
     2581 aatcaccagg caaaattaac tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc
     2641 tgacaccgtc atgcttcagt tttcctctcc ggccattccg gggagttcct gaaccttccc
     2701 aaaaatcgtc agatctctga acgcacctca ccacttaaaa tacccacaca ccacgcaaaa
     2761 ttaaaatttt gcagataact cattaatatc agtttgttag gtgttattaa attacgaatt
     2821 attaccttct gtaaggtaaa tccaagtcgc cggcaagtcg tatacgactt gccggcgact
     2881 tgtcatagag tcatcgacaa gtcactccag actatcagta acttatgacg acttgactct
     2941 agagttgagt cgttcatttg gtgttttttt gaccttggtg actctagagt caagtcacga
     3001 gtcgaatcgc cggtgaatcg tgtgctaagt cgtccggtga cttgccatca cgtcattgcc
     3061 ggctgtcgct gaactggatt cttgaacgtg ctgaaaacgg gttttccggc tgcgccggca
     3121 acgcctcacg gccacgcaat aaattgcgtc cccgttcacc gttttcggat cagaaaaacg
     3181 ccggaaacag gacttaccct ggctgaatgg catgaatgcc cgcgctatgc acttgcatgc
     3241 atactcatgt atgccgtaaa aacagcccct gcgcgtttct ggcaggtttt cgggtggttt
     3301 gttgcctgtt ttgccggttt cccgtcagaa acgccctgaa gccgttttag cggtgcacgt
     3361 aatagggctt tatgttaaat taagactggt ttgatgttta aacttatagt gatcttttta
     3421 gtgtgttact ccaattcgaa tagtacacac ccctttcctg ctgcagcaat tcgaactag
//
