LOCUS       CP052555                9294 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain A17KP0038 plasmid pA17KP0038-4,
            complete sequence.
ACCESSION   CP052555
VERSION     CP052555.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609864
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:13:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,564
            Genes (coding)                    :: 5,431
            CDSs (with protein)               :: 5,431
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 69 of 133
            Pseudo Genes (incomplete)         :: 67 of 133
            Pseudo Genes (internal stop)      :: 16 of 133
            Pseudo Genes (multiple problems)  :: 17 of 133
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="A17KP0038"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pA17KP0038-4"
                     /geo_loc_name="South Korea: Seoul"
                     /lat_lon="37.57 N 126.98 E"
                     /collection_date="2017"
                     /collected_by="Kor-GLASS"
     gene            1468..3432
                     /locus_tag="HJW76_28375"
     CDS             1468..3432
                     /locus_tag="HJW76_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QJM24395.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3432..4163
                     /locus_tag="HJW76_28380"
     CDS             3432..4163
                     /locus_tag="HJW76_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QJM24396.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            4170..4700
                     /locus_tag="HJW76_28385"
     CDS             4170..4700
                     /locus_tag="HJW76_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM24397.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4728..4907)
                     /locus_tag="HJW76_28390"
     CDS             complement(4728..4907)
                     /locus_tag="HJW76_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QJM24398.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4976..5353
                     /locus_tag="HJW76_28395"
     CDS             4976..5353
                     /locus_tag="HJW76_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QJM24399.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(5415..5714)
                     /locus_tag="HJW76_28400"
     CDS             complement(5415..5714)
                     /locus_tag="HJW76_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QJM24400.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5704..5967)
                     /locus_tag="HJW76_28405"
     CDS             complement(5704..5967)
                     /locus_tag="HJW76_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QJM24401.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(6273..6692)
                     /gene="exc2"
                     /locus_tag="HJW76_28410"
     CDS             complement(6273..6692)
                     /gene="exc2"
                     /locus_tag="HJW76_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QJM24402.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            7095..8780
                     /locus_tag="HJW76_28415"
     CDS             7095..8780
                     /locus_tag="HJW76_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJM24403.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            8790..9047
                     /locus_tag="HJW76_28420"
     CDS             8790..9047
                     /locus_tag="HJW76_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QJM24404.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin"
     gene            9132..9281
                     /locus_tag="HJW76_28425"
     CDS             9132..9281
                     /locus_tag="HJW76_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QJM24405.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2095..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(91..669)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3433..4160
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg aaaaaaccac
       61 ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac cgtggtaact
      121 ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac tttggcgcac
      181 acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc agttaccaat
      241 ggctgctgcc agtggcgttt tgcgtgcttt tccgggttgg actcaagtga acagttaccg
      301 gataaggcgc agcagtcggg ctgaacgggg ggttcttgct tacagcccag cttggagcga
      361 acgacctaca ccgagccgag ataccagagt gtgagctatg agaaagcgcc acacttcccg
      421 caagggagaa aggcggaaca ggtatccggg aaacggcagg gttggaacag gagagcgcaa
      481 gagggagcga ttcatcggaa acggtgatga tctttaagtc ctgtcgggtt tcgccactcc
      541 tgactgattc atggttgagc caccggctcc cacagatgca ccgaaaaagc gtctgtttat
      601 gtgaactcag tcaggagggc ggagcctatg gaaaaacgcc acctgcgtgg ccttgccgtt
      661 ttgcctcaca tgttagtccc ctgcttattc acggaatctg tgggtaactt tgcatgtgtc
      721 cgcagcgccc gccgcagtct cacggccgga gcgtagcgac cgagtgagcg aggaggcgca
      781 atttccccgg cacctacaag acggacaccg gacgcgctga tgtggcatat tgtcccctga
      841 ttaagtcttt atattccctg ataataaatt accaggatag gtgaatcata tcggtttttc
      901 gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttacgga gaaatacagg
      961 tcatccccgc gagggcgtgg ctgacctcag ggggatatcc cctgcttacg ctggctggac
     1021 gccaccgacg cacggtctct ccccctcttt ctggctccgt ccggctggac gccggccacg
     1081 ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa atccacggtg
     1141 ctggacgcac ctggcgaagc agcaaagctg ctcctgattt tgcgggtggt cagccgctcg
     1201 ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt
     1261 ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt ttccgcatgg
     1321 ggcggaaaga ggctggcctc cgtggtgaaa gctgaacgca atcaccacgg acgtgaccgc
     1381 cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg ggggctgcac atcacgccgg
     1441 caaaccatcg tctgaaggag agagaacatg tttaatacgg attcgcttgc ctggaagtgg
     1501 cacattccgg agctggcccg gcagatttat ccgtgggtca gctggctgta ccaggagtat
     1561 tacggtctgg ccggggcttt cctagttatc ggcctgtttt tcggtctggc tgaactttgg
     1621 gccggacgga tgcgttttgt ggggctgagt cataaggagc gacgggagta tgccgatcgc
     1681 tataagcgcg ataataaacg gccagcgcca ttttcggtgc tggcctcgct ggattcgccc
     1741 gggcggatgg tcagggcacg tcgacatgtc tggaactacg cgtactggct gaccggcgcg
     1801 gccggtctgg cccttctggg ggggctggtt gtcgccgtgt ttatcgagag ctggggagaa
     1861 accgtcctgc gtccggacct ctacgcaaac gcagattatt acctgctgca gccggctaac
     1921 ctgttcggga ttgcctggtg cgcgggcggg ctggctgtcg ggattgtgtt ggcgcggctg
     1981 ctggcgttcc tgctgcttga cggtcatttt gcggctgctg acgaggcggt gaacgcgaaa
     2041 ctgaatcagg agagccggcg cagcagccag cggacaggag agatgaccga cgtgcgccac
     2101 ctgcatttcg gggagccggt accggttaat gcgctggccg atttttcctc ggagcaggcc
     2161 cggcaggagc aggcggtgtt tctgggtaag gatgagcagg gcaagccggt gctggttccg
     2221 cgtgatacct ggcgcaagac caacattcag atcctcggcc tgcccgggag cggcaagagc
     2281 gtgatggcca ccaacgcgct cattcgctgc gtacgcgatt tcggggacgc ggtggtgtac
     2341 ttcgacccca aaggggacgc atgggcgccg cacgtcttcc gggcgcactg tccggacttc
     2401 accctgctgg atctgcggcc ggggaaaccg gcgcagctga atctgttccg cgatctcgac
     2461 cagtacgcgc tgaaaaacct gctgattgcc gggcttaacc tgggcgagac cggcgacgtg
     2521 gcggaccact accgcatcag cgagcagaaa gcggcgaagc tgattgcgga gcagtttccg
     2581 cagggggcaa acattcagca ggtgctggcg gcggcgtacg cgctgccgga ggccctgaaa
     2641 aaggacgtga aggggttgat cactaggctg gaaaacgtcg ctgacctgag cgtcctgcag
     2701 acggacagtg gcatcgatgt ggcggggatt atcaacggcg gcggctgcct gtacgttatc
     2761 ggctcgatgg acgacgaggc ggtgatccgt gtgcaaaaaa tgctgtttgc ccgctgcgcg
     2821 caaatcatca ttgcgcggga cgagttccgg gagtggccac acgtcagcgt gatgctcgat
     2881 gaaattaaat atctgctgtc gaagtatgta ctgaacgcac tggggacgct gcgcagccgg
     2941 gactgcaatc tgttgctcgc gcaccagtcg ctgggcgatt tcgggcagtg cgggcaggat
     3001 ctgcccgctg aatttgtgaa aaccacggta ctggataaca cgccgatccg ctggttttac
     3061 cgggcggcca gcctggagtc cgcgcagtgg gcggcggggc agaccgggga aatcagggtg
     3121 gacgtggagc gccgccgtgc cagccgggag gcggggaacg tggagcatat cagcggggac
     3181 agcttcatcc agaaggaggc gcggccgctg tttgacgtga acaccctgca acacctgccg
     3241 gacgggttcg cggtcatgac cgggctgggt gtggcccgga tggcgttcag cagtccgctg
     3301 cgggttgagc gccgggaaat cccgttgcag cgtttcccgg tgctggcgaa aaccgatccc
     3361 ctggcggagt tccggccccc ggccgacggc ggcgggccga aagcggatga tgattttgcg
     3421 gggatttact gatggcgctg gagctgtaca acgtcagcca cgcgaaacga caggcgcgga
     3481 acgcggagaa aacccggctg accctgcgct ggttgcggga agagctgtgt tccacggcgg
     3541 agctggttgc gcggcggctg gggattgcgg cggtgcagcc ggtctaccgc ttcctcgaca
     3601 gcctggtggc gaaagggctg ctggtccgga ccaaatatcc ggtggacggc cgtcaggtca
     3661 gcgtgtgggg gctgacgccg catggcgtgg cgttctcgtt cgacgaggac gaaccgctgg
     3721 cggatgttat tccgtttcag ccgtcaaggg tgtcggcagc acatctgccg caccggctgg
     3781 ccgtgcaatc cctgcgcctt gatatggaag ccagaggggc caccggctgg cgcattctgc
     3841 accgtctgag tcttaaaggc atgaaagtgc cggacgccct ggcggagctg gacggtaaaa
     3901 cggtggcgtt tgaggtggag cgcaccgtca agagccgtcg ccggtatcag gaagtggtgt
     3961 ccgggtatct gttcaaccgc aaagctaacg gcatcgatga aatctggtac atctgcccgg
     4021 accgggcgac gcaggtcagg gtgcagcggg caatcctgtc ggtggatgaa atcgtgaacc
     4081 cacagaccgg cgaggcccgc aaaacggcag agctggatcg tgaacggttg tttgcgtgct
     4141 ttaaatttat gacaacggag tgagggatta tggcgctgta catggtattt attgaagacg
     4201 cggaggaccg gaacggcgag cgctgcatca tgccgaccag cgagcatctg gaagatatgg
     4261 tggtgctcgc ctggcagacc ggggcgtatg ttttctgttt tgtcgaccgg gcccagcatc
     4321 tggcgatgat gaaatccatc gggatgtatg acgagcggct caacagcgac gaaaaagcca
     4381 cgtacttttt cactggtaaa aatccgcaat gggaacggaa caaacccaca ggaatgaagc
     4441 tgtgtgatta cctctatcgc aaggtggagc ggttcgccat cgagaacggt gtgcaggtgg
     4501 ttgatgccgg gaagtgctca ccggagcaga ttaacgcgat gattgacgtg gtgagtgagc
     4561 ggcacgggga aagcgcggtg aacgagaagc tgaaagcgga gctgatgcgg gaatataaca
     4621 gccgctattt taacgtgccg gtacctgaac tgccggagga caaacctgcc cgccggtggt
     4681 ggccgttcgg caaaaaataa atgccagcac cggccatgcc ggtgctgtta ttcctccccg
     4741 agcttatccc gcatcacctg ccagagtgtt tccgcatcct cgtgtagccg ctcgcacatg
     4801 tcggccgggg tatccatatc cagctcattc agtttatcga gcaatagcag cgactgctcc
     4861 ttaataaatt tagccatatt gagggctgtt ttttcctgtt ttgtcatgat tctttccttc
     4921 cgggcatggt gtgcgcctgt caggtatact gtctcaaaaa tagcggggtg agacaatggc
     4981 gtgggtgacg gtgagacagg cgacggaact gacgggtaaa gcccgaagtt ccctgtatcg
     5041 ggatatggcg aaaggccgcg tatcataccg tacggaagct gatggcggcc gggtggtgga
     5101 taccagcgag cttatccggg tgtacggtga gttaagacag gatgagactc acgagcggga
     5161 cgagttgaga ctgtcgggtg agactgaaaa aatggcctcc gacgcgttga tcgcggagat
     5221 aaaagcactc agggaggagg tggccgggct acggcaggaa atgcagtcca tgagactgct
     5281 ggaacacaaa ccggagacag cagagacccc ggccggaaca ccgcgccggt ggtggccgtg
     5341 gggaaaacgc tgacgcgctg tgcttgtcca accggcgacg gccacgcaat gaattgcgtc
     5401 cccgtcaccg gttattagag aacaatatgg ggattctggc gctggccgac cacggccaga
     5461 acccggactt ttctggtctg gtcatcaaca tcatagagga catgctgtcc catcagactg
     5521 atccggcgaa cgtcctcgcc atagcgtgag tccacttcat agagtcgcgg gaagtcggcc
     5581 agtttgtccg ccgcagcgag aacccggtcg gtaacgttct gcgcatccca cagggatgaa
     5641 ttttccgcga tatagtcaaa cagcgtcgtt acatcctcga tggctccctg cttccattcc
     5701 acgtcatacg gcatcgcgca gttcctgata acggccatgc aggcgctggc ggagttgttc
     5761 cgtggtcata cccggacgcg tgtcagcacg ggcggcggtc actttggcgc gaacctgatc
     5821 ttcatactct gcctgggcca gttcttcgtt agccagtcgc tgcaacagcg cagctttgtc
     5881 cgggccacct tccggcagta tgcggggcag gctttcagcc agttcccgaa gaaagttggc
     5941 gttttcgtag tgtgtagtgt tgctcattgc ggagcctccc gggttaatgc ggcaatatga
     6001 aggaagttta gcagggggcg gtctgaaaga atatggtcgt gtcagtcagg ctgatgaagc
     6061 agaaagtcgg taaaaaaggg cttacggcat ccattttacg tcaaaacata tgccatgcag
     6121 ttgcgtgcat aggcatgcat taggataaaa tttatcgggc gcgtttccgg cagttttccg
     6181 ggggggttgt tgcctgtttt atcccgtagc cgccggaaac gccctgagcc cgtctgagcg
     6241 gtgcgcgtaa tgacgcgtta tggtaaatcc acttactgtc tgccttcata gccatcgaga
     6301 taaacagctg aaatggtgtc agccagtagc cttgattttt tcggtgtgag gtctttgctg
     6361 cggtaatcgg tacccgcaaa ttgtgttgtg ccttggagtg agtttaaaaa ttcctgactg
     6421 tgaaactgct gaatgcgttg ctgcacatca ctccgtgatt ttccggtcgc tttatccttt
     6481 gcgcccatat cccagaactg ccggaaaaaa ggcaccatca ttcgtgttgt gtcagctttc
     6541 tgggtggaaa aattgggatc aaaaccgtca tctgaagcat atacgtaatg ccgggtatgc
     6601 tgttccacgc tgccctttgg agcacaggca gtgagagttg ctgtcgctgt tatcatggcg
     6661 ataagacaca gtttgctgcc ggaacgtctc atgagcgtct ccttaccttt aattcttttt
     6721 tctcattgag ttatatatcc gcaggctatc ttatcatcag tttctgaccg tgttcagtga
     6781 ccgaacaacc cgaaccagac cccccgtttc ttttcctgtc ccggaggggg ctttctggta
     6841 tctcatataa tgatttcagt aaaacatact gaggccggag aacggcgccg tttgtgctgg
     6901 tttttgttgc atctggaggg gattgcgcat ggtgtgagtg gctgtttttt tgatcatttt
     6961 cacaaaaacg caaatttaca gacttgacat gaaaaacaag gcagcataat ttttatactg
     7021 tatataaaac aagtggttat atgtacagta ttttatttta gtttattgtt ttaaaagtca
     7081 aagaggaaaa gtatatgagt ggcggagacg gacgaggtcc gggtaattca ggtctgggac
     7141 acaatggtgg tcaggccagt gggaatgtga acggtacatc aggtaaaggt ggcccttcat
     7201 caggtggtgg tacggatcca aacagcgggc cgggctgggg tacgacgcat acacctaacg
     7261 gggatattca taactataac ccgggggagt ttggtaacgg cgggagtaaa cccggtggta
     7321 atggcggtaa cagcggcaat catagcggga gctctggtgg cggacagtct tcggccaccg
     7381 cgatggcctt cggtctgcct gccttggcca ctccgggggc tgaaggactg gctttatccg
     7441 tttccggcga tgcgttgtcg gccgctgttg ctgatgtgct ggctgccctg aaaggtccgt
     7501 ttaagtttgg tctgtggggg attgcgattt acggagtgct gccttctgag atagcaaaag
     7561 atgatccgaa aatgatgtca aaaatcgtga cgtcattgcc ggcagacacg gtaacggaga
     7621 cgccggtcag ctccctgccg ctggaccagg cgacggtcag cgtcactaaa cgtgtggcgg
     7681 atattgtgaa ggacgagcgg cagcatattg cggttgtcac cggccggcca atgagtgttc
     7741 ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt cagtgtgtcg attccgggtc
     7801 tcccgtctct gcaggtgagc gtacctaaag gtgttccggc cgcgaaagcc ccgccaaaag
     7861 gcattattgc tgaaaaaggt gattcacgtc cggctggttt tacggccggt ggtaactccc
     7921 gtgaagccgt tattcgtttc ccgaaagaga gcggacagaa accggtctat gtgtcggtga
     7981 cggatgttct tactccggca caggtaaaac agcgtctgga ggaagaaaag cgtcgccagc
     8041 aggcatggga cgctgctcac ccggaagagg ggctgaaaag agagtatgat aaagcgaaag
     8101 ctgagctgga tgctgaagat aaaaatattg cgaccttaaa cagtcgcatt gcatcgacag
     8161 agaaggcgat ccccggtgca agggctgctg ttcaggaagc cgataaaaag gtaaaagagg
     8221 cagaggcaaa taaagatgat tttgtgactt ataacccgcc tcatgaatat ggctccgggt
     8281 ggcaggatca ggttcgctat cttgataagg atattcagaa tcagaatgag aaattaaaag
     8341 ctgcccagac atctttaaac gaaatgaatg aatccttatc cagggataag gctgcgcttt
     8401 ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca gaaaataaat
     8461 taaatgagga aaagaaaaaa cctcgtaagg gaactaaaga ttacggccat gattattttc
     8521 ctgatcccaa gactgaagat attaaggggt tgggagagtt gaaagagggt aaacctaaaa
     8581 cccctaaaca aggtggtggt ggtaagcggg cccgatggta tggtgataaa aagcgtaaaa
     8641 tttatgaatg ggattcccag cacggtgagc ttgaagggta ccgcgccagt gatggcgaac
     8701 acctcggggc attcgatcca aaaacgggta agcaggttaa agggccggat ccaaaacgaa
     8761 acattaaaaa atatctttaa gaggtaaata tggggcttaa attacatatt cattggtttg
     8821 ataagaaaac cgaagagttt aaaggtggtg aatactcaaa agacttcggt gatgatggtt
     8881 ctgtcattga aagtctgggg atgcctttaa aggataatat taataatggt tggtttgatg
     8941 ttgaaaaatc atgggtttcg atattacagc cacactttaa aaatgtaatc gatattagta
     9001 aatttgatta ctttgtatcc tttgtttacc gggatggtaa ctggtaagca aaagttatca
     9061 aggatgattt gaaatacaga cttccggtgt tcagggatga ataccggggt ttttaaatac
     9121 agggaaggta tatgaaaaag gtaaaggttg tttttctttt tatattgatt ttttcaggct
     9181 ttcttctggt cgcatgtcag gcaaactata tccgggatgt tcagggtgga acggtggcac
     9241 catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca aagg
//
