LOCUS       CP052550                3511 bp    DNA     circular BCT 04-MAY-2020
DEFINITION  Klebsiella pneumoniae strain B16KP0089 plasmid pB16KP0089-4,
            complete sequence.
ACCESSION   CP052550
VERSION     CP052550.1
DBLINK      BioProject: PRJNA625837
            BioSample: SAMN14609867
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Built-in CTX-M extended-spectrum beta-lactamase gene in Klebsiella
            pneumoniae ensuring stable propagation of the multidrug-resistant
            pathogen in clinical settings
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Yoon,E.-J., Gwon,B., Liu,C., Kim,D., Won,D., Park,S.G., Choi,J.R.
            and Jeong,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2020) Research Institute of Bacterial Resistance,
            Yonsei University College of Medicine, 211, Eonjuro, Gangnam-Gu,
            Seoul 06273, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/27/2020 22:13:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,385
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 47 of 100
            Pseudo Genes (incomplete)         :: 48 of 100
            Pseudo Genes (internal stop)      :: 19 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B16KP0089"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pB16KP0089-4"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.66 N 126.77 E"
                     /collection_date="2016"
                     /collected_by="Kor-GLASS"
     gene            complement(134..475)
                     /locus_tag="HJW79_27510"
     CDS             complement(134..475)
                     /locus_tag="HJW79_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QJM18975.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            760..1071
                     /locus_tag="HJW79_27515"
     CDS             760..1071
                     /locus_tag="HJW79_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM18974.1"
                     /translation="MRRAEMAYERGGDFLEDARKILNREAIGPRRSRFSIGSAPLTSIT
                     KSDAQISGGETRQDYKDTRRFPLAAPSCALLFLPFGLPVSFRCYGRVYLIPRLTLSSG"
                     /name="hypothetical protein"
     gene            complement(1671..1853)
                     /locus_tag="HJW79_27520"
     CDS             complement(1671..1853)
                     /locus_tag="HJW79_27520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QJM18971.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            complement(2248..2463)
                     /gene="mbeD"
                     /locus_tag="HJW79_27525"
     CDS             complement(2248..2463)
                     /gene="mbeD"
                     /locus_tag="HJW79_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QJM18972.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="mbeD"
     gene            complement(2470..2967)
                     /locus_tag="HJW79_27530"
     CDS             complement(2470..2967)
                     /locus_tag="HJW79_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QJM18973.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="mobilization protein"
     gene            complement(2948..3508)
                     /locus_tag="HJW79_27535"
                     /pseudo=""
     CDS             complement(2948..3508)
                     /locus_tag="HJW79_27535"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(561..734)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          701..1222
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..144)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.25"
                     /coverage="83.0"
                     /evalue="2.52e-11"
     Typing          complement(141..454)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
     Typing          complement(2133..3511)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggcgacgca tcaatcagct cccggacttc ttccggcttc ccctgcagga cgctggcacc
       61 ttcacgctgc cggtctttac ccagaagata atccacagga ccggcgcccc cgccgcgacc
      121 ccggggatgg aatttaacaa tcatcgtccg gccctttttc cagtacggca tgccgcaacc
      181 gttccagacc ggcatcgatc gccatcagcg ctgccacgat ctgcacccgg tcatgaccgg
      241 caccaccggc gttcactttc cgggcgatct ggttcaggtt attgcccatg ccggcaagct
      301 gacgcagcag cgcgggcgaa atcgagggaa gcctgccggc gcgtgtcggt ttctcatcaa
      361 ggcaggtctg gcgcatccac gccgccagtt gttttccgtc gcaccgctca agcaaccgcc
      421 ggtgttcgtc ttccgtcacc cacatcgtga gcatcttgtt gcgtttatct gccagaatcg
      481 cctcctgata acgccgtgac ggacgggaaa gcctttcccg gtcggcgcgg ggttggacaa
      541 gccgcaggcg cgtcagtggg gtttcggggc gcagccctga accagtcacg tagcgctagc
      601 ggagtgtata ctggcttact atgttggcac ggatgaggat gtcagtgaag tgcttcatgt
      661 ggcaggagaa aaaaggcggc accggcgcgt cagcagaata tgtgatacag gatatattcc
      721 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
      781 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagcgata
      841 gggccgcggc gaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
      901 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      961 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     1021 tgttatggcc gcgtttatct cattccacgc ctgacactca gttccgggta ggcagttcgc
     1081 tccaagctgg actgtatgca caaacccccc gttcagtccg actgctgcgc cttacccggt
     1141 aactatcatc ttgagtccaa cccgagaaag cacgacaaag cgccactggc agcagccact
     1201 ggtaactggg atatacgaga gagagattca gaggtcttga agtatgggcc tgactgcggc
     1261 tacactgaaa gaacagtttt ggtatctgcg gtctcgtata cccagttacc tggttaagca
     1321 gttccccaac tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgttttca
     1381 gagcaagaga ttacgactga aacgacagga tcacaagaag atcattaact ccgagagtag
     1441 taactcccac catgtttgcc tatcaacaca cttaggaaac attacttgcg tttagttata
     1501 cgacttctag ctctccataa cagatcctca ttttctgctg atggatttcg acggtatcgt
     1561 tcctccgctt tccgtactcc atattcagga aacaccctga cagcaaccat ttgtataaag
     1621 acataacaca ccaaaagtaa aaataaaaaa ccaataaact caaacatata tcaccctctt
     1681 ttcttctcat ttctcatgcg tgtagccacc attattttat agttagcact cgtagggttc
     1741 tccatatatt tacgggtagc acgttcgacg gcagattcgg atgaactaaa aaaaagcata
     1801 aataatttaa atgcaataac ggcgactacc actacaccaa taaatgtaag catcactctt
     1861 actcctgtta tcaacattaa acataacatc tcttacccgc accgcgccgg aggcactcag
     1921 ggcgtttctg atggctgacg ggcaaaaggt ggtggcaagt acaccgaaaa ccaccagaaa
     1981 cgcacctgat ttattttacg catatgcatc acttattcac atcactgcat agccggtgat
     2041 ttcggttaaa aaagactctc tgtgtgctga aatcgcagaa aatggccatg atccccgtca
     2101 cgctcaggcc acctgagacg ggcgtttatc agtctccata ctccctgtca ccatgagata
     2161 atgtgcgttg ccgttccggt accagatctg ctgtcttctc tggttccttt tccgttgttt
     2221 gcccgtatcg ctgttgctca atctcattca gcctgtcgat aaccggcgca gctgctcgct
     2281 caaactctgc acctgcctgc tcaagcccga cacgcgctcg ctcagcgccg cgttctcccg
     2341 ttgcataaga ccagacatct tccgccattc ctcgaaggcg ctctcccact cgtccagcct
     2401 tctcgagtag tcctgctgta gctgctctaa cgcgctcagc aattgttttt ccagttccgt
     2461 cattctgctc tactcccgta ccagcttcac cgcgttccgc gtcttgttgt ccaccgtccg
     2521 gcccgtttcg gctttcatcc cctccggcag caccagaaac cgtccgttgc ggttctccag
     2581 ataggtcacg ccccacgtct gcatgtgcag tttcgacagc gtatctctct gctgcctgat
     2641 ttccgccagg ttcgccgcta tccggttccc ctggtaccac agcaccccgc tcaggctggc
     2701 gaacagcagc cctgacacca tggtgatggt cagccacgtc cgcccgacca ttcgcagcac
     2761 gctcagccgg ttcgactgca tcgccgcgtt catcccctgt tcgtgggcgc tgatggcgtc
     2821 gttgattctc ttcgcgcttg cgttcagttc cgatttgaca acgttctcgt gcttgctgaa
     2881 tgcggctttc agcctctctt cggtaagctg ctgctgtgct ttcgatttct cctcgaagtc
     2941 cctcgaggcc gttaaaagac tgctcataaa tggctcccct cagtcggata tttcgccccc
     3001 cgtccgggtc ggcaatgctg atactgtttt ttgtggttct gaccacctca aaaccggccg
     3061 ctgtgagcgc ccctgtgacg tcatcacgcg ttttcagctc tcctgatgag gctaacatca
     3121 gcaaaccccg tgtaatcgtc tctgcggctt cctgcttcgt ttctggcagt cctgaaggcg
     3181 tgaccagcgc ccgccggttc tccgggtcgt tcggatcatg tagccccagc ctgccgttga
     3241 ccactgtctg ccacgcatcg atgcgcggcc ggtcggcacg atcgtaatac ggctggaggc
     3301 gtttccccgt cagtaattcg gtattgggga tcagaaaatt cagctcaaga cgacccttgt
     3361 ctgcgtgttc cacccacaga atgctgtact ggtctttatc gagtccgggc atcagaaccc
     3421 gctcaaagct ggccatcagt ttttcacgct gacccggcgg caggtccgcc tccgcgaatg
     3481 acagcacccc ggaggtatat tttttcacat a
//
